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  1. Article ; Online: Erratum: Alves, J., et al. Correlations between Basal Trace Minerals and Hormones in Middle and Long-Distance High-Level Male Runners.

    Alves, Javier / Barrientos, Gema / Toro, Víctor / Grijota, Francisco Javier / Muñoz, Diego / Maynar, Marcos

    International journal of environmental research and public health

    2021  Volume 18, Issue 5

    Abstract: The authors wish to correct the following erratum in this paper [ ... ]. ...

    Abstract The authors wish to correct the following erratum in this paper [...].
    Language English
    Publishing date 2021-02-25
    Publishing country Switzerland
    Document type Published Erratum
    ISSN 1660-4601
    ISSN (online) 1660-4601
    DOI 10.3390/ijerph18052289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Chemical Fingerprinting, Aorta Endothelium Relaxation Effect, and Enzymatic Inhibition of Canelo (Drimys winteri J. R. Forst. & G. Forst, (D.C) A. Gray, Family Winteraceae) Fruits

    Barrientos, Ruth E. / Romero-Parra, Javier / Cifuentes, Fredi / Palacios, Xabier / Romero-Jola, Néstor Jaime / Paredes, Adrián / Vargas-Arana, Gabriel / Simirgiotis, Mario J.

    Foods. 2023 July 01, v. 12, no. 13

    2023  

    Abstract: Drimys winteri J.R. Forst. & G. Forst (D.C) G. Gray, var. chilensis (canelo) is an endemic tree ...

    Abstract Drimys winteri J.R. Forst. & G. Forst (D.C) G. Gray, var. chilensis (canelo) is an endemic tree from Chile. Since pre-Columbian times, it has produced a fruit known as the canelo pepper, (pimienta de canelo) or Foye pepper, which can be used as a spice. The chemical and biological analysis of canelo fruits is reported for the first time in this study, that is, its phenolic fingerprinting by UHPLC-PDA- Q-orbitrap MS, the antioxidant activity, the enzymatic inhibitory activity, and its relaxation effects on rat aorta. The proximal composition and the mineral content (Ca: 1.45 ± 0.03 mg/100 g; Mg: 7.72 ± 0.03 mg/100 g; Fe: 4.54 ± 0.21 mg/100 g; Zn: 2.99 ± 0.02 mg/100 g; Mn: 1.08 ± 0.03 mg/100 g; Cu: 0.82 ± 0.02 mg/100 g; K: 53.03 ± 0.20 mg/100 g; Na: 0.087 ± 0.00 mg/100 g) are also reported. The canelo fruits showed a total phenolic content of 57.33 ± 0.82 mg GAE/g dry weight. In addition, the total flavonoid content was 38.42 ± 1.32 mg equivalent of QE/g dry weight. The antioxidant activity was evaluated by employing DPPH and ABTS methods (IC₅₀ of 6.65 ± 0.5 and 9.5 ± 0.05 μg/mL, respectively), ORAC (25.33 ± 1.2 μmol Trolox/g dry plant) and FRAP (45.56 ± 1.32 μmol Trolox/g dry plant). The enzymatic inhibition of acetylcholinesterase, butyrylcholinesterase, and tyrosinase (IC₅₀: 1.94 ± 0.07, 2.73 ± 0.05, and 9.92 ± 0.05 µg extract/mL, respectively) is also reported. Canelo extract led to an 89% relaxation of rat aorta. Our results confirm that D. winteri fruits are a rich source of secondary metabolites and can inhibit enzymes associated with neurodegenerative diseases; the results also suggest that canelo may induce a potentially hypotensive effect in rat aorta. The study demonstrates the medicinal properties of canelo fruit and spice.
    Keywords Drimys winteri ; acetylcholinesterase ; antihypertensive effect ; antioxidant activity ; aorta ; cholinesterase ; endothelium ; enzyme inhibition ; flavonoids ; fruits ; indigenous species ; mineral content ; pepper ; rats ; secondary metabolites ; Chile
    Language English
    Dates of publication 2023-0701
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12132580
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Correction: Distance-dependent cellular palmitoylation of de-novo-designed sequences and their translocation to plasma membrane subdomains (J. Cell Sci. 115, 3119-3130).

    Navarro-Lérida, Inmaculada / Álvarez-Barrientos, Alberto / Gavilanes, Francisco / Rodríguez-Crespo, Ignacio

    Journal of cell science

    2018  Volume 131, Issue 10

    Language English
    Publishing date 2018-06-04
    Publishing country England
    Document type Journal Article ; Published Erratum
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.219634
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Expression of Concern: N-terminal palmitoylation within the appropriate amino acid environment conveys on NOS2 the ability to progress along the intracellular sorting pathways. J. Cell Sci. doi: 10.1242/jcs.02878.

    Navarro-Lérida, Inmaculada / Álvarez-Barrientos, Alberto / Rodríguez-Crespo, Ignacio

    Journal of cell science

    2017  Volume 130, Issue 19, Page(s) 3416

    Language English
    Publishing date 2017-08-30
    Publishing country England
    Document type Journal Article ; Expression of Concern
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.209999
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  5. Article ; Online: Expression of Concern: Distance-dependent cellular palmitoylation of de-novo-designed sequences and their translocation to plasma membrane subdomains. J. Cell Sci. (2002). 115, 3119-3130.

    Navarro-Lérida, Inmaculada / Álvarez-Barrientos, Alberto / Gavilanes, Francisco / Rodríguez-Crespo, Ignacio

    Journal of cell science

    2017  Volume 130, Issue 19, Page(s) 3414

    Language English
    Publishing date 2017-08-30
    Publishing country England
    Document type Journal Article ; Expression of Concern
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.209973
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Expression of Concern: Induction of nitric oxide synthase-2 proceeds with the concomitant downregulation of the endogenous caveolin levels. J. Cell Sci. doi: 10.1242/jcs.01002.

    Navarro-Lérida, Inmaculada / Portolés, María Teresa / Barrientos, Alberto Álvarez / Gavilanes, Francisco / Boscá, Lisardo / Rodríguez-Crespo, Ignacio

    Journal of cell science

    2017  Volume 130, Issue 19, Page(s) 3415

    Language English
    Publishing date 2017-08-30
    Publishing country England
    Document type Journal Article ; Expression of Concern
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.209981
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  7. Article ; Online: INVITED REVIEW: Ketone biology: the shifting paradigm of ketones and ketosis in the dairy cow.

    Rico, J Eduardo / Barrientos-Blanco, Mario A

    Journal of dairy science

    2024  

    Abstract: Ketosis is currently regarded as a major metabolic disorder of dairy cows, reflective of the animal's efforts to adapt to energy deficit while transitioning into lactation. Currently viewed as a pathology by some, ketosis is associatively implicated in ... ...

    Abstract Ketosis is currently regarded as a major metabolic disorder of dairy cows, reflective of the animal's efforts to adapt to energy deficit while transitioning into lactation. Currently viewed as a pathology by some, ketosis is associatively implicated in milk production losses and peripartal health complications that increase the risk of early removal of cows from the herd, thus carrying economic losses for dairy farmers, and jeopardizing the sustainability of the dairy industry. Despite decades of intense research in the mitigation of ketosis and its sequelae, our ability to lessen its purported impacts remains limited. Moreover, the association of ketosis to reduced milk production and peripartal disease is often erratic and likely mired by concurrent potential confounders. In this review we discuss the potential reasons for these apparent paradoxes in the light of currently available evidence, with a focus on the limitations of observational research, and the necessary steps to unambiguously identify the impacts of ketosis on cow health and performance via controlled randomized experimentation. A nuanced perspective is proposed that considers the dissociation of ketosis -as a disease- from healthy hyperketonemia. Furthermore, in consideration of a growing body of evidence that highlights positives roles of ketones in the mitigation of metabolic dysfunction and chronic diseases, we consider the hypothetical functions of ketones as health-promoting metabolites and ponder on their potential usefulness to enhance dairy cow health and productivity.
    Language English
    Publishing date 2024-01-19
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2023-23904
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  8. Article ; Online: Rapid Cryopurification of the Yeast Mitochondrial Ribosome.

    Pang, Hong Weng / Barrientos, Antoni

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2661, Page(s) 133–141

    Abstract: Cryogenic milling, or cryomilling, involves the use of liquid nitrogen to lower the temperature of the biological material and/or the milling process. When applied to the study of subcellular or suborganellar structures and processes, it allows for their ...

    Abstract Cryogenic milling, or cryomilling, involves the use of liquid nitrogen to lower the temperature of the biological material and/or the milling process. When applied to the study of subcellular or suborganellar structures and processes, it allows for their rapid extraction from whole cells frozen in the physiological state of choice. This approach has proven to be useful for the study of yeast mitochondrial ribosomes. Following cryomilling of 100 mL of yeast culture, conveniently tagged mitochondrial ribosomes can be immunoprecipitated and purified in native conditions. These ribosomes are suitable for the application of downstream approaches. These include mitoribosome profiling to analyze the mitochondrial translatome or mass spectrometry analyses to assess the mitoribosome proteome in normal growth conditions or under stress, as described in this method.
    MeSH term(s) Mitochondrial Ribosomes/metabolism ; Saccharomyces cerevisiae/genetics ; Ribosomes/metabolism ; Mitochondria/ultrastructure ; Mass Spectrometry ; Mitochondrial Proteins/metabolism
    Chemical Substances Mitochondrial Proteins
    Language English
    Publishing date 2023-05-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3171-3_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Systematic Analysis of Assembly Intermediates in Yeast to Decipher the Mitoribosome Assembly Pathway.

    Del'Olio, Samuel / Barrientos, Antoni

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2661, Page(s) 163–191

    Abstract: Studies of yeast mitoribosome assembly have been historically hampered by the difficulty of generating mitoribosome protein-coding gene deletion strains with a stable mitochondrial genome. The identification of mitochondrial DNA-stabilizing approaches ... ...

    Abstract Studies of yeast mitoribosome assembly have been historically hampered by the difficulty of generating mitoribosome protein-coding gene deletion strains with a stable mitochondrial genome. The identification of mitochondrial DNA-stabilizing approaches allows for the generation of a complete set of yeast deletion strains covering all mitoribosome proteins and known assembly factors. These strains can be used to analyze the integrity and assembly state of mitoribosomes by determining the sedimentation profile of these structures by sucrose gradient centrifugation of mitochondrial extracts, coupled to mass spectrometry analysis of mitoribosome composition. Subsequent hierarchical cluster analysis of mitoribosome subassemblies accumulated in mutant strains reveals details regarding the order of protein association during the mitoribosome biogenetic process. These strains also allow the expression of truncated protein variants to probe the role of mitochondrion-specific protein extensions, the relevance of protein cofactors, or the importance of RNA-protein interactions in functional sites of the mitoribosome. In this chapter, we will detail the methodology involved in these studies.
    MeSH term(s) Mitochondrial Ribosomes/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Mitochondria/genetics ; Mitochondria/metabolism ; Ribosomal Proteins/metabolism ; DNA, Mitochondrial/metabolism ; Mitochondrial Proteins/metabolism
    Chemical Substances Ribosomal Proteins ; DNA, Mitochondrial ; Mitochondrial Proteins
    Language English
    Publishing date 2023-05-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3171-3_11
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  10. Article ; Online: Gold Glyconanoparticles as Water-Soluble Polyvalent Models To Study Carbohydrate Interactions This work was supported by the DGICYT (PB96-0820), J.M.F. thanks the MEC for a predoctoral fellowship. A.G.B. thanks CSIC for financial support. We thank Prof. Martín-Lomas for his scientific and financial support.

    de La Fuente, Jesús M. / Barrientos, Africa G. / Rojas, Teresa C. / Rojo, Javier / Cañada, Javier / Fernández, Asunción / Penadés, Soledad

    Angewandte Chemie (International ed. in English)

    2001  Volume 40, Issue 12, Page(s) 2257–2261

    Language English
    Publishing date 2001-06-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    Database MEDical Literature Analysis and Retrieval System OnLINE

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