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  1. Article ; Online: Factors determining whether diffuse large B-cell lymphoma samples are detected by flow cytometry.

    Peng, David / Kodituwakku, Aruna / Le, Steven / Smith, Sandy A B C / Qiu, Min R / Earls, Peter / Field, Andrew S / Parker, Andrew J C / Law, Matthew / Milliken, Samuel T / Sewell, William A

    International journal of laboratory hematology

    2023  Volume 45, Issue 6, Page(s) 927–934

    Abstract: ... In samples not detected by flow cytometry, lymphocytes were a lower percentage of total events (p = 0.02 ... and T cells were a higher percentage of total lymphocytes (p = 0.01). Cases with high MYC protein ... expression on immunohistochemistry were less likely to be missed by FCM (p = 0.011). Detection of DLBCL was ...

    Abstract Introduction: Flow cytometry (FCM) is widely used in the diagnosis of mature B-cell neoplasms (MBN), and FCM data are usually consistent with morphological findings. However, diffuse large B-cell lymphoma (DLBCL), a common MBN, is sometimes not detected by FCM. This study aimed to explore factors that increase the likelihood of failure to detect DLBCL by FCM.
    Methods: Cases with a final diagnosis of DLBCL that were analysed by eight-colour FCM were retrospectively collated. Clinical, FCM, histopathological and genetic data were compared between cases detected and cases not detected by FCM.
    Results: DLBCL cases from 135 different patients were analysed, of which 22 (16%) were not detected by FCM. In samples not detected by flow cytometry, lymphocytes were a lower percentage of total events (p = 0.02), and T cells were a higher percentage of total lymphocytes (p = 0.01). Cases with high MYC protein expression on immunohistochemistry were less likely to be missed by FCM (p = 0.011). Detection of DLBCL was not different between germinal centre B-cell (GCB) and non-GCB subtypes, not significantly affected by the presence of necrosis or fibrosis, and not significantly different between biopsy specimens compared to fine-needle aspirates, or between samples from nodal compared to extranodal tissue.
    Conclusion: The study identifies several factors which affect the likelihood of DLBCL being missed by FCM. Even with eight-colour analysis, FCM fails to detect numerous cases of DLBCL.
    MeSH term(s) Humans ; Retrospective Studies ; Flow Cytometry ; Lymphoma, Large B-Cell, Diffuse/pathology ; B-Lymphocytes/pathology ; Germinal Center/metabolism ; Germinal Center/pathology ; Prognosis
    Language English
    Publishing date 2023-08-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.14158
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1499: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: The utility of CD200 expression and modified Matutes score in the diagnostic differentiation of mantle cell lymphoma and chronic lymphocytic leukemia using flow cytometry.

    Myles, Nicholas / Giri, Pratyush / Chim, Ivana / Kodituwakku, Aruna

    Leukemia & lymphoma

    2019  Volume 62, Issue 2, Page(s) 504–506

    MeSH term(s) Adult ; Biomarkers, Tumor ; Cell Differentiation ; Diagnosis, Differential ; Flow Cytometry ; Humans ; Immunophenotyping ; Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; Lymphoma, Mantle-Cell/diagnosis ; Lymphoma, Mantle-Cell/genetics
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2019-09-27
    Publishing country United States
    Document type Letter
    ZDB-ID 1042374-6
    ISSN 1029-2403 ; 1042-8194
    ISSN (online) 1029-2403
    ISSN 1042-8194
    DOI 10.1080/10428194.2019.1663420
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2997: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article: Generation of murine monoclonal antibodies to Haemophilus influenzae type b capsular polysaccharide by in vivo immunization.

    Kodituwakku, Aruna P / Zola, Heddy / Roberton, Don M

    Hybridoma and hybridomics

    2004  Volume 23, Issue 3, Page(s) 160–167

    Abstract: Haemophilus influenzae type b (Hib) is a pathogenic gram-negative bacterium associated with human disease, especially in young children. Protective immune response to Hib results from antibodies developed against the polyribosylribitol phosphate (PRP) ... ...

    Abstract Haemophilus influenzae type b (Hib) is a pathogenic gram-negative bacterium associated with human disease, especially in young children. Protective immune response to Hib results from antibodies developed against the polyribosylribitol phosphate (PRP) capsular polysaccharide of the bacterium. Several investigators in the study of immune response to Hib have produced human monoclonal antibodies to PRP. Only two previous groups have reported the generation of murine anti-PRP monoclonal antibodies using either immunizing mice with inactivated Hib bacteria or a combination of in vivo and in vitro immunization of mice with PRP conjugate antigen (PRP-D). In this present study, we generated murine anti-PRP monoclonal antibody secreting hybridomas for the first time by simple in vivo immunization with PRP conjugate antigen (PRP-T). The anti-PRP antibodies from one hybridoma clone (B10) are further characterized and potential applications are discussed.
    MeSH term(s) Agglutination Tests ; Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/isolation & purification ; Bacterial Capsules ; Enzyme-Linked Immunosorbent Assay ; Haemophilus Vaccines/immunology ; Haemophilus influenzae type b/immunology ; Hybridomas/immunology ; Immunization ; Immunoglobulin Isotypes/immunology ; Luminescent Measurements ; Mice ; Microbial Sensitivity Tests ; Polysaccharides, Bacterial/immunology
    Chemical Substances Antibodies, Monoclonal ; Haemophilus Vaccines ; Haemophilus influenzae type b polysaccharide vaccine ; Immunoglobulin Isotypes ; Polysaccharides, Bacterial
    Language English
    Publishing date 2004-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2079028-4
    ISSN 1536-8599
    ISSN 1536-8599
    DOI 10.1089/1536859041224325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1876: Show issues Location:
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  4. Article: Isolation of antigen-specific B cells.

    Kodituwakku, Aruna P / Jessup, Claire / Zola, Heddy / Roberton, Don M

    Immunology and cell biology

    2003  Volume 81, Issue 3, Page(s) 163–170

    Abstract: Cell separation techniques are important in immunology. Major cell populations can be separated successfully with high purity. However, isolation of cells which are specific for particular antigens is more challenging because of the relatively small ... ...

    Abstract Cell separation techniques are important in immunology. Major cell populations can be separated successfully with high purity. However, isolation of cells which are specific for particular antigens is more challenging because of the relatively small numbers of antigen-specific cells, and the lack of independent markers available to determine the purity of the isolated population. In this review, the literature describing three principal techniques used to separate antigen-specific cells has been reviewed. Particular emphasis has been placed on yield and purity; the two most important parameters of any purification method. The most promising isolation methods have used immunomagnetic sorting and multiparametric flow cytometric analysis.
    MeSH term(s) Animals ; B-Lymphocytes/immunology ; Epitopes/immunology ; Flow Cytometry/methods ; Humans ; Immunomagnetic Separation/methods ; Rosette Formation/methods
    Chemical Substances Epitopes
    Language English
    Publishing date 2003-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 284057-1
    ISSN 1440-1711 ; 0818-9641
    ISSN (online) 1440-1711
    ISSN 0818-9641
    DOI 10.1046/j.1440-1711.2003.01152.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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