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  1. Article ; Online: New insights into the regulation of bile acids synthesis during the early stages of liver regeneration: A human and experimental study.

    Uriarte, Iker / Santamaria, Eva / López-Pascual, Amaya / Monte, María J / Argemí, Josepmaria / Latasa, M Ujue / Adán-Villaescusa, Elena / Irigaray, Ainara / Herranz, Jose M / Arechederra, María / Basualdo, Jorge / Lucena, Felipe / Corrales, Fernando J / Rotellar, Fernando / Pardo, Fernando / Merlen, Gregory / Rainteau, Dominique / Sangro, Bruno / Tordjmann, Thierry /
    Berasain, Carmen / Marín, Jose J G / Fernández-Barrena, Maite G / Herrero, Ignacio / Avila, Matias A

    Biochimica et biophysica acta. Molecular basis of disease

    2024  Volume 1870, Issue 5, Page(s) 167166

    Abstract: Background and aims: Liver regeneration is essential for the preservation of homeostasis and survival. Bile acids (BAs)-mediated signaling is necessary for liver regeneration, but BAs levels need to be carefully controlled to avoid hepatotoxicity. We ... ...

    Abstract Background and aims: Liver regeneration is essential for the preservation of homeostasis and survival. Bile acids (BAs)-mediated signaling is necessary for liver regeneration, but BAs levels need to be carefully controlled to avoid hepatotoxicity. We studied the early response of the BAs-fibroblast growth factor 19 (FGF19) axis in healthy individuals undergoing hepatectomy for living donor liver transplant. We also evaluated BAs synthesis in mice upon partial hepatectomy (PH) and acute inflammation, focusing on the regulation of cytochrome-7A1 (CYP7A1), a key enzyme in BAs synthesis from cholesterol.
    Methods: Serum was obtained from twelve human liver donors. Mice underwent 2/3-PH or sham-operation. Acute inflammation was induced with bacterial lipopolysaccharide (LPS) in mice fed control or antoxidant-supplemented diets. BAs and 7α-hydroxy-4-cholesten-3-one (C4) levels were measured by HPLC-MS/MS; serum FGF19 by ELISA. Gene expression and protein levels were analyzed by RT-qPCR and western-blot.
    Results: Serum BAs levels increased after PH. In patients with more pronounced hypercholanemia, FGF19 concentrations transiently rose, while C4 levels (a readout of CYP7A1 activity) dropped 2 h post-resection in all cases. Serum BAs and C4 followed the same pattern in mice 1 h after PH, but C4 levels also dropped in sham-operated and LPS-treated animals, without marked changes in CYP7A1 protein levels. LPS-induced serum C4 decline was attenuated in mice fed an antioxidant-supplemented diet.
    Conclusions: In human liver regeneration FGF19 upregulation may constitute a protective response from BAs excess during liver regeneration. Our findings suggest the existence of post-translational mechanisms regulating CYP7A1 activity, and therefore BAs synthesis, independent from CYP7A1/Cyp7a1 gene transcription.
    Language English
    Publishing date 2024-04-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 60-7
    ISSN 1879-260X ; 1879-2596 ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-260X ; 1879-2596 ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbadis.2024.167166
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: L'onde U de l'électrocardiogramme.

    MERLEN, J F

    France Medecine

    2003  Volume 20, Issue 4, Page(s) 5–8; passim

    Title translation The U wave of the electrocardiogram.
    MeSH term(s) Arrhythmias, Cardiac ; Brugada Syndrome ; Cardiac Conduction System Disease ; Electrocardiography ; Heart Conduction System ; Humans
    Language French
    Publishing date 2003-01-17
    Publishing country France
    Document type Journal Article
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Hepatic deletion of serine palmitoyl transferase 2 impairs ceramide/sphingomyelin balance, bile acids homeostasis and leads to liver damage in mice.

    Lallement, Justine / Raho, Ilyès / Merlen, Grégory / Rainteau, Dominique / Croyal, Mikael / Schiffano, Melody / Kassis, Nadim / Doignon, Isabelle / Soty, Maud / Lachkar, Floriane / Krempf, Michel / Van Hul, Matthias / Cani, Patrice D / Foufelle, Fabienne / Amouyal, Chloé / Le Stunff, Hervé / Magnan, Christophe / Tordjmann, Thierry / Cruciani-Guglielmacci, Céline

    Biochimica et biophysica acta. Molecular and cell biology of lipids

    2023  Volume 1868, Issue 8, Page(s) 159333

    Abstract: Ceramides (Cer) have been shown as lipotoxic inducers, which disturb numerous cell-signaling pathways, leading to metabolic disorders such as type 2 diabetes. In this study, we aimed to determine the role of de novo hepatic ceramide synthesis in energy ... ...

    Abstract Ceramides (Cer) have been shown as lipotoxic inducers, which disturb numerous cell-signaling pathways, leading to metabolic disorders such as type 2 diabetes. In this study, we aimed to determine the role of de novo hepatic ceramide synthesis in energy and liver homeostasis in mice. We generated mice lacking serine palmitoyltransferase 2 (Sptlc2), the rate limiting enzyme of ceramide de novo synthesis, in liver under albumin promoter. Liver function, glucose homeostasis, bile acid (BA) metabolism and hepatic sphingolipids content were assessed using metabolic tests and LC-MS. Despite lower expression of hepatic Sptlc2, we observed an increased concentration of hepatic Cer, associated with a 10-fold increase in neutral sphingomyelinase 2 (nSMase2) expression, and a decreased sphingomyelin content in the liver. Sptlc2
    MeSH term(s) Animals ; Mice ; Bile Acids and Salts/metabolism ; Ceramides/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Glucose/metabolism ; Homeostasis ; Liver/metabolism ; Serine/metabolism ; Serine C-Palmitoyltransferase/metabolism ; Sphingolipids/metabolism ; Sphingomyelins/metabolism
    Chemical Substances Bile Acids and Salts ; Ceramides ; Glucose (IY9XDZ35W2) ; Serine (452VLY9402) ; Serine C-Palmitoyltransferase (EC 2.3.1.50) ; Sphingolipids ; Sphingomyelins ; Sptlc2 protein, mouse (EC 2.3.1.50)
    Language English
    Publishing date 2023-05-22
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbalip.2023.159333
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The diagnosis of a haemophilia A carrier over 2 decades.

    Dubé, Evemie / Gauthier, Julie / Merlen, Clémence / Bonnefoy, Arnaud / Couture, Francoise / Lillicrap, David / Rivard, Georges-Etienne

    Haemophilia : the official journal of the World Federation of Hemophilia

    2020  Volume 27, Issue 1, Page(s) e133–e136

    MeSH term(s) Factor VIII/genetics ; Female ; Genetic Carrier Screening ; Hemophilia A/diagnosis ; Hemophilia A/genetics ; Heterozygote ; Humans ; Pregnancy ; Prenatal Diagnosis
    Chemical Substances Factor VIII (9001-27-8)
    Language English
    Publishing date 2020-06-11
    Publishing country England
    Document type Letter
    ZDB-ID 1229713-6
    ISSN 1365-2516 ; 1351-8216 ; 1355-0691
    ISSN (online) 1365-2516
    ISSN 1351-8216 ; 1355-0691
    DOI 10.1111/hae.14073
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Beyond MEN1, when to think about MEN4? Retrospective study on 5600 patients in the French population & literature review.

    Chevalier, Benjamin / Coppin, Lucie / Romanet, Pauline / Cuny, Thomas / Maiza, Jean-Christophe / Abeillon, Juliette / Forestier, Julien / Walter, Thomas / Gilly, Olivier / Le Bras, Maëlle / Smati, Sarra / Nunes, Marie Laure / Geslot, Aurore / Grunenwald, Solange / Mouly, Céline / Arnault, Gwenaelle / Wagner, Kathy / Koumakis, Eugénie / Cortet-Rudelli, Christine /
    Merlen, Émilie / Jannin, Arnaud / Espiard, Stéphanie / Morange, Isabelle / Baudin, Éric / Cavaille, Mathias / Tauveron, Igor / Teissier, Marie-Pierre / Borson-Chazot, Françoise / Mirebeau-Prunier, Delphine / Savagner, Frédérique / Pasmant, Éric / Giraud, Sophie / Vantyghem, Marie-Christine / Goudet, Pierre / Barlier, Anne / Cardot-Bauters, Catherine / Odou, Marie Françoise

    The Journal of clinical endocrinology and metabolism

    2024  

    Abstract: Context: Germline CDKN1B variants predispose patients to multiple endocrine neoplasia type 4 (MEN4), a rare MEN1-like syndrome, with <100 reported cases since its discovery in 2006. Although CDKN1B mutations are frequently suggested to explain cases of ... ...

    Abstract Context: Germline CDKN1B variants predispose patients to multiple endocrine neoplasia type 4 (MEN4), a rare MEN1-like syndrome, with <100 reported cases since its discovery in 2006. Although CDKN1B mutations are frequently suggested to explain cases of genetically-negative MEN1, the prevalence and phenotype of MEN4 patients is poorly known, and genetic counseling is unclear.
    Objective: To evaluate the prevalence of MEN4 in MEN1-suspected patients and characterize the phenotype of MEN4 patients.
    Design: Retrospective observational nationwide study. Narrative review of literature and variant class reassessment.
    Patients: We included all adult patients with class 3/4/5 CDKN1B variants identified by the laboratories from the French TENGEN network between 2015 and 2022 through germline genetic testing for MEN1 suspicion. After class reassessment, we compared the phenotype of symptomatic patients with class 4/5 CDKN1B variants, i.e. with genetically-confirmed MEN4 diagnosis, in our series and in literature with 66 matched MEN1 patients from the UMD-MEN1 database.
    Results: From 5600 MEN1-suspected patients analyzed, four patients with class 4/5 CDKN1B variant were found (0.07%). They presented with multiple duodenal NET, PHPT and adrenal nodule, isolated PHPT, PHPT and pNET. We listed 29 patients with CDKN1B class 4/5 variants from literature. Compared to matched MEN1 patients, MEN4 patients presented lower NET incidence and older age at PHPT diagnosis.
    Conclusion: The prevalence of MEN4 is low. PHPT and PA represent the main associated lesions, NETs are rare. Our results suggest a milder and later phenotype than in MEN1. Our observations will help to improve genetic counseling and management of MEN4 families.
    Language English
    Publishing date 2024-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3029-6
    ISSN 1945-7197 ; 0021-972X
    ISSN (online) 1945-7197
    ISSN 0021-972X
    DOI 10.1210/clinem/dgae055
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Optical near-field mapping of plasmonic nanostructures prepared by nanosphere lithography.

    Kolhatkar, Gitanjali / Merlen, Alexandre / Zhang, Jiawei / Dab, Chahinez / Wallace, Gregory Q / Lagugné-Labarthet, François / Ruediger, Andreas

    Beilstein journal of nanotechnology

    2018  Volume 9, Page(s) 1536–1543

    Abstract: We introduce a simple, fast, efficient and non-destructive method to study the optical near-field properties of plasmonic nanotriangles prepared by nanosphere lithography. Using a rectangular Fourier filter on the blurred signal together with filtering ... ...

    Abstract We introduce a simple, fast, efficient and non-destructive method to study the optical near-field properties of plasmonic nanotriangles prepared by nanosphere lithography. Using a rectangular Fourier filter on the blurred signal together with filtering of the lower spatial frequencies to remove the far-field contribution, the pure near-field contributions of the optical images were extracted. We performed measurements using two excitation wavelengths (532.1 nm and 632.8 nm) and two different polarizations. After the processing of the optical images, the distribution of hot spots can be correlated with the topography of the structures, as indicated by the presence of brighter spots at the apexes of the nanostructures. This technique is validated by comparison of the results to numerical simulations, where agreement is obtained, thereby confirming the near-field nature of the images. Our approach does not require any advanced equipment and we suggest that it could be applied to any type of sample, while keeping the measurement times reasonably short.
    Language English
    Publishing date 2018-05-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2583584-1
    ISSN 2190-4286
    ISSN 2190-4286
    DOI 10.3762/bjnano.9.144
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  7. Article ; Online: Study of potential transfer of aluminum to the brain via the olfactory pathway.

    Chalansonnet, Monique / Carabin, Nathalie / Boucard, Stéphane / Merlen, Lise / Melczer, Mathieu / Antoine, Guillaume / Devoy, Jérôme / Remy, Aurélie / Gagnaire, François

    Toxicology letters

    2018  Volume 283, Page(s) 77–85

    Abstract: Many employees in the aluminum industry are exposed to a range of aluminum compounds by inhalation, and the presence of ultrafine particles in the workplace has become a concern to occupational health professionals. Some metal salts and metal oxides have ...

    Abstract Many employees in the aluminum industry are exposed to a range of aluminum compounds by inhalation, and the presence of ultrafine particles in the workplace has become a concern to occupational health professionals. Some metal salts and metal oxides have been shown to enter the brain through the olfactory route, bypassing the blood-brain barrier, but few studies have examined whether aluminum compounds also use this pathway. In this context, we sought to determine whether aluminum was found in rat olfactory bulbs and whether its transfer depended on physicochemical characteristics such as solubility and granulometry. Aluminum salts (chloride and fluoride) and various nanometric aluminum oxides (13nm, 20nm and 40-50nm) were administered to rats by intranasal instillation through one nostril (10μg Al/30μL for 10days). Olfactory bulbs (ipsilateral and contralateral relative to instilled nostril) were harvested and the aluminum content was determined by graphite furnace atomic absorption spectrometry after tissue mineralization. Some transfer of aluminum salts to the central nervous system via the olfactory route was observed, with the more soluble aluminum chloride being transferred at higher levels than aluminum fluoride. No cerebral translocation of any of the aluminas studied was detected.
    MeSH term(s) Administration, Intranasal ; Algorithms ; Aluminum Compounds/metabolism ; Aluminum Compounds/pharmacokinetics ; Aluminum Compounds/toxicity ; Animals ; Brain/metabolism ; Chlorides/metabolism ; Chlorides/pharmacokinetics ; Chlorides/toxicity ; Fluorides/metabolism ; Fluorides/pharmacokinetics ; Fluorides/toxicity ; Male ; Nasal Mucosa/pathology ; Olfactory Bulb/metabolism ; Olfactory Pathways/metabolism ; Rats ; Rats, Sprague-Dawley ; Spectrophotometry, Atomic
    Chemical Substances Aluminum Compounds ; Chlorides ; aluminum chloride (3CYT62D3GA) ; Fluorides (Q80VPU408O) ; aluminum fluoride (Z77H3IKW94)
    Language English
    Publishing date 2018-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 433788-8
    ISSN 1879-3169 ; 0378-4274
    ISSN (online) 1879-3169
    ISSN 0378-4274
    DOI 10.1016/j.toxlet.2017.11.027
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  8. Article: Effects of co-exposure to CS

    Chalansonnet, Monique / Carreres-Pons, Maria / Venet, Thomas / Thomas, Aurélie / Merlen, Lise / Boucard, Stéphane / Cosnier, Frédéric / Nunge, Hervé / Bonfanti, Elodie / Llorens, Jordi / Campo, Pierre / Pouyatos, Benoît

    Journal of occupational medicine and toxicology (London, England)

    2020  Volume 15, Page(s) 9

    Abstract: Background: Carbon disulfide (CS: Methods: Rats were exposed for 4 weeks (5 days/week, 6 h/day) to a band noise at 106 dB SPL either alone or combined with continuous (63 ppm or 250 ppm) or intermittent (15 min/h or 2 × 15 min/h at 250 ppm) CS: ... ...

    Abstract Background: Carbon disulfide (CS
    Methods: Rats were exposed for 4 weeks (5 days/week, 6 h/day) to a band noise at 106 dB SPL either alone or combined with continuous (63 ppm or 250 ppm) or intermittent (15 min/h or 2 × 15 min/h at 250 ppm) CS
    Results: The results obtained here confirmed that CS
    Conclusion: These results reveal that short periods of CS
    Language English
    Publishing date 2020-05-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2223190-0
    ISSN 1745-6673
    ISSN 1745-6673
    DOI 10.1186/s12995-020-00260-5
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  9. Article ; Online: A prospective surveillance study of inhibitor development in haemophilia A patients following a population switch to a third-generation B-domain-deleted recombinant factor VIII.

    Dubé, E / Bonnefoy, A / Merlen, C / Castilloux, J-F / Cloutier, S / Demers, C / Sabapathy, C A / St-Louis, J / Vezina, C / Warner, M / Rivard, G-É

    Haemophilia : the official journal of the World Federation of Hemophilia

    2018  Volume 24, Issue 2, Page(s) 236–244

    Abstract: Introduction: Following a provincial tender, most subjects with haemophilia A in Quebec switched their treatment to a third-generation recombinant B-domain-deleted factor VIII (FVIII).: Aim: Our objective was to evaluate the incidence of inhibitor ... ...

    Abstract Introduction: Following a provincial tender, most subjects with haemophilia A in Quebec switched their treatment to a third-generation recombinant B-domain-deleted factor VIII (FVIII).
    Aim: Our objective was to evaluate the incidence of inhibitor development and FVIII recovery in patients following the switch of factor replacement therapy.
    Methods: One hundred and thirty-five subjects were enrolled and tested for FVIII activity and inhibitors every 6 months during 1 year. Subjects with mild haemophilia A or current inhibitors were excluded. Data on demographics, bleeds and FVIII usage were collected.
    Results: A total of 125 switchers and 10 non-switchers were enrolled. Most subjects had severe haemophilia A (95.6%) and were on prophylaxis (89.6%). Mean FVIII recovery was similar at 0, 6 and 12 months postswitch. Two switchers developed de novo inhibitors in the 6 months postswitch, one of which was transient. No recurrent inhibitor was observed. A small but significant increase in FVIII usage was observed for adult switchers and the whole cohort of switchers and non-switchers. There was an increase in the annualized bleeding rate (ABR) for non-joint bleeds for the whole cohort of switchers. However, no significant differences were observed in ABR for joint bleeds.
    Conclusion: Our surveillance study shows comparable inhibitor development to similar published studies. A significant increase in FVIII utilization was noted for the whole cohort, switchers and non-switchers. Lastly, no clinically significant changes were observed in ABR for joint bleeds, but a difference for non-joint bleed ABRs was observed in switchers.
    MeSH term(s) Adult ; Factor VIII/therapeutic use ; Female ; Hemophilia A/drug therapy ; Humans ; Male ; Prospective Studies
    Chemical Substances Factor VIII (9001-27-8)
    Language English
    Publishing date 2018-02-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1229713-6
    ISSN 1365-2516 ; 1351-8216 ; 1355-0691
    ISSN (online) 1365-2516
    ISSN 1351-8216 ; 1355-0691
    DOI 10.1111/hae.13410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Purpuras by capillary damage.

    DOUMER, E / MERLEN, J F / DUBRULLE, P

    L'echo medical du nord

    2007  Volume 19, Issue 10, Page(s) 244

    Title translation Purpuras par atteinte capillaire.
    MeSH term(s) Animals ; Gastropoda ; Humans ; Purpura
    Language French
    Publishing date 2007-12-15
    Publishing country France
    Document type Journal Article
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