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  1. Article ; Online: Visualisation of microalgal lipid bodies through electron microscopy.

    Verwee, Ellen / Van de Walle, Davy / De Bruyne, Michiel / Mienis, Esther / Sekulic, Mirna / Chaerle, Peter / Vyverman, Wim / Foubert, Imogen / Dewettinck, Koen

    Journal of microscopy

    2024  Volume 293, Issue 2, Page(s) 118–131

    Abstract: In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were ... ...

    Abstract In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were harvested in both the mid-exponential and early stationary growth phase. Two different cryo-SEM cutting methods were compared: cryo-planing and freeze-fracturing. The results showed that, despite the longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear detection of lipid bodies and is preferable to cryo-SEM. Using freeze-fracturing, lipid bodies were rarely detected. This was only feasible if crystalline layers in the internal structure, most likely related to sterol esters or di-saturated triacylglycerols, were revealed. Furthermore, lipid bodies could not be detected using cryo-planing. Cryo-SEM is also not the preferred technique to recognise other organelles besides lipid bodies, yet it did reveal chloroplasts in both species and filament-containing organelles in cryo-planed Nannochloropsis oculata samples.
    MeSH term(s) Microalgae ; Lipid Droplets ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Cryoelectron Microscopy/methods
    Language English
    Publishing date 2024-01-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 219263-9
    ISSN 1365-2818 ; 0022-2720
    ISSN (online) 1365-2818
    ISSN 0022-2720
    DOI 10.1111/jmi.13259
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 838: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article ; Online: Key signalling nodes in mammary gland development and cancer. The Snail1-Twist1 conspiracy in malignant breast cancer progression.

    Foubert, Ellen / De Craene, Bram / Berx, Geert

    Breast cancer research : BCR

    2010  Volume 12, Issue 3, Page(s) 206

    Abstract: Breast cancer is the most common cancer among women, and despite significant advances in diagnosing and treating it, metastatic spread of cancer cells results in a high mortality rate. Epithelial-to-mesenchymal transition (EMT) is an embryonic program in ...

    Abstract Breast cancer is the most common cancer among women, and despite significant advances in diagnosing and treating it, metastatic spread of cancer cells results in a high mortality rate. Epithelial-to-mesenchymal transition (EMT) is an embryonic program in which epithelial cells lose their characteristics and gain mesenchymal features. Therefore, EMT might play a very important role during malignant tumour progression. In this review we summarise recent advances in breast cancer research with a particular focus on the transcription factors Snail1 and Twist1. Besides discussing the role of EMT in normal mammary gland development, we describe regulatory mechanisms involving newly discovered upstream regulators and microRNAs, the association of EMT with breast cancer stem cells, and the involvement of the tumour microenvironment in breast cancer progression.
    MeSH term(s) Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Disease Progression ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Female ; Humans ; Mesoderm/metabolism ; Mesoderm/pathology ; Nuclear Proteins/metabolism ; Snail Family Transcription Factors ; Transcription Factors/metabolism ; Twist-Related Protein 1/metabolism
    Chemical Substances Nuclear Proteins ; SNAI1 protein, human ; Snail Family Transcription Factors ; TWIST1 protein, human ; Transcription Factors ; Twist-Related Protein 1
    Language English
    Publishing date 2010-06-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2015059-3
    ISSN 1465-542X ; 1465-5411
    ISSN (online) 1465-542X
    ISSN 1465-5411
    DOI 10.1186/bcr2585
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5494: Show issues Location:
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  3. Article: Saponin production is not qualitatively changed upon callus regeneration in the medicinal shrub Maesa perlarius

    Faizal, Ahmad / Foubert, Kenn / Lambert, Ellen / De Storme, Nico / Claeys, Magda / Apers, Sandra / Geelen, Danny

    Plant growth regulation. 2013 May, v. 70, no. 1

    2013  

    Abstract: Maesa perlarius is a medicinal plant that produces maesabalides, which possess selective and strong anti-leishmania activity. In this study, M. perlarius plants were regenerated from leaf-derived calli. Shoots were induced in Murashige and Skoog medium ... ...

    Abstract Maesa perlarius is a medicinal plant that produces maesabalides, which possess selective and strong anti-leishmania activity. In this study, M. perlarius plants were regenerated from leaf-derived calli. Shoots were induced in Murashige and Skoog medium in the presence of thidiazuron in combination with α-naphthalene acetic acid. In contrast to seed-derived plants, callus-derived regenerants were tetraploid showing typical characteristics of higher ploidy phenotypes. We assessed the impact of indirect plant regeneration and associated increase in ploidy on the production of saponin by means of LC–MS analysis. Tetraploid M. perlarius produce a saponin profile, which was not significantly different from seed grown plants. Based on this study, we concluded that saponin production in M. perlarius is not qualitatively changed by a genome-doubling event.
    Keywords Maesa ; acetic acid ; callus ; medicinal plants ; phenotype ; seeds ; shoots ; shrubs ; tetraploidy
    Language English
    Dates of publication 2013-05
    Size p. 39-48.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 843025-1
    ISSN 1573-5087 ; 0167-6903
    ISSN (online) 1573-5087
    ISSN 0167-6903
    DOI 10.1007/s10725-012-9776-1
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  4. Article: Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods.

    Rajkovic, Andreja / El-Moualij, Benaissa / Uyttendaele, Mieke / Brolet, Philippe / Zorzi, Willy / Heinen, Ernst / Foubert, Ellen / Debevere, Johan

    Applied and environmental microbiology

    2006  Volume 72, Issue 10, Page(s) 6593–6599

    Abstract: A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of ... ...

    Abstract A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared to an in-house enzyme-linked immunosorbent assay (ELISA) using the same couple of capture-detection antibodies and to commercial kits for detection of S. aureus enterotoxins (SE). The iqPCR was approximately 1,000 times more sensitive (<10 pg ml(-1)) than the in-house ELISA and had a dynamic range of approximately 10 pg ml(-1) to approximately 30,000 pg ml(-1). iqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods. No cross-reactivity with SE other than SEB was observed. Application of iqPCR for detection of SEB in cultures of S. aureus revealed the onset of SEB production after 4 h of incubation at 22, 37, and 42 degrees C, which was in the first half of the exponential growth phase. The total amounts of SEB produced by the two strains tested were larger at 42 degrees C than at 37 degrees C and were strain dependent.
    MeSH term(s) Bacterial Toxins/analysis ; DNA Primers ; DNA Probes ; Enterotoxins/analysis ; Enzyme-Linked Immunosorbent Assay ; Food Analysis ; Food Microbiology ; Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Staphylococcal Food Poisoning ; Staphylococcus aureus/chemistry ; Staphylococcus aureus/metabolism
    Chemical Substances Bacterial Toxins ; DNA Primers ; DNA Probes ; Enterotoxins
    Language English
    Publishing date 2006-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.03068-05
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 201: Show issues Location:
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  5. Article: Immunoquantitative Real-Time PCR for Detection and Quantification of Staphylococcus aureus Enterotoxin B in Foods

    Rajkovic, Andreja / Moualij, Benaissa El / Uyttendaele, Mieke / Brolet, Philippe / Zorzi, Willy / Heinen, Ernst / Foubert, Ellen / Debevere, Johan

    Applied and environmental microbiology AEM. 2006 Oct., v. 72, no. 10

    2006  

    Abstract: A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of ... ...

    Abstract A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared to an in-house enzyme-linked immunosorbent assay (ELISA) using the same couple of capture-detection antibodies and to commercial kits for detection of S. aureus enterotoxins (SE). The iqPCR was approximately 1,000 times more sensitive (<10 pg ml-1) than the in-house ELISA and had a dynamic range of approximately 10 pg ml-1 to approximately 30,000 pg ml-1. iqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods. No cross-reactivity with SE other than SEB was observed. Application of iqPCR for detection of SEB in cultures of S. aureus revealed the onset of SEB production after 4 h of incubation at 22, 37, and 42°C, which was in the first half of the exponential growth phase. The total amounts of SEB produced by the two strains tested were larger at 42°C than at 37°C and were strain dependent.
    Keywords Staphylococcus aureus ; food pathogens ; food contamination ; bacterial contamination ; enterotoxins ; food microbiology ; polymerase chain reaction ; immunoassays ; DNA probes ; cultured cells
    Language English
    Dates of publication 2006-10
    Size p. 6593-6599.
    Publishing place American Society for Microbiology
    Document type Article
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2498: Show issues

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