LIVIVO - Search results -

Search results

Result 1 - 10 of total 4266

Search options

Article: 3D brown adipogenesis to create âBrown-Fat-in-Microstrandsâ

Unser, Andrea M / Bridget Mooney / David T. Corr / Yubing Xie / Yu-Hua Tseng

Biomaterials. 2016 Jan., v. 75

2016

Abstract: ... of pluripotent stem cells, and brown preadipocytes as a positive control. Brown adipocyte differentiation ... in-Microstrandsâ from pluripotent stem cells opens up a new arena to understanding brown adipogenesis and ... of brown adipocytes in energy balance and treating obesity. To address this need, we created 3D âBrown-Fat ...

Abstract	The ability of brown adipocytes (fat cells) to dissipate energy as heat shows great promise for the treatment of obesity and other metabolic disorders. Employing pluripotent stem cells, with an emphasis on directed differentiation, may overcome many issues currently associated with primary fat cell cultures. In addition, three-dimensional (3D) cell culture systems are needed to better understand the role of brown adipocytes in energy balance and treating obesity. To address this need, we created 3D âBrown-Fat-in-Microstrandsâ by microfluidic synthesis of alginate hydrogel microstrands that encapsulated cells and directly induced cell differentiation into brown adipocytes, using mouse embryonic stem cells (ESCs) as a model of pluripotent stem cells, and brown preadipocytes as a positive control. Brown adipocyte differentiation within microstrands was confirmed by immunocytochemistry and qPCR analysis of the expression of the brown adipocyte-defining marker uncoupling protein 1 (UCP1), as well as other general adipocyte markers. Cells within microstrands were responsive to a Î²-adrenergic agonist with an increase in gene expression of thermogenic UCP1, indicating that these âBrown-Fat-in-Microstrandsâ are functional. The ability to create âBrown-Fat-in-Microstrandsâ from pluripotent stem cells opens up a new arena to understanding brown adipogenesis and its implications in obesity and metabolic disorders.
Keywords	adipocytes ; adipogenesis ; agonists ; beta-adrenergic agonists ; cell culture ; embryonic stem cells ; encapsulation ; energy balance ; gene expression ; heat ; hydrocolloids ; immunocytochemistry ; metabolic diseases ; mice ; models ; obesity ; quantitative polymerase chain reaction
Language	English
Dates of publication	2016-01
Size	p. 123-134.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	603079-8
ISSN	0142-9612
ISSN	0142-9612
DOI	10.1016/j.biomaterials.2015.10.017
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.B 2371: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Protein detection by fluorescence of manually cleaned high-speed dental handpieces.

Souza, Fábio B / Bruna, Camila Q M / Mesiano, Rosa A B / Cicarelli, Regina M B / Perrett, David / Graziano, Kazuko U

Infection control and hospital epidemiology

2022 Volume 44, Issue 5, Page(s) 840–842

Abstract: We analyzed the effectiveness of manual cleaning protocols performed on high-speed dental handpieces, using protein identification by fluorescence. Although one protocol was able to able to lower the amount of protein, >40% of the handpieces showed ... ...

Abstract	We analyzed the effectiveness of manual cleaning protocols performed on high-speed dental handpieces, using protein identification by fluorescence. Although one protocol was able to able to lower the amount of protein, >40% of the handpieces showed amounts of residual protein at unacceptable levels.
MeSH term(s)	Fluorescence ; Dental High-Speed Equipment ; Proteins/analysis ; Equipment Contamination
Chemical Substances	Proteins
Language	English
Publishing date	2022-04-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639378-0
ISSN	1559-6834 ; 0195-9417 ; 0899-823X
ISSN (online)	1559-6834
ISSN	0195-9417 ; 0899-823X
DOI	10.1017/ice.2022.36
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1714: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Advantages of using biologically generated

Jaber, Malak A / de Falco, Bruna / Abdelrazig, Salah / Ortori, Catharine A / Barrett, David A / Kim, Dong-Hyun

Analytical methods : advancing methods and applications

2023 Volume 15, Issue 24, Page(s) 2925–2934

Abstract: In comprehensive lipidomics studies, accurate quantification is essential but biological and/or clinical relevance is often hindered due to unwanted variations such as lipid degradation during sample preparation, matrix effects and non-linear responses ... ...

Abstract	In comprehensive lipidomics studies, accurate quantification is essential but biological and/or clinical relevance is often hindered due to unwanted variations such as lipid degradation during sample preparation, matrix effects and non-linear responses of analytical instruments. In addition, the wide chemical diversity of lipids can complicate the accurate identification of individual lipids. These analytical limitations can potentially be corrected efficiently by the use of lipid-specific isotopically labelled internal standards (IS) but currently such IS mixtures have limited coverage of the mammalian lipidome. In this study, an
MeSH term(s)	Animals ; Chromatography, Liquid/methods ; Lipidomics ; Saccharomyces cerevisiae ; Escherichia coli ; Tandem Mass Spectrometry/methods ; Lipids/analysis ; Lipids/chemistry ; Carbon Isotopes/chemistry ; Mammals
Chemical Substances	Lipids ; Carbon Isotopes
Language	English
Publishing date	2023-06-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d3ay00460k
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Reply by Authors.

Pino, Bruna / Song, Jonathan / Orlando, Andrew / Wang, David

Urology practice

2024 Volume 11, Issue 3, Page(s) 568

Language	English
Publishing date	2024-03-14
Publishing country	United States
Document type	Journal Article
ISSN	2352-0787
ISSN (online)	2352-0787
DOI	10.1097/UPJ.0000000000000532
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Erratum to: A genetic approach reveals different modes of action of prefoldins.

Blanco-Touriñán, Noel / Esteve-Bruna, David / Serrano-Mislata, Antonio / Esquinas-Ariza, Rosa María / Resentini, Francesca / Forment, Javier / Carrasco-López, Cristian / Novella-Rausell, Claudio / Palacios-Abella, Alberto / Carrasco, Pedro / Salinas, Julio / Blázquez, Miguel Á / Alabadí, David

Plant physiology

2021 Volume 187, Issue 4, Page(s) 2879

Language	English
Publishing date	2021-10-06
Publishing country	United States
Document type	Published Erratum
ZDB-ID	208914-2
ISSN	1532-2548 ; 0032-0889
ISSN (online)	1532-2548
ISSN	0032-0889
DOI	10.1093/plphys/kiab416
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 1193: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Book ; Online: Vertically resolved aerosol variability at the Amazon Tall Tower Observatory under wet season conditions

Franco, Marco A. / Valiati, Rafael / Holanda, Bruna A. / Meller, Bruno B. / Kremper, Leslie A. / Rizzo, Luciana V. / Carbone, Samara / Morais, Fernando G. / Nascimento, Janaína P. / Andreae, Meinrat O. / Cecchini, Micael A. / Machado, Luiz A. T. / Ponczek, Milena / Pöschl, Ulrich / Walter, David / Pöhlker, Christopher / Artaxo, Paulo

eISSN:

2024

Abstract: The wet season atmosphere in the central Amazon resembles natural conditions with minimal anthropogenic influence, making it one of the rare pre-industrial-like continental areas worldwide. Previous long-term studies have analyzed the properties and ... ...

Abstract	The wet season atmosphere in the central Amazon resembles natural conditions with minimal anthropogenic influence, making it one of the rare pre-industrial-like continental areas worldwide. Previous long-term studies have analyzed the properties and sources of the natural Amazonian background aerosol. However, the vertical profile of the planetary boundary layer (PBL) has not been assessed systematically. Since 2017, such a profile assessment has been possible with the 325 m high tower at the Amazon Tall Tower Observatory (ATTO), located in a largely untouched primary forest in central Amazonia. This study investigates the variability of submicrometer aerosol concentration, size distribution, and optical properties at 60 and 325 m height in the Amazon PBL. The results show significant differences in aerosol volumes and scattering coefficients in the vertical gradient. The aerosol population was well-mixed throughout the boundary layer during the daytime but became separated upon stratification during nighttime. We also found a significant difference in the spectral dependence of the scattering coefficients between the two heights. The analysis of rainfall and related downdrafts revealed changes in the aerosol populations before and after rain events, with absorption and scattering coefficients decreasing as optically active particles are removed by wet deposition. The recovery of absorption and scattering coefficients is faster at 325 m than at 60 m. Convective events were concomitant with rapid increases in the concentration of sub-50 nm particles, likely associated with downdrafts. We found that the aerosol population near the canopy had a significantly higher mass scattering efficiency than at 325 m. It was also observed a clear spectral dependence, with values for λ = 450, 525 and 635 nm of 7.74±0.12, 5.49±0.11and 4.15±0.11 m 2 g −1 , respectively, at 60 m, while at 325 m, the values were 5.26±0.06, 3.76±0.05 and 2.46±0.04 m 2 g −1 , respectively. The equivalent aerosol refractive index results, which were ...
Subject code	333
Language	English
Publishing date	2024-01-09
Publishing country	de
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Associations of per- and polyfluoroalkyl substances, polychlorinated biphenyls, organochlorine pesticides, and polybrominated diphenyl ethers with oxidative stress markers: A systematic review and meta-analysis.

Chen, Jiawen Carmen / Baumert, Brittney O / Li, Yijie / Li, Yiping / Pan, Shudi / Robinson, Shante / Rubbo, Bruna / Costello, Elizabeth / He, Jie / Hampson, Hailey / Beglarian, Emily / Rock, Sarah / Goodrich, Jesse A / Eckel, Sandrah P / Aung, Max T / McConnell, Rob / Conti, David V / Chatzi, Lida

Environmental research

2023 Volume 239, Issue Pt 1, Page(s) 117308

Abstract: Background: Per- and polyfluoroalkyl substances (PFAS), polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and polybrominated diphenyl ethers (PBDEs) are intentionally produced persistent organic pollutants (POPs) that are resistant to ... ...

Abstract	Background: Per- and polyfluoroalkyl substances (PFAS), polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and polybrominated diphenyl ethers (PBDEs) are intentionally produced persistent organic pollutants (POPs) that are resistant to environmental degradation. Previous in-vitro and in-vivo studies have shown that POPs can induce oxidative stress, which is linked to neurodegenerative diseases, cardiovascular diseases, and cancer. However, findings in epidemiological studies are inconsistent and an evidence synthesis study is lacking to summarize the existing literature and explore research gaps. Objective: We evaluated the effects of PFAS, PCBs, OCPs, and PBDEs, on oxidative stress biomarkers in epidemiological studies. Methods: A literature search was conducted in PubMed, Embase, and Cochrane CENTRAL to identify all published studies related to POPs and oxidative stress up to December 7 Results: We identified 33 studies on OCPs, 35 on PCBs, 49 on PFAS, and 12 on PBDEs. Meta-analyses revealed significant positive associations of α-HCH with protein carbonyls (0.035 [0.017, 0.054]) and of 4'4-DDE with malondialdehyde (0.121 [0.056, 0.187]), as well as a significant negative association between 2'4-DDE and total antioxidant capacity (TAC) (-0.042 [-0.079, -0.004]), all β [95%CI]. Sign tests showed a significant positive association between PCBs and malondialdehyde (p Conclusions: Higher levels of OCPs were associated with increased levels of oxidative stress through increased pro-oxidant biomarkers involving protein oxidation, DNA damage, and lipid peroxidation, as well as decreased TAC. These findings have the potential to reveal the underlying mechanisms of POPs toxicity.
MeSH term(s)	Humans ; Antioxidants ; Biomarkers ; Environmental Pollutants/toxicity ; Fluorocarbons/toxicity ; Halogenated Diphenyl Ethers/toxicity ; Hydrocarbons, Chlorinated/toxicity ; Malondialdehyde ; Oxidative Stress ; Pesticides/toxicity ; Polychlorinated Biphenyls/toxicity
Chemical Substances	Antioxidants ; Biomarkers ; Environmental Pollutants ; Fluorocarbons ; Halogenated Diphenyl Ethers ; Hydrocarbons, Chlorinated ; Malondialdehyde (4Y8F71G49Q) ; Pesticides ; Polychlorinated Biphenyls (DFC2HB4I0K)
Language	English
Publishing date	2023-10-07
Publishing country	Netherlands
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2023.117308
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 726: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Long distance pollen dispersal and intrapopulation genetic structure determined by clonal diversity in Hymenaea stigonocarpa populations of the Brazilian savanna

Moreno, Maria A. / Boshier, David / Tarazi, Roberto / Tambarussi, Evandro V. / Aguiar, Bruna I. / Ferraz, Elza M. / Kageyama, Paulo Y. / Sebbenn, Alexandre M.

Trees. 2022 Feb., v. 36, no. 1

2022

Abstract: KEY MESSAGE: Although all populations show extensive pollen immigration, the occurrence of spatial genetic structure and biparental inbreeding decreased genetic diversity and effective population size. The Brazilian savanna is the second largest ... ...

Abstract	KEY MESSAGE: Although all populations show extensive pollen immigration, the occurrence of spatial genetic structure and biparental inbreeding decreased genetic diversity and effective population size. The Brazilian savanna is the second largest Neotropical biome, and a globally important biodiversity hotspot. Basic knowledge of the ecology and genetics of its species can help conserve this important biome. We investigated genetic diversity, spatial genetic structure (SGS), pollen dispersal, and mating system in three Hymenaea stigonocarpa populations (AS, PE, IT) in the Brazilian savanna, using microsatellite loci and samples of adult trees from all populations and seeds from the IT population. As a result of the long geographic distance between populations, the genetic differentiation among them was high (0.397). Individuals of the IT population presented a grouped distribution due to root propagation, resulting in low genotypic richness ([Formula: see text] = 0.194) and allelic richness ([Formula: see text] = 4), and high SGS ([Formula: see text] = 0.064) compared to AS and PE ([Formula: see text] > 0.98, R > 5, [Formula: see text] < 0.026) populations. The IT population showed high pollen immigration (46.4%), pollen dispersal distance (up to 3.57 km), and outcrossing rate (0.934–1.0), but matings were correlated (0.01–1), and some occurred among relatives (up to 0.098), resulting in some inbred seeds (0.140), a lower variance effective population size ([Formula: see text] = 3.02) than expected with random mating, and an estimate of 50 seed-trees required to retain an [Formula: see text] of 150 in samples of maternal progeny. The estimated minimum viable area to retain a reference ([Formula: see text]) of 1000 indicates that the current AS and IT population areas are insufficient to maintain viable populations in the long-term, demonstrating the importance of maintaining surrounding areas for conservation of these H. stigonocarpa populations.
Keywords	Hymenaea stigonocarpa ; Neotropics ; adults ; biodiversity ; ecosystems ; effective population size ; genetic structure ; genetic variation ; microsatellite repeats ; outcrossing ; pollen flow ; progeny ; savannas ; seed trees ; variance
Language	English
Dates of publication	2022-02
Size	p. 471-484.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	90595-1
ISSN	1432-2285 ; 0931-1890
ISSN (online)	1432-2285
ISSN	0931-1890
DOI	10.1007/s00468-021-02222-8
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4635: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Fostering Diversity in Urology: Addressing Ethnic Disparities in Applicant and Resident Recruitment.

Pino, Bruna / Song, Jonathan / Orlando, Andrew / Wang, David S

Urology practice

2024 Volume 11, Issue 3, Page(s) 559–566

Abstract: Introduction: In this retrospective database review, the objective was to investigate the ethnic composition of urology applicants and residents in recent years and assess whether any advancements have been made in enhancing the recruitment of ... ...

Abstract	Introduction: In this retrospective database review, the objective was to investigate the ethnic composition of urology applicants and residents in recent years and assess whether any advancements have been made in enhancing the recruitment of candidates from historically underrepresented groups in medicine. Methods: A retrospective database review was conducted on self-reported data on the ethnicity of urology applicants from academic year 2016 to 2017 (AY2016) to AY2021 and urology residents from AY2011 to AY2021. Applicant data were collected from the Association of American Medical Colleges, and resident data were collected from the Accreditation Council for Graduate Medical Education. The ethnic proportions of applicants and residents within cohorts were analyzed using χ Results: There was a statistically significant decrease in the proportion of White applicants from 61.4% to 50.5% from AY2016 to AY2021 and a statistically significant increase in the proportion of applicants of multiple race/ethnicity from 4.7% to 12.0% from AY2016 to AY2021. There were disproportionately more Hispanic/Latino residents than applicants and disproportionately fewer residents of multiple race/ethnicity than applicants in the 2 cycles analyzed. There were disproportionately fewer Black residents than applicants only in the comparison of AY2016 to AY2020 applicants to AY2020 residents. Conclusions: There continues to be a lack of ethnic representation among applicants and residents in urology from underrepresented groups in medicine, despite some measurable improvement over the years. This deficit highlights the important need for new and ongoing efforts to diversify the field.
MeSH term(s)	Humans ; Hispanic or Latino ; Urology ; Internship and Residency ; Retrospective Studies ; Black or African American
Language	English
Publishing date	2024-03-11
Publishing country	United States
Document type	Journal Article
ISSN	2352-0787
ISSN (online)	2352-0787
DOI	10.1097/UPJ.0000000000000521
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Retrospective Cohort Study of COVID-19 in Patients of the Brazilian Public Health System with SARS-CoV-2 Omicron Variant Infection.

Murari, Thiago B / Fonseca, Larissa Moraes Dos Santos / Pereira, Hernane B de B / Nascimento Filho, Aloísio S / Saba, Hugo / Scorza, Fulvio A / G de Almeida, Antônio-Carlos / Maciel, Ethel L N / Mendes, José F F / Rocha Filho, Tarcísio M / David, John R / Badaró, Roberto / Machado, Bruna Aparecida Souza / Moret, Marcelo A

Vaccines

2022 Volume 10, Issue 9

Abstract: Several vaccines against COVID-19 are now available, based on different techniques and made by different laboratories spread around the world. With the roll out of the vaccination process in an advanced stage in many countries, the reduced risk of ... ...

Abstract	Several vaccines against COVID-19 are now available, based on different techniques and made by different laboratories spread around the world. With the roll out of the vaccination process in an advanced stage in many countries, the reduced risk of hospitalization due to the Omicron variant relative to the Delta variant infection, despite the higher transmission risk of Omicron, may lead to a misinterpretation of the results, as infection by Omicron is associated with a significant reduction in severe outcomes and shorter hospitalization time than the Delta variant. We compared the in-hospital mortality due to the Omicron (Jan-Mar 2022) with Gamma (Jan 2021) and Delta (Oct-Dec 2021) variants of patients in the Brazilian public health system. This study also discusses the decrease in booster vaccine effectiveness in patients hospitalized due to the Omicron variant compared with the Delta variant. Without a remodeling of vaccines for new variants, booster doses may be necessary with a shorter time interval.
Language	English
Publishing date	2022-09-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10091504
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito