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Article ; Online: A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain.

Ino, Daisuke / Tanaka, Yudai / Hibino, Hiroshi / Nishiyama, Masaaki

2022 Volume 19, Issue 10, Page(s) 1286–1294

Abstract: Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of ...

Abstract	Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIA
MeSH term(s)	Animals ; Behavior, Animal/physiology ; Brain ; Ligands ; Mice ; Neuropeptides ; Oxytocin/physiology
Chemical Substances	Ligands ; Neuropeptides ; Oxytocin (50-56-6)
Language	English
Publishing date	2022-09-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/s41592-022-01597-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Classification of multiple emotional states from facial expressions in head-fixed mice using a deep learning-based image analysis.

Tanaka, Yudai / Nakata, Takuto / Hibino, Hiroshi / Nishiyama, Masaaki / Ino, Daisuke

PloS one

2023 Volume 18, Issue 7, Page(s) e0288930

Abstract: Facial expressions are widely recognized as universal indicators of underlying internal states in most species of animals, thereby presenting as a non-invasive measure for assessing physical and mental conditions. Despite the advancement of artificial ... ...

Abstract	Facial expressions are widely recognized as universal indicators of underlying internal states in most species of animals, thereby presenting as a non-invasive measure for assessing physical and mental conditions. Despite the advancement of artificial intelligence-assisted tools for automated analysis of voluminous facial expression data in human subjects, the corresponding tools for mice still remain limited so far. Considering that mice are the most prevalent model animals for studying human health and diseases, a comprehensive characterization of emotion-dependent patterns of facial expressions in mice could extend our knowledge on the basis of emotions and the related disorders. Here, we present a framework for the development of a deep learning-powered tool for classifying facial expressions in head-fixed mouse. We demonstrate that our machine vision was capable of accurately classifying three different emotional states from lateral facial images in head-fixed mouse. Moreover, we objectively determined how our classifier characterized the differences among the facial images through the use of an interpretation technique called Gradient-weighted Class Activation Mapping. Importantly, our machine vision presumably discerned the data by leveraging multiple facial features. Our approach is likely to facilitate the non-invasive decoding of a variety of emotions from facial images in head-fixed mice.
MeSH term(s)	Humans ; Animals ; Mice ; Facial Expression ; Artificial Intelligence ; Deep Learning ; Emotions/physiology ; Physical Examination
Language	English
Publishing date	2023-07-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0288930
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Fulminant Guillain-Barré syndrome secondary to

Kutsuna, Fumiya / Soeda, Momoko / Hibino, Aiko / Tokuda, Masahiro / Miura, Shiro / Iwanaga, Hiroshi

eNeurologicalSci

2023 Volume 31, Page(s) 100454

Abstract: The most common infection preceding Guillain-Barré syndrome (GBS) ... ...

Abstract	The most common infection preceding Guillain-Barré syndrome (GBS) is
Language	English
Publishing date	2023-02-25
Publishing country	Netherlands
Document type	Case Reports
ZDB-ID	2838045-9
ISSN	2405-6502 ; 2405-6502
ISSN (online)	2405-6502
ISSN	2405-6502
DOI	10.1016/j.ensci.2023.100454
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Article ; Online: Downfolding of the epiglottis into the laryngeal inlet after tracheal intubation using the McGRATH

Soma, Haruno / Furutani, Kenta / Hibino, Ayaka / Hibino, Akinobu / Baba, Hiroshi

JA clinical reports

2020 Volume 6, Issue 1, Page(s) 42

Abstract: Background: Downfolding of the epiglottis into the laryngeal inlet is considered to be a rare complication of tracheal intubation. We describe a case of epiglottic downfolding during tracheal intubation using a McGrath videolaryngoscope (McGRATH: Case ...

Abstract	Background: Downfolding of the epiglottis into the laryngeal inlet is considered to be a rare complication of tracheal intubation. We describe a case of epiglottic downfolding during tracheal intubation using a McGrath videolaryngoscope (McGRATH Case presentation: A 44-year-old female was scheduled for breast reconstruction surgery. Intubation was performed using a McGrath videolaryngoscope. After intubation, videolaryngoscopy revealed that the epiglottis was inverted and folded down into the laryngeal inlet. We elevated the larynx anteriorly using the McGrath videolaryngoscope, enabling the downfolded epiglottis to be pulled out from the laryngeal inlet and restored to its original position. After surgery, the patient was extubated without any complications. Conclusions: When using the McGrath videolaryngoscope, both glottic exposure similar to that achieved with the Macintosh laryngoscope and careful observation of the epiglottis should enable the prevention, detection, and treatment of epiglottic downfolding into the laryngeal inlet.
Language	English
Publishing date	2020-06-05
Publishing country	Germany
Document type	Journal Article
ISSN	2363-9024
ISSN (online)	2363-9024
DOI	10.1186/s40981-020-00349-0
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Article ; Online: Effect of surface treatment of CAD/CAM resin composites on the shear bond strength of self-adhesive resin cement.

Nagasawa, Yuko / Hibino, Yasushi / Eda, Yoshikazu / Nakajima, Hiroshi

Dental materials journal

2020 Volume 40, Issue 2, Page(s) 364–378

Abstract: This study examined the effects of sandblasting, hydrofluoric acid etching and priming on the shear bond strength of self-adhesive resin cement between seven different CAD/CAM resin composites and a resin composite core material at 24-h after cement ... ...

Abstract	This study examined the effects of sandblasting, hydrofluoric acid etching and priming on the shear bond strength of self-adhesive resin cement between seven different CAD/CAM resin composites and a resin composite core material at 24-h after cement mixing. Five surface treatments [control (C), sandblasting (S), priming (P), sandblasting with priming (SP), and 9% HF etching with priming (HFP)] were performed respectively for disc specimens of CAD/CAM blocks. There were no significant differences in bond strength among the C, S, and P, except for one block (p>0.05). SP showed a greater bond strength than S. Weibull moduli were not changed significantly among all treatments for all blocks, whereas the strengths with 5% and 95% failure probability of SP and HFP showed greater values than the others. The bond strengths of HFP were comparable to those of SP. Priming after sandblasting or HF etching could be effective to increase the bond strength of CAD/CAM blocks.
MeSH term(s)	Ceramics ; Composite Resins ; Dental Bonding ; Dental Cements ; Materials Testing ; Resin Cements ; Shear Strength ; Surface Properties
Chemical Substances	Composite Resins ; Dental Cements ; Resin Cements
Language	English
Publishing date	2020-10-24
Publishing country	Japan
Document type	Journal Article
ZDB-ID	605650-7
ISSN	1881-1361 ; 0287-4547
ISSN (online)	1881-1361
ISSN	0287-4547
DOI	10.4012/dmj.2019-431
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Article ; Online: A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis.

Emoto, Yuri / Katayama, Ryoya / Hibino, Emi / Ishihara, Sho / Goda, Natsuko / Tenno, Takeshi / Kobashigawa, Yoshihiro / Morioka, Hiroshi / Hiroaki, Hidekazu

Methods and protocols

2023 Volume 6, Issue 3

Abstract: The detection and quantification of protein-protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In ... ...

Abstract	The detection and quantification of protein-protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In this study, we improved the cohesive and sticky properties of gelatinized starch by supplementing it with agarose, resulting in a harder gel that could coat the bottom of a microtiter plate. The resulting gelatinized starch/agarose mixture allowed for the efficient immobilization of MBP-tagged proteins on the coated plates, enabling the use of indirect ELISA-like PPI assays. By using the enzymatic activity of GST as an indicator, we succeeded in determining the dissociation constants between MBP-tagged and GST-tagged proteins on 96-well microtiter plates and a microplate reader without any expensive specialized equipment.
Language	English
Publishing date	2023-04-22
Publishing country	Switzerland
Document type	Journal Article
ISSN	2409-9279
ISSN (online)	2409-9279
DOI	10.3390/mps6030044
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Book ; Online: Elastic Softening in Synthetic Diamonds

Yanagisawa, Tatsuya / Hibino, Ruo / Hidaka, Hiroyuki / Amitsuka, Hiroshi / Tashima, Toshiyuki / Akatsu, Mitsuhiro / Nemoto, Yuichi / Zherlitsyn, Sergei / Wosnitza, Joachim

2024

Abstract: This study reveals a novel phenomenon demonstrating the softening of synthetic diamonds when cooled to very low temperatures below 1 K. Herein, we argue that this elastic softening can be attributed to the effect of electric-quadrupole degrees of freedom ...

Abstract	This study reveals a novel phenomenon demonstrating the softening of synthetic diamonds when cooled to very low temperatures below 1 K. Herein, we argue that this elastic softening can be attributed to the effect of electric-quadrupole degrees of freedom of the dangling bonds in the neutral single-atom vacancies of carbon. We present the results of ultrasonic investigations of single-crystalline synthetic diamonds, namely type-IIa (colorless) and Ib (yellow) diamonds grown by high-pressure-high-temperature synthesis as well as type-IIa diamond grown by chemical vapor deposition. We observe a magnetic-field-insensitive softening of the elastic constant $C_{44}$ in all samples at low temperatures below 1 K. Our results strongly suggest a ppb level concentration of neutral single-atomic vacancies in all investigated diamonds. Our findings open new avenues for the quantitative determination of single neutral vacancies in non-irradiated diamonds, an important information needed for their potential application for quantum technology and next-generation semiconductor devices. Comment: 6 Pages, 3 figures with supplemental materials
Keywords	Condensed Matter - Materials Science ; Condensed Matter - Strongly Correlated Electrons ; Physics - Applied Physics
Publishing date	2024-01-06
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of storage conditions on mechanical properties of resin composite blanks for CAD/CAM crowns.

Hibino, Yasushi / Nagasawa, Yuko / Eda, Yoshikazu / Shigeta, Hirotaka / Nakajima, Hiroshi

Dental materials journal

2020 Volume 39, Issue 5, Page(s) 742–751

Abstract: The objective of this study was to examine the effects of storage conditions on the flexural strengths of resin composite materials for CAD/CAM restorations. Seven commercially available resin composite CAD/CAM blanks were examined. Rectangular specimens ...

Abstract	The objective of this study was to examine the effects of storage conditions on the flexural strengths of resin composite materials for CAD/CAM restorations. Seven commercially available resin composite CAD/CAM blanks were examined. Rectangular specimens (4.0×1.2×4.0 mm) of each material were trimmed from the blanks and subjected to thermal cycling between 5°C and 55°C in deionized water at 10,000 cycles or stored in 37°C deionized water for one week or air for one day (n=10 for each condition). The difference in storage condition between water immersion and thermal cycling did not affect the flexural strengths of resin composite materials for CAD/CAM examined in this study. The resin composite block CS300 made from Bis-MEPP and UDMA showed the greatest flexural strength under all storage conditions and less deterioration of strength by thermal cycling and water immersion among the resin composite blocks tested.
MeSH term(s)	Composite Resins ; Computer-Aided Design ; Crowns ; Flexural Strength ; Materials Testing ; Surface Properties
Chemical Substances	Composite Resins
Language	English
Publishing date	2020-05-16
Publishing country	Japan
Document type	Journal Article
ZDB-ID	605650-7
ISSN	1881-1361 ; 0287-4547
ISSN (online)	1881-1361
ISSN	0287-4547
DOI	10.4012/dmj.2019-202
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [Analysis of the mechanism underlying formation of the endocochlear potential in the inner ear].

Hibino, Hiroshi

Nihon yakurigaku zasshi. Folia pharmacologica Japonica

2009 Volume 133, Issue 5, Page(s) 247–251

MeSH term(s)	Action Potentials/physiology ; Cochlea/blood supply ; Cochlea/physiology ; Ear, Inner/physiology ; Humans ; KCNQ1 Potassium Channel/physiology ; Lymph/physiology ; Potassium/metabolism ; Potassium Channels, Inwardly Rectifying/physiology ; Potassium Channels, Voltage-Gated/physiology
Chemical Substances	KCNE1 protein, human ; KCNQ1 Potassium Channel ; Kcnj10 (channel) ; Potassium Channels, Inwardly Rectifying ; Potassium Channels, Voltage-Gated ; Potassium (RWP5GA015D)
Language	Japanese
Publishing date	2009-05-01
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	1097532-9
ISSN	1347-8397 ; 0015-5691
ISSN (online)	1347-8397
ISSN	0015-5691
DOI	10.1254/fpj.133.247
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Article ; Online: A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

Yuri Emoto / Ryoya Katayama / Emi Hibino / Sho Ishihara / Natsuko Goda / Takeshi Tenno / Yoshihiro Kobashigawa / Hiroshi Morioka / Hidekazu Hiroaki

Methods and Protocols, Vol 6, Iss 44, p

2023 Volume 44

Abstract: The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In ... ...

Abstract	The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In this study, we improved the cohesive and sticky properties of gelatinized starch by supplementing it with agarose, resulting in a harder gel that could coat the bottom of a microtiter plate. The resulting gelatinized starch/agarose mixture allowed for the efficient immobilization of MBP-tagged proteins on the coated plates, enabling the use of indirect ELISA-like PPI assays. By using the enzymatic activity of GST as an indicator, we succeeded in determining the dissociation constants between MBP-tagged and GST-tagged proteins on 96-well microtiter plates and a microplate reader without any expensive specialized equipment.
Keywords	gelatinized starch ; maltose-binding protein ; microplate-based assay ; protein–protein interaction ; dissociation constant determination ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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