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Article: Two Candidate

Kumar, Anil / Fitoussi, Nathalia / Sanadhya, Payal / Sichov, Natalia / Bucki, Patricia / Bornstein, Menachem / Belausuv, Eduard / Brown Miyara, Sigal

Molecular plant-microbe interactions : MPMI

2023 Volume 36, Issue 2, Page(s) 79–94

Abstract: During parasitism, root-knot ... ...

Abstract	During parasitism, root-knot nematode
MeSH term(s)	Animals ; Tylenchoidea/physiology ; Parasites/genetics ; Apoptosis ; Gene Expression Profiling ; Cell Nucleus/metabolism ; Plant Roots/parasitology ; Plant Diseases
Language	English
Publishing date	2023-01-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	743331-1
ISSN	1943-7706 ; 0894-0282
ISSN (online)	1943-7706
ISSN	0894-0282
DOI	10.1094/MPMI-10-22-0212-R
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Article ; Online: Molecular cloning and characterization of high-affinity potassium transporter (AlHKT2;1) gene promoter from halophyte Aeluropus lagopoides.

Dave, Ankita / Sanadhya, Payal / Joshi, Priyanka S / Agarwal, Parinita / Agarwal, Pradeep K

International journal of biological macromolecules

2021 Volume 181, Page(s) 1254–1264

Abstract: HKT subfamily II functions as ... ...

Abstract	HKT subfamily II functions as Na
MeSH term(s)	Cation Transport Proteins/genetics ; Cloning, Molecular ; Gene Expression Regulation, Plant/genetics ; Plant Proteins/genetics ; Plants, Genetically Modified/genetics ; Promoter Regions, Genetic/genetics ; Salt-Tolerant Plants/genetics ; Seedlings/genetics ; Sodium Chloride/metabolism ; Stress, Physiological/genetics ; Symporters/genetics
Chemical Substances	Cation Transport Proteins ; HKT1 protein, plant ; Plant Proteins ; Symporters ; Sodium Chloride (451W47IQ8X)
Language	English
Publishing date	2021-05-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2021.05.038
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Article ; Online: Two Candidate Meloidogyne javanica Effector Genes, MjShKT and MjPUT3: A Functional Investigation of Their Roles in Regulating Nematode Parasitism

Anil Kumar / Fitoussi, Nathalia / Sanadhya, Payal / Sichov, Natalia / Bucki, Patricia / Bornstein, Menachem / Belausuv, Eduard / Brown Miyara, Sigal

Molecular Plant-Microbe Interactions. 2023 Feb., v. 36, no. 2 p.79-94

2023

Abstract: During parasitism, root-knot nematode Meloidogyne spp. inject molecules termed effectors that have multifunctional roles in construction and maintenance of nematode feeding sites. As an outcome of transcriptomic analysis of Meloidogyne javanica, we ... ...

Abstract	During parasitism, root-knot nematode Meloidogyne spp. inject molecules termed effectors that have multifunctional roles in construction and maintenance of nematode feeding sites. As an outcome of transcriptomic analysis of Meloidogyne javanica, we identified and characterized two differentially expressed genes encoding the predicted proteins MjShKT, carrying a Stichodactyla toxin (ShKT) domain, and MjPUT3, carrying a ground-like domain, both expressed during nematode parasitism of the tomato plant. Fluorescence in-situ hybridization revealed expression of MjShKT and MjPUT3 in the dorsal esophageal glands, suggesting their injection into host cells. MjShKT expression was upregulated during the parasitic life stages, to a maximum at the mature female stage, whereas MjPUT3 expression increased in third- to fourth-stage juveniles. Subcellular in-planta localization of MjShKT and MjPUT3 using a fused fluorescence marker indicated MjShKT co-occurrence with the endoplasmic reticulum, the perinuclear endoplasmatic reticulum, and the Golgi organelle markers, while MjPUT3 localized, to some extent, within the endoplasmatic reticulum and was clearly observed within the nucleoplasm. MjShKT inhibited programmed cell death induced by overexpression of MAPKKKα and Gpa2/RBP-1. Overexpression of MjShKT in tomato hairy roots allowed an increase in nematode reproduction, as indicated by the high number of eggs produced on roots overexpressing MjShKT. Roots overexpressing MjPUT3 were characterized by enhanced root growth, with no effect on nematode development on those roots. Investigation of the two candidate effectors suggested that MjShKT is mainly involved in manipulating the plant effector-triggered immune response toward establishment and maintenance of active feeding sites, whereas MjPUT3 might modulate roots morphology in favor of nematode fitness in the host roots. Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Keywords	Meloidogyne javanica ; endoplasmic reticulum ; females ; fluorescence ; fluorescence in situ hybridization ; gene expression regulation ; immune response ; parasitism ; programmed cell death ; reproduction ; root growth ; root-knot nematodes ; roots ; tomatoes ; toxins ; transcriptomics ; effector-triggered immunity ; effectors ; feeding sites ; plant host immunity ; root knot nematodes ; suppression of plant defense
Language	English
Dates of publication	2023-02
Size	p. 79-94.
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	743331-1
ISSN	1943-7706 ; 0894-0282
ISSN (online)	1943-7706
ISSN	0894-0282
DOI	10.1094/MPMI-10-22-0212-R
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Article: Tomato Divinyl Ether-Biosynthesis Pathway Is Implicated in Modulating of Root-Knot Nematode

Sanadhya, Payal / Kumar, Anil / Bucki, Patricia / Fitoussi, Nathalia / Carmeli-Weissberg, Mira / Borenstein, Menachem / Brown-Miyara, Sigal

Frontiers in plant science

2021 Volume 12, Page(s) 670772

Abstract: The role of the 9-lipoxygenase (9-LOX)-derived oxylipins in plant defense is mainly known in solanaceous plants. In this work, we identify the functional role of the tomato divinyl ether synthase (LeDES) branch, which exclusively converts 9- ... ...

Abstract	The role of the 9-lipoxygenase (9-LOX)-derived oxylipins in plant defense is mainly known in solanaceous plants. In this work, we identify the functional role of the tomato divinyl ether synthase (LeDES) branch, which exclusively converts 9-hydroperoxides to the 9-divinyl ethers (DVEs) colneleic acid (CA) and colnelenic acid (CnA), during infection by the root-knot nematode
Language	English
Publishing date	2021-08-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2021.670772
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Article: Ion homeostasis in a salt-secreting halophytic grass.

Sanadhya, Payal / Agarwal, Parinita / Agarwal, Pradeep K

AoB PLANTS

2015 Volume 7

Abstract: Salinity adversely affects plant growth and development, and disturbs intracellular ion homeostasis, resulting in cellular toxicity. Plants that tolerate salinity, halophytes, do so by manifesting numerous physiological and biochemical processes in ... ...

Abstract	Salinity adversely affects plant growth and development, and disturbs intracellular ion homeostasis, resulting in cellular toxicity. Plants that tolerate salinity, halophytes, do so by manifesting numerous physiological and biochemical processes in coordination to alleviate cellular ionic imbalance. The present study was undertaken to analyse the salt tolerance mechanism in Aeluropus lagopoides (L.) trin. Ex Thw. (Poaceae) at both physiological and molecular levels. Plants secreted salt from glands, which eventually produced pristine salt crystals on leaves and leaf sheaths. The rate of salt secretion increased with increasing salt concentration in the growth medium. Osmotic adjustment was mainly achieved by inorganic osmolytes (Na(+)) and at 100 mM NaCl no change was observed in organic osmolytes in comparison to control plants. At 300 mM NaCl and with 150 mM NaCl + 150 mM KCl, the concentration of proline, soluble sugars and amino acids was significantly increased. Transcript profiling of transporter genes revealed differential spatial and temporal expressions in both shoot and root tissues in a manner synchronized towards maintaining ion homeostasis. In shoots, AlHKT2;1 transcript up-regulation was observed at 12 and 24 h in all the treatments, whereas in roots, maximum induction was observed at 48 h with K(+) starvation. The HAK transcript was relatively abundant in shoot tissue with all the treatments. The plasma membrane Na(+)/H(+) antiporter, SOS1, and tonoplast Na(+)/H(+) antiporter, NHX1, were found to be significantly up-regulated in shoot tissue. Our data demonstrate that AlHKT2;1, HAK, SOS1, NHX1 and V-ATPase genes play a pivotal role in regulating the ion homeostasis in A. lagopoides.
Language	English
Publishing date	2015-05-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2555823-7
ISSN	2041-2851
ISSN	2041-2851
DOI	10.1093/aobpla/plv055
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Article ; Online: Caenorhabditis elegans susceptibility to Daldinia cf. concentrica bioactive volatiles is coupled with expression activation of the stress-response transcription factor daf-16, a part of distinct nematicidal action.

Sanadhya, Payal / Bucki, Patricia / Liarzi, Orna / Ezra, David / Gamliel, Abraham / Braun Miyara, Sigal

PloS one

2018 Volume 13, Issue 5, Page(s) e0196870

Abstract: The bionematicidal effect of a synthetic volatile mixture (SVM) of four volatile organic compounds (VOCs) emitted by the endophytic fungus Daldinia cf. concentrica against the devastating plant-parasitic root-knot nematode Meloidogyne javanica has been ... ...

Abstract	The bionematicidal effect of a synthetic volatile mixture (SVM) of four volatile organic compounds (VOCs) emitted by the endophytic fungus Daldinia cf. concentrica against the devastating plant-parasitic root-knot nematode Meloidogyne javanica has been recently demonstrated in both in vitro and greenhouse experiments. However, the mode of action governing the observed irreversible paralysis of J2 larvae upon exposure to SVM is unknown. To unravel the mechanism underlying the anthelmintic and nematicidal activities, we used the tractable model worm Caenorhabditis elegans. C. elegans was also susceptible to both the fungal VOCs and SVM. Among compounds comprising SVM, 3-methyl-1-butanol, (±)-2-methyl-1-butanol, and 4-heptanone showed significant nematicidal activity toward L1, L4 and young adult stages. Egg hatching was only negatively affected by 4-heptanone. To determine the mechanism underlying this activity, we examined the response of C. elegans mutants for glutamate-gated chloride channel and acetylcholine transporter, targets of the nematicidal drugs ivermectin and aldicarb, respectively, to 4-heptanone and SVM. These aldicarb- and ivermectin-resistant mutants retained susceptibility upon exposure to 4-heptanone and SVM. Next, we used C. elegans TJ356 strain zIs356 (daf-16::GFP+rol-6), LD1 ldIs7 [skn-1B/C::GFP + pRF4(rol-6(su1006))], LD1171 ldIs3 [gcs-1p::gfp; rol-6(su1006))], CL2166 dvIs19 (gst-4p::GFP) and CF1553 muIs84 (sod-3p::GFP+rol-6), which have mutations in genes regulating multiple stress responses. Following exposure of L4 larvae to 4-heptanone or SVM, there was clear nuclear translocation of DAF-16::GFP, and SKN-1::GFP indicating that their susceptibility involves DAF-16 and SKN1 regulation. Application of 4-heptanone, but not SVM, induced increased expression of, gcs-1::GFP and gst-4::GFP compared to controls. In contrast, application of 4-heptanone or SVM to the sod-3::GFP line elicited a significant decline in overall fluorescence intensity compared to controls, indicating SOD-3 downregulation and therefore overall reduction in cellular redox machinery. Our data indicate that the mode of action of SVM and 4-heptanone from D. cf. concentrica differs from that of currently available nematicides, potentially offering new solutions for nematode management.
MeSH term(s)	Aldicarb/pharmacology ; Animals ; Anthelmintics/isolation & purification ; Anthelmintics/pharmacology ; Caenorhabditis elegans/drug effects ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/growth & development ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/agonists ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Forkhead Transcription Factors/agonists ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/metabolism ; Gene Expression Regulation ; Ivermectin/pharmacology ; Ketones/chemistry ; Ketones/pharmacology ; Larva/drug effects ; Larva/genetics ; Larva/growth & development ; Larva/metabolism ; Pentanols/chemistry ; Pentanols/pharmacology ; Superoxide Dismutase/genetics ; Superoxide Dismutase/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Volatile Organic Compounds/isolation & purification ; Volatile Organic Compounds/pharmacology ; Xylariales/chemistry ; Xylariales/metabolism ; Zygote/drug effects ; Zygote/growth & development ; Zygote/metabolism
Chemical Substances	2-methylbutanol ; Anthelmintics ; Caenorhabditis elegans Proteins ; DNA-Binding Proteins ; Forkhead Transcription Factors ; Ketones ; Pentanols ; Transcription Factors ; Volatile Organic Compounds ; daf-16 protein, C elegans ; skn-1 protein, C elegans (148733-36-2) ; Ivermectin (70288-86-7) ; Aldicarb (8V071SH05P) ; 4-heptanone (9BN582JQ61) ; isopentyl alcohol (DEM9NIT1J4) ; Sod-3 protein, C elegans (EC 1.15.1.1) ; Superoxide Dismutase (EC 1.15.1.1)
Language	English
Publishing date	2018-05-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0196870
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A Low-Affinity K+ Transporter AlHKT2;1 from Recretohalophyte Aeluropus lagopoides Confers Salt Tolerance in Yeast.

Sanadhya, Payal / Agarwal, Parinita / Khedia, Jackson / Agarwal, Pradeep K

Molecular biotechnology

2015 Volume 57, Issue 6, Page(s) 489–498

Abstract: The high-affinity potassium transporters (HKT) are highly important for stress tolerance in plants as they uniquely maintain K(+)/Na(+) ratio for their survival and growth. In this study a novel HKT gene AlHKT2;1 was isolated and characterized from salt ... ...

Abstract	The high-affinity potassium transporters (HKT) are highly important for stress tolerance in plants as they uniquely maintain K(+)/Na(+) ratio for their survival and growth. In this study a novel HKT gene AlHKT2;1 was isolated and characterized from salt secreting halophyte, Aeluropus lagopoides. The AlHKT2;1 cDNA comprised of an open reading frame of 1,581 bp, encoding a protein of 526 amino acid residues. It belongs to class II HKTs and showed high homology with other HKT genes. Functional characterization of AlHKT2;1 in both K(+) uptake-deficient (WΔ6) and Na(+)-sensitive yeast mutants (G19) showed the characteristic feature of low-affinity K(+) transporter supporting the growth at >1 mM KCl concentration. The transformed yeast cells showed high sensitivity to NaCl; however, the addition of KCl along with NaCl support the growth of AlHKT2;1 expressing mutant. Ion content analysis of yeast cells with AlHKT2;1 grown in high NaCl medium supplemented with KCl revealed that salt tolerance was correlated with accumulation of K(+) during salt stress. These results suggest that AlHKT2;1 plays an important role in the K(+) uptake during salt stress and in maintaining a high K(+)/Na(+) ratio in the cytosol.
MeSH term(s)	Amino Acid Sequence ; Carrier Proteins/chemistry ; Carrier Proteins/genetics ; Carrier Proteins/physiology ; Genetic Complementation Test ; Molecular Sequence Data ; Phylogeny ; Poaceae/genetics ; Potassium/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/physiology ; Sequence Homology, Amino Acid ; Sodium Chloride
Chemical Substances	Carrier Proteins ; Sodium Chloride (451W47IQ8X) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2015-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1193057-3
ISSN	1559-0305 ; 1073-6085
ISSN (online)	1559-0305
ISSN	1073-6085
DOI	10.1007/s12033-015-9842-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Caenorhabditis elegans susceptibility to Daldinia cf. concentrica bioactive volatiles is coupled with expression activation of the stress-response transcription factor daf-16, a part of distinct nematicidal action.

Payal Sanadhya / Patricia Bucki / Orna Liarzi / David Ezra / Abraham Gamliel / Sigal Braun Miyara

PLoS ONE, Vol 13, Iss 5, p e

2018 Volume 0196870

Abstract	The bionematicidal effect of a synthetic volatile mixture (SVM) of four volatile organic compounds (VOCs) emitted by the endophytic fungus Daldinia cf. concentrica against the devastating plant-parasitic root-knot nematode Meloidogyne javanica has been recently demonstrated in both in vitro and greenhouse experiments. However, the mode of action governing the observed irreversible paralysis of J2 larvae upon exposure to SVM is unknown. To unravel the mechanism underlying the anthelmintic and nematicidal activities, we used the tractable model worm Caenorhabditis elegans. C. elegans was also susceptible to both the fungal VOCs and SVM. Among compounds comprising SVM, 3-methyl-1-butanol, (±)-2-methyl-1-butanol, and 4-heptanone showed significant nematicidal activity toward L1, L4 and young adult stages. Egg hatching was only negatively affected by 4-heptanone. To determine the mechanism underlying this activity, we examined the response of C. elegans mutants for glutamate-gated chloride channel and acetylcholine transporter, targets of the nematicidal drugs ivermectin and aldicarb, respectively, to 4-heptanone and SVM. These aldicarb- and ivermectin-resistant mutants retained susceptibility upon exposure to 4-heptanone and SVM. Next, we used C. elegans TJ356 strain zIs356 (daf-16::GFP+rol-6), LD1 ldIs7 [skn-1B/C::GFP + pRF4(rol-6(su1006))], LD1171 ldIs3 [gcs-1p::gfp; rol-6(su1006))], CL2166 dvIs19 (gst-4p::GFP) and CF1553 muIs84 (sod-3p::GFP+rol-6), which have mutations in genes regulating multiple stress responses. Following exposure of L4 larvae to 4-heptanone or SVM, there was clear nuclear translocation of DAF-16::GFP, and SKN-1::GFP indicating that their susceptibility involves DAF-16 and SKN1 regulation. Application of 4-heptanone, but not SVM, induced increased expression of, gcs-1::GFP and gst-4::GFP compared to controls. In contrast, application of 4-heptanone or SVM to the sod-3::GFP line elicited a significant decline in overall fluorescence intensity compared to controls, indicating SOD-3 ...
Keywords	Medicine ; R ; Science ; Q
Subject code	571
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Tight regulation of allene oxide synthase (AOS) and allene oxide cyclase-3 (AOC3) promote Arabidopsis susceptibility to the root-knot nematode Meloidogyne javanica

Naor, Noga / Bucki, Patricia / Gurung, Fatta B / Miyara, Sigal B / Ozalvo, Rachel / Sanadhya, Payal

European journal of plant pathology. 2018 Jan., v. 150, no. 1

2018

Abstract: Biosynthesis of the oxylipin jasmonic acid (JA) in Arabidopsis thaliana is catalyzed by a single allene oxide synthase (AOS)-encoding gene and four genes encoding four functional allene oxide cyclase (AOC) polypeptides (AOC1, AOC2, AOC3, and AOC4). To ... ...

Abstract	Biosynthesis of the oxylipin jasmonic acid (JA) in Arabidopsis thaliana is catalyzed by a single allene oxide synthase (AOS)-encoding gene and four genes encoding four functional allene oxide cyclase (AOC) polypeptides (AOC1, AOC2, AOC3, and AOC4). To elucidate the biological activities of the JA pathway in regulating the plant defense response to plant-parasitic nematodes, transgenic lines carrying the GUS reporter gene under the control of individual AOC or AOS promoters were examined. Upon penetration by second-stage juveniles (J2 s), promoter activities of AOC1, AOC3 and AOC4 appeared in the root tip and root-elongation zone, with AOC3 demonstrating highest induction. At 5 days AOC3 activity continued to be highly pronounced in the stele and root cortex, associated with nematode invasion throughout gall initiation and maturation. AOS expression appeared 3 days postinfection and accompanied all later infection stages. Mutant lines were analyzed: disruption in AOS rendered plants more resistant to nematode infection, as reflected by the decreased number of females produced on this line; loss-of-function of AOC3 rendered plants more susceptible to nematode infection. Oxylipins derived from the 9- and 13-lipoxygenase pathways were assayed for their direct inhibitory activity toward M. javanica J2 s. Clear nematicidal activity of the bioactive 9- and 13-hydroperoxides was observed. Oxylipins produced by divinyl ether synthase, colneleic acid, colnelenic acid and ω5(Z)-etherolenic acid demonstrated strong inhibitory activity. These data, along with those of other assayed oxylipins, suggest that temporal and spatial fine tuning of the JA route allowing nematodes parasitism on plant host.
Keywords	allene ; allene-oxide cyclase ; Arabidopsis thaliana ; biosynthesis ; cortex ; females ; genetically modified organisms ; hydroperoxide dehydratase ; jasmonic acid ; juveniles ; loss-of-function mutation ; Meloidogyne javanica ; mutants ; nematicidal properties ; nematode infections ; oxylipins ; parasitism ; polypeptides ; reporter genes ; root-knot nematodes ; stele
Language	English
Dates of publication	2018-01
Size	p. 149-165.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1196520-4
ISSN	0929-1873
ISSN	0929-1873
DOI	10.1007/s10658-017-1261-2
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparative molecular analysis of chemolithoautotrophic bacterial diversity and community structure from coastal saline soils, Gujarat, India.

Yousuf, Basit / Sanadhya, Payal / Keshri, Jitendra / Jha, Bhavanath

BMC microbiology

2012 Volume 12, Page(s) 150

Abstract: Background: Soils harbour high diversity of obligate as well as facultative chemolithoautotrophic bacteria that contribute significantly to CO2 dynamics in soil. In this study, we used culture dependent and independent methods to assess the community ... ...

Abstract	Background: Soils harbour high diversity of obligate as well as facultative chemolithoautotrophic bacteria that contribute significantly to CO2 dynamics in soil. In this study, we used culture dependent and independent methods to assess the community structure and diversity of chemolithoautotrophs in agricultural and coastal barren saline soils (low and high salinity). We studied the composition and distribution of chemolithoautotrophs by means of functional marker gene cbbL encoding large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and a phylogenetic marker 16S rRNA gene. The cbbL form IA and IC genes associated with carbon fixation were analyzed to gain insight into metabolic potential of chemolithoautotrophs in three soil types of coastal ecosystems which had a very different salt load and sulphur content. Results: In cbbL libraries, the cbbL form IA was retrieved only from high saline soil whereas form IC was found in all three soil types. The form IC cbbL was also amplified from bacterial isolates obtained from all soil types. A number of novel monophyletic lineages affiliated with form IA and IC phylogenetic trees were found. These were distantly related to the known cbbL sequences from agroecosystem, volcanic ashes and marine environments. In 16S rRNA clone libraries, the agricultural soil was dominated by chemolithoautotrophs (Betaproteobacteria) whereas photoautotrophic Chloroflexi and sulphide oxidizers dominated saline ecosystems. Environmental specificity was apparently visible at both higher taxonomic levels (phylum) and lower taxonomic levels (genus and species). The differentiation in community structure and diversity in three soil ecosystems was supported by LIBSHUFF (P = 0.001) and UniFrac. Conclusion: This study may provide fundamentally new insights into the role of chemolithoautotrophic and photoautotrophic bacterial diversity in biochemical carbon cycling in barren saline soils. The bacterial communities varied greatly among the three sites, probably because of differences in salinity, carbon and sulphur contents. The cbbL form IA-containing sulphide-oxidizing chemolithotrophs were found only in high saline soil clone library, thus giving the indication of sulphide availability in this soil ecosystem. This is the first comparative study of the community structure and diversity of chemolithoautotrophic bacteria in coastal agricultural and saline barren soils using functional (cbbL) and phylogenetic (16S rDNA) marker genes.
MeSH term(s)	Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Bacteria/metabolism ; Biota ; Chemoautotrophic Growth ; Cluster Analysis ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; India ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Ribulose-Bisphosphate Carboxylase/genetics ; Sequence Analysis, DNA ; Soil Microbiology
Chemical Substances	DNA, Bacterial ; DNA, Ribosomal ; RNA, Ribosomal, 16S ; Ribulose-Bisphosphate Carboxylase (EC 4.1.1.39)
Language	English
Publishing date	2012-07-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1471-2180
ISSN (online)	1471-2180
DOI	10.1186/1471-2180-12-150
Database	MEDical Literature Analysis and Retrieval System OnLINE

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