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Article: The Functions of an NAC Transcription Factor, GhNAC2-A06, in Cotton Response to Drought Stress.

Saimi, Gulisitan / Wang, Ziyu / Liusui, Yunhao / Guo, Yanjun / Huang, Gengqing / Zhao, Huixin / Zhang, Jingbo

2023 Volume 12, Issue 21

Abstract: Drought stress imposes severe constraints on crop growth and yield. The NAC transcription factors (TF) play a pivotal role in regulating plant stress responses. However, the biological functions and regulatory mechanisms of many cotton NACs have not been ...

Abstract	Drought stress imposes severe constraints on crop growth and yield. The NAC transcription factors (TF) play a pivotal role in regulating plant stress responses. However, the biological functions and regulatory mechanisms of many cotton NACs have not been explored. In this study, we report the cloning and characterization of
Language	English
Publishing date	2023-11-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12213755
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Article ; Online: Phenolic Content and in Vitro Antioxidant, Anti-Inflammatory and antimicrobial Evaluation of Algerian Ruta graveolens L.

Mokhtar, Meriem / Youcefi, Fatma / Keddari, Soumia / Saimi, Yahia / Otsmane Elhaou, Siham / Cacciola, Francesco

Chemistry & biodiversity

2022 Volume 19, Issue 9, Page(s) e202200545

Abstract: Plants constitute a valuable source of natural antioxidants such as polyphenols and are responsible for exhibiting many biologically significant functions. Ruta species including Ruta chalepensis L. and Ruta graveolens L. are widespread species in ... ...

Abstract	Plants constitute a valuable source of natural antioxidants such as polyphenols and are responsible for exhibiting many biologically significant functions. Ruta species including Ruta chalepensis L. and Ruta graveolens L. are widespread species in Algeria and are used as medicinal plants to treat various diseases; however, so far, most of the conducted studies focused on analyzing alkaloids and essential oils mostly on R. chalepensis. The aim of the present research is to investigate the phenolic profile of the aerial parts of Ruta graveolens L. from Algeria and assess its in vitro antioxidant, anti-inflammatory and antimicrobial properties. The total polyphenols and flavonoids were assessed using colorimetric methods, and the individual polyphenols were identified and quantified using HPLC-DAD-ESI-MS. The antioxidant activity was evaluated with DPPH and β-carotene tests, and the anti-inflammatory activity with inhibition of bovine serum albumin denaturation and HRBC membrane stabilization methods. The results showed that Ruta graveolens extract is rich in phenolic compounds with a total phenol and flavonoid contents of 41.63±0.394 mg GAE/gE and 13.97±0.33 mg EQ/gE, respectively. Nine phenolic compounds were determined, including three phenolic acids and six flavonoids. Rutin was the major phenolic compound in Ruta graveolens (464.95 μg/g), followed by syringic acid (179.74 μg/g), and naringenin (109.78 μg/g). R. graveolens phenolic extract also showed good antioxidant activity with values of 0.77 mM TE/g DW and 0.37 mM β-CE/g DW with DPPH and β-carotene tests, respectively. For the anti-inflammatory activity, the highest tested concentration (200 μg/mL) gave 50.61 % of inhibition of the denaturation of albumin and 44.12 % of membrane stabilization. With regards to antimicrobial results, Staphylococcus aureus was the most sensitive bacteria with an inhibition zone of 14.37 mm and MIC value of 0.625 mg/mL, followed by Listeria monocytogenes (11.75 mm and MIC=1.25 mg/mL), and Escherichia coli (10.25 mm and MIC=1.25 mg/mL).
MeSH term(s)	Algeria ; Alkaloids ; Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents/pharmacology ; Anti-Inflammatory Agents/pharmacology ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Flavonoids/pharmacology ; Oils, Volatile/chemistry ; Phenols/analysis ; Phenols/pharmacology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Polyphenols ; Ruta/chemistry ; Rutin ; Serum Albumin, Bovine ; beta Carotene
Chemical Substances	Alkaloids ; Anti-Bacterial Agents ; Anti-Infective Agents ; Anti-Inflammatory Agents ; Antioxidants ; Flavonoids ; Oils, Volatile ; Phenols ; Plant Extracts ; Polyphenols ; beta Carotene (01YAE03M7J) ; Serum Albumin, Bovine (27432CM55Q) ; Rutin (5G06TVY3R7)
Language	English
Publishing date	2022-08-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2139001-0
ISSN	1612-1880 ; 1612-1872
ISSN (online)	1612-1880
ISSN	1612-1872
DOI	10.1002/cbdv.202200545
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Article ; Online: Geometric Antibody Engineering Reveals the Spatial Factor on the Efficacy of Bispecific T Cell Engagers.

Zhang, Yu / Yang, Zhe / Saimi, Dilizhatai / Shen, Xiaowen / Ye, Junqing / Yu, Bingke / Pefaur, Noah / Scheer, Justin M / Nixon, Andrew E / Chen, Zhixing

ACS chemical biology

2024 Volume 19, Issue 4, Page(s) 916–925

Abstract: Bispecific antibodies (BsAbs) represent an emerging class of biologics that can recognize two different antigens or epitopes. T-cell engagers (TcEs) bind two targets in trans on the cell surface of the effector and target cell to induce proximal immune ... ...

Abstract	Bispecific antibodies (BsAbs) represent an emerging class of biologics that can recognize two different antigens or epitopes. T-cell engagers (TcEs) bind two targets in trans on the cell surface of the effector and target cell to induce proximal immune effects, opening exciting windows for immunotherapies. To date, the engineering of BsAbs has been mainly focused on tuning the molecular weight and valency. However, the effects of spatial factors on the biological functions of BsAbs have been less explored due to the lack of biochemical methods to precisely manipulate protein geometry. Here, we studied the geometric effects of the TcEs. First, by genetically inserting rigidly designed ankyrin repeat proteins into TcEs, we revealed that the efficacy progressively decreased as the spacer distance of the two binding domains increased. Then, we constructed 26 pairs of TcEs with the same size but varying orientations using click chemistry-mediated conjugation at different mutation sites. We found that linear ligation sites play a minor role in modulating cell-killing efficacy. Next, we rendered the TcEs' advanced topology by cyclization chemistry using the SpyTag/SpyCatcher pair or sortase ligation approaches. Cyclized TcEs were generally more potent than their linear counterparts. Particularly, sortase A cyclized TcEs, bearing a minimal tagging motif, exhibited better cell-killing efficacy in vitro and improved stability both in vitro and in vivo compared to the linear TcE. This work combines modern bioconjugation chemistry and protein engineering tools for antibody engineering, shedding light on the elusive spatial factors of BsAbs functionality.
MeSH term(s)	Antibodies, Bispecific/genetics ; Antibodies, Bispecific/therapeutic use ; Antibodies, Bispecific/chemistry ; Click Chemistry ; Protein Engineering/methods ; Proteins ; T-Lymphocytes/immunology ; Humans
Chemical Substances	Antibodies, Bispecific ; Proteins
Language	English
Publishing date	2024-03-16
Publishing country	United States
Document type	Journal Article
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/acschembio.3c00728
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Article ; Online: Risk Factors of Mortality From

Wang, Yuqiong / Zhou, Xiaoyi / Saimi, Maidinuer / Huang, Xu / Sun, Ting / Fan, Guohui / Zhan, Qingyuan

Frontiers in public health

2021 Volume 9, Page(s) 680108

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Coinfection ; Cytomegalovirus Infections/epidemiology ; Humans ; Pneumocystis ; Pneumonia, Pneumocystis ; Risk Factors
Language	English
Publishing date	2021-06-16
Publishing country	Switzerland
Document type	Meta-Analysis ; Research Support, Non-U.S. Gov't ; Systematic Review
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2021.680108
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Article ; Online: γ-Glutamyltranspeptidase-Triggered Intracellular Gadolinium Nanoparticle Formation Enhances the T

Hai, Zijuan / Ni, Yanhan / Saimi, Dilizhatai / Yang, Hongyi / Tong, Haiyang / Zhong, Kai / Liang, Gaolin

Nano letters

2019 Volume 19, Issue 4, Page(s) 2428–2433

Abstract: Magnetic resonance imaging (MRI) is advantageous in the diagnosis of deep internal cancers, but contrast agents (CAs) are always needed to improve MRI sensitivity. Gadolinium (Gd)-based agents are routinely used as ... ...

Abstract	Magnetic resonance imaging (MRI) is advantageous in the diagnosis of deep internal cancers, but contrast agents (CAs) are always needed to improve MRI sensitivity. Gadolinium (Gd)-based agents are routinely used as T
MeSH term(s)	Animals ; Contrast Media/administration & dosage ; Contrast Media/chemistry ; Gadolinium/administration & dosage ; Gadolinium/chemistry ; HeLa Cells ; Humans ; Magnetic Resonance Imaging/methods ; Metal Nanoparticles/administration & dosage ; Metal Nanoparticles/chemistry ; Mice ; Neoplasms/diagnosis ; Neoplasms/diagnostic imaging ; Neoplasms/pathology ; Xenograft Model Antitumor Assays ; gamma-Glutamyltransferase/administration & dosage ; gamma-Glutamyltransferase/chemistry
Chemical Substances	Contrast Media ; Gadolinium (AU0V1LM3JT) ; gamma-Glutamyltransferase (EC 2.3.2.2)
Language	English
Publishing date	2019-03-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1530-6992
ISSN (online)	1530-6992
DOI	10.1021/acs.nanolett.8b05154
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Article ; Online: Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway.

Lv, Jiadi / Liu, Yuying / Mo, Siqi / Zhou, Yabo / Chen, Fengye / Cheng, Feiran / Li, Cong / Saimi, Dilizhatai / Liu, Mengyu / Zhang, Huafeng / Tang, Ke / Ma, Jingwei / Wang, Zhenfeng / Zhu, Qiangqiang / Tong, Wei-Min / Huang, Bo

Nature cell biology

2022 Volume 24, Issue 3, Page(s) 364–372

Abstract: Pancreatic ductal adenocarcinoma (PDAC) originates from normal pancreatic ducts where digestive juice is regularly produced. It remains unclear how PDAC can escape autodigestion by digestive enzymes. Here we show that human PDAC tumour cells use ... ...

Abstract	Pancreatic ductal adenocarcinoma (PDAC) originates from normal pancreatic ducts where digestive juice is regularly produced. It remains unclear how PDAC can escape autodigestion by digestive enzymes. Here we show that human PDAC tumour cells use gasdermin E (GSDME), a pore-forming protein, to mediate digestive resistance. GSDME facilitates the tumour cells to express mucin 1 and mucin 13, which form a barrier to prevent chymotrypsin-mediated destruction. Inoculation of GSDME
MeSH term(s)	Adenocarcinoma/genetics ; Animals ; Humans ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Mucin-1 ; Mucins ; Pancreatic Neoplasms/pathology ; Pore Forming Cytotoxic Proteins/physiology ; Tumor Microenvironment ; Y-Box-Binding Protein 1
Chemical Substances	GSDME protein, human ; Mucin-1 ; Mucins ; Pore Forming Cytotoxic Proteins ; Y-Box-Binding Protein 1 ; YBX1 protein, human
Language	English
Publishing date	2022-03-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1474722-4
ISSN	1476-4679 ; 1465-7392
ISSN (online)	1476-4679
ISSN	1465-7392
DOI	10.1038/s41556-022-00857-4
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Zs.A 5169: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 12: Show issues

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Article ; Online: Smart Dual Quenching Strategy Enhances the Detection Sensitivity of Intracellular Furin.

Hai, Zijuan / Wu, Jingjing / Saimi, Dilizhatai / Ni, Yanhan / Zhou, Rongbin / Liang, Gaolin

Analytical chemistry

2018 Volume 90, Issue 3, Page(s) 1520–1524

Abstract: Development of sensitive fluorescence "Turn-On" strategies for imaging enzyme activity in living cells is of disease-diagnostic importance but remains challenging. Herein, by employing a click condensation reaction and rational design of a single ... ...

Abstract	Development of sensitive fluorescence "Turn-On" strategies for imaging enzyme activity in living cells is of disease-diagnostic importance but remains challenging. Herein, by employing a click condensation reaction and rational design of a single quenched probe Cys(StBu)-Lys(Gly-Lys(DABCYL)-Gly-Gly-Arg-Arg-Val-Arg-Gly-FITC)-CBT (1), we developed a "smart" dual quenching strategy and applied it to detect intracellular furin activity with enhanced sensitivity. At physiological conditions, 1 was subjected to reduction-controlled condensation reaction to form 1-NPs and its fluorescence intensity further dropped to 1/2.8 of its original. Upon furin cleavage in vitro, the dual quenched 1-NPs had fluorescence "Turn-On" contrast 11-fold more than that of single quenched control probe FITC-Gly-Arg-Val-Arg-Arg-Gly-Gly-Lys(DABCYL)-Gly-OH (1-P). Live cell imaging results indicated that 1 showed fluorescence "Turn-On" contrast 6.3-fold of that of 1-P for sensing intracellular furin activity. We envision that, by replacing the RVRR substrate with other enzyme-cleavable ones, our versatile "smart" dual quenching strategy could be easily adjusted for the detection (or imaging) of other intracellular enzymes' activity with enhanced sensitivity.
Language	English
Publishing date	2018-01-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.7b05251
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Zs.A 4033: Show issues

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Article ; Online: Cytonuclear Estrogen Receptor Alpha Regulates Proliferation and Migration of Endometrial Carcinoma Cells.

Saimi, Mierxiati / Moriya, Shota / Li, Zhong-Lian / Miyaso, Hidenobu / Nagahori, Kenta / Kawata, Shinichi / Omotehara, Takuya / Ogawa, Yuki / Hino, Hirotsugu / Miyazawa, Keisuke / Sakabe, Kou / Itoh, Masahiro

The Tokai journal of experimental and clinical medicine

2021 Volume 46, Issue 1, Page(s) 7–16

Abstract: Objective: The effects of estrogen on cells are mediated by the estrogen receptor α (ERα) which localizes at the peri-membrane, cytoplasm, and the nucleus of cells. Therefore, we intended to investigate how cytonuclear ERα plays its roles in different ... ...

Abstract	Objective: The effects of estrogen on cells are mediated by the estrogen receptor α (ERα) which localizes at the peri-membrane, cytoplasm, and the nucleus of cells. Therefore, we intended to investigate how cytonuclear ERα plays its roles in different cellular activities. Methods: We used amino acid substituted ERα that localized at the cytoplasm and nucleus but has no direct DNA-binding activities. ERα-negative endometrial carcinoma cells (ERα-) were stably transfected with plasmid of human ERα carrying a substituted phenylalanine at position 445 with alanine (ERα-F445A). Treated with 17β-estrogen (E2) or bazedoxifene (BDF), cell proliferation, migration, and expression of kinases related to ERα signal transduction pathways were observed. Results: E2 (40 nM) significantly activated proliferation in ERα-F445A cells, but not in ERα- cells. Similarly, E2 significantly activated cell migration in ERα-F445A cells, rather than that in ERα- cells. While no obvious change in the amount of the non-phosphorylated mammalian target of Rapamycin (mTOR), the expression of mTOR phosphorylated at serine 2448 decreased, which was recovered in presence of 17β-estrogen (E2) in the ERα-F445A cells. On the other hand, the expression of focal adhesion kinase (FAK) phosphorylated at tyrosine at 297 was attenuated in the ERα-F445A cells treated with E2. Conclusion: It is suggested that the cytonuclear ERα-F445A induces phosphorylation of kinases in downstream pathways, which regulate cell proliferation and migration.
MeSH term(s)	Cell Line, Tumor ; Cell Movement/drug effects ; Cell Movement/genetics ; Cell Nucleus/metabolism ; Cell Proliferation/drug effects ; Cell Proliferation/genetics ; Cytoplasm/metabolism ; Endometrial Neoplasms/genetics ; Endometrial Neoplasms/metabolism ; Endometrial Neoplasms/pathology ; Estradiol/metabolism ; Estradiol/pharmacology ; Estrogen Receptor alpha/metabolism ; Estrogen Receptor alpha/physiology ; Female ; Focal Adhesion Protein-Tyrosine Kinases/metabolism ; Humans ; Phosphorylation ; TOR Serine-Threonine Kinases/metabolism
Chemical Substances	ESR1 protein, human ; Estrogen Receptor alpha ; Estradiol (4TI98Z838E) ; MTOR protein, human (EC 2.7.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; Focal Adhesion Protein-Tyrosine Kinases (EC 2.7.10.2)
Language	English
Publishing date	2021-04-20
Publishing country	Japan
Document type	Journal Article
ZDB-ID	431622-8
ISSN	2185-2243 ; 0385-0005
ISSN (online)	2185-2243
ISSN	0385-0005
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Zs.B 2001: Show issues

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Article ; Online: Effects of phosphorylated estrogen receptor alpha on apoptosis in human endometrial epithelial cells.

Uchida, Shunsuke / Saimi, Mierxiati / Li, Zhong-Lian / Miyaso, Hidenobu / Nagahori, Kenta / Kawata, Shinichi / Omotehara, Takuya / Ogawa, Yuki / Itoh, Masahiro

Anatomical science international

2019 Volume 95, Issue 2, Page(s) 240–250

Abstract: It is known that the activities of estrogen receptor α (ERα) can be modulated by epidermal growth factor (EGF) through the phosphatidylinostitol 3-kinase-alpha serine/threonine protein kinase (PI3K-AKT) pathway by phosphorylation. To clarify how ERα ... ...

Abstract	It is known that the activities of estrogen receptor α (ERα) can be modulated by epidermal growth factor (EGF) through the phosphatidylinostitol 3-kinase-alpha serine/threonine protein kinase (PI3K-AKT) pathway by phosphorylation. To clarify how ERα functions are regulated in endometrial cells during menstrual cycle, molecules related to phosphorylation of ERα (pERα) were examined. It was found that the expression of phosphorylated AKT on serine 473 (pAKT-Ser473) was increased during the proliferative phase, but decreased in the secretory phase. Although the expression of pAKT on threonine 308 in the proliferative phase was only identified in the wall of arterioles, it was strongly expressed in the cytoplasm of endometrial glandular cells after entering the secretory phase. Further observations revealed that while the expression of pERα-Ser104 was constant, pERα-Ser118 was expressed following a cyclic pattern similar to that of the pAKT-Ser473. Following treatment with specific inhibitors for EGFR-PI3K-AKT pathway, it was found that while the expression of pERα-Ser118 and pERα-Ser167 was inhibited, the induced apoptosis could be antagonized by the addition of estrogen, indicating that a mitochondrial pathway is involved. Therefore, pAKT and pERα or ERα could act cooperatively on coiled arterioles and endometrial cells in order to control menstrual cycle.
MeSH term(s)	Apoptosis/genetics ; Endometrium/cytology ; Epidermal Growth Factor ; Epithelial Cells/pathology ; Estrogen Receptor alpha/physiology ; Female ; Humans ; Menstrual Cycle/metabolism ; Phosphatidylinositol 3-Kinase/metabolism ; Phosphorylation ; Proto-Oncogene Proteins c-akt/metabolism ; Signal Transduction
Chemical Substances	ESR1 protein, human ; Estrogen Receptor alpha ; Epidermal Growth Factor (62229-50-9) ; Phosphatidylinositol 3-Kinase (EC 2.7.1.137) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2019-12-10
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2079994-9
ISSN	1447-073X ; 1447-6959 ; 0022-7722
ISSN (online)	1447-073X
ISSN	1447-6959 ; 0022-7722
DOI	10.1007/s12565-019-00515-0
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Zs.A 219: Show issues

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Article ; Online: The core domain as the force sensor of the yeast mechanosensitive TRP channel.

Su, Zhenwei / Anishkin, Andriy / Kung, Ching / Saimi, Yoshiro

The Journal of general physiology

2011 Volume 138, Issue 6, Page(s) 627–640

Abstract: Stretch-activated conductances are commonly encountered in careful electric recordings. Those of known proteins (TRP, MscL, MscS, K(2p), Kv, etc.) all share a core, which houses the ion pathway and the gate, but no recognizable force-sensing domain. Like ...

Abstract	Stretch-activated conductances are commonly encountered in careful electric recordings. Those of known proteins (TRP, MscL, MscS, K(2p), Kv, etc.) all share a core, which houses the ion pathway and the gate, but no recognizable force-sensing domain. Like animal TRPs, the yeast TRPY1 is polymodal, activated by stretch force, Ca(2+), etc. To test whether its S5-S6 core senses the stretch force, we tried to uncouple it from the peripheral domains by strategic peptide insertions to block the covalent core-periphery interactions. Insertion of long unstructured peptides should distort, if not disrupt, protein structures that transmit force. Such insertions between S6 and the C-terminal tail largely removed Ca(2+) activation, showing their effectiveness. However, such insertions as well as those between S5 and the N-terminal region, which includes S1-S4, did not significantly alter mechanosensitivity. Even insertions at both locations flanking the S5-S6 core did not much alter mechanosensitivity. Tryptophan scanning mutations in S5 were also constructed to perturb possible noncovalent core-periphery contacts. The testable tryptophan mutations also have little or no effects on mechanosensitivity. Boltzmann fits of the wild-type force-response curves agree with a structural homology model for a stretch-induced core expansion of ~2 nm(2) upon opening. We hypothesize that membrane tension pulls on S5-S6, expanding the core and opening the TRPY1 gate. The core being the major force sensor offers the simplest, though not the only, explanation of why so many channels of disparate designs are mechanically sensitive. Compared with the bacterial MscL, TRPY1 is much less sensitive to force, befitting a polymodal channel that relies on multiple stimuli.
MeSH term(s)	Calcium/metabolism ; Calcium Channels/chemistry ; Calcium Channels/metabolism ; Ion Channel Gating/physiology ; Mechanotransduction, Cellular/physiology ; Mutagenesis, Site-Directed ; Protein Conformation ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/metabolism ; TRPC Cation Channels ; Transient Receptor Potential Channels/chemistry ; Transient Receptor Potential Channels/metabolism ; Tryptophan/genetics
Chemical Substances	Calcium Channels ; Saccharomyces cerevisiae Proteins ; TRPC Cation Channels ; Transient Receptor Potential Channels ; Yvc1 protein, S cerevisiae ; Tryptophan (8DUH1N11BX) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2011-11-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	3118-5
ISSN	1540-7748 ; 0022-1295
ISSN (online)	1540-7748
ISSN	0022-1295
DOI	10.1085/jgp.201110693
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