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  1. Article: Letter to the Editor: Utility of Serial Patient Reported Outcomes Measurement Information System (PROMIS®) Physical Function Assessment in a Geriatrics Outpatient Clinic.

    Newmeyer, N / Zhong, L / Cheslock, M / Sison, S D M / Raman, V / Whyman, J D / Kim, D H

    The Journal of frailty & aging

    2023  Volume 12, Issue 4, Page(s) 329–330

    MeSH term(s) Humans ; Aged ; Patient Reported Outcome Measures ; Ambulatory Care Facilities ; Information Systems
    Language English
    Publishing date 2023-11-27
    Publishing country France
    Document type Letter ; Research Support, N.I.H., Extramural
    ZDB-ID 2856228-8
    ISSN 2273-4309 ; 2260-1341
    ISSN (online) 2273-4309
    ISSN 2260-1341
    DOI 10.14283/jfa.2023.32
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Telomere length and telomerase activity delineate distinctive replicative features of the B-CLL subgroups defined by immunoglobulin V gene mutations.

    Damle, Rajendra N / Batliwalla, Franak M / Ghiotto, Fabio / Valetto, Angelo / Albesiano, Emilia / Sison, Cristina / Allen, Steven L / Kolitz, Jonathan / Vinciguerra, Vincent P / Kudalkar, Prasad / Wasil, Tarun / Rai, Kanti R / Ferrarini, Manlio / Gregersen, Peter K / Chiorazzi, Nicholas

    Blood

    2003  Volume 103, Issue 2, Page(s) 375–382

    Abstract: ... Leukemic cells of the immunoglobulin V gene mutation subgroups differ in telomere length and telomerase ...

    Abstract Patients with B-cell chronic lymphocytic leukemia (B-CLL) segregate into subgroups with very different survival times. Because clinical observations suggest that leukemic cells accumulate at different rates, we measured telomere length and telomerase activity in B-CLL cells to distinguish differences in cellular replication. Our data indicate that the telomeres of B-CLL cells are shorter than telomeres of B cells from healthy subjects, indicating that the leukemic cells have a prolonged proliferative history. Leukemic cells of the immunoglobulin V gene mutation subgroups differ in telomere length and telomerase activity. B lymphocytes from the subgroup with poor outcome and with limited IgV gene mutations have uniformly shorter telomeres and more telomerase activity than those from the subgroup with better outcome and with considerable mutations. Differences in telomere length appear to largely reflect the proliferative histories of precursors of the leukemic cells, although differences in cell division, masked by the action of telomerase, cannot be excluded. These results may provide insight into the stages of maturation and the activation pathways of the cells that give rise to B-CLL. In addition, they reinforce the concept that B-CLL is not simply an accumulative disease of slowly dividing B lymphocytes but possibly one of B cells with extensive proliferative histories.
    MeSH term(s) Antigens, Differentiation, B-Lymphocyte ; B-Lymphocytes/enzymology ; B-Lymphocytes/immunology ; Genes, Immunoglobulin ; Humans ; Immunoglobulin Variable Region/genetics ; Kinetics ; Leukemia, Lymphocytic, Chronic, B-Cell/blood ; Leukemia, Lymphocytic, Chronic, B-Cell/enzymology ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; Leukocytes, Mononuclear/enzymology ; Lymphocyte Activation ; Mutation ; Neutrophils/enzymology ; Neutrophils/immunology ; Telomerase/metabolism ; Telomere/enzymology ; Telomere/ultrastructure ; Time Factors
    Chemical Substances Antigens, Differentiation, B-Lymphocyte ; Immunoglobulin Variable Region ; Telomerase (EC 2.7.7.49)
    Language English
    Publishing date 2003-09-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood-2003-04-1345
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  3. Article ; Online: Genome-wide estrogen receptor-α binding and action in human endometrial stromal cells.

    Yilmaz, Bahar D / Sison, Christia A M / Yildiz, Sule / Miyazaki, Kaoru / Coon V, John / Yin, Ping / Bulun, Serdar E

    F&S science

    2020  Volume 1, Issue 1, Page(s) 59–66

    Abstract: Objective: To investigate the gene targets of estradiol (E2)-estrogen receptor-α (ESR1) in human endometrial stromal cells.: Design: Basic science.: Setting: University research center.: Patient(s): Premenopausal women with or without ... ...

    Abstract Objective: To investigate the gene targets of estradiol (E2)-estrogen receptor-α (ESR1) in human endometrial stromal cells.
    Design: Basic science.
    Setting: University research center.
    Patient(s): Premenopausal women with or without endometriosis.
    Intervention(s): Primary cultures of human endometrial stromal cells from healthy endometrium, with or without small-interfering RNA (siRNA) knockdown of ESR1 expression, were treated with E2 or vehicle control.
    Main outcome measure(s): Genome-wide RNA expression by RNA sequencing was compared in endometrial stromal cells with or without siRNA knockdown of ESR1 in the presence or absence of E2. Genome-wide recruitment of ESR1 to chromatin was assessed by chromatin immunoprecipitation sequencing. Gene expression by real-time qualitative polymerase chain reaction of a potential E2-ESR1 target gene was determined in endometrial stromal cells and endometriotic stromal cells.
    Result(s): We identified several important pathways that are dependent on E2-ESR1 signaling in endometrial stromal cells, including progesterone signaling, cell-matrix adhesion, and cytoskeleton rearrangement, as well as paracrine signaling by members of the fibroblast growth factor family. We detected a total of 709 ESR1 target sites on chromatin. By integrating data on genome-wide transcriptomic changes and E2-ESR1 binding sites, we identified inositol polyphosphate phosphatase type II (INPP4B) as a candidate E2-mediated suppressor of proliferation in healthy endometrial cells. INPP4B was downregulated in endometriosis-derived stromal cells.
    Conclusion(s): E2-ESR1 activates genes involved in human endometrial stromal cell cycle regulation, progesterone response, and production of stromal growth factors. Understanding the direct role of estrogen on the endometrial stroma and identifying downstream targets of E2-ESR1 can inform the development of targeted therapies for endometriosis and diminished endometrial receptivity.
    Language English
    Publishing date 2020-05-21
    Publishing country United States
    Document type Journal Article
    ISSN 2666-335X
    ISSN (online) 2666-335X
    DOI 10.1016/j.xfss.2020.06.002
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  4. Article ; Online: GATA2 and Progesterone Receptor Interaction in Endometrial Stromal Cells Undergoing Decidualization.

    Kohlmeier, Amanda / Sison, Christia Angela M / Yilmaz, Bahar D / Coon V, John S / Dyson, Matthew T / Bulun, Serdar E

    Endocrinology

    2020  Volume 161, Issue 6

    Abstract: The transcription factor GATA2 is important for endometrial stromal cell decidualization in early pregnancy. Progesterone receptor (PGR) is also critical during decidualization but its interaction with GATA2 in regulating genes and pathways necessary for ...

    Abstract The transcription factor GATA2 is important for endometrial stromal cell decidualization in early pregnancy. Progesterone receptor (PGR) is also critical during decidualization but its interaction with GATA2 in regulating genes and pathways necessary for decidualization in human endometrium are unclear. RNA-sequencing (RNA-seq) was performed to compare gene expression profiles (n = 3), and chromatin immunoprecipitation followed by sequencing (ChIP-seq) using an antibody against GATA2 (n = 2) was performed to examine binding to target genes in human endometrial stromal cells undergoing in vitro decidualization (IVD including estrogen, progestin, and 3',5'-cyclic AMP analogue) or vehicle treatment. We identified 1232 differentially expressed genes (DEGs) in IVD vs vehicle. GATA2 cistrome in IVD-treated cells was enriched with motifs for GATA, ATF, and JUN, and gene ontology analysis of GATA2 cistrome revealed pathways that regulate cholesterol storage, p38 mitogen-activated protein kinase, and the c-Jun N-terminal kinase cascades. Integration of RNA-seq and ChIP-seq data revealed that the PGR motif is highly enriched at GATA2 binding regions surrounding upregulated genes in IVD-treated cells. The integration of a mined public PGR cistrome in IVD-treated human endometrial cells with our GATA2 cistrome showed that GATA2 binding was significantly enhanced at PGR-binding regions in IVD vs vehicle. Interrogating 2 separate ChIP-seq data sets together with RNA-seq revealed integration of GATA2 and PGR action to coregulate biologic processes during decidualization of human endometrial stromal cells, specifically via WNT activation and stem cell differentiation pathways. These findings reveal the key pathways that are coactivated by GATA2 and PGR that may be therapeutic targets for supporting implantation and early pregnancy.
    MeSH term(s) Adult ; Cells, Cultured ; Decidua/metabolism ; Endometrium/metabolism ; Estrogens/metabolism ; Female ; GATA2 Transcription Factor/genetics ; GATA2 Transcription Factor/metabolism ; Humans ; Middle Aged ; Progestins/metabolism ; Protein Binding ; Receptors, Progesterone/genetics ; Receptors, Progesterone/metabolism ; Stromal Cells/metabolism ; Transcriptome
    Chemical Substances Estrogens ; GATA2 Transcription Factor ; GATA2 protein, human ; Progestins ; Receptors, Progesterone
    Language English
    Publishing date 2020-04-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 427856-2
    ISSN 1945-7170 ; 0013-7227
    ISSN (online) 1945-7170
    ISSN 0013-7227
    DOI 10.1210/endocr/bqaa070
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  5. Article ; Online: High burden of soil-transmitted helminth infections, schistosomiasis, undernutrition, and poor sanitation in two Typhoon Haiyan-stricken provinces in Eastern Philippines.

    Belizario, Vicente / Delos Trinos, John Paul Caesar Robles / Sison, Olivia / Miranda, Esther / Molina, Victorio / Cuayzon, Agnes / Isiderio, Maria Epifania / Delgado, Rodel

    Pathogens and global health

    2021  Volume 115, Issue 6, Page(s) 412–422

    Abstract: This study aimed to describe: 1) soil-transmitted helminthiasis (STH) and schistosomiasis prevalence and intensity in preschool-age children (PSAC) and school-age children (SAC), 2) schistosomiasis seroprevalence in SAC, 3) undernutrition prevalence in ... ...

    Abstract This study aimed to describe: 1) soil-transmitted helminthiasis (STH) and schistosomiasis prevalence and intensity in preschool-age children (PSAC) and school-age children (SAC), 2) schistosomiasis seroprevalence in SAC, 3) undernutrition prevalence in SAC, 4) sanitary toilet coverage in households, and 5) association between STH,
    MeSH term(s) Animals ; Child ; Child, Preschool ; Cross-Sectional Studies ; Cyclonic Storms ; Feces ; Helminthiasis/epidemiology ; Helminths ; Humans ; Malnutrition/epidemiology ; Philippines/epidemiology ; Prevalence ; Sanitation ; Schistosomiasis/epidemiology ; Seroepidemiologic Studies ; Soil
    Chemical Substances Soil
    Language English
    Publishing date 2021-05-06
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2625162-0
    ISSN 2047-7732 ; 2047-7724
    ISSN (online) 2047-7732
    ISSN 2047-7724
    DOI 10.1080/20477724.2021.1920777
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  6. Article ; Online: Ensuring fidelity: key elements to consider in disseminating a diabetes telemanagement program for underserved Hispanic/Latinos living with type 2 diabetes.

    Martinez, Sabrina / Nouryan, Christian N / Williams, Myia S / Patel, Vidhi H / Barbero, Paulina / Correa Gomez, Valeria / Marino, Jose / Goris, Nicole / Cigaran, Edgardo / Granville, Dilcia / Murray, Lawrence F / Harris, Yael T / Myers, Alyson / Guzman, Josephine / Makaryus, Amgad N / McFarlane, Samy I / Zeltser, Roman / Pena, Maria / Sison, Cristina /
    Lesser, Martin L / Kline, Myriam / DiClemente, Ralph Joseph / Pekmezaris, Renee

    Frontiers in clinical diabetes and healthcare

    2024  Volume 5, Page(s) 1328993

    Abstract: Background: The Hispanic/Latino population has greater risk (estimated >50%) of developing type 2 diabetes (T2D) and developing it at a younger age. The American Diabetes Association estimates costs of diagnosed diabetes in 2017 was $327 billion; with ... ...

    Abstract Background: The Hispanic/Latino population has greater risk (estimated >50%) of developing type 2 diabetes (T2D) and developing it at a younger age. The American Diabetes Association estimates costs of diagnosed diabetes in 2017 was $327 billion; with medical costs 2.3x higher than patients without diabetes. The purpose of this manuscript is to describe the methodology utilized in a randomized controlled trial aimed at evaluating the efficacy of a diabetes telemanagement (DTM) program for Hispanic/Latino patients with T2D. The intent is to provide information for future investigators to ensure that this study can be accurately replicated.
    Methods: This study was a randomized controlled trial with 240 participants. Eligible patients (Hispanic/Latino, aged 18+, living with T2D) were randomized to Comprehensive Outpatient Management (COM) or DTM. DTM was comprised of usual care, including routine clinic visits every three months, as well as: Biometrics (a tablet, blood glucose meter, blood pressure monitor, and scale); Weekly Video Visits (facilitated in the patient's preferred language); and Educational Videos (including culturally congruent diabetes self-management education and quizzes). COM consisted of usual care including routine clinic visits every three months. For this study, COM patients received a glucometer, glucose test strips, and lancets. Establishing a therapeutic nurse-patient relationship was a fundamental component of our study for both groups. First contact (post-enrollment) centered on ensuring that patients and caregivers understood the program, building trust and rapport, creating a non-judgmental environment, determining language preference, and establishing scheduling availability (including evenings and weekends). DTM were provided with a tablet which allowed for self-paced education through videos and weekly video visits. The research team and Community Advisory Board identified appropriate educational video content, which was incorporated in diabetes educational topics. Video visits allowed us to assess patient involvement, motivation, and nonverbal communication. Communicating in Spanish, and awareness of diverse Hispanic/Latino backgrounds was critical, as using relevant and commonly-used terms can increase adherence and improve outcomes. Shared decision-making was encouraged to make realistic health care choices.
    Conclusion: Key elements discussed above provide a framework for future dissemination of an evidence-based DTM intervention to meet the needs of underserved Hispanic/Latino people living with T2D.
    Language English
    Publishing date 2024-02-16
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6616
    ISSN (online) 2673-6616
    DOI 10.3389/fcdhc.2024.1328993
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  7. Article ; Online: The effect of increased chewing strokes on the DeMeester score.

    Arya, V / Agarwal, S / Singh, S / Sison, C / Gupta, K A

    Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus

    2017  Volume 30, Issue 5, Page(s) 1–5

    Abstract: Saliva is known to be protective for esophageal mucosa. Increased chewing strokes result in a quantitative and qualitative enhancement of saliva. Reduction in the amount of saliva produced results in an increased incidence of gastroesophageal reflux ... ...

    Abstract Saliva is known to be protective for esophageal mucosa. Increased chewing strokes result in a quantitative and qualitative enhancement of saliva. Reduction in the amount of saliva produced results in an increased incidence of gastroesophageal reflux disease (GERD), which can be objectively measured by the DeMeester score. The impact of increased chewing strokes on the DeMeester score remains largely unknown, thus this study aimed to find out their impact on the value of the DeMeester score and its individual components.The effect of increased chewing strokes on the DeMeester score was investigated in 12 subjects (5 male and 7 female) who were diagnosed with GERD. All subjects underwent a 48-hour pH monitoring using the Bravo® pH capsule. All the patients chewed their food 20 times more on Day 2 as compared to Day 1. The data were analyzed for change in the DeMeester score and its individual components in 2 days.In patients with GERD (DeMeester score > 14.72 on Day 1), the number of long refluxes (>5 minutes) on Day 2 (mean = 3.2, SD = 2.3) was significantly lower than on Day 1 (mean = 6.4, SD = 2.7); Z = -2.032, p = 0.04. Though, the DeMeester score and its other individual parameters decreased on Day 2, they were not statistically significant.In patients with GERD, increased chewing strokes lead to a decrease in the number of long reflux episodes. Though there is a decrease in the DeMeester score and its other individual components, larger randomized controlled studies are required to reach statistical significance.
    MeSH term(s) Adult ; Esophageal pH Monitoring ; Female ; Gastroesophageal Reflux/diagnosis ; Gastroesophageal Reflux/physiopathology ; Humans ; Male ; Mastication/physiology ; Middle Aged ; Saliva/secretion ; Severity of Illness Index ; Young Adult
    Language English
    Publishing date 2017-05-01
    Publishing country United States
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 639470-x
    ISSN 1442-2050 ; 1120-8694
    ISSN (online) 1442-2050
    ISSN 1120-8694
    DOI 10.1093/dote/dow016
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  8. Article ; Online: Feasibility of implementing a telephone-based frailty assessment.

    Sison, Stephanie Denise M / Newmeyer, Natalie / Arias, Karla Tejada / Schoenburg, Racheli / Fonseca Valencia, Carolina / Cheslock, Megan / Raman, Vaishnavi / Driver, Jane A / Kim, Dae Hyun

    Journal of the American Geriatrics Society

    2022  Volume 70, Issue 12, Page(s) 3610–3619

    Abstract: Background: Despite the growing literature on the importance of identifying and managing frailty, its assessment has been limited in clinical settings. With the goal of integrating frailty assessment into routine clinical practice, this quality ... ...

    Abstract Background: Despite the growing literature on the importance of identifying and managing frailty, its assessment has been limited in clinical settings. With the goal of integrating frailty assessment into routine clinical practice, this quality improvement project aimed to determine the feasibility, acceptability, and utility of administering a telephone-based frailty assessment.
    Methods: Between 9/2020 and 6/2021, we identified 169 established patients with serious illnesses in an academic primary care-geriatric clinic. Patients were contacted via telephone, and their current medical, functional, nutritional, cognitive, and mood statuses were assessed using validated screening tools. A deficit-accumulation frailty score was then calculated using an electronic medical record-based frailty index calculator and standardized documentation with recommendations was generated for providers. The primary outcome was feasibility, measured as the proportion of patients successfully assessed. Secondary outcomes included completion rates of each domain, administration time, providers' perception, and clinical utility of the assessment.
    Results: A total of 139 (82.2%) patients, mean age of 82 years, 63.3% frail were successfully assessed. Of the 139 assessments, medical and functional domains were completed for all, while nutrition, mood, and cognition were completed by 88.5% (n = 123), 68.3% (n = 95), and 59.7% (n = 83) of the time, respectively. Conducting the full assessment took an average (standard deviation) time of 26.1 (7.3) minutes. Without the cognitive and mood domain, assessment took an average of 15.7 (7.5) minutes. Patients' providers found the information from the assessment helpful in evaluating and managing their patients. Care plans of 51.8% and 65.0% of patients who had mobility and mind issues, respectively, addressed these domains within 30 days after the assessment.
    Conclusion: Implementation of the telephone-based frailty assessment is feasible, acceptable, and has the potential to influence the care plans of older adults. This work demonstrated how frailty assessment can be integrated with the outpatient setting.
    MeSH term(s) Humans ; Aged ; Aged, 80 and over ; Frailty/diagnosis ; Frailty/psychology ; Frail Elderly/psychology ; Feasibility Studies ; Quality Improvement ; Telephone ; Geriatric Assessment
    Language English
    Publishing date 2022-09-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80363-7
    ISSN 1532-5415 ; 0002-8614
    ISSN (online) 1532-5415
    ISSN 0002-8614
    DOI 10.1111/jgs.18031
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  9. Article ; Online: Association of anticipated HIV testing stigma and provider mistrust on preference for HIV self-testing among cisgender men who have sex with men in the Philippines.

    Sison, Olivia T / Baja, Emmanuel S / Bermudez, Amiel Nazer C / Quilantang, Ma Irene N / Dalmacion, Godofreda V / Guevara, Ernest Genesis / Garces-Bacsal, Rhoda Myra / Hemingway, Charlotte / Taegtmeyer, Miriam / Operario, Don / Biello, Katie B

    BMC public health

    2022  Volume 22, Issue 1, Page(s) 2362

    Abstract: Background: New HIV infections in the Philippines are increasing at an alarming rate. However, over three quarters of men who have sex with men (MSM) have never been tested for HIV. HIV self-testing (HIVST) may increase overall testing rates by removing ...

    Abstract Background: New HIV infections in the Philippines are increasing at an alarming rate. However, over three quarters of men who have sex with men (MSM) have never been tested for HIV. HIV self-testing (HIVST) may increase overall testing rates by removing barriers, particularly fear of stigmatization and mistrust of providers. This study aimed to determine if these factors are associated with preference for HIVST among Filipino cisgender MSM (cis-MSM), and whether there is an interaction between anticipated HIV testing stigma and provider mistrust on preference for HIVST.
    Methods: We conducted secondary analysis of a one-time survey of 803 cis-MSM who were recruited using purposive sampling from online MSM dating sites and MSM-themed bar locations in Metro Manila, Philippines. Summary statistics were computed to describe participant characteristics. Multivariable modified Poisson regression analyses were conducted to determine if anticipated HIV testing stigma and provider mistrust were associated with preference for HIVST among cis-MSM. Other variables such as age, education, monthly income, relationship status, HIV serostatus, and knowing where to get HIV testing were the minimal sufficient adjustment set in the analyses.
    Results: Average age of participants was 28.6 years (SD = 8.0); most had received college degrees (73%) and were employed (80%). Most respondents (81%) preferred facility-based testing, while 19% preferred HIVST. A high percentage of participants reported anticipated HIV testing stigma (66%) and provider mistrust (44%). Anticipated HIV testing stigma (aPR = 1.51; 95% CI = 1.01-2.25, p = 0.046) and provider mistrust (aPR = 1.49; 95% CI = 1.07-2.09, p = 0.020) were independently associated with a preference for HIVST. There was a positive, additive interaction between provider mistrust and anticipated HIV testing stigma on preference for HIVST (RERI = 1.13, 95% CI: 0.20-2.06; p = 0.017), indicating that the association between anticipated HIV testing stigma and preference for HIVST is greater among those with provider mistrust compared to those without provider mistrust.
    Conclusions: HIVST should be offered as a supplement to traditional facility-based HIV testing services in the Philippines to expand testing and reach individuals who may not undergo testing due to anticipated HIV testing stigma and provider mistrust.
    MeSH term(s) Male ; Humans ; Adult ; HIV ; Homosexuality, Male ; Self-Testing ; HIV Infections/diagnosis ; Philippines ; Sexual and Gender Minorities ; HIV Testing
    Language English
    Publishing date 2022-12-16
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041338-5
    ISSN 1471-2458 ; 1471-2458
    ISSN (online) 1471-2458
    ISSN 1471-2458
    DOI 10.1186/s12889-022-14834-x
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  10. Article ; Online: Evaluation of loop-mediated isothermal amplification assay and enzyme-linked immunosorbent assay in detecting Schistosoma japonicum in Siargao Island, Surigao del Norte, the Philippines.

    Belizario, Vicente Y / Delos Trinos, John Paul Caesar R / Sison, Olivia T / Destura, Raul V / Medina, John Robert / Gigataras, April Joy E / Petronio-Santos, Joy Ann G / Abarientos, Adrian B

    Acta tropica

    2022  Volume 228, Page(s) 106306

    Abstract: Objectives: This study aimed to describe the prevalence and seroprevalence of schistosomiasis in Siargao Island, Surigao del Norte and to compare the performance of enzyme-linked immunosorbent assay antibody test (ELISA Ab) and loop-mediated isothermal ... ...

    Abstract Objectives: This study aimed to describe the prevalence and seroprevalence of schistosomiasis in Siargao Island, Surigao del Norte and to compare the performance of enzyme-linked immunosorbent assay antibody test (ELISA Ab) and loop-mediated isothermal amplification assay (LAMP) for diagnosis of schistosomiasis.
    Methods: The study was conducted in selected barangays (villages) in five municipalities in Siargao Island, Surigao del Norte and included school-age children (SAC) who submitted stool and blood samples. Stool samples were examined using the Kato-Katz technique. Blood samples were collected through venipuncture. The stool samples and the blood samples collected were tested using LAMP assay and polymerase chain reaction (PCR). The blood samples were examined using ELISA Ab. Diagnostic performance of LAMP assay using stool specimen was evaluated using Kato-Katz technique and PCR assay as the composite reference standard, while PCR assay was used as the reference standard to evaluate LAMP assay and ELISA Ab using blood specimens.
    Results: A total of 417 stool samples from SAC were examined. The prevalence of schistosomiasis and moderate-heavy intensity (MHI) schistosomiasis were 3.8% and 1.4%, respectively. Schistosomiasis and soil-transmitted helminthiases (STH) coinfection prevalence were 2.6%. A total of 425 blood samples were examined using ELISA Ab. Seroprevalence was 61.6%. The municipality of San Isidro had the highest seroprevalence at 84.8%, while Burgos had the lowest seroprevalence at 48.5%.LAMP assay had higher sensitivity and positive predictive value but lower specificity when using stool than when using blood samples. Its negative predictive value was similar regardless of the specimen used. ELISA Ab has higher sensitivity and negative predictive value than LAMP assay although it has lower specificity and positive predictive value. This may be due to ELISA Ab measuring Schistosoma exposure and is thus unable to distinguish past from active infection.
    Conclusions: Schistosomiasis remains a public health concern in Siargao Island, Surigao del Norte. The locally developed LAMP assay offers a simpler diagnostic test for schistosomiasis compared with PCR, while minimizing the risk of misdiagnosis compared with Kato-Katz technique. It could serve as a point of care diagnostics for schistosomiasis. ELISA Ab is more useful in surveillance particularly in low-endemicity areas where determination of exposure is more important than differentiating past from active infection. ELISA Ab may be helpful in the clinical setting when coupled with the expertise of a physician who is familiar with schistosomiasis.
    MeSH term(s) Animals ; Child ; Enzyme-Linked Immunosorbent Assay ; Feces ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Philippines/epidemiology ; Prevalence ; Schistosoma japonicum/genetics ; Sensitivity and Specificity ; Seroepidemiologic Studies
    Language English
    Publishing date 2022-01-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2022.106306
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