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  1. AU=Edwards Adrianne N.
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  1. Artikel: The RgaS-RgaR two-component system promotes

    Edwards, Adrianne N / McBride, Shonna M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The ability to form a dormant spore is essential for the survival of the anaerobic, gastrointestinal ... ...

    Abstract The ability to form a dormant spore is essential for the survival of the anaerobic, gastrointestinal pathogen
    Sprache Englisch
    Erscheinungsdatum 2023-06-27
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.06.26.546640
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: The predicted acetoin dehydrogenase pathway represses sporulation of

    Wetzel, Daniela / Rizvi, Arshad / Edwards, Adrianne N / McBride, Shonna M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Clostridioides difficile: Importance: Clostridioides difficile, ...

    Abstract Clostridioides difficile
    Importance: Clostridioides difficile,
    Sprache Englisch
    Erscheinungsdatum 2023-07-29
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.07.28.551048
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system.

    Edwards, Adrianne N / McBride, Shonna M

    PLoS genetics

    2023  Band 19, Heft 10, Seite(n) e1010841

    Abstract: The ability to form a dormant spore is essential for the survival of the anaerobic pathogen, Clostridioides difficile, outside of the mammalian gastrointestinal tract. The initiation of sporulation is governed by the master regulator of sporulation, ... ...

    Abstract The ability to form a dormant spore is essential for the survival of the anaerobic pathogen, Clostridioides difficile, outside of the mammalian gastrointestinal tract. The initiation of sporulation is governed by the master regulator of sporulation, Spo0A, which is activated by phosphorylation. Multiple sporulation factors control Spo0A phosphorylation; however, this regulatory pathway is not well defined in C. difficile. We discovered that RgaS and RgaR, a conserved orphan histidine kinase and orphan response regulator, function together as a cognate two-component regulatory system to directly activate transcription of several genes. One of these targets, agrB1D1, encodes gene products that synthesize and export a small quorum-sensing peptide, AgrD1, which positively influences expression of early sporulation genes. Another target, a small regulatory RNA now known as SpoZ, impacts later stages of sporulation through a small hypothetical protein and an additional, unknown regulatory mechanism(s). Unlike Agr systems in many organisms, AgrD1 does not activate the RgaS-RgaR two-component system, and thus, is not responsible for autoregulating its own production. Altogether, we demonstrate that C. difficile utilizes a conserved two-component system that is uncoupled from quorum-sensing to promote sporulation through two distinct regulatory pathways.
    Mesh-Begriff(e) Animals ; Clostridioides difficile/physiology ; Clostridioides/metabolism ; Histidine Kinase/genetics ; Histidine Kinase/metabolism ; Phosphorylation ; Quorum Sensing/genetics ; Spores, Bacterial/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Mammals/metabolism
    Chemische Substanzen Histidine Kinase (EC 2.7.13.1) ; Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-10-16
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1010841
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: The impact of orphan histidine kinases and phosphotransfer proteins on the regulation of clostridial sporulation initiation.

    Mehdizadeh Gohari, Iman / Edwards, Adrianne N / McBride, Shonna M / McClane, Bruce A

    mBio

    2024  Band 15, Heft 4, Seite(n) e0224823

    Abstract: Sporulation is an important feature of the clostridial life cycle, facilitating survival of these bacteria in harsh environments, contributing to disease transmission for pathogenic species, and sharing common early steps that are also involved in ... ...

    Abstract Sporulation is an important feature of the clostridial life cycle, facilitating survival of these bacteria in harsh environments, contributing to disease transmission for pathogenic species, and sharing common early steps that are also involved in regulating industrially important solvent production by some non-pathogenic species. Initial genomics studies suggested that Clostridia lack the classical phosphorelay that phosphorylates Spo0A and initiates sporulation in
    Mesh-Begriff(e) Histidine Kinase/genetics ; Histidine Kinase/metabolism ; Histidine/metabolism ; Phosphorylation ; Transcription Factors/metabolism ; Bacillus/metabolism ; Clostridium/genetics ; Clostridium/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Spores, Bacterial/metabolism ; Bacillus subtilis/genetics ; Gene Expression Regulation, Bacterial
    Chemische Substanzen Histidine Kinase (EC 2.7.13.1) ; Histidine (4QD397987E) ; Transcription Factors ; Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2024-03-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.02248-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: The pH-responsive SmrR-SmrT system modulates

    Wetzel, Daniela / Carter, Zavier A / Monteiro, Marcos P / Edwards, Adrianne N / McBride, Shonna M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Clostridioides difficile: Importance: C. ... ...

    Abstract Clostridioides difficile
    Importance: C. difficile
    Sprache Englisch
    Erscheinungsdatum 2023-11-03
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.11.02.565354
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: The pH-responsive SmrR-SmrT system modulates

    Wetzel, Daniela / Carter, Zavier A / Monteiro, Marcos P / Edwards, Adrianne N / Scharer, Christopher D / McBride, Shonna M

    Infection and immunity

    2024  Band 92, Heft 3, Seite(n) e0046123

    Abstract: Clostridioides ... ...

    Abstract Clostridioides difficile
    Mesh-Begriff(e) Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Clostridioides difficile ; Spores, Bacterial ; Gene Expression Regulation, Bacterial ; Drug Resistance, Bacterial ; Hydrogen-Ion Concentration ; Bacterial Proteins/metabolism ; Mammals
    Chemische Substanzen Anti-Bacterial Agents ; Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2024-02-12
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00461-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel: A Conserved Switch Controls Virulence, Sporulation, and Motility in

    DiCandia, Michael A / Edwards, Adrianne N / Lee, Cheyenne D / Monteiro, Marcos P / Cuebas, Germ N Vargas / Bagchi, Pritha / McBride, Shonna M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Spore formation is required for environmental survival and transmission of the human ... ...

    Abstract Spore formation is required for environmental survival and transmission of the human enteropathogenic
    Sprache Englisch
    Erscheinungsdatum 2023-03-28
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.03.28.534590
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Three Orphan Histidine Kinases Inhibit Clostridioides difficile Sporulation.

    Edwards, Adrianne N / Wetzel, Daniela / DiCandia, Michael A / McBride, Shonna M

    Journal of bacteriology

    2022  Band 204, Heft 5, Seite(n) e0010622

    Abstract: The ability of the anaerobic gastrointestinal pathogen Clostridioides difficile to survive outside the host relies on the formation of dormant endospores. Spore formation is contingent on the activation of a conserved transcription factor, Spo0A, by ... ...

    Abstract The ability of the anaerobic gastrointestinal pathogen Clostridioides difficile to survive outside the host relies on the formation of dormant endospores. Spore formation is contingent on the activation of a conserved transcription factor, Spo0A, by phosphorylation. Multiple kinases and phosphatases regulate Spo0A activity in other spore-forming organisms; however, these factors are not well conserved in C. difficile. Previously, we discovered that deletion of a predicted histidine kinase, CD1492, increases sporulation, indicating that CD1492 inhibits C. difficile spore formation. In this study, we investigate the functions of additional predicted orphan histidine kinases CD2492, CD1579, and CD1949, which are hypothesized to regulate Spo0A phosphorylation. Disruption of CD2492 also increased sporulation frequency, similarly to the
    Mesh-Begriff(e) Bacillus subtilis/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Clostridioides ; Clostridioides difficile/genetics ; Gene Expression Regulation, Bacterial ; Histidine/metabolism ; Histidine Kinase/genetics ; Histidine Kinase/metabolism ; Phosphoric Monoester Hydrolases/metabolism ; Spores, Bacterial/metabolism
    Chemische Substanzen Bacterial Proteins ; Histidine (4QD397987E) ; Histidine Kinase (EC 2.7.13.1) ; Phosphoric Monoester Hydrolases (EC 3.1.3.2)
    Sprache Englisch
    Erscheinungsdatum 2022-04-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00106-22
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel: Determination of the

    Edwards, Adrianne N / McBride, Shonna M

    Bio-protocol

    2017  Band 7, Heft 3

    Abstract: The anaerobic, gastrointestinal pathogen, ...

    Abstract The anaerobic, gastrointestinal pathogen,
    Sprache Englisch
    Erscheinungsdatum 2017-05-11
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.2125
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Glycine fermentation by

    Rizvi, Arshad / Vargas-Cuebas, Germán / Edwards, Adrianne N / DiCandia, Michael A / Carter, Zavier A / Lee, Cheyenne D / Monteiro, Marcos P / McBride, Shonna M

    Infection and immunity

    2023  Band 91, Heft 10, Seite(n) e0031923

    Abstract: Clostridioides ... ...

    Abstract Clostridioides difficile
    Mesh-Begriff(e) Cricetinae ; Animals ; Clostridioides difficile/metabolism ; Cathelicidins ; Fermentation ; Virulence ; Amino Acids/metabolism ; Glycine/metabolism ; Bacterial Proteins/genetics ; Spores/metabolism
    Chemische Substanzen Cathelicidins ; Amino Acids ; Glycine (TE7660XO1C) ; Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-09-27
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00319-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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