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Article ; Online: Strategies to recover blood donors during the COVID-19 pandemic: a tertiary level hospital experience.

Peña-Carillo, Hebert I / Rosa-Zamboni, Daniela de la / López-Victoria, Alicia B / López-Martínez, Briceida / Guerrero-Díaz, Ana C

Boletin medico del Hospital Infantil de Mexico

2022 Volume 79, Issue 5, Page(s) 300–309

Abstract: Background: The SARS-CoV-2 pandemic has challenged blood banks. In Mexico, donors decreased 22% between April and May 2020 compared to the same months in 2019. This study analyzed the effect of the strategies to recover donors (altruistic and family) in ...

Title translation	Estrategias para recuperar donantes de sangre durante la pandemia por COVID-19: experiencia de un hospital de tercer nivel.
Abstract	Background: The SARS-CoV-2 pandemic has challenged blood banks. In Mexico, donors decreased 22% between April and May 2020 compared to the same months in 2019. This study analyzed the effect of the strategies to recover donors (altruistic and family) in a tertiary pediatric care center during the pandemic. Methods: The Blood Bank of the Hospital Infantil de México Federico Gómez implemented strategies to obtain blood components to ensure self-sufficiency. The effect of these strategies on donor recovery was analyzed. Results: There were 7,315 eligible donors in 2019 and 5,070 in 2020. Blood component requirements decreased from 10,037 units in 2019 to 8,619 in 2020. The strategies aimed at attracting altruistic donors managed to increase the percentage of this type of donor when comparing the months in which these strategies were applied with the same months in 2019. In addition, it was observed that the greater the number of methods used simultaneously, the higher the percentage of altruistic donors (rho = 0.846, p = 0.002). In contrast, strategies aimed at attracting family donors did not increase the number of this type of donor. Conclusions: Actions to recruit altruistic donors increased the number of this type of donor to meet the hospital's needs.
MeSH term(s)	Humans ; Child ; COVID-19/epidemiology ; SARS-CoV-2 ; Pandemics ; Blood Donors ; Tertiary Care Centers
Language	English
Publishing date	2022-10-20
Publishing country	Mexico
Document type	Journal Article
ZDB-ID	730519-9
ISSN	1665-1146 ; 1665-1146 ; 0539-6115 ; 0539-6123
ISSN (online)	1665-1146
ISSN	1665-1146 ; 0539-6115 ; 0539-6123
DOI	10.24875/BMHIM.21000237
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Article ; Online: Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients.

Parra-Ortega, Israel / Vilchis-Ordoñez, Armando / López-Martínez, Briceida / Angeles-Floriano, Tania

Boletin medico del Hospital Infantil de Mexico

2021 Volume 78, Issue 3, Page(s) 171–180

Abstract: Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of ... ...

Abstract	Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term "qualified laboratories" involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.
MeSH term(s)	COVID-19/diagnosis ; COVID-19 Nucleic Acid Testing/methods ; COVID-19 Testing/methods ; Child ; Humans ; RNA, Viral/isolation & purification ; SARS-CoV-2/genetics
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-05-03
Publishing country	Mexico
Document type	Journal Article
ZDB-ID	730519-9
ISSN	1665-1146 ; 1665-1146 ; 0539-6115 ; 0539-6123
ISSN (online)	1665-1146
ISSN	1665-1146 ; 0539-6115 ; 0539-6123
DOI	10.24875/BMHIM.20000210
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Article ; Online: Enrichment of effector memory T cells in the CD4 and CD8 T cell compartment during chronic graft versus host disease in children.

Parra-Ortega, Israel / Nájera-Martínez, Noemí / Gaytán-Morales, Félix / Castorena-Villa, Iván / López-Martínez, Briceida / Ortiz-Navarrete, Vianney / Olvera-Gómez, Irlanda

Transplant immunology

2023 Volume 81, Page(s) 101951

Abstract: Background: During allogeneic Hematopoietic stem cell transplantation (HSCT), frequent pathological scenarios include graft versus host disease (GVHD) and viral infections. We hypothesized if exogenous stimulus as alloantigen and viral antigens might ... ...

Abstract	Background: During allogeneic Hematopoietic stem cell transplantation (HSCT), frequent pathological scenarios include graft versus host disease (GVHD) and viral infections. We hypothesized if exogenous stimulus as alloantigen and viral antigens might impact on central and effector memory T cells in pediatric recipients. Patients and methods: Subjects included 21 pediatric recipients and 20 healthy children (control group). Peripheral blood samples of patients were collected along the first 712 days post-HSCT. T cell phenotyping of naïve, central, and effector memory T cells (TCMs and TEMs, respectively) was conducted using flow cytometry. Viral nucleic acids were detected using real-time PCR. Results: T cell reconstitution was not reached after 1 year post-HSCT. Chronic GVHD was associated with increased numbers of naïve CD4 T cells (p < 0.05) as well as an increase in TEM and TCM cells of the CD4 (p < 0.0001 and p < 0.05, respectively) and CD8 T cell TEM (p < 0.0001). and TCM (p < 0.001) populations too. Moreover, BK and Epstein-Barr viruses were the main viral pathogens detected (<10 Conclusion: During chronic GVHD, alloantigen persistence generates TEM cell enrichment among CD4 and CD8 T cells, and viral infections are associated with deficient recovery of T cells after HSCT.
MeSH term(s)	Humans ; Child ; Bronchiolitis Obliterans Syndrome ; Memory T Cells ; CD8-Positive T-Lymphocytes ; Graft vs Host Disease ; Virus Diseases ; Isoantigens ; Hematopoietic Stem Cell Transplantation
Chemical Substances	Isoantigens
Language	English
Publishing date	2023-11-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1160846-8
ISSN	1878-5492 ; 0966-3274
ISSN (online)	1878-5492
ISSN	0966-3274
DOI	10.1016/j.trim.2023.101951
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Article ; Online: Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients

Israel Parra-Ortega / Armando Vilchis-Ordoñez / Briceida López-Martínez / Tania Ángeles-Floriano

Boletín Médico del Hospital Infantil de México, Vol 78, Iss

2021 Volume 3

Abstract	Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term “qualified laboratories” involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.
Keywords	COVID-19. SARS-CoV-2. RT-PCR ; Pediatrics ; RJ1-570 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Permanyer
Document type	Article ; Online
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Article: Efficacy and safety of vitamin D supplementation in hospitalized COVID-19 pediatric patients: A randomized controlled trial.

Zurita-Cruz, Jessie / Fonseca-Tenorio, Jeffry / Villasís-Keever, Miguel / López-Alarcón, Mardia / Parra-Ortega, Israel / López-Martínez, Briceida / Miranda-Novales, Guadalupe

Frontiers in pediatrics

2022 Volume 10, Page(s) 943529

Abstract: Background: Some studies suggested that adequate levels of vitamin D (VD) decrease the risk of severe COVID-19. Information about the effectiveness of VD supplementation in children is scarce.: Objective: To assess the efficacy and safety of VD ... ...

Abstract	Background: Some studies suggested that adequate levels of vitamin D (VD) decrease the risk of severe COVID-19. Information about the effectiveness of VD supplementation in children is scarce. Objective: To assess the efficacy and safety of VD supplementation compared to the standard of care in hospitalized children with COVID-19. Patients and methods: An open-label randomized controlled single-blind clinical trial was carried out. We included patients from 1 month to 17 years, with moderate COVID-19, who required hospitalization and supplemental oxygen. They were randomized into two groups: the VD group, which received doses of 1,000 (children < 1 year) or 2,000 IU/day (from 1 to 17 years) and the group without VD (control). The outcome variables were the progression of oxygen requirement, the development of complications, and death. Statistical analysis: For comparison between groups, we used the chi-squared test or Fisher's exact test and the Mann-Whitney Results: From 24 March 2020 to 31 March 2021, 87 patients were eligible to participate in the trial; 45 patients were randomized: 20 to the VD group and 25 to the control group. There was no difference in general characteristics at baseline, including serum VD levels (median 13.8 ng/ml in the VD group and 11.4 ng/ml in the control group). Outcomes: 2/20 (10%) in the VD group vs. 9/25 (36%) in the control group progressed to a superior ventilation modality ( Conclusion: In this trial, VD supplementation in pediatric patients seems to decrease the risk of COVID-19 progression and death. More studies are needed to confirm these findings. Clinical trial registration: This protocol was registered on ClinicalTrials.gov with the registration number NCT04502667.
Language	English
Publishing date	2022-07-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711999-3
ISSN	2296-2360
ISSN	2296-2360
DOI	10.3389/fped.2022.943529
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Article ; Online: miRNAs in multiple sclerosis: A clinical approach.

Minutti-Zanella, Claudia / Bojalil-Álvarez, Lorena / García-Villaseñor, Elizabeth / López-Martínez, Briceida / Pérez-Turrent, Manuel / Murrieta-Álvarez, Iván / Ruiz-Delgado, Guillermo J / Argüelles, Guillermo J Ruiz

Multiple sclerosis and related disorders

2022 Volume 63, Page(s) 103835

Abstract: Micro-RNAs (miRNAs) are noncoding, single stranded segments of RNA measuring 19 to 25 nucleotides in length. They play an active role in autoimmune diseases, including multiple sclerosis (MS). These structures have been studied given their implication in ...

Abstract	Micro-RNAs (miRNAs) are noncoding, single stranded segments of RNA measuring 19 to 25 nucleotides in length. They play an active role in autoimmune diseases, including multiple sclerosis (MS). These structures have been studied given their implication in the process of diagnosis, disease development, treatment and prognosis of MS. Given the progressive and neurodegenerative nature of MS, miRNAs have been identified as critical mediators and molecular pinpoints of the disease, which poses them as excellent candidates for the obtention of suitable biomarkers and treatment targets. This review condenses recent findings on the role of miRNAs in multiple sclerosis, including their role in MS etiology and molecular mechanisms of the disease, exploitation of miRNAs as diagnostic tools and biomarkers, miRNAs as treatment option or target for MS, and their significance as predictors of disease prognosis.
MeSH term(s)	Autoimmune Diseases ; Biomarkers ; Humans ; MicroRNAs/genetics ; Multiple Sclerosis/diagnosis ; Multiple Sclerosis/genetics ; Multiple Sclerosis/therapy
Chemical Substances	Biomarkers ; MicroRNAs
Language	English
Publishing date	2022-04-30
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	2645330-7
ISSN	2211-0356 ; 2211-0348
ISSN (online)	2211-0356
ISSN	2211-0348
DOI	10.1016/j.msard.2022.103835
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Article ; Online: Presence of antibodies against HLA class I and class II molecules in children before and after allo-HSCT. Alloantibodies before and after HSCT.

Morán-Espinosa, Maricarmen / Angeles-Floriano, Tania / Parra-Ortega, Israel / Gaytán-Morales, Félix / Castorena-Villa, Iván / López-Martínez, Briceida / Ortiz-Navarrete, Vianney / Olvera-Gómez, Irlanda

HLA

2022 Volume 101, Issue 1, Page(s) 16–23

Abstract: A severe complication of allogeneic hematopoietic stem cell transplantation (HSCT) is graft failure (GF). Among others, donor-specific anti-HLA antibodies (DSA) are associated with graft rejection after allogeneic or haploidentical transplantation in ... ...

Abstract	A severe complication of allogeneic hematopoietic stem cell transplantation (HSCT) is graft failure (GF). Among others, donor-specific anti-HLA antibodies (DSA) are associated with graft rejection after allogeneic or haploidentical transplantation in adults. Knowledge of DSA and pediatric recipients is limited. Hence, we aimed to generate more information about the presence of DSA (pre- and post-HSCT) and the clinical outcomes (graft rejection and poor function) in children. We identified DSA in 27% of the patients. We observed a higher frequency (50%) of DSA-bearing patients with a benign disease diagnosis than those diagnosed with leukemia (16.66%). We observed graft rejection in one patient (with DSA against two alleles of HLA class I molecules) and poor function in three recipients during the first 30 days after HSCT in the absence of DSA. The presence of donor and nondonor HLA-specific antibodies decreased substantially after transplantation. After the transplant, we identified two patients with DSA specific for HLA class I molecules (independent of clinical relevance), and four recipients showed PGF in the absence of DSA. We were unable to establish any association between the presence of DSA and a clinical outcome: graft failure or prevalence of viral infection.
MeSH term(s)	Child ; Humans ; Alleles ; Hematopoietic Stem Cell Transplantation ; Isoantibodies ; Histocompatibility Antigens Class I ; Histocompatibility Antigens Class II
Chemical Substances	Isoantibodies ; Histocompatibility Antigens Class I ; Histocompatibility Antigens Class II
Language	English
Publishing date	2022-10-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2845111-9
ISSN	2059-2310 ; 2059-2302
ISSN (online)	2059-2310
ISSN	2059-2302
DOI	10.1111/tan.14817
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Article ; Online: Clinical risk profile associated with SARS-CoV-2 infection and complications in the emergency area of a pediatric COVID-19 center.

Olivar-López, Víctor / Leyva-Barrera, Ana / López-Martínez, Briceida / Parra-Ortega, Israel / Márquez-González, Horacio

Boletin medico del Hospital Infantil de Mexico

2020 Volume 77, Issue 5, Page(s) 221–227

Abstract: Background: In February 2020, the disease caused by the novel coronavirus (SARS-CoV-2), was classified as a pandemic. In the pediatric population, coronavirus disease (COVID)-19 has a reported mortality of less than 6% in complicated cases; however, the ...

Title translation	Perfil clínico de riesgo asociado a infección y complicaciones por SARS-CoV-2 en el área de urgencias de un centro pediátrico COVID-19.
Abstract	Background: In February 2020, the disease caused by the novel coronavirus (SARS-CoV-2), was classified as a pandemic. In the pediatric population, coronavirus disease (COVID)-19 has a reported mortality of less than 6% in complicated cases; however, the clinical characteristics and severity are not the same as those presented in the adult population. This study aimed to describe the clinical manifestations of patients younger than 18 years old and their association with the confirmation of the test and outcomes. Methods: We conducted an analytical cross-sectional study of symptoms suggestive for SARS-CoV-2 infection. All subjects with a confirmatory test for SARS-CoV-2 were included. Initial symptoms, history of influenza vaccination, and previous contact were documented, and mortality and the requirement for assisted mechanical ventilation were identified. The proportions of the variables were compared with the χ Results: Of a total of 510 subjects, 76 (15%) were positive for SARS-CoV-2. The associated symptoms were chest pain, sudden onset of symptoms, and general malaise. The variable most associated with contagion was the exposure to a relative with a confirmed diagnosis of COVID-19. Infants and subjects without the influenza vaccine showed an increased risk for respiratory complications. Conclusions: The frequency of positivity in the test was 15% (infants and adolescents represented 64% of the confirmed cases), and the associated factors identified were contact with a confirmed case, sudden onset of symptoms, and chest pain.
MeSH term(s)	Adolescent ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Chest Pain/etiology ; Child ; Child, Preschool ; Clinical Laboratory Techniques ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Cross-Sectional Studies ; Female ; Humans ; Infant ; Male ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Risk Factors ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-10-15
Publishing country	Mexico
Document type	Journal Article
ZDB-ID	730519-9
ISSN	1665-1146 ; 1665-1146 ; 0539-6115 ; 0539-6123
ISSN (online)	1665-1146
ISSN	1665-1146 ; 0539-6115 ; 0539-6123
DOI	10.24875/BMHIM.20000198
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Article: Analysis of the Behaviour of Immunoglobulin G Antibodies in Children and Adults Convalescing From Severe Acute Respiratory Syndrome-Coronavirus-2 Infection.

Márquez-González, Horacio / López-Martínez, Briceida / Parra-Ortega, Israel / de la Rosa-Zamboni, Daniela / Salazar-García, Marcela / Olivar-López, Victor / Klünder-Klünder, Miguel

Frontiers in pediatrics

2021 Volume 9, Page(s) 671831

Abstract: The pandemic caused by SARS CoV-2 (COVID-19) has affected millions of people since 2020. There are clinical differences and in mortality between the adult and paediatric population. Recently, the immune response through the development of antibodies has ... ...

Abstract	The pandemic caused by SARS CoV-2 (COVID-19) has affected millions of people since 2020. There are clinical differences and in mortality between the adult and paediatric population. Recently, the immune response through the development of antibodies has gained relevance due to the risk of reinfection and vaccines' development.
Language	English
Publishing date	2021-08-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711999-3
ISSN	2296-2360
ISSN	2296-2360
DOI	10.3389/fped.2021.671831
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Article ; Online: Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution Melting (SA-HRM) Analysis.

Diaz-Garcia, Hector / Guzmán-Ortiz, Ana L / Angeles-Floriano, Tania / Parra-Ortega, Israel / López-Martínez, Briceida / Martínez-Saucedo, Mirna / Aquino-Jarquin, Guillermo / Sánchez-Urbina, Rocío / Quezada, Hector / Granados-Riveron, Javier T

Genes

2021 Volume 12, Issue 4

Abstract: The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave ... ...

Abstract	The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave differently in terms of regional distribution and other biological features. Next-generation sequencing (NGS) approaches are used to classify the sequence variants in viruses from individual human patients. However, the cost and relative scarcity of NGS equipment and expertise in developing countries prevent studies aimed to associate specific clades and variants to clinical features and outcomes in such territories. As of March 2021, the GR clade and its derivatives, including the B.1.1.7 and B.1.1.28 variants, predominate worldwide. We implemented the post-PCR small-amplicon high-resolution melting analysis to genotype SARS-CoV-2 viruses isolated from the saliva of individual patients. This procedure was able to clearly distinguish two groups of samples of SARS-CoV-2-positive samples predicted, according to their melting profiles, to contain GR and non-GR viruses. This grouping of the samples was validated by means of amplification-refractory mutation system (ARMS) assay as well as Sanger sequencing.
MeSH term(s)	COVID-19/virology ; Genotyping Techniques/methods ; High-Throughput Nucleotide Sequencing ; Humans ; Mutation ; Nucleic Acid Denaturation ; RNA, Viral/isolation & purification ; SARS-CoV-2/genetics
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-04-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes12040531
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