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Article ; Online: Ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix improves rabbit temporomandibular joint osteoarthrosis.

Guo, Yanwei / Tian, Tingyu / Yang, Shimao / Cai, Yuping

Biotechnology & genetic engineering reviews

2023 , Page(s) 1–22

Abstract: Objective: To investigate whether and how ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix can improve rabbit temporomandibular joint osteoarthrosis.: Method: Isolate and culture adipose stem cells, measure the activity of ... ...

Abstract	Objective: To investigate whether and how ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix can improve rabbit temporomandibular joint osteoarthrosis. Method: Isolate and culture adipose stem cells, measure the activity of differentiated chondrocytes by MTT assay and expression of type II collagen in these cells by immunohistochemistry, in order to evaluate the effect of ginsenoside Rg1 on adipose stem cell proliferation and differentiation into chondrocytes.32 New Zealand white rabbits were randomly divided into four groups: blank group, model group, control group and experimental group, 8 in each group. Osteoarthritis model was established by intra-articular injection of papain. Two weeks after successful model building, medication was given for the rabbits in control group and experimental group. 0.6 mL ginsenoside Rg1/ ADSCs suspension was injected into superior joint space for the rabbits in control group, once a week; 0.6 mL ginsenoside Rg1/ ADSCs complex was injected for the rabbits in experimental group, once a week. Results: Ginsenoside Rg1 can promote ADSCs-derived chondrocytes' activity and expression of type II collagen. Scanning electron microscopy histology images showed cartilage lesions of the experimental group was significantly improved in comparison with control group. Conclusion: Ginsenoside Rg1 can promote ADSCs differentiate into chondrocytes, and Ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix can significantly improve rabbit temporomandibular joint osteoarthrosis.
Language	English
Publishing date	2023-03-09
Publishing country	England
Document type	Journal Article
ZDB-ID	49457-4
ISSN	2046-5556 ; 0264-8725
ISSN (online)	2046-5556
ISSN	0264-8725
DOI	10.1080/02648725.2023.2183575
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Article ; Online: Compositional and spectroscopic analysis of dissolved organic matter samples from Everglades periphyton and water.

Anjuman, Afia / Xiang, Yuping / Liu, Guangliang / Cai, Yong

Environmental science and pollution research international

2023 Volume 30, Issue 48, Page(s) 106502–106513

Abstract: Periphyton is a ubiquitous niche in aquatic environments and can be a significant source of dissolved organic matter (DOM) production and leaching, especially in such environment as the Everglades, a slow-water flow wetland in Florida, USA. We employed ... ...

Abstract	Periphyton is a ubiquitous niche in aquatic environments and can be a significant source of dissolved organic matter (DOM) production and leaching, especially in such environment as the Everglades, a slow-water flow wetland in Florida, USA. We employed an array of methods, including compositional analysis, 3-dimensional excitation emission matrix (3-D EEM) fluorescence spectroscopy, and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, to perform quantitative and qualitative analyses on the DOM produced by periphyton and DOM in surrounding surface water and periphyton overlying water for comparison purposes. Higher dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) contents in periphyton pore water than surface water and periphyton overlying water indicated the remarkable contribution from periphyton-produced DOM. Higher total protein, carbohydrate, and thiol contents in periphyton pore water than in surface water and periphyton overlying water underscored the possibility of periphyton pore water DOM leached from periphyton. These results agreed with 3-D EEM and ATR-FTIR analyses that showed the prevalence of possible microbial source of periphyton pore water DOM as indicated by higher fluorescence index (FI) than surface water and periphyton overlying water. Similarly, the size-fractionated DOM from surface water demonstrated terrestrial sources, and periphyton pore water demonstrated microbial sources regardless of their differences in size based on their FI values. The types of periphyton affect the production and composition of DOM, as evidenced by higher total protein, carbohydrate, and chlorophyll-a (Chl-a) contents in floating mat on the water surface than in epiphyton attached to submerged phytoplankton, probably because the former is photo-synthetically more productive than the latter due to different light availability. This study provided fundamental information on periphyton DOM that is essential for further investigating its role in carbon cycle and its biogeochemistry.
MeSH term(s)	Water ; Dissolved Organic Matter ; Periphyton ; Organic Chemicals/analysis ; Spectrometry, Fluorescence/methods ; Carbohydrates
Chemical Substances	Water (059QF0KO0R) ; Dissolved Organic Matter ; Organic Chemicals ; Carbohydrates
Language	English
Publishing date	2023-09-20
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-29461-1
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Article: A mixed virtual element method for Biot's consolidation model.

Wang, Feng / Cai, Mingchao / Wang, Gang / Zeng, Yuping

Computers & mathematics with applications (Oxford, England : 1987)

2022 Volume 126, Page(s) 31–42

Abstract: In this paper, we shall present a weak virtual element method for the standard three field poroelasticity problem on polytopal meshes. The flux velocity and pressure are approximated by the low ... ...

Abstract	In this paper, we shall present a weak virtual element method for the standard three field poroelasticity problem on polytopal meshes. The flux velocity and pressure are approximated by the low order
Language	English
Publishing date	2022-09-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2004251-6
ISSN	1873-7668 ; 0898-1221
ISSN (online)	1873-7668
ISSN	0898-1221
DOI	10.1016/j.camwa.2022.09.005
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Article ; Online: Transplantation of beige adipose organoids fabricated using adipose acellular matrix hydrogel improves metabolic dysfunction in high-fat diet-induced obesity and type 2 diabetes mice.

Quan, Yuping / Li, Jian / Cai, Junrong / Liao, Yunjun / Zhang, Yuteng / Lu, Feng

Journal of cellular physiology

2024 Volume 239, Issue 4, Page(s) e31191

Abstract: Transplantation of brown adipose tissue (BAT) is a promising approach for treating obesity and metabolic disorders. However, obtaining sufficient amounts of functional BAT or brown adipocytes for transplantation remains a major challenge. In this study, ... ...

Abstract	Transplantation of brown adipose tissue (BAT) is a promising approach for treating obesity and metabolic disorders. However, obtaining sufficient amounts of functional BAT or brown adipocytes for transplantation remains a major challenge. In this study, we developed a hydrogel that combining adipose acellular matrix (AAM) and GelMA and HAMA that can be adjusted for stiffness by modulating the duration of light-crosslinking. We used human white adipose tissue-derived microvascular fragments to create beige adipose organoids (BAO) that were encapsulated in either a soft or stiff AAM hydrogel. We found that BAOs cultivated in AAM hydrogels with high stiffness demonstrated increased metabolic activity and upregulation of thermogenesis-related genes. When transplanted into obese and type 2 diabetes mice, the HFD + BAO group showed sustained improvements in metabolic rate, resulting in significant weight loss and decreased blood glucose levels. Furthermore, the mice showed a marked reduction in nonalcoholic liver steatosis, indicating improved liver function. In contrast, transplantation of 2D-cultured beige adipocytes failed to produce these beneficial effects. Our findings demonstrate the feasibility of fabricating beige adipose organoids in vitro and administering them by injection, which may represent a promising therapeutic approach for obesity and diabetes.
MeSH term(s)	Humans ; Animals ; Mice ; Diet, High-Fat/adverse effects ; Diabetes Mellitus, Type 2/metabolism ; Hydrogels/pharmacology ; Adipose Tissue, Brown/metabolism ; Obesity/metabolism ; Adipose Tissue, White/metabolism ; Thermogenesis
Chemical Substances	Hydrogels
Language	English
Publishing date	2024-01-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.31191
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Article ; Online: A crystalline-amorphous interface engineering in Fe-doped Ni

Cao, Shuai / Fan, Xiaoming / Wei, Li / Cai, Ting / Lin, Yuping / Yang, Zeheng

Dalton transactions (Cambridge, England : 2003)

2023 Volume 52, Issue 18, Page(s) 5999–6007

Abstract: OER (oxygen evolution reaction) is a critical reaction in several storage and conversion systems for renewable and clean electrochemical energies, including solar fuel devices, metal-air batteries, as well as regenerative fuel and water splitting cells. ... ...

Abstract	OER (oxygen evolution reaction) is a critical reaction in several storage and conversion systems for renewable and clean electrochemical energies, including solar fuel devices, metal-air batteries, as well as regenerative fuel and water splitting cells. Regarding the shortcomings of OER, apart from the sluggish kinetics and high reaction overpotential, the reaction rate and overpotential are difficult to be optimized simultaneously. Herein, a novel hierarchical particle-sheet-structured Fe-doped Ni
Language	English
Publishing date	2023-05-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d3dt00448a
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Article ; Online: Phytohormone Crosstalk of Cytokinin Biosynthesis and Signaling Family Genes in Moso Bamboo (

Bai, Yucong / Cai, Miaomiao / Dou, Yuping / Xie, Yali / Zheng, Huifang / Gao, Jian

International journal of molecular sciences

2023 Volume 24, Issue 13

Abstract: Cytokinin is widely involved in the regulation of plant growth, but its pathway-related genes have not been reported in Moso bamboo. In this study, a total of 129 candidate sequences were identified by bioinformatic methods. These included ... ...

Abstract	Cytokinin is widely involved in the regulation of plant growth, but its pathway-related genes have not been reported in Moso bamboo. In this study, a total of 129 candidate sequences were identified by bioinformatic methods. These included 15
MeSH term(s)	Plant Growth Regulators/metabolism ; Phylogeny ; Poaceae/genetics ; Indoleacetic Acids/metabolism ; Cytokinins/genetics ; Cytokinins/metabolism ; Gene Expression Regulation, Plant
Chemical Substances	Plant Growth Regulators ; Indoleacetic Acids ; Cytokinins
Language	English
Publishing date	2023-06-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241310863
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Article ; Online: Structural and functional properties of Maillard-reacted casein phosphopeptides with different carbohydrates.

Yuan, Meng / Cao, Yu / Zheng, Haoyang / Chen, Kunlin / Lu, Yuping / Wang, Jing / Zhu, Liqin / Chen, Ming / Cai, Zhipeng / Shen, Yonggen

Food science and biotechnology

2023 Volume 33, Issue 7, Page(s) 1603–1614

Language	English
Publishing date	2023-12-20
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2000008-X
ISSN	2092-6456 ; 1226-7708
ISSN (online)	2092-6456
ISSN	1226-7708
DOI	10.1007/s10068-023-01463-2
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Article ; Online: Combining High-Density Fat and Condensed Low-Density Fat Injections for Precise Facial Rejuvenation.

Zhu, Huidong / Shi, Hui / Li, Jian / Liao, Yunjun / Cai, Junrong / Lu, Feng / Quan, Yuping

Aesthetic plastic surgery

2024

Abstract: Background: Facial aging involves ptosis, adipose atrophy, and skeletal resorption. Depletion of adipose tissue primarily affects the deep facial fat compartment, leading to facial depression or ptosis, accompanied by atrophy of the superficial ... ...

Abstract	Background: Facial aging involves ptosis, adipose atrophy, and skeletal resorption. Depletion of adipose tissue primarily affects the deep facial fat compartment, leading to facial depression or ptosis, accompanied by atrophy of the superficial compartment. Restoring volume in the deep fat compartment is crucial for facial rejuvenation, while enhancing its supportive properties is also important. The superficial fat compartment contains small-sized adipocytes, and autologous fat grafting is a popular approach. However, variability in fat retention, homogeneity, and processing methods can impact outcomes, necessitating careful selection of a suitable fat processing material for precise facial fat grafting. Method: A retrospective study was conducted on 50 patients who underwent facial augmentation using combined transplantation of high-density fat (HDF) and condensed low-density fat (CLDF) and 25 patients who underwent conventional Coleman fat grafting. Coleman fat was harvested by standard technique and the adipose tissue was divided into HDF and CLDF fractions through centrifugation. Subsequently, the low-density fat fraction was subjected to a process involving physical disruption followed by additional centrifugation to obtain CLDF. The CLDF fraction was consequently injected into the pre-SMAS subcutaneous layer of the superficial fat compartments. Patient satisfaction was evaluated using a typical Likert scale. Photographs were taken and imageological examinations were performed before and after treatment. Result: The CLDF+HDF grafting group demonstrated a significantly shorter duration of swelling (6.0 ± 1.2 to 12.6 ± 3.3 days) and higher level of patient satisfaction when compared to the Coleman fat group. No serious complications were observed among all the patients who received the injections. Conclusion: The use of this new treatment approach allows for precise fat transplantation in facial regions. The use of high-concentration fat filling for deep facial layers and CLDF filling for superficial layers is a safe and effective treatment plan for facial rejuvenation. Level of evidence iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
Language	English
Publishing date	2024-03-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	532791-x
ISSN	1432-5241 ; 0364-216X
ISSN (online)	1432-5241
ISSN	0364-216X
DOI	10.1007/s00266-024-03953-y
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Article ; Online: Transplantation of in vitro prefabricated adipose organoids attenuates skin fibrosis by restoring subcutaneous fat and inducing dermal adipogenesis.

Quan, Yuping / Zhang, Yuteng / Li, Jian / Lu, Feng / Cai, Junrong

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2023 Volume 37, Issue 8, Page(s) e23076

Abstract: Localized scleroderma is a complex autoimmune disease characterized by dermal fibrosis and loss of cutaneous fat. While cytotherapy offers a promising treatment option, stem cell transplantation results in low survival rates and fails in target cell ... ...

Abstract	Localized scleroderma is a complex autoimmune disease characterized by dermal fibrosis and loss of cutaneous fat. While cytotherapy offers a promising treatment option, stem cell transplantation results in low survival rates and fails in target cell differentiation. In this study, we aimed to prefabricate syngeneic adipose organoids (ad-organoids) using microvascular fragments (MVFs) via three-dimensional (3D) culturing and transplant them beneath the fibrotic skin to restore subcutaneous fat and reverse the pathological manifestation of localized scleroderma. We employed 3D culturing of syngeneic MVFs with stepwise angiogenic and adipogenic induction to produce ad-organoids and evaluated their microstructure and paracrine function in vitro. C57/BL6 mice with induced skin scleroderma were treated with adipose-derived stem cells (ASCs), adipocytes, ad-organoids, and Matrigel, and the therapeutic effect was assessed histologically. Our results showed that ad-organoids derived from MVF contained mature adipocytes and a well-established vessel network, secreted multiple adipokines, promoted adipogenic differentiation of ASCs, and suppressed proliferation and migration of scleroderma fibroblasts. Subcutaneous transplantation of ad-organoids reconstructed the subcutaneous fat layer and stimulated dermal adipocyte regeneration in bleomycin-induced scleroderma skin. It reduced collagen deposition and dermal thickness, attenuating dermal fibrosis. Moreover, ad-organoids suppressed macrophage infiltration and promoted angiogenesis in the skin lesion. In conclusion, 3D culturing of MVFs with stepwise angiogenic and adipogenic induction is an effective strategy for the fabrication of ad-organoids, and the transplantation of prefabricated ad-organoids can improve skin sclerosis by restoring cutaneous fat and attenuating skin fibrosis. These findings offer a promising therapeutic approach for the treatment of localized scleroderma.
MeSH term(s)	Animals ; Mice ; Adipogenesis ; Scleroderma, Localized ; Adiposity ; Obesity ; Subcutaneous Fat
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.202202117R
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Article ; Online: A superior mulberry-like nanoparticle NiB binary catalyst for borohydride oxidation.

Cai, Jinliang / Yi, Caini / Xie, Yuxin / Yang, Ying / Yang, Hang / Liu, Yuping / Chen, Changguo / Yu, Danmei / Zhou, Xiaoyuan

Chemical communications (Cambridge, England)

2024 Volume 60, Issue 18, Page(s) 2540–2543

Abstract: A NiB binary catalyst with a unique mulberry-like nanoparticle morphology has been prepared by one-step electrodeposition. The NiB-0.2 catalyst exhibits excellent catalytic activity, selectivity, and stability for the borohydride oxidation reaction. ... ...

Abstract	A NiB binary catalyst with a unique mulberry-like nanoparticle morphology has been prepared by one-step electrodeposition. The NiB-0.2 catalyst exhibits excellent catalytic activity, selectivity, and stability for the borohydride oxidation reaction. Moreover, a direct borohydride fuel cell using the NiB-0.2 catalyst anode can deliver a peak power density of 453 mW cm
Language	English
Publishing date	2024-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/d3cc05899a
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