Article ; Online: Development of an indirect ELISA system for diagnosis of porcine edema disease using recombinant modified Stx2e antigen.
Journal of applied microbiology
2024 Volume 135, Issue 2
Abstract: Aim: This study aimed to develop a sensitive and specific recombinant antigen protein indirect enzyme-linked immunosorbent assay (ELISA) kit to detect the Shiga toxin (Stx)-producing Escherichia coli (STEC) antibodies against porcine edema disease (ED).! ...
Abstract | Aim: This study aimed to develop a sensitive and specific recombinant antigen protein indirect enzyme-linked immunosorbent assay (ELISA) kit to detect the Shiga toxin (Stx)-producing Escherichia coli (STEC) antibodies against porcine edema disease (ED). Methods and results: The recombinant antigen was co-expressed with the STEC-derived Stx2e A2-fragment and Stx2e B protein in E. coli BL21(DE3) pLysS cells and purified using maltose-binding protein open columns. We used a Shiga-like toxin 2 antibody to test the specificity of the recombinant antigen in an indirect ELISA, which was detected in antigen-coated wells but not in uncoated wells. We tested the indirect ELISA system using samples from the STEC-immunized pig group, the commercial swine farm group, and healthy aborted fetal pleural effusion group; five and twenty samples, respectively, were positive for STEC in the former, whereas all three samples were negative for STEC in the latter. Conclusions: This newly developed indirect ELISA may be a specific method for diagnosing STEC infections in pigs. |
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MeSH term(s) | Swine ; Animals ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/veterinary ; Shiga-Toxigenic Escherichia coli ; Swine Diseases/diagnosis ; Enzyme-Linked Immunosorbent Assay/veterinary ; Enzyme-Linked Immunosorbent Assay/methods ; Edema |
Language | English |
Publishing date | 2024-01-29 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 1358023-1 |
ISSN | 1365-2672 ; 1364-5072 |
ISSN (online) | 1365-2672 |
ISSN | 1364-5072 |
DOI | 10.1093/jambio/lxae021 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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