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  1. Article ; Online: Influence Of Hulling And Storage Conditions On Maintaining Coffee Quality.

    Abreu, Giselle F / Rosa, Sttela D V F / Coelho, Stefânia V B / Pereira, Cristiane C / Malta, Marcelo R / Fantazzini, Tatiana B / Vilela, Amanda L

    Anais da Academia Brasileira de Ciencias

    2023  Volume 95, Issue 4, Page(s) e20190612

    Abstract: Storage is important in the coffee post-harvest. Determining the maximum period that coffee can remain storaged is important aiming to reduce losses in quality and, consequently, allow the producer to achieve maximum profitability. The aim was to ... ...

    Abstract Storage is important in the coffee post-harvest. Determining the maximum period that coffee can remain storaged is important aiming to reduce losses in quality and, consequently, allow the producer to achieve maximum profitability. The aim was to determine the suitable storage period for natural and fully washed coffees, under different conditions. Beans were dried to 11% moisture content after dry processing (natural coffee, dry cherry coffee) and wet processing (parchment coffee, fully washed). Before storage, part of the coffee was hulled and part was not. The coffee was stored under refrigerated air (10ºC and 50% relative humidity) or in an environment at 25ºC. In the periods of 0, 3, 6, and 12 months, samples were taken for sensory, electrical conductivity and tetrazolium evaluation. Refrigerated environment favors conservation of sensory and physiological quality of the natural hulled coffee beans and fully washed coffee. Hulled beans of natural and fully washed coffee stored under refrigerated conditions have the initial quality conserved for up to 12 months and in non-controlled environmental, for up to 3 months. Mechanical damage caused by hulling, associated with the lack of tissue fruit parts, contributes to reduction hulled coffee quality in storage, regardless of the processing.
    MeSH term(s) Seeds ; Fabaceae
    Language English
    Publishing date 2023-12-11
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2046885-4
    ISSN 1678-2690 ; 0001-3765
    ISSN (online) 1678-2690
    ISSN 0001-3765
    DOI 10.1590/0001-3765202320190612
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Laminin Triggers Neutrophil Extracellular Traps (NETs) and Modulates NET Release Induced by

    Silva-Oliveira, Gustavo / Linhares-Lacerda, Leandra / Mattos, Thayana R F / Sanches, Camila / Coelho-Sampaio, Tatiana / Riederer, Ingo / Saraiva, Elvira M

    Biomedicines

    2022  Volume 10, Issue 3

    Abstract: Neutrophils are recruited from the blood and transmigrate through the endothelium to reach tissues, where they are prone to respond through different mechanisms, including the release of neutrophil extracellular traps (NETs). These responses occur in ... ...

    Abstract Neutrophils are recruited from the blood and transmigrate through the endothelium to reach tissues, where they are prone to respond through different mechanisms, including the release of neutrophil extracellular traps (NETs). These responses occur in close contact with proteins from the basement membrane and extracellular matrix, where laminins are abundant. Thus, we investigated the interactions between neutrophils and different laminin (LM) isoforms and analyzed the induction of NETs. We showed that neutrophils stimulated with LM isoforms 111, 211, 332, 411, 421, and 511 released NETs. The same occurred when neutrophils interacted with polymerized LMs 111, 411, and 511. LM-induced NETs were partially inhibited by pretreatment of neutrophils with an anti-α6 integrin antibody. Furthermore, NETs triggered by laminins were dependent on elastase and peptidylarginine deiminase (PAD)-4, enzymes that participate in chromatin decondensation. We also found that LMs 411 and LM 511 potentiated the NET release promoted by promastigotes of the protozoan parasite
    Language English
    Publishing date 2022-02-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines10030521
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Influence Of Hulling And Storage Conditions On Maintaining Coffee Quality

    GISELLE F. ABREU / STTELA D.V.F. ROSA / STEFÂNIA V.B. COELHO / CRISTIANE C. PEREIRA / MARCELO R. MALTA / TATIANA B. FANTAZZINI / AMANDA L. VILELA

    Anais da Academia Brasileira de Ciências, Vol 95, Iss

    2023  Volume 4

    Abstract: Abstract Storage is important in the coffee post-harvest. Determining the maximum period that coffee can remain storaged is important aiming to reduce losses in quality and, consequently, allow the producer to achieve maximum profitability. The aim was ... ...

    Abstract Abstract Storage is important in the coffee post-harvest. Determining the maximum period that coffee can remain storaged is important aiming to reduce losses in quality and, consequently, allow the producer to achieve maximum profitability. The aim was to determine the suitable storage period for natural and fully washed coffees, under different conditions. Beans were dried to 11% moisture content after dry processing (natural coffee, dry cherry coffee) and wet processing (parchment coffee, fully washed). Before storage, part of the coffee was hulled and part was not. The coffee was stored under refrigerated air (10ºC and 50% relative humidity) or in an environment at 25ºC. In the periods of 0, 3, 6, and 12 months, samples were taken for sensory, electrical conductivity and tetrazolium evaluation. Refrigerated environment favors conservation of sensory and physiological quality of the natural hulled coffee beans and fully washed coffee. Hulled beans of natural and fully washed coffee stored under refrigerated conditions have the initial quality conserved for up to 12 months and in non-controlled environmental, for up to 3 months. Mechanical damage caused by hulling, associated with the lack of tissue fruit parts, contributes to reduction hulled coffee quality in storage, regardless of the processing.
    Keywords Coffea arabica L ; deterioration ; mechanical damage ; sensory analysis ; Science ; Q
    Subject code 910
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Academia Brasileira de Ciências
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Combining local antibiotic delivery with heparinized nanohydroxyapatite/collagen bone substitute: A novel strategy for osteomyelitis treatment.

    Padrão, Tatiana / Coelho, Catarina C / Costa, Paulo / Alegrete, Nuno / Monteiro, Fernando J / Sousa, Susana R

    Materials science & engineering. C, Materials for biological applications

    2020  Volume 119, Page(s) 111329

    Abstract: Osteomyelitis is a major challenge in bone surgery and conventional treatment is frequently ineffective to control the infection, with an alternative approach being required. In the present work, a heparinized nanohydroxyapatite/collagen biocomposite was ...

    Abstract Osteomyelitis is a major challenge in bone surgery and conventional treatment is frequently ineffective to control the infection, with an alternative approach being required. In the present work, a heparinized nanohydroxyapatite/collagen biocomposite was produced in granular form, and loaded with vancomycin, to work as a local drug delivery system for osteomyelitis and as a bone substitute. This strategy involves the local delivery of high concentrations of vancomycin, to eradicate the infection. Additionally, these granules work as a scaffold with regenerative properties, to induce bone regeneration after antibiotic release. The heparinized nanohydroxyapatite/collagen granular bone substitute was produced using two different sintering temperatures to study their effect on granules properties and on vancomycin release profile. Morphological, topographic, chemical and mechanical characterization were carried out for granules sintered at both temperatures and some relevant differences were found. The mechanical strength was increased by several orders of magnitude with increasing sintering temperature, being able to maintain their porous macrostructure and withstand important processes for their commercialization such as packaging, shipping and surgical manipulation. The nanohydroxyapatite/collagen granules were able to release high concentrations of vancomycin, always above MIC, for 19 days. The released antibiotic was able to eradicate both planktonic and sessile methicillin-resistant Staphylococcus aureus. The cytotoxicity was assessed according to ISO 10993-5:2009 and the granules sintered at higher temperature showed no cytotoxic effect. Considering these results nanohydroxyapatite/collagen biocomposite loaded with vancomycin is a promising solution for osteomyelitis treatment.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Bone Substitutes/pharmacology ; Collagen ; Humans ; Methicillin-Resistant Staphylococcus aureus ; Osteomyelitis/drug therapy ; Staphylococcal Infections
    Chemical Substances Anti-Bacterial Agents ; Bone Substitutes ; Collagen (9007-34-5)
    Language English
    Publishing date 2020-08-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2012160-X
    ISSN 1873-0191 ; 0928-4931
    ISSN (online) 1873-0191
    ISSN 0928-4931
    DOI 10.1016/j.msec.2020.111329
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Increased Serum Mir-150-3p Expression Is Associated with Radiological Lung Injury Improvement in Patients with COVID-19.

    Bueno, Larissa C M / Paim, Layde R / Minin, Eduarda O Z / da Silva, Luís Miguel / Mendes, Paulo R / Kiyota, Tatiana A / Schreiber, Angelica Z / Bombassaro, Bruna / Mansour, Eli / Moretti, Maria Luiza / Chow, Jonathan Tak-Sum / Salmena, Leonardo / Coelho-Filho, Otavio R / Velloso, Licio A / Nadruz, Wilson / Schreiber, Roberto

    Viruses

    2022  Volume 14, Issue 7

    Abstract: Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus, responsible for an atypical pneumonia that can progress to acute lung injury. MicroRNAs are small non-coding RNAs that control specific genes and pathways. This study evaluated the ... ...

    Abstract Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus, responsible for an atypical pneumonia that can progress to acute lung injury. MicroRNAs are small non-coding RNAs that control specific genes and pathways. This study evaluated the association between circulating miRNAs and lung injury associated with COVID-19.
    MeSH term(s) COVID-19/genetics ; Computational Biology/methods ; Humans ; Lung Injury ; MicroRNAs/metabolism ; SARS-CoV-2
    Chemical Substances MIRN150 microRNA, human ; MIRN212 microRNA, human ; MicroRNAs
    Language English
    Publishing date 2022-06-23
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14071363
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Synthesis and Biological Evaluation of Cyclic Analogues from Nitrone LQB-278: A New Potential Antileukemia Compound.

    Thimoteo, Rachell R C / Costa, Debora S S / Martino, Thiago / Barcellos, Julio C F / Coelho, Marsen G P / Costa, Paulo R R / Sabino, Katia C C / Simao, Tatiana / Dias, Ayres G / Justo, Graça

    Anticancer research

    2021  Volume 41, Issue 10, Page(s) 4929–4936

    Abstract: Background/aim: A new set of LQB-nitrones and analogues was synthesized to evaluate anticancer activity based on the substitution of the terpenyl moiety of the antileukemic compound LQB-278 by the conformationally restricted cinnamyl ether.: Materials ...

    Abstract Background/aim: A new set of LQB-nitrones and analogues was synthesized to evaluate anticancer activity based on the substitution of the terpenyl moiety of the antileukemic compound LQB-278 by the conformationally restricted cinnamyl ether.
    Materials and methods: A structure-activity relationship study was performed in vitro on Jurkat cells to screen the antileukemic activity of LQB-nitrones and analogues and elucidate the mechanisms of action of the most active derivatives.
    Results: The cynamyl ramification and its ortho position aldehyde substitution improved the antileukemic activity. Three compounds showed an in vitro antiproliferative action, but only
    Conclusion: The cinnamyl derivative
    MeSH term(s) Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/pharmacology ; Apoptosis ; Cell Proliferation ; Humans ; Imines/chemistry ; Leukemia/drug therapy ; Leukemia/pathology ; Nitrogen Oxides/chemistry ; Nitrogen Oxides/pharmacology ; Tumor Cells, Cultured
    Chemical Substances Antineoplastic Agents ; Imines ; LQB-278 ; Nitrogen Oxides ; nitrones
    Language English
    Publishing date 2021-09-30
    Publishing country Greece
    Document type Evaluation Study ; Journal Article
    ZDB-ID 604549-2
    ISSN 1791-7530 ; 0250-7005
    ISSN (online) 1791-7530
    ISSN 0250-7005
    DOI 10.21873/anticanres.15306
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Polymerized laminin incorporation into alginate-based microcapsules reduces pericapsular overgrowth and inflammation.

    Leal-Lopes, Camila / Grazioli, Gisella / Mares-Guia, Thiago R / Coelho-Sampaio, Tatiana / Sogayar, Mari Cleide

    Journal of tissue engineering and regenerative medicine

    2019  Volume 13, Issue 10, Page(s) 1912–1922

    Abstract: Cell encapsulation coats cells with an artificial membrane to preserve their physical and functional integrity. Different approaches try to develop more functional and biocompatible materials to avoid cell loss after transplantation due to inflammatory ... ...

    Abstract Cell encapsulation coats cells with an artificial membrane to preserve their physical and functional integrity. Different approaches try to develop more functional and biocompatible materials to avoid cell loss after transplantation due to inflammatory reaction, one of the main causes for graft failure. In this study, the LN-Biodritin biomaterial, based on alginate, chondroitin sulfate, and laminin, previously developed by our group, was further improved by replacing laminin by polylaminin, an artificial laminin polymer with anti-inflammatory properties, generating the new biomaterial polyLN-Biodritin. Capsules containing polylaminin are stable, do not induce macrophage activation in vitro, and are also able to prevent macrophage activation by encapsulated human pancreatic islets in vitro, preserving their glucose-stimulated insulin secretion potential. In addition, when empty capsules containing polylaminin were implanted into immunocompetent mice, the inflammatory response towards the implant was attenuated, when compared with capsules without polylaminin. The results indicate that polylaminin incorporation leads to lower levels of pericapsular growth on the capsules surface, lower infiltration of cells into the peritoneal cavity, and lower production of proinflammatory cytokines, both at the implant site (interleukin-12p70 (IL-12p70), tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1), and interferon-γ (IFN-γ)) and systemically (IL-12p70 and TNF-α). Therefore, polylaminin incorporation into the microcapsules polymer attenuates the host posttransplantation immune response against implanted microcapsules, being likely to favor maintenance of engrafted encapsulated cells.
    MeSH term(s) Alginates/chemistry ; Animals ; Biocompatible Materials/pharmacology ; Capsules ; Cytokines/metabolism ; Humans ; Inflammation/pathology ; Inflammation Mediators/metabolism ; Islets of Langerhans/drug effects ; Islets of Langerhans/metabolism ; Laminin/pharmacology ; Macrophage Activation/drug effects ; Macrophages/drug effects ; Macrophages/metabolism ; Mice ; Mice, Inbred BALB C ; Polymerization ; RAW 264.7 Cells
    Chemical Substances Alginates ; Biocompatible Materials ; Capsules ; Cytokines ; Inflammation Mediators ; Laminin
    Language English
    Publishing date 2019-08-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-7005
    ISSN (online) 1932-7005
    DOI 10.1002/term.2942
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Excess adiposity and low physical fitness hamper Supine-to-Stand test performance among sedentary adolescents.

    Tadiotto, Maiara C / Duncan, Michael / Mota, Jorge / Moraes-Junior, Frederico B / Corazza, Patricia R P / Czoczuk, Matheus / Menezes-Junior, Francisco J de / Tozo, Tatiana A A / Coelho-E-Silva, Manuel J / Rodacki, André L F / Leite, Neiva

    Jornal de pediatria

    2021  Volume 97, Issue 6, Page(s) 658–664

    Abstract: Objective: The aim of this study was to analyze the relationship between body adiposity and physical fitness with performance in the Supine-to-Stand test (STS-test) in sedentary adolescents.: Methods: Sixty-two adolescents, of both sexes, between 10 ... ...

    Abstract Objective: The aim of this study was to analyze the relationship between body adiposity and physical fitness with performance in the Supine-to-Stand test (STS-test) in sedentary adolescents.
    Methods: Sixty-two adolescents, of both sexes, between 10 and 16 years old, participated in the study. Body mass (BM), height, waist circumference (WC), fat mass (FM), fat-free mass (FFM), right and left handgrip strength (HGS-right, HGS-left), abdominal resistance (ABDO), flexibility (FLEX), and cardiorespiratory fitness (VO
    Results: The SG presented higher BMI, BMI-z, TMI, WHtR, FM, %FM, as well as lower averages for %FFM, HGS-right, HGS-left, FLEX, ABDO, VO
    Conclusion: It is concluded that excess fat and low physical fitness hamper STS-test performance. Therefore, the STS-test can be used for screening students to assess MC.
    MeSH term(s) Adiposity ; Adolescent ; Body Mass Index ; Child ; Cross-Sectional Studies ; Female ; Hand Strength ; Humans ; Male ; Physical Fitness ; Waist Circumference
    Language English
    Publishing date 2021-03-11
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 731324-x
    ISSN 1678-4782 ; 0021-7557
    ISSN (online) 1678-4782
    ISSN 0021-7557
    DOI 10.1016/j.jped.2021.01.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Trypanosoma cruzi

    Nisimura, Lindice M / Coelho, Laura L / de Melo, Tatiana G / Vieira, Paloma de Carvalho / Victorino, Pedro H / Garzoni, Luciana R / Spray, David C / Iacobas, Dumitru A / Iacobas, Sanda / Tanowitz, Herbert B / Adesse, Daniel

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 255

    Abstract: Chagas disease is responsible for more than 10,000 deaths per year and about 6 to 7 million infected people worldwide. In its chronic stage, patients can develop mega-colon, mega-esophagus, and cardiomyopathy. Differences in clinical outcomes may be ... ...

    Abstract Chagas disease is responsible for more than 10,000 deaths per year and about 6 to 7 million infected people worldwide. In its chronic stage, patients can develop mega-colon, mega-esophagus, and cardiomyopathy. Differences in clinical outcomes may be determined, in part, by the genetic background of the parasite that causes Chagas disease.
    MeSH term(s) Animals ; Brazil ; Cell Cycle ; Humans ; Myoblasts ; Rats ; Signal Transduction ; Transcriptome ; Trypanosoma cruzi/genetics
    Language English
    Publishing date 2020-06-17
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.00255
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Eugenol Ester Derivatives

    José R. A. Coelho / Tatiana F. Vieira / Renato B. Pereira / David M. Pereira / Elisabete M. S. Castanheira / A. Gil Fortes / Sérgio F. Sousa / Maria José G. Fernandes / M. Sameiro T. Gonçalves

    Chemistry Proceedings, Vol 8, Iss 83, p

    Synthesis, Insecticidal Activity and Computational Studies

    2021  Volume 83

    Abstract: Specific structural modifications in eugenol molecules can simultaneously improve the biological activity and reduce side effects of the respective analogues. The esterification of eugenol by two different experimental procedures, and subsequently the ... ...

    Abstract Specific structural modifications in eugenol molecules can simultaneously improve the biological activity and reduce side effects of the respective analogues. The esterification of eugenol by two different experimental procedures, and subsequently the conversion of one of the esters into the corresponding oxirane, was carried out. All derivatives obtained were then evaluated for their effect on the viability of Sf9 ( Spodoptera frugiperda ) cells. In addition, a structured-based inverted virtual screening protocol was employed to identify the potential proteins associated with the observed insecticidal activity. The encouraging results obtained allowed us to establish a preliminary structure–activity relationship.
    Keywords eugenol ; eugenol derivatives ; Spodoptera frugiperda ; insecticidal activity ; computational studies ; Chemistry ; QD1-999
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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