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  1. Article: Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection.

    Strömer, Annabelle / Rose, Ruben / Grobe, Olaf / Neumann, Franziska / Fickenscher, Helmut / Lorentz, Thomas / Krumbholz, Andi

    Microorganisms

    2020  Volume 8, Issue 10

    Abstract: Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins ... ...

    Abstract Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of
    Keywords covid19
    Language English
    Publishing date 2020-10-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms8101572
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection

    Strömer, Annabelle / Rose, Ruben / Grobe, Olaf / Neumann, Franziska / Fickenscher, Helmut / Lorentz, Thomas / Krumbholz, Andi

    Microorganisms. 2020 Oct. 13, v. 8, no. 10

    2020  

    Abstract: Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection. Two ... ...

    Abstract Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection. Two immunoassays were selected out of eight IgG or total antibody tests by comparing their specificities and sensitivities. Sensitivities were calculated with convalescent sera from 26 PCR-confirmed cases, of which 76.9% had neutralizing antibodies (>1:10). Stored sera collected during the summer 2018 (N = 50) and winter seasons 2018/2019 (N = 50) were included to demonstrate the test specificities. IgG kinetics, avidities, and virus-neutralizing capacities were recorded over up to 165 days in eleven patients and five individuals from routine diagnostics. Sensitivities, specificities, and diagnostic accuracies ranged between 80.8–96.3%, 96.0–100%, and 93.7–99.2%, respectively. Nearly all results were confirmed with two different SARS-CoV-2-specific immunoblots. Six (54.4%) patients exhibited stable N-specific IgG indices over 120 days and longer; three of them developed IgG of high avidity. The S-specific IgG response was stable in ten (91.0%) patients, and eight (72.7%) had neutralizing antibodies. However, the titers were relatively low, suggesting that sustained humoral immunity is uncertain, especially after outpatient SARS-CoV-2 infection.
    Keywords Severe acute respiratory syndrome coronavirus ; diagnostic techniques ; humoral immunity ; immunoassays ; immunoglobulin G ; infection ; kinetics ; microorganisms ; neutralizing antibodies ; patients ; summer ; winter
    Language English
    Dates of publication 2020-1013
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms8101572
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen.

    Strömer, Annabelle / Rose, Ruben / Schäfer, Miriam / Schön, Frieda / Vollersen, Anna / Lorentz, Thomas / Fickenscher, Helmut / Krumbholz, Andi

    Microorganisms

    2020  Volume 9, Issue 1

    Abstract: The rapid detection of infections caused by ... ...

    Abstract The rapid detection of infections caused by the
    Language English
    Publishing date 2020-12-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9010058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen

    Strömer, Annabelle / Rose, Ruben / Schäfer, Miriam / Schön, Frieda / Vollersen, Anna / Lorentz, Thomas / Fickenscher, Helmut / Krumbholz, Andi

    Microorganisms. 2020 Dec. 28, v. 9, no. 1

    2020  

    Abstract: The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 ... ...

    Abstract The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 NADAL® COVID-19 Ag) was compared to a laboratory-developed triplex real-time polymerase chain reaction (RT-PCR) designed for the detection of viral nucleoprotein gene and two control targets. This RT-PCR served as a reference to investigate POCT sensitivity by re-testing upper respiratory tract (URT) samples (n = 124) exhibiting different SARS-CoV-2 loads in terms of RT-PCR threshold cycle (Ct) values. The optical intensities of the antigen bands were compared to the Ct values of the RT-PCR. The infectivity of various virus loads was estimated by inoculating Vero cells with URT samples (n = 64, Ct 17-34). POCT sensitivity varied from 100% (Ct < 25) to 73.1% (Ct ≤ 30); higher SARS-CoV-2 loads correlated with higher band intensities. All samples with a Ct > 30 were negative; among SARS-CoV-2 free samples (n = 10) no false-positives were detected. A head-to-head comparison with another POCT (Abbott, Panbio™ COVID-19 Ag Rapid Test) yielded similar results. Isolation of SARS-CoV-2 in cell-culture was successful up to a Ct value of 29. The POCT reliably detects high SARS-CoV-2 loads and rapidly identifies infectious individuals.
    Keywords Coronavirus infections ; Severe acute respiratory syndrome coronavirus ; antigens ; cell culture ; cells ; detection ; genes ; microorganisms ; nucleoproteins ; pandemic ; pathogenicity ; quantitative polymerase chain reaction ; rapid methods ; respiratory system ; sampling ; viral load
    Language English
    Dates of publication 2020-1228
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9010058
    Database NAL-Catalogue (AGRICOLA)

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    Order via subito

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  5. Article ; Online: Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection

    Annabelle Strömer / Ruben Rose / Olaf Grobe / Franziska Neumann / Helmut Fickenscher / Thomas Lorentz / Andi Krumbholz

    Microorganisms, Vol 8, Iss 1572, p

    2020  Volume 1572

    Abstract: Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection. Two ...

    Abstract Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection. Two immunoassays were selected out of eight IgG or total antibody tests by comparing their specificities and sensitivities. Sensitivities were calculated with convalescent sera from 26 PCR-confirmed cases, of which 76.9% had neutralizing antibodies (>1:10). Stored sera collected during the summer 2018 (N = 50) and winter seasons 2018/2019 (N = 50) were included to demonstrate the test specificities. IgG kinetics, avidities, and virus-neutralizing capacities were recorded over up to 165 days in eleven patients and five individuals from routine diagnostics. Sensitivities, specificities, and diagnostic accuracies ranged between 80.8–96.3%, 96.0–100%, and 93.7–99.2%, respectively. Nearly all results were confirmed with two different SARS-CoV-2-specific immunoblots. Six (54.4%) patients exhibited stable N-specific IgG indices over 120 days and longer; three of them developed IgG of high avidity. The S-specific IgG response was stable in ten (91.0%) patients, and eight (72.7%) had neutralizing antibodies. However, the titers were relatively low, suggesting that sustained humoral immunity is uncertain, especially after outpatient SARS-CoV-2 infection.
    Keywords COVID-19 ; humoral immunity ; test performance ; neutralization ; follow-up ; titer decrease ; Biology (General) ; QH301-705.5 ; covid19
    Subject code 616
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  6. Article ; Online: Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen

    Annabelle Strömer / Ruben Rose / Miriam Schäfer / Frieda Schön / Anna Vollersen / Thomas Lorentz / Helmut Fickenscher / Andi Krumbholz

    Microorganisms, Vol 9, Iss 58, p

    2021  Volume 58

    Abstract: The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 ... ...

    Abstract The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 NADAL ® COVID-19 Ag) was compared to a laboratory-developed triplex real-time polymerase chain reaction (RT-PCR) designed for the detection of viral nucleoprotein gene and two control targets. This RT-PCR served as a reference to investigate POCT sensitivity by re-testing upper respiratory tract (URT) samples ( n = 124) exhibiting different SARS-CoV-2 loads in terms of RT-PCR threshold cycle (Ct) values. The optical intensities of the antigen bands were compared to the Ct values of the RT-PCR. The infectivity of various virus loads was estimated by inoculating Vero cells with URT samples ( n = 64, Ct 17-34). POCT sensitivity varied from 100% (Ct < 25) to 73.1% (Ct ≤ 30); higher SARS-CoV-2 loads correlated with higher band intensities. All samples with a Ct > 30 were negative; among SARS-CoV-2 free samples ( n = 10) no false-positives were detected. A head-to-head comparison with another POCT (Abbott, Panbio™ COVID-19 Ag Rapid Test) yielded similar results. Isolation of SARS-CoV-2 in cell-culture was successful up to a Ct value of 29. The POCT reliably detects high SARS-CoV-2 loads and rapidly identifies infectious individuals.
    Keywords COVID-19 ; diagnostic ; PCR ; antigen ; POCT ; comparison ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection

    Strömer, Annabelle / Rose, Ruben / Grobe, Olaf / Neumann, Franziska / Fickenscher, Helmut / Lorentz, Thomas / Krumbholz, Andi

    Microorganisms

    Abstract: Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection Two ... ...

    Abstract Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of severe acute respiratory syndrome coronavirus type2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection Two immunoassays were selected out of eight IgG or total antibody tests by comparing their specificities and sensitivities Sensitivities were calculated with convalescent sera from 26 PCR-confirmed cases, of which 76 9% had neutralizing antibodies (>1:10) Stored sera collected during the summer 2018 (N = 50) and winter seasons 2018/2019 (N = 50) were included to demonstrate the test specificities IgG kinetics, avidities, and virus-neutralizing capacities were recorded over up to 165 days in eleven patients and five individuals from routine diagnostics Sensitivities, specificities, and diagnostic accuracies ranged between 80 8–96 3%, 96 0–100%, and 93 7–99 2%, respectively Nearly all results were confirmed with two different SARS-CoV-2-specific immunoblots Six (54 4%) patients exhibited stable N-specific IgG indices over 120 days and longer;three of them developed IgG of high avidity The S-specific IgG response was stable in ten (91 0%) patients, and eight (72 7%) had neutralizing antibodies However, the titers were relatively low, suggesting that sustained humoral immunity is uncertain, especially after outpatient SARS-CoV-2 infection
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #847649
    Database COVID19

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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