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Article: Tests for aggregated dispersion: Van Valen’s test and a new competitor

Livingston, Glen, Jr / Allingham, David / Rayner, J. C. W.

Environmental and ecological statistics. 2022 June, v. 29, no. 2

2022

Abstract: Van Valen’s test is usually applied as a two sample test for equality of dispersion ... for multivariate data. Motivated by a comment of Manly (Van Valen’s test. Encyclopedia of Statistical Sciences ... 2006) that “Little is known about the properties of Van Valen’s test” we develop an alternative test ...

Abstract	Van Valen’s test is usually applied as a two sample test for equality of dispersion for multivariate data. Motivated by a comment of Manly (Van Valen’s test. Encyclopedia of Statistical Sciences, 2006) that “Little is known about the properties of Van Valen’s test” we develop an alternative test and compare the Van Valen test with our alternative robust test in an extensive simulation study. We find that Van Valen’s test does not actually test for equality of variance sums; however, for that null hypothesis it still performs well in terms of closeness to the nominal significance level. Due to testing the correct null hypothesis and the excellent adherence to the nominal significance level, we recommend the use of the robust test as a permutation test.
Keywords	dispersions ; sampling ; testing ; variance
Language	English
Dates of publication	2022-06
Size	p. 223-239.
Publishing place	Springer US
Document type	Article
ZDB-ID	2000906-9
ISSN	1573-3009 ; 1352-8505
ISSN (online)	1573-3009
ISSN	1352-8505
DOI	10.1007/s10651-021-00517-0
Database	NAL-Catalogue (AGRICOLA)

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Book: The Independent Mind in British Psychoanalysis

Rayner, Eric

(Routledge Mental Health Classic Editions)

2020

Abstract: ... psychoanalysis, Eric Rayner’s The Independent Mind in British Psychoanalysis offers a coherent account ...

Author's details	Eric Rayner was a Psychoanalyst, a Training Analyst and a Distinguished Fellow of the British Psychoanalytic Society. His publications included the classic Human Development and Unconscious Logic: An Introduction to Matte Blancös Bi-Logic and its Uses
Series title	Routledge Mental Health Classic Editions
Abstract	Arguably the most informative and readable account of the development of British independent psychoanalysis, Eric Rayner’s The Independent Mind in British Psychoanalysis offers a coherent account of the core concepts that influence the clinical practice. Covering the main themes and theorists with rigour and clarity, it has rightly found a central place on the reading lists of psychoanalytic and psychotherapy trainings, both in the UK and worldwide. -- -- Republished with a new foreword fro...
Keywords	British ; Eric ; Independent ; mind ; psychoanalysis ; psychotherapy ; Rayner ; Vice Versa ; British Psychoanalysis ; British Psycho Analytical Society ; Psycho Analytical Society ; Younger Men ; Anna Freudians ; London Psycho Analytical Society ; British Independent Analyst ; Benign Regression ; social workers ; British Independents ; psychotherapists ; Schizoid Personality ; British independent psychoanalysis ; Basic Fault Level ; Analyst’s Emotional Reactions ; Ego Nuclei ; Peper Harow ; Functional Symbolism ; Locomotor Activity ; Psychoanalytic Symbolism ; Death Instinct Theory ; Analyst’s Affective Responsiveness ; Instinctual Strivings ; Analyst’s Counter-transference ; Polymorphous Perverse Infantile Sexuality ; Transference Interpretation ; Organism’s Central Nervous System
Language	English
Size	268 p.
Edition	1
Publisher	Taylor & Francis Ltd
Document type	Book
Note	PDA Manuell_5
Format	156 x 234 x 18
ISBN	9780367371340 ; 0367371340
Database	PDA

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Article ; Online: Statewide survey of medically important ticks on white-tailed deer, Odocoileus virginianus Zimmerman, in Alabama, U.S.A

Kerr, Skyler M. / Rayner, Jonathan O. / Wood, R. Ryan / McCreadie, John

Journal of Vector Ecology. 2022 Oct. 18, v. 47, no. 2 p.210-216

2022

Abstract: A statewide survey of the tick fauna found on deer, using harvested deer heads as the sample unit, was conducted during the Alabama hunting seasons of 2019-2020 and 2020-2021. Four species of ticks: Ixodes scapularis (n= 936, % of catch 69.1%), Amblyomma ...

Abstract	A statewide survey of the tick fauna found on deer, using harvested deer heads as the sample unit, was conducted during the Alabama hunting seasons of 2019-2020 and 2020-2021. Four species of ticks: Ixodes scapularis (n= 936, % of catch 69.1%), Amblyomma americanum (315, 23.2%), Dermacentor albipictus (97, 7.1%), and Amblyomma maculatum (6, 0.4%) were taken from 151 deer heads harvested from 21 deer processing centers (n = 4-17 heads/processor). A total of 87.7% (prevalence) of deer heads had one or more ticks. We used two standard numerical descriptors of tick numbers, abundance, and relative abundance. No significant stepwise regressions (p > 0.05) were found between tick abundance (all ticks, I. scapularis, A. americanum) and the predictor variables of latitude, deer density, season, and year. In addition, the correlation between the abundance of I. scapularis and A. americanum was not significant (p > 0.5). In contrast, the relative abundance of both I. scapularis and A. americanum showed a significant (P < 0.05) relationship with latitude, with the relative abundance of I. scapularis increasing on deer with increased latitude and A. americanum showing the opposite pattern.
Keywords	Amblyomma americanum ; Amblyomma maculatum ; Dermacentor albipictus ; Ixodes scapularis ; Odocoileus virginianus ; deer ; fauna ; latitude ; surveys ; ticks ; Alabama ; White-tailed deer
Language	English
Dates of publication	2022-1018
Size	p. 210-216.
Publishing place	Society for Vector Ecology
Document type	Article ; Online
ZDB-ID	2212806-2
ISSN	1948-7134 ; 1081-1710
ISSN (online)	1948-7134
ISSN	1081-1710
DOI	10.52707/1081-1710-47.2.210
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Search for a dark matter annihilation signal from the galactic center halo with H.E.S.S.

Abramowski, A / Acero, F / Aharonian, F / Akhperjanian, A G / Anton, G / Barnacka, A / de Almeida, U Barres / Bazer-Bachi, A R / Becherini, Y / Becker, J / Behera, B / Bernlöhr, K / Bochow, A / Boisson, C / Bolmont, J / Bordas, P / Borrel, V / Brucker, J / Brun, F /

Brun, P / Bulik, T / Büsching, I / Carrigan, S / Casanova, S / Cerruti, M / Chadwick, P M / Charbonnier, A / Chaves, R C G / Cheesebrough, A / Chounet, L-M / Clapson, A C / Coignet, G / Conrad, J / Dalton, M / Daniel, M K / Davids, I D / Degrange, B / Deil, C / Dickinson, H J / Djannati-Ataï, A / Domainko, W / Drury, L O'C / Dubois, F / Dubus, G / Dyks, J / Dyrda, M / Egberts, K / Eger, P / Espigat, P / Fallon, L / Farnier, C / Fegan, S / Feinstein, F / Fernandes, M V / Fiasson, A / Fontaine, G / Förster, A / Füssling, M / Gallant, Y A / Gast, H / Gérard, L / Gerbig, D / Giebels, B / Glicenstein, J F / Glück, B / Goret, P / Göring, D / Hague, J D / Hampf, D / Hauser, M / Heinz, S / Heinzelmann, G / Henri, G / Hermann, G / Hinton, J A / Hoffmann, A / Hofmann, W / Hofverberg, P / Horns, D / Jacholkowska, A / de Jager, O C / Jahn, C / Jamrozy, M / Jung, I / Kastendieck, M A / Katarzyński, K / Katz, U / Kaufmann, S / Keogh, D / Kerschhaggl, M / Khangulyan, D / Khélifi, B / Klochkov, D / Kluźniak, W / Kneiske, T / Komin, Nu / Kosack, K / Kossakowski, R / Laffon, H / Lamanna, G / Lennarz, D / Lohse, T / Lopatin, A / Lu, C-C / Marandon, V / Marcowith, A / Masbou, J / Maurin, D / Maxted, N / McComb, T J L / Medina, M C / Méhault, J / Moderski, R / Moulin, E / Naumann, C L / Naumann-Godo, M / de Naurois, M / Nedbal, D / Nekrassov, D / Nguyen, N / Nicholas, B / Niemiec, J / Nolan, S J / Ohm, S / Olive, J-F / Wilhelmi, E de Oña / Opitz, B / Ostrowski, M / Panter, M / Arribas, M Paz / Pedaletti, G / Pelletier, G / Petrucci, P-O / Pita, S / Pühlhofer, G / Punch, M / Quirrenbach, A / Raue, M / Rayner, S M / Reimer, A / Reimer, O / Renaud, M / de los Reyes, R / Rieger, F / Ripken, J / Rob, L / Rosier-Lees, S / Rowell, G / Rudak, B / Rulten, C B / Ruppel, J / Ryde, F / Sahakian, V / Santangelo, A / Schlickeiser, R / Schöck, F M / Schönwald, A / Schwanke, U / Schwarzburg, S / Schwemmer, S / Shalchi, A / Sikora, M / Skilton, J L / Sol, H / Spengler, G / Stawarz, Ł / Steenkamp, R / Stegmann, C / Stinzing, F / Sushch, I / Szostek, A / Tavernet, J-P / Terrier, R / Tibolla, O / Tluczykont, M / Valerius, K / van Eldik, C / Vasileiadis, G / Venter, C / Vialle, J P / Viana, A / Vincent, P / Vivier, M / Völk, H J / Volpe, F / Vorobiov, S / Vorster, M / Wagner, S J / Ward, M / Wierzcholska, A / Zajczyk, A / Zdziarski, A A / Zech, A / Zechlin, H-S

Physical review letters

2011 Volume 106, Issue 16, Page(s) 161301

Abstract: ... The background-subtracted γ-ray spectrum measured with the High Energy Stereoscopic System (H.E.S.S.) γ-ray ... In particular, for the DM particle mass of ∼1 TeV, values for (σv) above 3×10(-25) cm(3) s(-1) are excluded ...

Abstract	A search for a very-high-energy (VHE; ≥100 GeV) γ-ray signal from self-annihilating particle dark matter (DM) is performed towards a region of projected distance r∼45-150 pc from the Galactic center. The background-subtracted γ-ray spectrum measured with the High Energy Stereoscopic System (H.E.S.S.) γ-ray instrument in the energy range between 300 GeV and 30 TeV shows no hint of a residual γ-ray flux. Assuming conventional Navarro-Frenk-White and Einasto density profiles, limits are derived on the velocity-weighted annihilation cross section (σv) as a function of the DM particle mass. These are among the best reported so far for this energy range and in particular differ only little between the chosen density profile parametrizations. In particular, for the DM particle mass of ∼1 TeV, values for (σv) above 3×10(-25) cm(3) s(-1) are excluded for the Einasto density profile.
Language	English
Publishing date	2011-04-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.106.161301
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Article ; Online: A systematic literature review of the influence of the university’s environment and support system on the precursors of social entrepreneurial intention of students

Carlos Bazan / Hannah Gaultois / Arifusalam Shaikh / Katie Gillespie / Sean Frederick / Ali Amjad / Simon Yap / Chantel Finn / James Rayner / Nafisa Belal

Journal of Innovation and Entrepreneurship, Vol 9, Iss 1, Pp 1-

2020 Volume 28

Abstract: Abstract This systematic literature review aims at understanding the influence of the university’s ... approaches to evaluate the impact of various motivational factors related to the university’s entrepreneurial ...

Abstract	Abstract This systematic literature review aims at understanding the influence of the university’s environment and support system (ESS) in shaping the social entrepreneurial intention (SEI) of post-secondary education students. Social entrepreneurs play an important role in the economic and social developments of the communities in which they operate, thus many post-secondary institutions are starting to encourage more students to engage in social entrepreneurial behaviour. Consequently, there is a need for systematic approaches to evaluate the impact of various motivational factors related to the university’s entrepreneurial ecosystem that could affect the SEI of students. Based on a systematic literature review and narrative synthesis of the antecedents of the SEI of post-secondary education students, the authors proposed a customized SEI model that modifies and extend the one proposed by Hockerts (Entrepreneurship: Theory and Practice, 2017) and Mair and Noboa (Social entrepreneurship, 2006). This study fills a gap in the literature by providing a methodology grounded in theory that can help universities to design their educational and other interventions aimed at encouraging more students to consider social entrepreneurship as a viable career choice after graduation.
Keywords	Social entrepreneurial intention ; University environment and support system ; Student social entrepreneurs ; Systematic literature review ; Business ; HF5001-6182 ; Commercial geography. Economic geography ; HF1021-1027
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	SpringerOpen
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Global analysis of apicomplexan protein S-acyl transferases reveals an enzyme essential for invasion.

Frénal, Karine / Tay, Chwen L / Mueller, Christina / Bushell, Ellen S / Jia, Yonggen / Graindorge, Arnault / Billker, Oliver / Rayner, Julian C / Soldati-Favre, Dominique

Traffic (Copenhagen, Denmark)

2013 Volume 14, Issue 8, Page(s) 895–911

Abstract: ... export and organelle biogenesis. However, nothing is known so far about the repertoire of protein S ...

Abstract	The advent of techniques to study palmitoylation on a whole proteome scale has revealed that it is an important reversible modification that plays a role in regulating multiple biological processes. Palmitoylation can control the affinity of a protein for lipid membranes, which allows it to impact protein trafficking, stability, folding, signalling and interactions. The publication of the palmitome of the schizont stage of Plasmodium falciparum implicated a role for palmitoylation in host cell invasion, protein export and organelle biogenesis. However, nothing is known so far about the repertoire of protein S-acyl transferases (PATs) that catalyse this modification in Apicomplexa. We undertook a comprehensive analysis of the repertoire of Asp-His-His-Cys cysteine-rich domain (DHHC-CRD) PAT family in Toxoplasma gondii and Plasmodium berghei by assessing their localization and essentiality. Unlike functional redundancies reported in other eukaryotes, some apicomplexan-specific DHHCs are essential for parasite growth, and several are targeted to organelles unique to this phylum. Of particular interest is DHHC7, which localizes to rhoptry organelles in all parasites tested, including the major human pathogen P. falciparum. TgDHHC7 interferes with the localization of the rhoptry palmitoylated protein TgARO and affects the apical positioning of the rhoptry organelles. This PAT has a major impact on T. gondii host cell invasion, but not on the parasite's ability to egress.
MeSH term(s)	Acetyltransferases/chemistry ; Acetyltransferases/genetics ; Acetyltransferases/metabolism ; Amino Acid Motifs ; Cell Culture Techniques ; Gene Deletion ; Genome, Protozoan ; Humans ; Phylogeny ; Plasmodium berghei/enzymology ; Plasmodium berghei/pathogenicity ; Protein Structure, Tertiary ; Protein Transport ; Protozoan Proteins/chemistry ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Toxoplasma/enzymology ; Toxoplasma/pathogenicity
Chemical Substances	Protozoan Proteins ; Acetyltransferases (EC 2.3.1.-) ; protein acyltransferase (EC 2.3.1.-)
Language	English
Publishing date	2013-05-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1483852-7
ISSN	1600-0854 ; 1398-9219
ISSN (online)	1600-0854
ISSN	1398-9219
DOI	10.1111/tra.12081
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Article: Mild extraction methods using aqueous glucose solution for the analysis of natural dyes in textile artefacts dyed with Dyerâs madder (Rubia tinctorum L.)

Ford, Lauren / Christopher M. Rayner / Richard S. Blackburn / Robert L. Henderson

Journal of chromatography. 2017 Mar. 03, v. 1487

2017

Abstract: Madder (Rubia tinctorum L.) has been widely used as a red dye throughout history. Acid-sensitive colorants present in madder, such as glycosides (lucidin primeveroside, ruberythric acid, galiosin) and sensitive aglycons (lucidin), are degraded in the ... ...

Abstract	Madder (Rubia tinctorum L.) has been widely used as a red dye throughout history. Acid-sensitive colorants present in madder, such as glycosides (lucidin primeveroside, ruberythric acid, galiosin) and sensitive aglycons (lucidin), are degraded in the textile back extraction process; in previous literature these sensitive molecules are either absent or present in only low concentrations due to the use of acid in typical textile back extraction processes. Anthraquinone aglycons alizarin and purpurin are usually identified in analysis following harsh back extraction methods, such those using solvent mixtures with concentrated hydrochloric acid at high temperatures. Use of softer extraction techniques potentially allows for dye components present in madder to be extracted without degradation, which can potentially provide more information about the original dye profile, which varies significantly between madder varieties, species and dyeing technique. Herein, a softer extraction method involving aqueous glucose solution was developed and compared to other back extraction techniques on wool dyed with root extract from different varieties of Rubia tinctorum. Efficiencies of the extraction methods were analysed by HPLC coupled with diode array detection. Acidic literature methods were evaluated and they generally caused hydrolysis and degradation of the dye components, with alizarin, lucidin, and purpurin being the main compounds extracted. In contrast, extraction in aqueous glucose solution provides a highly effective method for extraction of madder dyed wool and is shown to efficiently extract lucidin primeveroside and ruberythric acid without causing hydrolysis and also extract aglycons that are present due to hydrolysis during processing of the plant material. Glucose solution is a favourable extraction medium due to its ability to form extensive hydrogen bonding with glycosides present in madder, and displace them from the fibre. This new glucose method offers an efficient process that preserves these sensitive molecules and is a step-change in analysis of madder dyed textiles as it can provide further information about historical dye preparation and dyeing processes that current methods cannot. The method also efficiently extracts glycosides in artificially aged samples, making it applicable for museum textile artefacts.
Keywords	alizarin ; fabrics ; glucose ; glycosides ; high performance liquid chromatography ; hydrochloric acid ; hydrogen bonding ; hydrolysis ; natural dyes ; Rubia tinctorum ; solvents ; temperature ; wool
Language	English
Dates of publication	2017-0303
Size	p. 36-46.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2017.01.053
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Article: Regulation of vascular tone by S-nitroso-myoglobin.

Rayner, Benjamin S / Wu, Ben J / Raftery, Mark / Stocker, Roland / Witting, Paul K

Redox report : communications in free radical research

2004 Volume 9, Issue 6, Page(s) 382–386

Abstract: ... endothelium-derived relaxant factor - nitric oxide ((.-)NO) - via formation of both nitrosyl-haem iron and S-nitroso-myoglobin (S-NO ... Mb). S-NO-Mb represents a novel form of endothelium-derived relaxant factor (EDRF) that may be ... to show that: (i) S-nitrosation of oxygenated ferrous myoglobin (oxyMb) can compete with the rapid ...

Abstract	Myoglobin (Mb) is a haem protein present in skeletal, cardiac and smooth muscle where it facilitates the transfer of O(2) from the extracellular matrix to the cell cytosol in a cycle termed 'facilitated O(2)-diffusion'. In addition, we showed recently that recombinant human Mb binds endothelium-derived relaxant factor - nitric oxide ((.-)NO) - via formation of both nitrosyl-haem iron and S-nitroso-myoglobin (S-NO-Mb). S-NO-Mb represents a novel form of endothelium-derived relaxant factor (EDRF) that may be important in maintaining optimal (.-)NO concentrations in the human vasculature. In this study we aim to show that: (i) S-nitrosation of oxygenated ferrous myoglobin (oxyMb) can compete with the rapid oxidation of (.-)NO by oxyMb; and (ii) S-NO-Mb retains characteristics of physiological EDRF.
MeSH term(s)	Animals ; Aorta ; Endothelium-Dependent Relaxing Factors/chemical synthesis ; Endothelium-Dependent Relaxing Factors/pharmacology ; Humans ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/physiology ; Myoglobin/chemical synthesis ; Myoglobin/chemistry ; Myoglobin/pharmacology ; Nitrosation ; Rabbits ; S-Nitrosothiols/chemistry ; Vasodilation/drug effects
Chemical Substances	Endothelium-Dependent Relaxing Factors ; Myoglobin ; S-Nitrosothiols ; myoglobin nitroxide ; oxymyoglobin
Language	English
Publishing date	2004
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1305290-1
ISSN	1743-2928 ; 1351-0002
ISSN (online)	1743-2928
ISSN	1351-0002
DOI	10.1179/135100004225006920
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Article ; Online: Computational evolutionary analysis of the overlapped surface (S) and polymerase (P) region in hepatitis B virus indicates the spacer domain in P is crucial for survival.

Chen, Ping / Gan, Yun / Han, Na / Fang, Wei / Li, Jiafu / Zhao, Fei / Hu, Kanghong / Rayner, Simon

PloS one

2013 Volume 8, Issue 4, Page(s) e60098

Abstract: Introduction: The Hepatitis B Virus (HBV) genome contains four ORFs, S (surface), P (polymerase ... C (core) and X. S is completely overlapped by P and as a consequence the overlapping region is ... secondary structure regions. Our results indicate: (i) conservation in S is primarily dictated by α-helix ...

Abstract	Introduction: The Hepatitis B Virus (HBV) genome contains four ORFs, S (surface), P (polymerase), C (core) and X. S is completely overlapped by P and as a consequence the overlapping region is subject to distinctive evolutionary constraints compared to the remainder of the genome. Specifically, a non-synonymous substitution in one coding frame may produce a synonymous substitution in the alternative frame, suggesting a possible conflict between requirements for diversifying and purifying forces. To examine how these contrasting requirements are balanced within this region, we investigated the relationship amongst positive selection sites, conserved regions, epitopes and elements of protein structure to consider how HBV balances the contrasting evolutionary pressures. Methodology/results: 323 HBV genotype D genome sequences were collected and analyzed to identify sites under positive selection and highly conserved regions. Epitopes sequences were retrieved from previously published experimental studies stored in the Immune Epitope Database. Predicted secondary structures were used to investigate the association between structure and conservation. Entropy was used as a measure of conservation and bivariate logistic regression was used to investigate the relationship between positive selection/conserved sites and epitope/secondary structure regions. Our results indicate: (i) conservation in S is primarily dictated by α-helix elements in the protein structure, (ii) variable residues are mainly located in PreS, the major hydrophilic region (MHR) and the C-terminus, (iii) epitopes in S, which are directly targeted by the host immune system, are significantly associated with sites under positive selection. Conclusions: The highly variable spacer domain in P, which corresponds to PreS in S, appears to act as a harbor for the accumulation of mutations that can provide flexibility for conformational changes and responding to immune pressure.
MeSH term(s)	Computational Biology ; Conserved Sequence ; DNA-Directed DNA Polymerase/chemistry ; DNA-Directed DNA Polymerase/genetics ; DNA-Directed DNA Polymerase/metabolism ; Databases, Genetic ; Epitopes/genetics ; Evolution, Molecular ; Genome, Viral/genetics ; Hepatitis B virus/enzymology ; Hepatitis B virus/genetics ; Hepatitis B virus/metabolism ; Hepatitis B virus/physiology ; Humans ; Microbial Viability ; Models, Molecular ; Open Reading Frames/genetics ; Protein Structure, Secondary ; Protein Structure, Tertiary ; RNA-Directed DNA Polymerase/chemistry ; RNA-Directed DNA Polymerase/genetics ; RNA-Directed DNA Polymerase/metabolism ; Selection, Genetic ; Viral Proteins/chemistry ; Viral Proteins/genetics ; Viral Proteins/metabolism
Chemical Substances	Epitopes ; Viral Proteins ; RNA-Directed DNA Polymerase (EC 2.7.7.49) ; DNA-Directed DNA Polymerase (EC 2.7.7.7)
Language	English
Publishing date	2013-04-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0060098
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Atypical mitogen-activated protein kinase phosphatase implicated in regulating transition from pre-S-Phase asexual intraerythrocytic development of Plasmodium falciparum.

Balu, Bharath / Campbell, Christopher / Sedillo, Jennifer / Maher, Steven / Singh, Naresh / Thomas, Phaedra / Zhang, Min / Pance, Alena / Otto, Thomas D / Rayner, Julian C / Adams, John H

Eukaryotic cell

2013 Volume 12, Issue 9, Page(s) 1171–1178

Abstract: ... growth rate with delayed entry into the S/M phase of the cell cycle, which follows the stage of maximum PF3D7 ... as a regulator of pre-S-phase cell cycle progression in P. falciparum. ...

Abstract	Intraerythrocytic development of the human malaria parasite Plasmodium falciparum appears as a continuous flow through growth and proliferation. To develop a greater understanding of the critical regulatory events, we utilized piggyBac insertional mutagenesis to randomly disrupt genes. Screening a collection of piggyBac mutants for slow growth, we isolated the attenuated parasite C9, which carried a single insertion disrupting the open reading frame (ORF) of PF3D7_1305500. This gene encodes a protein structurally similar to a mitogen-activated protein kinase (MAPK) phosphatase, except for two notable characteristics that alter the signature motif of the dual-specificity phosphatase domain, suggesting that it may be a low-activity phosphatase or pseudophosphatase. C9 parasites demonstrated a significantly lower growth rate with delayed entry into the S/M phase of the cell cycle, which follows the stage of maximum PF3D7_1305500 expression in intact parasites. Genetic complementation with the full-length PF3D7_1305500 rescued the wild-type phenotype of C9, validating the importance of the putative protein phosphatase PF3D7_1305500 as a regulator of pre-S-phase cell cycle progression in P. falciparum.
MeSH term(s)	Amino Acid Motifs ; Amino Acid Sequence ; Catalytic Domain ; Ecthyma, Contagious ; Genes, Protozoan ; Merozoites/enzymology ; Merozoites/growth & development ; Mitogen-Activated Protein Kinase Phosphatases/chemistry ; Mitogen-Activated Protein Kinase Phosphatases/genetics ; Mitogen-Activated Protein Kinase Phosphatases/metabolism ; Mitosis ; Molecular Sequence Data ; Mutagenesis, Insertional ; Plasmodium falciparum/enzymology ; Plasmodium falciparum/genetics ; Plasmodium falciparum/growth & development ; Protozoan Proteins/chemistry ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; S Phase
Chemical Substances	Protozoan Proteins ; Mitogen-Activated Protein Kinase Phosphatases (EC 3.1.3.16)
Language	English
Publishing date	2013-06-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2077635-4
ISSN	1535-9786 ; 1535-9778
ISSN (online)	1535-9786
ISSN	1535-9778
DOI	10.1128/EC.00028-13
Database	MEDical Literature Analysis and Retrieval System OnLINE

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