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Article ; Online: Development and evaluation of Pirkle-type chiral stationary phase for flash chromatography.

Gonçalves, Layane / Cravo, Sara / Fernandes, Carla / Tiritan, Maria Elizabeth

2022 Volume 1675, Page(s) 463156

Abstract: Liquid chromatography is the most applied methodology for enantioseparation in preparative and semi-preparative scale; however, flash chromatography is seldom the first choice. This work proposes a new sustainable method to achieve pure enantiomers in mg ...

Abstract	Liquid chromatography is the most applied methodology for enantioseparation in preparative and semi-preparative scale; however, flash chromatography is seldom the first choice. This work proposes a new sustainable method to achieve pure enantiomers in mg scale. Herein, the functionalization of silica for flash chromatography columns with a suitable chiral selector, for subsequent quantitative enantioseparation of chiral compounds, is described. Accordingly, the Whelk-O®1 chiral selector was bonded to flash silica and packed into a reused solid phase extraction cartridge. For the evaluation of the enantioselective performance of the flash column, the enantiomers of a chiral derivative of xanthone were quantitatively enantioseparated with an average recovery of 70% and an enantiomer ratio (e.r.) of 99% and 97% for each enantiomer. Evaluation with the anti-inflammatory drug naproxen was also performed, resulting in an average recovery of 95% and 89% and 95% e.r. for each enantiomer. The flash column showed high stability and load ability, versatility, and good reproducibility.
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid ; Reproducibility of Results ; Silicon Dioxide/chemistry ; Stereoisomerism
Chemical Substances	Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2022-05-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2022.463156
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Article ; Online: Response.

Faias, Sandra / Cravo, Marília / Chaves, Paula / Pereira, Luisa

Gastrointestinal endoscopy

2021 Volume 94, Issue 1, Page(s) 202–205

Language	English
Publishing date	2021-07-16
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	391583-9
ISSN	1097-6779 ; 0016-5107
ISSN (online)	1097-6779
ISSN	0016-5107
DOI	10.1016/j.gie.2021.03.929
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Article ; Online: Comparative analysis of glucose and carcinoembryonic antigen in the diagnosis of pancreatic mucinous cysts: a systematic review and meta-analysis.

Faias, Sandra / Cravo, Marília / Chaves, Paula / Pereira, Luisa

Gastrointestinal endoscopy

2021 Volume 94, Issue 2, Page(s) 235–247

Abstract: Background and aims: Pancreatic cystic fluid (PCF) analysis is useful to distinguish between different cyst types and to guide management. The aim of our study was to compare the diagnostic accuracy of glucose level with carcinoembryonic antigen (CEA) ... ...

Abstract	Background and aims: Pancreatic cystic fluid (PCF) analysis is useful to distinguish between different cyst types and to guide management. The aim of our study was to compare the diagnostic accuracy of glucose level with carcinoembryonic antigen (CEA) in PCF for mucinous cyst diagnosis. Methods: We identified studies with PCF obtained by EUS before surgery, with cysts classified as mucinous and nonmucinous according to surgical specimens. A random-effects model was used for quantitative meta-analysis. Pooled sensitivities, specificities, and summary receiver operating characteristic (ROC) curve analysis were conducted. Results: For CEA, we included 31 studies with 5268 patients, of which 2083 were referred for surgery. For glucose, we included 4 studies with 345 patients, of which 275 were referred for surgery. Glucose performed better than CEA for mucinous cysts diagnosis (premalignant and malignant) with sensitivities of .90 (95% confidence interval [CI], .85-.94) and .67 (95% CI, .65-.70), specificities of .82 (95% CI, .72-.89) and .80 (95% CI, 0.76-0.83), and areas under the ROC curve of .96 and .79, respectively. Glucose had a higher sensitivity (90%), with uncommon false-negative results, making it an excellent biomarker to exclude a mucinous cyst. Sensitivity analysis demonstrated that the findings of the current meta-analysis are robust. Conclusion: Glucose level in PCF is more accurate than CEA for preoperative diagnosis of mucinous cysts. It may become a useful first-line test, particularly in small cysts with a limited volume of PCF. Larger studies are awaited to confirm glucose as the single test for mucinous cyst diagnosis.
MeSH term(s)	Carcinoembryonic Antigen/analysis ; Cyst Fluid/chemistry ; Glucose ; Humans ; Pancreatic Cyst/diagnosis ; Pancreatic Neoplasms/diagnosis ; Sensitivity and Specificity
Chemical Substances	Carcinoembryonic Antigen ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2021-04-11
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	391583-9
ISSN	1097-6779 ; 0016-5107
ISSN (online)	1097-6779
ISSN	0016-5107
DOI	10.1016/j.gie.2021.03.935
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Article ; Online: Glutathione Depletion Disrupts Redox Homeostasis in an Anoxia-Tolerant Invertebrate

Ferreira-Cravo, Marlize / Moreira, Daniel C. / Hermes-Lima, Marcelo

Antioxidants. 2023 May 31, v. 12, no. 6

2023

Abstract: ... an animal model of anoxia tolerance. To do so, the total GSH (tGSH) pool was depleted with l-buthionine-(S, R ...

Abstract	The upregulation of endogenous antioxidants is a widespread phenomenon in animals that tolerate hypoxia/anoxia for extended periods. The identity of the mobilized antioxidant is often context-dependent and differs among species, tissues, and stresses. Thus, the contribution of individual antioxidants to the adaptation to oxygen deprivation remains elusive. This study investigated the role of glutathione (GSH) in the control of redox homeostasis under the stress of anoxia and reoxygenation in Helix aspersa, an animal model of anoxia tolerance. To do so, the total GSH (tGSH) pool was depleted with l-buthionine-(S, R)-sulfoximine (BSO) before exposing snails to anoxia for 6 h. Then, the concentration of GSH, glutathione disulfide (GSSG), and oxidative stress markers (TBARS and protein carbonyl) and the activity of antioxidant enzymes (catalase, glutathione peroxidase, glutathione transferase, glutathione reductase, and glucose 6-phosphate dehydrogenase) were measured in foot muscle and hepatopancreas. BSO alone induced tGSH depletion by 59–75%, but no other changes happened in other variables, except for foot GSSG. Anoxia elicited a 110–114% increase in glutathione peroxidase in the foot; no other changes occurred during anoxia. However, GSH depletion before anoxia increased the GSSG/tGSH ratio by 84–90% in both tissues, which returned to baseline levels during reoxygenation. Our findings indicate that glutathione is required to withstand the oxidative challenge induced by hypoxia and reoxygenation in land snails.
Keywords	Helix aspersa ; animal models ; antioxidants ; catalase ; disulfides ; glucose 6-phosphate ; glutathione ; glutathione peroxidase ; glutathione transferase ; glutathione-disulfide reductase ; hepatopancreas ; homeostasis ; hypoxia ; invertebrates ; muscles ; oxidative stress
Language	English
Dates of publication	2023-0531
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12061197
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A comparative analysis of glucose and carcinoembryonic antigen in diagnosis of pancreatic mucinous cysts: a systematic review and meta-analysis.

Faias, Sandra / Cravo M, Marília / Chaves, Paula / Pereira, Luisa

Gastrointestinal endoscopy

2021

Abstract: Background/aims: Pancreatic cystic fluid (PCF) analysis is useful to distinguish between different cyst types and guide management. The aim of our study was to compare the diagnostic accuracy of glucose level with carcinoembryonic antigen (CEA) in PCF ... ...

Abstract	Background/aims: Pancreatic cystic fluid (PCF) analysis is useful to distinguish between different cyst types and guide management. The aim of our study was to compare the diagnostic accuracy of glucose level with carcinoembryonic antigen (CEA) in PCF for mucinous cyst diagnosis. Methods: We identified studies with PCF obtained by EUS before surgery, with cysts classified as mucinous and nonmucinous according to surgical specimens. A random effects model was used for quantitative meta-analysis. Pooled sensitivities, specificities, and summary receiver operating characteristic (SROC) curve analysis were conducted. Results: For CEA, we included 31 studies with 5268 patients, of which 2083 were referred for surgery and for glucose we included 5 studies with 460 patients, of which 275 were referred for surgery. Glucose performed better than CEA for mucinous cysts diagnosis (premalignant and malignant) with sensitivities of 0.91 (95% CI, 0.86-0.94) and 0.67 (95% CI, 0.65-0.70), specificities of 0.75 (95% CI, 0.68-0.82) and 0.80 (95% CI, 0.76-0.83), and areas under the ROC curve (AUC) of 0.95 and 0.79, respectively. Glucose had a higher sensitivity (91%), with uncommon false negative results, making it an excellent biomarker to exclude a mucinous cyst. Sensitivity analysis demonstrated that the findings of the current meta-analysis are robust. Conclusion: Glucose level in PCF is more accurate than CEA for preoperative diagnosis of mucinous cysts. It may become a useful first line test, particularly in small cysts with limited volume of PCF. Larger studies are awaited to confirm glucose as the single test for mucinous cyst diagnosis.
Language	English
Publishing date	2021-01-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	391583-9
ISSN	1097-6779 ; 0016-5107
ISSN (online)	1097-6779
ISSN	0016-5107
DOI	10.1016/j.gie.2021.01.021
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Article ; Online: The Importance of Self-Management in the Context of Personalized Care in COPD.

Cravo, Ana / Attar, Darush / Freeman, Daryl / Holmes, Steve / Ip, Lindsay / Singh, Sally J

International journal of chronic obstructive pulmonary disease

2022 Volume 17, Page(s) 231–243

Abstract: Despite current guidelines and decades of evidence on the benefits of a self-management approach, self-management of COPD remains relatively under-utilized in clinical care compared with other chronic diseases. However, self-management interventions can ... ...

Abstract	Despite current guidelines and decades of evidence on the benefits of a self-management approach, self-management of COPD remains relatively under-utilized in clinical care compared with other chronic diseases. However, self-management interventions can play a valuable role in supporting people with COPD to respond to changing symptoms, and thereby make appropriate decisions regarding the management of their own chronic condition. In this review, we discuss the history and evolution of the concept of self-management, assess current multidisciplinary support programs and clinical interactions designed to optimize self-management, and reflect on how effective these are in terms of clinical and humanistic outcomes. We also evaluate the mechanisms for encouraging change from protocol-based care towards a more personalized care approach, and discuss the role of digital self-management interventions and the importance of addressing health inequalities in COPD treatment, which have been accelerated by the COVID-19 pandemic. Reflecting on the importance of self-management in the context of symptom monitoring and provision of educational support, including information from patient organizations and charities, we discuss the ideal components of a self-management plan for COPD and provide six key recommendations for its implementation: 1) better education for healthcare professionals on disease management and consultation skills; 2) new targets and priorities for patient-focused outcomes; 3) skills gap audits to identify barriers to self-management; 4) best practice sharing within primary care networks and ongoing professional development; 5) enhanced initial consultations to establish optimal self-management from the outset; and 6) negotiation and sharing of self-management plans at the point of diagnosis.
MeSH term(s)	COVID-19 ; Humans ; Pandemics ; Pulmonary Disease, Chronic Obstructive/diagnosis ; Pulmonary Disease, Chronic Obstructive/therapy ; SARS-CoV-2 ; Self-Management
Language	English
Publishing date	2022-01-22
Publishing country	New Zealand
Document type	Journal Article ; Review
ZDB-ID	2212419-6
ISSN	1178-2005 ; 1176-9106
ISSN (online)	1178-2005
ISSN	1176-9106
DOI	10.2147/COPD.S343108
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Article: Functional Status Following Pulmonary Rehabilitation: Responders and Non-Responders.

Souto-Miranda, Sara / Mendes, Maria A / Cravo, João / Andrade, Lília / Spruit, Martijn A / Marques, Alda

Journal of clinical medicine

2022 Volume 11, Issue 3

Abstract: The 6 min walking test (6MWT) has been largely studied. Less is, however, known about responders and non-responders to pulmonary rehabilitation (PR) in other meaningful activities. We explored responders and non-responders and the predictors of response ... ...

Abstract	The 6 min walking test (6MWT) has been largely studied. Less is, however, known about responders and non-responders to pulmonary rehabilitation (PR) in other meaningful activities. We explored responders and non-responders and the predictors of response to PR in the 1 min sit-to-stand test (1 min STS) and the 6MWT and compared both measures in classifying responders. An observational study was conducted with 121 people with chronic obstructive pulmonary disease (COPD). The functional status was assessed before and after PR. Baseline differences between responders and non-responders were tested with Mann-Whitney U, chi-square, or Fisher exact tests. Predictors were explored with binary logistic regressions. Agreement between both measures was assessed with chi-square, Cohen's kappa, and McNemar tests. There were 54.5% and 57.0% of responders in the 1 min STS and the 6MWT, respectively. The proportion of responders was significantly different (
Language	English
Publishing date	2022-01-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm11030518
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Article: Membranolytic Mechanism of Amphiphilic Antimicrobial β-Stranded [KL]

Schweigardt, Fabian / Strandberg, Erik / Wadhwani, Parvesh / Reichert, Johannes / Bürck, Jochen / Cravo, Haroldo L P / Burger, Luisa / Ulrich, Anne S

Biomedicines

2022 Volume 10, Issue 9

Abstract: Amphipathic peptides can act as antibiotics due to membrane permeabilization. KL peptides with the repetitive sequence [Lys-Leu] ...

Abstract	Amphipathic peptides can act as antibiotics due to membrane permeabilization. KL peptides with the repetitive sequence [Lys-Leu]
Language	English
Publishing date	2022-08-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10092071
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Article ; Online: Body composition and Crohn's disease behavior: Is adiposity the main game changer?

Velho, Sónia / Morão, Bárbara / Gouveia, Catarina / Agostinho, Lisa / Torres, Joana / Maio, Rui / Baracos, Vickie E / Cravo, Marília

Nutrition (Burbank, Los Angeles County, Calif.)

2022 Volume 108, Page(s) 111959

Abstract: ... plugin.: Results: We included 63 patients: 33 (52%) men, median age 35 y. Disease location (L) and ...

Abstract	Objective: We investigated the association between body composition upon diagnosis and complicated phenotypes and time until surgery in patients with Crohn's disease (CD). Methods: We conducted a retrospective cohort study including patients with CD who had a computed tomography enterography or a magnetic resonance enterography performed ≤6 mo of diagnosis. Skeletal muscle and visceral and subcutaneous adipose tissue cross-sectional areas were determined with computed tomography or magnetic resonance images at the third lumbar vertebral level, processed with the sliceOmatic (TomoVison, Magog, QC, Canada) and ABACS plugin. Results: We included 63 patients: 33 (52%) men, median age 35 y. Disease location (L) and behavior (B) according to the Montreal classification were L1 (ileal disease) = 28 (44%), L2 (colonic disease) = 13 (21%), L3(ileocolonic disease) = 18 (28%), L1 + L4 (ileal and isolated upper disease) = 1 (2%), L3 + L4 (ileocolonic and isolated upper disease) = 3 (5%), B1 (non-stricturing) = 39 (62%), B2 (stricturing) = 11 (17%), and B3 (penetrating)= 13 (21%); 20 (32%) patients had perianal disease. Visceral obesity was present in 12 (19%) patients and was associated with higher age of CD onset (median 60 versus 34 y; P = 0.002) and complicated disease behavior (B2 and B3) (66.7% versus 31.7%; P = 0.021). After adjusting for age and perianal disease, total adipose tissue was associated with a 4% increase in the odds of complicated behavior per 10 cm Conclusion: Total fat was associated with complicated disease phenotype and visceral obesity, with higher risk of abdominal surgery and shorter time until surgery.
MeSH term(s)	Humans ; Crohn Disease/complications ; Retrospective Studies ; Adiposity ; Obesity, Abdominal/complications ; Tomography, X-Ray Computed
Language	English
Publishing date	2022-12-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639259-3
ISSN	1873-1244 ; 0899-9007
ISSN (online)	1873-1244
ISSN	0899-9007
DOI	10.1016/j.nut.2022.111959
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Article ; Online: Genetic testing

Faias, Sandra / Pereira, Luisa / Luís, Ângelo / Chaves, Paula / Cravo, Marília

World journal of gastroenterology

2019 Volume 25, Issue 26, Page(s) 3450–3467

Abstract: Background: Carcinoembryonic antigen (CEA) and cytology in pancreatic cystic fluid are suboptimal for evaluation of pancreatic cystic neoplasms. Genetic testing and microforceps biopsy are promising tools for pre-operative diagnostic improvement but ... ...

Abstract	Background: Carcinoembryonic antigen (CEA) and cytology in pancreatic cystic fluid are suboptimal for evaluation of pancreatic cystic neoplasms. Genetic testing and microforceps biopsy are promising tools for pre-operative diagnostic improvement but comparative performance of both methods is unknown. Aim: To compare the accuracy of genetic testing and microforceps biopsy in pancreatic cysts referred for surgery. Methods: We performed a literature search in Medline, Scopus, and Web of Science for studies evaluating genetic testing of cystic fluid and microforceps biopsy of pancreatic cysts, with endoscopic ultrasound with fine-needle aspiration (EUS-FNA) prior to surgery and surgical pathology as reference standard for diagnosis. We evaluated the diagnostic accuracy for: 1- benign cysts; 2- mucinous low-risk cysts; 3- high-risk cysts, and the diagnostic yield and rate of correctly identified cysts with microforceps biopsy and molecular analysis. We also assessed publication bias, heterogeneity, and study quality. Results: Eight studies, including 1206 patients, of which 203 (17%) referred for surgery who met the inclusion criteria were analyzed in the systematic review, and seven studies were included in the meta-analysis. Genetic testing and microforceps biopsies were identical for diagnosis of benign cysts. Molecular analysis was superior for diagnosis of both low and high-risk mucinous cysts, with sensitivities of 0.89 (95%CI: 0.79-0.95) and 0.57 (95%CI: 0.42-0.71), specificities of 0.88 (95%CI: 0.75-0.95) and 0.88 (95%CI: 0.80-0.93) and AUC of 0.9555 and 0.92, respectively. The diagnostic yield was higher in microforceps biopsies than in genetic analysis (0.73 Conclusion: Genetic testing and microforceps biopsies are useful second tests, with identical results in benign pancreatic cysts. Genetic analysis performs better for low- and high-risk cysts but has lower diagnostic yield.
MeSH term(s)	Cyst Fluid/cytology ; Diagnosis, Differential ; Endoscopic Ultrasound-Guided Fine Needle Aspiration/instrumentation ; Genetic Testing ; Humans ; Pancreas/pathology ; Pancreatic Cyst/diagnosis ; Pancreatic Cyst/genetics ; Pancreatic Cyst/pathology ; Pancreatic Neoplasms/diagnosis ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/pathology ; Preoperative Period ; Sensitivity and Specificity
Language	English
Publishing date	2019-09-30
Publishing country	United States
Document type	Comparative Study ; Evaluation Study ; Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v25.i26.3450
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