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  1. Article ; Online: Association of State COVID-19 Vaccination Prioritization With Vaccination Rates Among Incarcerated Persons.

    Biondi, Breanne E / Leifheit, Kathryn M / Mitchell, Carmen R / Skinner, Alexandra / Brinkley-Rubinstein, Lauren / Raifman, Julia

    JAMA network open

    2022  Volume 5, Issue 4, Page(s) e226960

    MeSH term(s) COVID-19/epidemiology ; COVID-19/prevention & control ; COVID-19 Vaccines ; Humans ; Prisoners ; Prisons ; Vaccination
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2022-04-01
    Publishing country United States
    Document type Journal Article
    ISSN 2574-3805
    ISSN (online) 2574-3805
    DOI 10.1001/jamanetworkopen.2022.6960
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.

    Tchoukalova, Yourka D / Zacharias, Stephanie R C / Mitchell, Natalie / Madsen, Cathy / Myers, Cheryl E / Gadalla, Dina / Skinner, Jessica / Kopaczka, Katarzyna / Gramignoli, Roberto / Lott, David G

    Stem cell research & therapy

    2022  Volume 13, Issue 1, Page(s) 31

    Abstract: Objective: To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel ... ...

    Abstract Objective: To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel regenerative medicine cell-based therapy.
    Methods: Vocal folds of six female, New Zealand White rabbits were bilaterally injured. Three rabbits had immediate bilateral direct injection of 1 × 10
    Results: hAEC treatment significantly increased the expression of ECM proteins, elastin microfibril interface-located protein 1 (EMILIN-1) and myocilin that are primarily involved in elastogenesis of blood vessels and granulation tissue. A reactome pathway analysis showed increased activity of the anchoring fibril formation by collagen I and laminin, providing mechanical stability and activation of cell signaling pathways regulating cell function. hAEC increased the abundance of keratin 1 indicating accelerated induction of the differentiation programming of the basal epithelial cells and, thereby, improved barrier function. Lastly, upregulation of Rab GDP dissociation inhibitor indicates that hAEC activate the vesicle endocytic and exocytic pathways, supporting the exosome-mediated activation of cell-matrix and cell-to-cell interactions.
    Conclusions: This pilot study suggests that injection of hAEC into an injured rabbit vocal fold favorably alters ECM composition creating a microenvironment that accelerates differentiation of regenerated epithelium and promotes stabilization of new blood vessels indicative of accelerated and improved repair.
    MeSH term(s) Animals ; Cell Transplantation ; Chromatography, Liquid ; Cicatrix/pathology ; Epithelial Cells/pathology ; Female ; Humans ; Pilot Projects ; Proteomics ; Rabbits ; Tandem Mass Spectrometry ; Vocal Cords
    Language English
    Publishing date 2022-01-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2548671-8
    ISSN 1757-6512 ; 1757-6512
    ISSN (online) 1757-6512
    ISSN 1757-6512
    DOI 10.1186/s13287-022-02701-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The non-linear relationship between sum of 7 skinfolds and fat and lean mass in elite swimmers.

    Mitchell, Lachlan J G / Morris, Kirstin S / Bolam, Kate A / Pritchard-Peschek, Kellie R / Skinner, Tina L / Shephard, Megan E

    Journal of sports sciences

    2020  Volume 38, Issue 20, Page(s) 2307–2313

    Abstract: Body composition can substantially impact elite swimming performance. In practice, changes in fat and lean mass of elite swimmers are estimated using body mass, sum of seven skinfolds (∑7) and lean mass index (LMI). However, LMI may be insufficiently ... ...

    Abstract Body composition can substantially impact elite swimming performance. In practice, changes in fat and lean mass of elite swimmers are estimated using body mass, sum of seven skinfolds (∑7) and lean mass index (LMI). However, LMI may be insufficiently accurate to detect small changes in body composition which could meaningfully impact swimming performance. This study developed equations which estimate dual-energy x-ray absorptiometry (DXA)-derived lean and fat mass using body mass and ∑7 data. Elite Australian swimmers (n = 44; 18 male, 26 female) completed a DXA scan and standardised body mass and ∑7 measurements. Equations to estimate DXA-derived lean and fat mass based on body mass, ∑7 and sex were developed. The relationships between ∑7, body mass and DXA-derived lean and fat mass were non-linear. Fat mass (Adjusted R
    MeSH term(s) Absorptiometry, Photon ; Adolescent ; Adult ; Athletic Performance/physiology ; Australia ; Body Fat Distribution/statistics & numerical data ; Body Mass Index ; Female ; Humans ; Male ; Regression Analysis ; Skinfold Thickness ; Swimming/physiology ; Young Adult
    Language English
    Publishing date 2020-06-19
    Publishing country England
    Document type Journal Article ; Observational Study
    ZDB-ID 995528-8
    ISSN 1466-447X ; 0264-0414
    ISSN (online) 1466-447X
    ISSN 0264-0414
    DOI 10.1080/02640414.2020.1779491
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury

    Yourka D. Tchoukalova / Stephanie R. C. Zacharias / Natalie Mitchell / Cathy Madsen / Cheryl E. Myers / Dina Gadalla / Jessica Skinner / Katarzyna Kopaczka / Roberto Gramignoli / David G. Lott

    Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-

    a pilot study

    2022  Volume 14

    Abstract: Abstract Objective To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel ... ...

    Abstract Abstract Objective To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel regenerative medicine cell-based therapy. Methods Vocal folds of six female, New Zealand White rabbits were bilaterally injured. Three rabbits had immediate bilateral direct injection of 1 × 106 hAEC in 100 µl of saline solution (hAEC) and three with 100 µl of saline solution (controls, CTR). Rabbits were euthanized 6 weeks after injury. Proteomic analyses (in-gel trypsin protein digestion, LC–MS/MS, protein identification using Proteome Discoverer and the Uniprot Oryctolagus cuniculus (Rabbit) proteome) and histological analyses were performed. Results hAEC treatment significantly increased the expression of ECM proteins, elastin microfibril interface-located protein 1 (EMILIN-1) and myocilin that are primarily involved in elastogenesis of blood vessels and granulation tissue. A reactome pathway analysis showed increased activity of the anchoring fibril formation by collagen I and laminin, providing mechanical stability and activation of cell signaling pathways regulating cell function. hAEC increased the abundance of keratin 1 indicating accelerated induction of the differentiation programming of the basal epithelial cells and, thereby, improved barrier function. Lastly, upregulation of Rab GDP dissociation inhibitor indicates that hAEC activate the vesicle endocytic and exocytic pathways, supporting the exosome-mediated activation of cell–matrix and cell-to-cell interactions. Conclusions This pilot study suggests that injection of hAEC into an injured rabbit vocal fold favorably alters ECM composition creating a microenvironment that accelerates differentiation of regenerated epithelium and promotes stabilization of new blood vessels indicative of accelerated and improved repair.
    Keywords Vocal fold ; Wound healing ; Amnionic epithelial cells ; Extracellular matrix ; Proteomics ; Regenerative medicine ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Subject code 610
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Setting up the JBrowse genome browser.

    Skinner, Mitchell E / Holmes, Ian H

    Current protocols in bioinformatics

    2010  Volume Chapter 9, Page(s) Unit 9.13

    Abstract: JBrowse is a Web-based tool for visualizing genomic data. Unlike most other Web-base genome browsers, JBrowse exploits the capabilities of the user's Web browser to make scrolling and zooming fast and smooth. It supports the browsers used by almost all ... ...

    Abstract JBrowse is a Web-based tool for visualizing genomic data. Unlike most other Web-base genome browsers, JBrowse exploits the capabilities of the user's Web browser to make scrolling and zooming fast and smooth. It supports the browsers used by almost all Internet users, and is relatively simple to install. JBrowse can utilize multiple types of data in a variety of common genomic data formats, including genomic feature data in bioperl databases, GFF files, BED files, and quantitative data in wiggle files. This unit describes how to obtain the JBrowse software, set it up on a Linux or Mac OS X computer running as a Web server, and incorporate genome annotation data from multiple sources into JBrowse. After completing the protocols described in this unit, the reader will have a Web site that other users can visit to browse the genomic data.
    MeSH term(s) Databases, Genetic ; Genome ; Genomics/methods ; Internet ; Molecular Sequence Annotation ; Software ; User-Computer Interface
    Language English
    Publishing date 2010-10-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2179022-X
    ISSN 1934-340X ; 1934-3396
    ISSN (online) 1934-340X
    ISSN 1934-3396
    DOI 10.1002/0471250953.bi0913s32
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Readmission rates and associated factors following rib cage injury.

    Baker, Jennifer E / Skinner, Mitchell / Heh, Victor / Pritts, Timothy A / Goodman, Michael D / Millar, D Anderson / Janowak, Christopher F

    The journal of trauma and acute care surgery

    2019  Volume 87, Issue 6, Page(s) 1269–1276

    Abstract: Background: There remains a lack of knowledge about readmission characteristics after sustaining rib fractures. We aimed to determine rates, characteristics, and predictive/protective factors associated with unexpected reevaluation and readmission after ...

    Abstract Background: There remains a lack of knowledge about readmission characteristics after sustaining rib fractures. We aimed to determine rates, characteristics, and predictive/protective factors associated with unexpected reevaluation and readmission after rib cage injury.
    Methods: A retrospective review was performed based on trauma patients evaluated at an urban Level I trauma center from January 2014 to December 2016. Adult patients sustaining blunt trauma with more than one rib fracture or a sternomanubrial fracture were defined as having moderate to severe rib cage injury. Exclusion criteria included penetrating injury, death during initial hospitalization, and only one rib fracture. Reevaluation was defined as presenting at a hospital within 90 days of discharge urgently or emergently. Demographics, injury characteristics, comorbidities, complications, imaging, and readmission data were collected. Univariate and multivariate analysis was performed with a significance of p less than 0.05.
    Results: During the study period, 11,667 patients underwent trauma evaluation, of which 1,717 patients were found to have a moderate to severe rib cage injury. Within 90 days, 397 (23.1%) of patients underwent reevaluation, while 177 (10.3%) required readmission. One hundred forty-two (8.3%) patients were reevaluated specifically for chest-related complaints, and 55 (3.2%) required readmission. On univariate analysis, Injury Severity Score greater than 15, hospital length of stay longer than 7 days, intensive care unit (ICU) length of stay longer than 3 days, a worsened chest x-ray at discharge, a psychiatric comorbidity, a smoking comorbidity, deep vein thrombosis, unplanned readmission to the ICU, and unplanned intubation were higher in the overall reevaluation cohort. On multivariate analysis, age of 15 years to 35 years, Risk Assessment Profile score greater than 8, hypertension, psychiatric comorbidity, current smoker, and unplanned return to the ICU on index admission were predictive of reevaluation of overall reevaluation.
    Conclusion: Moderate to severe rib cage injury is associated with high rates of reevaluation and readmission. Younger patients who smoke and required a return to the ICU are at greater risk for readmission.
    Level of evidence: Level IV, Prognostic and Epidemiologic.
    MeSH term(s) Adult ; Age Factors ; Aged ; Female ; Hospitals, Urban/statistics & numerical data ; Humans ; Injury Severity Score ; Length of Stay ; Male ; Middle Aged ; Ohio/epidemiology ; Patient Readmission/statistics & numerical data ; Retrospective Studies ; Rib Fractures/complications ; Risk Factors ; Smoking/adverse effects ; Trauma Centers/statistics & numerical data ; Wounds, Nonpenetrating/complications
    Language English
    Publishing date 2019-06-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2651070-4
    ISSN 2163-0763 ; 2163-0755
    ISSN (online) 2163-0763
    ISSN 2163-0755
    DOI 10.1097/TA.0000000000002390
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Safety and Molecular-Toxicological Implications of Cannabidiol-Rich Cannabis Extract and Methylsulfonylmethane Co-Administration.

    Kutanzi, Kristy R / Ewing, Laura E / Skinner, Charles M / Quick, Charles M / Kennon-McGill, Stefanie / McGill, Mitchell R / Walker, Larry A / ElSohly, Mahmoud A / Gurley, Bill J / Koturbash, Igor

    International journal of molecular sciences

    2020  Volume 21, Issue 20

    Abstract: Cannabidiol (CBD) is a biologically active, non-psychotropic component ... ...

    Abstract Cannabidiol (CBD) is a biologically active, non-psychotropic component of
    MeSH term(s) Alkaline Phosphatase/blood ; Animals ; Cannabidiol/pharmacokinetics ; Cannabidiol/toxicity ; Cannabis/chemistry ; Chemical and Drug Induced Liver Injury/blood ; Chemical and Drug Induced Liver Injury/pathology ; Cytochrome P-450 Enzyme System/metabolism ; Dietary Supplements/toxicity ; Glutamine/analogs & derivatives ; Glutamine/metabolism ; Herb-Drug Interactions ; Male ; Mice, Inbred C57BL ; Plant Extracts/chemistry ; Plant Extracts/pharmacokinetics ; Plant Extracts/toxicity ; Taurine/analogs & derivatives ; Taurine/metabolism ; Toxicity Tests
    Chemical Substances Plant Extracts ; Glutamine (0RH81L854J) ; Cannabidiol (19GBJ60SN5) ; Taurine (1EQV5MLY3D) ; Cytochrome P-450 Enzyme System (9035-51-2) ; glutaurine (B5T2Z06Y9N) ; Alkaline Phosphatase (EC 3.1.3.1)
    Language English
    Publishing date 2020-10-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms21207808
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Doxycycline-Coated Silicone Breast Implants Reduce Acute Surgical-Site Infection and Inflammation.

    Baker, Jennifer E / Seitz, Aaron P / Boudreau, Ryan M / Skinner, Mitchell J / Beydoun, Ahmed / Kaval, Necati / Caldwell, Charles C / Gulbins, Erich / Edwards, Michael J / Gobble, Ryan M

    Plastic and reconstructive surgery

    2020  Volume 146, Issue 5, Page(s) 1029–1041

    Abstract: Background: Surgical-site infection after implant-based breast reconstruction remains a leading cause of morbidity. Doxycycline is an antibiotic used to treat soft-tissue infections. The authors hypothesize that doxycycline-coated breast implants will ... ...

    Abstract Background: Surgical-site infection after implant-based breast reconstruction remains a leading cause of morbidity. Doxycycline is an antibiotic used to treat soft-tissue infections. The authors hypothesize that doxycycline-coated breast implants will significantly reduce biofilm formation, surgical-site infection, and inflammation after bacterial infection.
    Methods: Pieces of silicone breast implants were coated in doxycycline. In vitro studies to characterize the coating include Fourier transmission infrared spectroscopy, elution data, and toxicity assays (n = 4). To evaluate antimicrobial properties, coated implants were studied after methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa inoculation in vitro and in a mouse model at 3 and 7 days (n = 8). Studies included bacterial quantification, cytokine profiles, and histology.
    Results: Coated silicone breast implants demonstrated a color change, increased mass, and Fourier transmission infrared spectroscopy consistent with a doxycycline coating. Coated implants were nontoxic to fibroblasts and inhibited biofilm formation and bacterial adherence after MRSA and P. aeruginosa incubation in vitro, and measurable doxycycline concentrations at 24 hours were seen. In a mouse model, a significant reduction of MRSA and P. aeruginosa bacterial colonization after 3 and 7 days in the doxycycline-coated implant mice was demonstrated when compared to the control mice, control mice treated with intraperitoneal doxycycline, and control mice treated with a gentamicin/cefazolin/bacitracin wash. Decreased inflammatory cytokines and inflammatory cell infiltration were demonstrated in the doxycycline-coated mice.
    Conclusions: A method to coat silicone implants with doxycycline was developed. The authors' doxycycline-coated silicone implants significantly reduced biofilm formation, surgical-site infections, and inflammation. Further studies are needed to evaluate the long-term implications.
    MeSH term(s) Acute Disease ; Animals ; Anti-Bacterial Agents/therapeutic use ; Breast Implants ; Coated Materials, Biocompatible/therapeutic use ; Doxycycline/therapeutic use ; Male ; Mastitis/prevention & control ; Methicillin-Resistant Staphylococcus aureus ; Mice ; Postoperative Complications/prevention & control ; Prosthesis Design ; Pseudomonas Infections/prevention & control ; Pseudomonas aeruginosa ; Silicone Gels ; Staphylococcal Infections/prevention & control ; Surgical Wound Infection/prevention & control
    Chemical Substances Anti-Bacterial Agents ; Coated Materials, Biocompatible ; Silicone Gels ; Doxycycline (N12000U13O)
    Language English
    Publishing date 2020-11-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208012-6
    ISSN 1529-4242 ; 0032-1052 ; 0096-8501
    ISSN (online) 1529-4242
    ISSN 0032-1052 ; 0096-8501
    DOI 10.1097/PRS.0000000000007277
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Oropharyngeal cancer outcomes correlate with p16 status, multinucleation and immune infiltration.

    Wilde, David C / Castro, Patricia D / Bera, Kaustav / Lai, Syeling / Madabhushi, Anant / Corredor, German / Koyuncu, Can / Lewis, James S / Lu, Cheng / Frederick, Mitchell J / Frederick, Allan M / Haugen, Avery E / Zevallos, Jose P / Sturgis, Erich M / Shi, Justin / Huang, Andrew T / Hernandez, David J / Skinner, Heath D / Kemnade, Jan O /
    Yu, Wendong / Sikora, Andrew G / Sandulache, Vlad C

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    2022  Volume 35, Issue 8, Page(s) 1045–1054

    Abstract: Oropharyngeal squamous cell carcinoma (OPSCC), largely fueled by the human papillomavirus (HPV), has a complex biological and immunologic phenotype. Although HPV/p16 status can be used to stratify OPSCC patients as a function of survival, it remains ... ...

    Abstract Oropharyngeal squamous cell carcinoma (OPSCC), largely fueled by the human papillomavirus (HPV), has a complex biological and immunologic phenotype. Although HPV/p16 status can be used to stratify OPSCC patients as a function of survival, it remains unclear what drives an improved treatment response in HPV-associated OPSCC and whether targetable biomarkers exist that can inform a precision oncology approach. We analyzed OPSCC patients treated between 2000 and 2016 and correlated locoregional control (LRC), disease-free survival (DFS) and overall survival (OS) with conventional clinical parameters, risk parameters generated using deep-learning algorithms trained to quantify tumor-infiltrating lymphocytes (TILs) (OP-TIL) and multinucleated tumor cells (MuNI) and targeted transcriptomics. P16 was a dominant determinant of LRC, DFS and OS, but tobacco exposure, OP-TIL and MuNI risk features correlated with clinical outcomes independent of p16 status and the combination of p16, OP-TIL and MuNI generated a better stratification of OPSCC risk compared to individual parameters. Differential gene expression (DEG) analysis demonstrated overlap between MuNI and OP-TIL and identified genes involved in DNA repair, oxidative stress response and tumor immunity as the most prominent correlates with survival. Alteration of inflammatory/immune pathways correlated strongly with all risk features and oncologic outcomes. This suggests that development of OPSCC consists of an intersection between multiple required and permissive oncogenic and immunologic events which may be mechanistically linked. The strong relationship between tumor immunity and oncologic outcomes in OPSCC regardless of HPV status may provide opportunities for further biomarker development and precision oncology approaches incorporating immune checkpoint inhibitors for maximal anti-tumor efficacy.
    MeSH term(s) Cyclin-Dependent Kinase Inhibitor p16/analysis ; Head and Neck Neoplasms ; Humans ; Oropharyngeal Neoplasms/pathology ; Papillomaviridae ; Papillomavirus Infections/pathology ; Precision Medicine ; Prognosis ; Squamous Cell Carcinoma of Head and Neck
    Chemical Substances Cyclin-Dependent Kinase Inhibitor p16
    Language English
    Publishing date 2022-02-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 645073-8
    ISSN 1530-0285 ; 0893-3952
    ISSN (online) 1530-0285
    ISSN 0893-3952
    DOI 10.1038/s41379-022-01024-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Hepatotoxicity of a Cannabidiol-Rich Cannabis Extract in the Mouse Model.

    Ewing, Laura E / Skinner, Charles M / Quick, Charles M / Kennon-McGill, Stefanie / McGill, Mitchell R / Walker, Larry A / ElSohly, Mahmoud A / Gurley, Bill J / Koturbash, Igor

    Molecules (Basel, Switzerland)

    2019  Volume 24, Issue 9

    Abstract: The goal of this study was to investigate Cannabidiol (CBD) hepatotoxicity in 8-week-old male ... ...

    Abstract The goal of this study was to investigate Cannabidiol (CBD) hepatotoxicity in 8-week-old male B6C3F
    MeSH term(s) Animals ; Biomarkers ; Cannabidiol/chemistry ; Cannabidiol/pharmacology ; Cannabis/chemistry ; Chemical and Drug Induced Liver Injury/etiology ; Chemical and Drug Induced Liver Injury/metabolism ; Chemical and Drug Induced Liver Injury/pathology ; Disease Models, Animal ; Gene Expression Profiling ; Hepatocytes/drug effects ; Hepatocytes/metabolism ; Liver Function Tests ; Mice ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Transcriptome
    Chemical Substances Biomarkers ; Plant Extracts ; Cannabidiol (19GBJ60SN5)
    Language English
    Publishing date 2019-04-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules24091694
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