LIVIVO - Search results -

Search results

Result 1 - 10 of total 20

Search options

Article: IRAP Endosomes Control Phagosomal Maturation in Dendritic Cells.

Weimershaus, Mirjana / Mauvais, François-Xavier / Evnouchidou, Irini / Lawand, Myriam / Saveanu, Loredana / van Endert, Peter

Frontiers in cell and developmental biology

2020 Volume 8, Page(s) 585713

Abstract: Dendritic cells (DCs) contribute to the immune surveillance by sampling their environment through phagocytosis and endocytosis. We have previously reported that, rapidly following uptake of extracellular antigen into phagosomes or endosomes in DCs, a ... ...

Abstract	Dendritic cells (DCs) contribute to the immune surveillance by sampling their environment through phagocytosis and endocytosis. We have previously reported that, rapidly following uptake of extracellular antigen into phagosomes or endosomes in DCs, a specialized population of storage endosomes marked by Rab14 and insulin-regulated aminopeptidase (IRAP) is recruited to the nascent antigen-containing compartment, thereby regulating its maturation and ultimately antigen cross-presentation to CD8
Language	English
Publishing date	2020-12-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2020.585713
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Unexpected lack of specificity of a rabbit polyclonal TAP-L (ABCB9) antibody.

van Endert, Peter / Lawand, Myriam

F1000Research

2015 Volume 4, Page(s) 125

Abstract: In this article, we describe the surprising non-specific reactivity in immunoblots of a rabbit polyclonal antibody (ref. Abcam 86222) expected to recognize the transporter associated with antigen processing like (TAP-L, ABCB9) protein. Although this ... ...

Abstract	In this article, we describe the surprising non-specific reactivity in immunoblots of a rabbit polyclonal antibody (ref. Abcam 86222) expected to recognize the transporter associated with antigen processing like (TAP-L, ABCB9) protein. Although this antibody, according to company documentation, recognizes a band with the expected molecular weight of 84 kDa in HeLa, 293T and mouse NIH3T3 whole-cell lysates, we found that this band is also present in immunoblots of TAP-L deficient bone marrow-derived dendritic cell (BMDC) whole-cell lysates in three independent replicates. We performed extensive verification by multiple PCR tests to confirm the complete absence of the ABCB9 gene in our TAP-L deficient mice. We conclude that the antibody tested cross-reacts with an unidentified protein present in TAP-L knockout cells, which coincidentally runs at the same molecular weight as TAP-L. These findings underline the pitfalls of antibody specificity testing in the absence of cells lacking expression of the target protein.
Language	English
Publishing date	2015
Publishing country	England
Document type	Journal Article
ZDB-ID	2699932-8
ISSN	2046-1402
ISSN	2046-1402
DOI	10.12688/f1000research.6535.1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Unexpected lack of specificity of a rabbit polyclonal TAP-L (ABCB9) antibody [v1; ref status

Peter van Endert / Myriam Lawand

F1000Research, Vol

indexed, http://f1000r.es/5ex]

2015 Volume 4

Abstract	In this article, we describe the surprising non-specific reactivity in immunoblots of a rabbit polyclonal antibody (ref. Abcam 86222) expected to recognize the transporter associated with antigen processing like (TAP-L, ABCB9) protein. Although this antibody, according to company documentation, recognizes a band with the expected molecular weight of 84 kDa in HeLa, 293T and mouse NIH3T3 whole-cell lysates, we found that this band is also present in immunoblots of TAP-L deficient bone marrow-derived dendritic cell (BMDC) whole-cell lysates in three independent replicates. We performed extensive verification by multiple PCR tests to confirm the complete absence of the ABCB9 gene in our TAP-L deficient mice. We conclude that the antibody tested cross-reacts with an unidentified protein present in TAP-L knockout cells, which coincidentally runs at the same molecular weight as TAP-L. These findings underline the pitfalls of antibody specificity testing in the absence of cells lacking expression of the target protein.
Keywords	Antigen Processing & Recognition ; Cell Signaling ; Stem Cells & Regeneration ; Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2015-05-01T00:00:00Z
Publisher	F1000 Research Ltd
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Key Features of Gamma-Delta T-Cell Subsets in Human Diseases and Their Immunotherapeutic Implications.

Lawand, Myriam / Déchanet-Merville, Julie / Dieu-Nosjean, Marie-Caroline

Frontiers in immunology

2017 Volume 8, Page(s) 761

Abstract: The unique features of gamma-delta (γδ) T cells, related to their antigen recognition capacity, their tissue tropism, and their cytotoxic function, make these cells ideal candidates that could be targeted to induce durable immunity in the context of ... ...

Abstract	The unique features of gamma-delta (γδ) T cells, related to their antigen recognition capacity, their tissue tropism, and their cytotoxic function, make these cells ideal candidates that could be targeted to induce durable immunity in the context of different pathologies. In this review, we focus on the main characteristics of human γδ T-cell subsets in diseases and the key mechanisms that could be explored to target these cells.
Language	English
Publishing date	2017
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2606827-8
ISSN	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2017.00761
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: IRAP Endosomes Control Phagosomal Maturation in Dendritic Cells

Mirjana Weimershaus / François-Xavier Mauvais / Irini Evnouchidou / Myriam Lawand / Loredana Saveanu / Peter van Endert

Frontiers in Cell and Developmental Biology, Vol

2020 Volume 8

Abstract	Dendritic cells (DCs) contribute to the immune surveillance by sampling their environment through phagocytosis and endocytosis. We have previously reported that, rapidly following uptake of extracellular antigen into phagosomes or endosomes in DCs, a specialized population of storage endosomes marked by Rab14 and insulin-regulated aminopeptidase (IRAP) is recruited to the nascent antigen-containing compartment, thereby regulating its maturation and ultimately antigen cross-presentation to CD8+ T lymphocytes. Here, using IRAP–/– DCs, we explored how IRAP modulates phagosome maturation dynamics and cross-presentation. We find that in the absence of IRAP, phagosomes acquire more rapidly late endosomal markers, are more degradative, and show increased microbicidal activity. We also report evidence for a role of vesicle trafficking from the endoplasmic reticulum (ER)–Golgi intermediate compartment to endosomes for the formation or stability of the IRAP compartment. Moreover, we dissect the dual role of IRAP as a trimming peptidase and a critical constituent of endosome stability. Experiments using a protease-dead IRAP mutant and pharmacological IRAP inhibition suggest that IRAP expression but not proteolytic activity is required for the formation of storage endosomes and for DC-typical phagosome maturation, whereas proteolysis is required for fully efficient cross-presentation. These findings identify IRAP as a key factor in cross-presentation, trimming peptides to fit the major histocompatibility complex class-I binding site while preventing their destruction through premature phagosome maturation.
Keywords	aminopeptidase ; oxytocinase ; phagosome maturation ; GLUT4-storage vesicle ; cross-presentation ; ERGIC ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Cytotoxic effect of

Haddoub, Carol / Rima, Mohamad / Heurtebise, Sandrine / Lawand, Myriam / Jundi, Dania / Sadek, Riyad / Amigorena, Sebastian / Fajloun, Ziad / Karam, Marc C

PeerJ

2020 Volume 8, Page(s) e9909

Abstract: Background: Montivipera bornmuelleri: Methods: The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection.: ... ...

Abstract	Background: Montivipera bornmuelleri Methods: The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results: The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of
Language	English
Publishing date	2020-10-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.9909
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations.

Devi-Marulkar, Priyanka / Kaplon, Hélène / Dieu-Nosjean, Marie-Caroline / Lawand, Myriam

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1845, Page(s) 189–204

Abstract: The tumor microenvironment is a complex network of interacting cells composed of immune and nonimmune cells. It has been reported that the composition of the immune contexture has a significant impact on tumor growth and patient survival in different ... ...

Abstract	The tumor microenvironment is a complex network of interacting cells composed of immune and nonimmune cells. It has been reported that the composition of the immune contexture has a significant impact on tumor growth and patient survival in different solid tumors. For instance, we and other groups have previously demonstrated that a strong infiltration of T-helper type 1 (Th1) or memory CD8
MeSH term(s)	Biomarkers ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/immunology ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/pathology ; Flow Cytometry/methods ; Gene Expression Profiling ; Humans ; Immunophenotyping ; Lung Neoplasms/genetics ; Lung Neoplasms/immunology ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Lymphocyte Subsets/immunology ; Lymphocyte Subsets/metabolism ; Lymphocytes, Tumor-Infiltrating/immunology ; Lymphocytes, Tumor-Infiltrating/metabolism ; Neoplasms/immunology ; Neoplasms/metabolism ; Neoplasms/pathology ; Tertiary Lymphoid Structures/genetics ; Tertiary Lymphoid Structures/immunology ; Tertiary Lymphoid Structures/metabolism ; Tertiary Lymphoid Structures/pathology ; Tumor Microenvironment/immunology
Chemical Substances	Biomarkers
Language	English
Publishing date	2018-08-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-8709-2_11
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Regulatory T cells infiltrate the tumor-induced tertiary lymphoïd structures and are associated with poor clinical outcome in NSCLC.

Devi-Marulkar, Priyanka / Fastenackels, Solène / Karapentiantz, Pierre / Goc, Jérémy / Germain, Claire / Kaplon, Hélène / Knockaert, Samantha / Olive, Daniel / Panouillot, Marylou / Validire, Pierre / Damotte, Diane / Alifano, Marco / Murris, Juliette / Katsahian, Sandrine / Lawand, Myriam / Dieu-Nosjean, Marie-Caroline

Communications biology

2022 Volume 5, Issue 1, Page(s) 1416

Abstract: On one hand, regulatory T cells (Tregs) play an immunosuppressive activity in most solid tumors but not all. On the other hand, the organization of tumor-infiltrating immune cells into tertiary lymphoid structures (TLS) is associated with long-term ... ...

Abstract	On one hand, regulatory T cells (Tregs) play an immunosuppressive activity in most solid tumors but not all. On the other hand, the organization of tumor-infiltrating immune cells into tertiary lymphoid structures (TLS) is associated with long-term survival in most cancers. Here, we investigated the role of Tregs in the context of Non-Small Cell Lung Cancer (NSCLC)-associated TLS. We observed that Tregs show a similar immune profile in TLS and non-TLS areas. Autologous tumor-infiltrating Tregs inhibit the proliferation and cytokine secretion of CD4
MeSH term(s)	Humans ; Carcinoma, Non-Small-Cell Lung/pathology ; T-Lymphocytes, Regulatory ; CD8-Positive T-Lymphocytes ; Lung Neoplasms/pathology ; Tertiary Lymphoid Structures/metabolism ; Tertiary Lymphoid Structures/pathology ; Lymphocytes, Tumor-Infiltrating
Language	English
Publishing date	2022-12-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-022-04356-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Contribution of resident and circulating precursors to tumor-infiltrating CD8

Gueguen, Paul / Metoikidou, Christina / Dupic, Thomas / Lawand, Myriam / Goudot, Christel / Baulande, Sylvain / Lameiras, Sonia / Lantz, Olivier / Girard, Nicolas / Seguin-Givelet, Agathe / Lefevre, Marine / Mora, Thierry / Walczak, Aleksandra M / Waterfall, Joshua J / Amigorena, Sebastian

Science immunology

2021 Volume 6, Issue 55

Abstract: Tumor-infiltrating lymphocytes (TILs), in general, and especially ... ...

Abstract	Tumor-infiltrating lymphocytes (TILs), in general, and especially CD8
MeSH term(s)	CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Carcinoma, Non-Small-Cell Lung/blood ; Carcinoma, Non-Small-Cell Lung/immunology ; Carcinoma, Non-Small-Cell Lung/pathology ; Carcinoma, Non-Small-Cell Lung/surgery ; Cell Differentiation/immunology ; Female ; Humans ; Lung/immunology ; Lung/pathology ; Lung/surgery ; Lung Neoplasms/blood ; Lung Neoplasms/immunology ; Lung Neoplasms/pathology ; Lung Neoplasms/surgery ; Lymphocytes, Tumor-Infiltrating/immunology ; Lymphocytes, Tumor-Infiltrating/metabolism ; Male ; Pneumonectomy ; Tumor Microenvironment/immunology
Language	English
Publishing date	2021-03-08
Publishing country	United States
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ISSN	2470-9468
ISSN (online)	2470-9468
DOI	10.1126/sciimmunol.abd5778
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines

Carol Haddoub / Mohamad Rima / Sandrine Heurtebise / Myriam Lawand / Dania Jundi / Riyad Sadek / Sebastian Amigorena / Ziad Fajloun / Marc C. Karam

PeerJ, Vol 8, p e

in vitro and in vivo studies

2020 Volume 9909

Abstract: Background Montivipera bornmuelleri’s venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating ...

Abstract	Background Montivipera bornmuelleri’s venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleri’s venom and its effect on tumor growth in vivo. Methods The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri’s venom in vitro which, however, does not translate to an anticancer action in vivo.
Keywords	Montivipera bornmuelleri ; B16 cells ; MCA cells ; Anti-cancer activity ; In vivo ; Medicine ; R
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	PeerJ Inc.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED