Article ; Online: Neuroprotective effect of He-Ying-Qing-Re formula on retinal ganglion cell in diabetic retinopathy.
2017 Volume 214, Page(s) 179–189
Abstract: Ethnopharmacology relevance: He-Ying-Qing-Re Formula (HF) was empirically modified from Si-Miao ...
| Abstract | Ethnopharmacology relevance: He-Ying-Qing-Re Formula (HF) was empirically modified from Si-Miao-Yong-An Decoction (SD), which was recorded in the literature of Divine Doctor's Secret Transmission, and has been utilized for centuries to treat vasculopathy through clearing heat and accelerating bloodstream. HF has been used as an effective holistic treatment of diabetic retinopathy (DR) for decades and experimentally reported to ameliorate retinal condition in diabetic mice. Aim of the study: Our study aims to investigate the effect of HF in preventing sustained hyperglycemia and hyperlipidemia-associated retinal ganglion cell (RGC) cell death and its possible mechanism. Materials and methods: Chromatographic fingerprint of HF was obtained upon the UPLC-based analytic system; Diabetic retinopathy was established in streptozotocin (STZ) injection-induced hyperglycemic mice; Alterations of retinal structure was assayed by H&E staining. Expression of PSD-95 and CHOP in retinae was assessed by immunofluorescence; RGC cell line (mRGC) was used for in vitro study. Cell death was analyzed by flow cytometry; Intracellular reactive oxygen species (ROS) was measured by 2',7'-dichlorofluorescin diacetate (DCFDA); Apoptosis-related proteins and signaling were monitored with immunoblotting and colorimetric assay. Results: Chlorogenic acid, ferulic acid, and rutin were identified in HF. HF attenuates the loss of RGCs, thinning of inner retinal layers in diabetic mice. Furthermore, expressions of Brn3a and PSD-95 were restored while CHOP level was downregulated upon HF treatment. In vitro study, HF alleviates H Conclusion: HF administration was a valid therapeutic approach for DR treatment, oriented at the blockade of endoplasmic reticulum- and mitochondria-dependent oxidative stress-induced retinal neurodegeneration including RGC apoptosis. |
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| MeSH term(s) | Activating Transcription Factor 4/metabolism ; Animals ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Apoptosis Regulatory Proteins/metabolism ; Blood Glucose/drug effects ; Blood Glucose/metabolism ; Cell Line ; Cytoprotection ; Diabetes Mellitus, Experimental/blood ; Diabetes Mellitus, Experimental/complications ; Diabetes Mellitus, Experimental/drug therapy ; Diabetic Retinopathy/blood ; Diabetic Retinopathy/etiology ; Diabetic Retinopathy/pathology ; Diabetic Retinopathy/prevention & control ; Disks Large Homolog 4 Protein/metabolism ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/pharmacology ; Endoplasmic Reticulum Stress/drug effects ; Hydrogen Peroxide/toxicity ; Hypoglycemic Agents/pharmacology ; Male ; Mice, Inbred C57BL ; Mitochondria/drug effects ; Mitochondria/metabolism ; Mitochondria/pathology ; Neuroprotective Agents/pharmacology ; Oxidative Stress/drug effects ; Reactive Oxygen Species/metabolism ; Retinal Ganglion Cells/drug effects ; Retinal Ganglion Cells/metabolism ; Retinal Ganglion Cells/pathology ; Signal Transduction/drug effects ; Time Factors ; Transcription Factor CHOP/metabolism |
| Chemical Substances | Antioxidants ; Apoptosis Regulatory Proteins ; Atf4 protein, mouse ; Blood Glucose ; Ddit3 protein, mouse ; Disks Large Homolog 4 Protein ; Dlg4 protein, mouse ; Drugs, Chinese Herbal ; Hypoglycemic Agents ; Neuroprotective Agents ; Reactive Oxygen Species ; he-ying-qing-re ; Activating Transcription Factor 4 (145891-90-3) ; Transcription Factor CHOP (147336-12-7) ; Hydrogen Peroxide (BBX060AN9V) |
| Language | English |
| Publishing date | 2017-12-15 |
| Publishing country | Ireland |
| Document type | Journal Article |
| ZDB-ID | 134511-4 |
| ISSN | 1872-7573 ; 0378-8741 |
| ISSN (online) | 1872-7573 |
| ISSN | 0378-8741 |
| DOI | 10.1016/j.jep.2017.12.018 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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