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Article: ESSAY. Wie unpolitisch ist Wissenschaft

Wyler, Emanuel

2021 Volume 28, Issue 7/8, Page(s) 16

Language	German
Document type	Article
ZDB-ID	1237282-1
ISSN	1612-8354
Database	Current Contents Medicine

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Article: Krankheitserreger mit Abwasser beobachten. Abwassermonitoring als zukünftiger Beitrag zur Prävention von Infektionskrankheiten

Wyler, Emanuel / Landthaler, Markus

KU-Gesundheitsmanagement

2023 Volume 92, Issue 10, Page(s) 61

Language	German
Document type	Article
ZDB-ID	2420760-3
ISSN	1867-9269
Database	Current Contents Medicine

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Book ; Online: Bulk and single-cell gene expression profiling of SARS-CoV-2 infected human cell lines identifies molecular targets for therapeutic intervention

Wyler Emanuel, Franke Vedran

2020

Abstract: Single cell RNA seq datasets used for analysis in the Bulk and single-cell gene expression profiling of SARS-CoV-2 infected human cell lines identifies molecular targets for therapeutic ... ...

Abstract	Single cell RNA seq datasets used for analysis in the Bulk and single-cell gene expression profiling of SARS-CoV-2 infected human cell lines identifies molecular targets for therapeutic intervention
Keywords	SARS-CoV ; SARS-CoV2 ; Single Cell Sequencing ; dropseq ; virus ; covid19
Publishing date	2020-09-15
Publishing country	eu
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: SARS-CoV-2 in humans

Marco Binder / Emanuel Wyler

Berliner und Münchener Tierärztliche Wochenschrift (2021)

2021

Abstract: The novel coronavirus SARS-CoV-2 became pandemic at the beginning of 2020, and caused about 80 million cases and more than 1.8 million deaths by the end of the year. As its relatives MERS- and SARS-CoV, but in contrast to the four human coronaviruses ... ...

Abstract	The novel coronavirus SARS-CoV-2 became pandemic at the beginning of 2020, and caused about 80 million cases and more than 1.8 million deaths by the end of the year. As its relatives MERS- and SARS-CoV, but in contrast to the four human coronaviruses circulating worldwide, SARS-CoV-2 in a sizeable fraction of cases leads to a severe and potentially life-threatening disease, called COVID-19. Since in addition this virus is very contagious, particularly prior to onset or in absence of symptoms, and pre-existing immunity appears to be largely absent or at least of very little relevance, it is spreading rapidly in the population. A hallmark of COVID-19 is an at least partially detrimental immune response that not only can lead to serious lung damage, but may also damage organs outside the respiratory tract such as the heart and kidneys. This review summarizes current knowledge about the virus and the disease it causes, and outlines open questions in the different research fields.
Keywords	sars-cov-2 ; covid-19 ; molecular biology ; transmission ; epidemiol-ogy ; pathogenesis ; immunity ; Veterinary medicine ; SF600-1100
Language	German
Publishing date	2021-04-01T00:00:00Z
Publisher	Schlütersche Fachmedien GmbH
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Thesis: Form- und Stiluntersuchungen zum ersten Satz der Klaviersonaten Carl Philipp Emanuel Bachs

Wyler, Robert / Bach, Carl Philipp Emanuel

1960

Author's details	von Robert Wyler
Language	German
Size	160 S., Notenbeisp., 8°
Publisher	Schüler
Publishing place	Biel
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Phil. Fak., Diss.--Zürich, 1960
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Grainyhead-like 2 Deficiency and Kidney Cyst Growth in a Mouse Model.

Yurtdas, Zeliha Yesim / Kilic, Ergin / Boor, Peter / Wyler, Emanuel / Landthaler, Markus / Jung, Klaus / Schmidt-Ott, Kai M

Journal of the American Society of Nephrology : JASN

2024

Abstract: Background: The transcription factor Grainyhead-like 2 (GRHL2) plays a crucial role in maintaining the epithelial barrier properties of the renal collecting duct and is essential for osmoregulation. We noticed a reduction in GRHL2 expression in cysts ... ...

Abstract	Background: The transcription factor Grainyhead-like 2 (GRHL2) plays a crucial role in maintaining the epithelial barrier properties of the renal collecting duct and is essential for osmoregulation. We noticed a reduction in GRHL2 expression in cysts derived from the collecting ducts in kidneys affected by Autosomal Dominant Polycystic Kidney Disease (ADPKD). However, the specific role of GRHL2 in cystic kidney disease remains unknown. Methods: The functional role of the transcription factor Grhl2 in the context of cystic kidney disease was examined through analysis of its expression pattern in patient samples with ADPKD and generating a transgenic cystic kidney disease (TCKD) mouse model by overexpressing the human proto-oncogene c-MYC in kidney collecting ducts. Next, TCKD mice bred with collecting duct-specific Grhl2 knockout mice (Grhl2KO). The resulting TCKD-Grhl2KO mice and their littermates were examined by various types of histological and biochemical assays and gene profiling analysis via RNA-seq. Results: A comprehensive examination of kidney samples from patients with ADPKD revealed GRHL2 downregulation in collecting duct-derived cyst epithelia. Comparative analysis of TCKD and TCKD-Grhl2KO mice exhibited that the collecting duct-specific deletion of Grhl2 resulted in markedly aggravated cyst growth, worsened kidney dysfunction, and shortened life span. Furthermore, transcriptomic analyses indicated sequential downregulation of kidney epithelial cyst development regulators (Frem2, Muc1, Cdkn2c, Pkd2, and Tsc1) during cyst progression in kidneys of TCKD-Grhl2KO mice which included presumed direct Grhl2 target genes. Conclusions: These results suggest GRHL2 as a potential progression modifier, especially for cysts originating from collecting ducts.
Language	English
Publishing date	2024-04-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	1085942-1
ISSN	1533-3450 ; 1046-6673
ISSN (online)	1533-3450
ISSN	1046-6673
DOI	10.1681/ASN.0000000000000353
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In Vitro Kinase-to-Phosphosite Database (iKiP-DB) Predicts Kinase Activity in Phosphoproteomic Datasets.

Mari, Tommaso / Mösbauer, Kirstin / Wyler, Emanuel / Landthaler, Markus / Drosten, Christian / Selbach, Matthias

Journal of proteome research

2022 Volume 21, Issue 6, Page(s) 1575–1587

Abstract: Phosphoproteomics routinely quantifies changes in the levels of thousands of phosphorylation sites, but functional analysis of such data remains a major challenge. While databases like PhosphoSitePlus contain information about many phosphorylation sites, ...

Abstract	Phosphoproteomics routinely quantifies changes in the levels of thousands of phosphorylation sites, but functional analysis of such data remains a major challenge. While databases like PhosphoSitePlus contain information about many phosphorylation sites, the vast majority of known sites is not assigned to any protein kinase. Assigning changes in the phosphoproteome to the activity of individual kinases therefore remains a key challenge. A recent large-scale study systematically identified in vitro substrates for most human protein kinases. Here, we reprocessed and filtered these data to generate an
MeSH term(s)	COVID-19 ; Humans ; Phosphoproteins/metabolism ; Phosphorylation ; Protein Kinases/genetics ; Protein Kinases/metabolism ; SARS-CoV-2
Chemical Substances	Phosphoproteins ; Protein Kinases (EC 2.7.-)
Language	English
Publishing date	2022-05-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.2c00198
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture.

Dasgupta, Sandipan / Dayagi, Daniella Y / Haimovich, Gal / Wyler, Emanuel / Olender, Tsviya / Singer, Robert H / Landthaler, Markus / Gerst, Jeffrey E

eLife

2023 Volume 12

Abstract: Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the 'transferome') is unknown. Here, we analyzed the ... ...

Abstract	Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the 'transferome') is unknown. Here, we analyzed the transferome in an in vitro human-mouse cell co-culture model using RNA-sequencing. We found that mRNA transfer is non-selective, prevalent across the human transcriptome, and that the amount of transfer to mouse embryonic fibroblasts (MEFs) strongly correlates with the endogenous level of gene expression in donor human breast cancer cells. Typically,<1% of endogenous mRNAs undergo transfer. Non-selective, expression-dependent RNA transfer was further validated using synthetic reporters. RNA transfer appears contact-dependent via TNTs, as exemplified for several mRNAs. Notably, significant differential changes in the native MEF transcriptome were observed in response to co-culture, including the upregulation of multiple cancer and cancer-associated fibroblast-related genes and pathways. Together, these results lead us to suggest that TNT-mediated RNA transfer could be a phenomenon of physiological importance under both normal and pathogenic conditions.
MeSH term(s)	Humans ; Mice ; Animals ; RNA, Long Noncoding/genetics ; RNA, Messenger/genetics ; Fibroblasts ; Nanotubes ; Cell Culture Techniques ; Cell Communication/physiology ; Mammals
Chemical Substances	Tunneling Nanotubes ; RNA, Long Noncoding ; RNA, Messenger
Language	English
Publishing date	2023-05-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.83584
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The Interactive Complex between Cytomegalovirus Kinase vCDK/pUL97 and Host Factors CDK7-Cyclin H Determines Individual Patterns of Transcription in Infected Cells.

Schütz, Martin / Cordsmeier, Arne / Wangen, Christina / Horn, Anselm H C / Wyler, Emanuel / Ensser, Armin / Sticht, Heinrich / Marschall, Manfred

International journal of molecular sciences

2023 Volume 24, Issue 24

Abstract: The infection of human cytomegalovirus (HCMV) is strongly determined by the host-cell interaction in a way that the efficiency of HCMV lytic replication is dependent on the regulatory interplay between viral and cellular proteins. In particular, the ... ...

Abstract	The infection of human cytomegalovirus (HCMV) is strongly determined by the host-cell interaction in a way that the efficiency of HCMV lytic replication is dependent on the regulatory interplay between viral and cellular proteins. In particular, the activities of protein kinases, such as cyclin-dependent kinases (CDKs) and the viral CDK ortholog (vCDK/pUL97), play an important role in both viral reproduction and virus-host interaction. Very recently, we reported on the complexes formed between vCDK/pUL97, human cyclin H, and CDK7. Major hallmarks of this interplay are the interaction between cyclin H and vCDK/pUL97, which is consistently detectable across various conditions and host cell types of infection, the decrease or increase in pUL97 kinase activity resulting from cyclin H knock-down or elevated levels, respectively, and significant trans-stimulation of human CDK7 activity by pUL97 in vitro. Due to the fact that even a ternary complex of vCDK/pUL97-cyclin H-CDK7 can be detected by coimmunoprecipitation and visualized by bioinformatic structural modeling, we postulated a putative impact of the respective kinase activities on the patterns of transcription in HCMV-infected cells. Here, we undertook a first vCDK/pUL97-specific transcriptomic analysis, which combined conditions of fully lytic HCMV replication with those under specific vCDK/pUL97 or CDK7 drug-mediated inhibition or transient cyclin H knockout. The novel results were further strengthened using bioinformatic modeling of the involved multi-protein complexes. Our data underline the importance of these kinase activities for the C-terminal domain (CTD) phosphorylation-driven activation of host RNA polymerase in HCMV-infected cells. The impact of the individual experimental conditions on differentially expressed gene profiles is described in detail and discussed.
MeSH term(s)	Humans ; Cyclins/metabolism ; Cytomegalovirus/genetics ; Cyclin H/metabolism ; Cyclin-Dependent Kinases/genetics ; Cyclin-Dependent Kinases/metabolism ; Phosphorylation ; Herpesviridae Infections
Chemical Substances	Cyclins ; Cyclin H ; Cyclin-Dependent Kinases (EC 2.7.11.22)
Language	English
Publishing date	2023-12-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242417421
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture

Sandipan Dasgupta / Daniella Y Dayagi / Gal Haimovich / Emanuel Wyler / Tsviya Olender / Robert H Singer / Markus Landthaler / Jeffrey E Gerst

eLife, Vol

2023 Volume 12

Abstract: Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the ‘transferome’) is unknown. Here, we analyzed the ... ...

Abstract	Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the ‘transferome’) is unknown. Here, we analyzed the transferome in an in vitro human-mouse cell co-culture model using RNA-sequencing. We found that mRNA transfer is non-selective, prevalent across the human transcriptome, and that the amount of transfer to mouse embryonic fibroblasts (MEFs) strongly correlates with the endogenous level of gene expression in donor human breast cancer cells. Typically,<1% of endogenous mRNAs undergo transfer. Non-selective, expression-dependent RNA transfer was further validated using synthetic reporters. RNA transfer appears contact-dependent via TNTs, as exemplified for several mRNAs. Notably, significant differential changes in the native MEF transcriptome were observed in response to co-culture, including the upregulation of multiple cancer and cancer-associated fibroblast-related genes and pathways. Together, these results lead us to suggest that TNT-mediated RNA transfer could be a phenomenon of physiological importance under both normal and pathogenic conditions.
Keywords	cell-cell RNA transfer ; tunneling nanotubes ; RNA trafficking ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	eLife Sciences Publications Ltd
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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