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Article: Anti-osteoporotic effects of Yi Mai Jian on bone metabolism of ovariectomized rats.

Shi, Bin / Lin, Che-Chun / Lee, Chia-Jung / Ning, De-Shan / Lin, Chao-Chi / Zhao, Hong-Wei / Yang, Chang-Syun / Deng, Shun-Xin / Chiu, Yung-Jia / Wang, Ching-Chiung

Frontiers in pharmacology

2024 Volume 15, Page(s) 1326415

Abstract: Yi Mai Jian herbal formula (YMJ) is formulated with Eucommiae Folium, Astragali Radix ...

Abstract	Yi Mai Jian herbal formula (YMJ) is formulated with Eucommiae Folium, Astragali Radix, Ligustri Lucidi Fructus, and Elaeagnus Fructus to improve bone function in traditional Chinese medicine. The anti-osteoporotic effects of YMJ in bone metabolism were evaluated in ovariectomized (OVX) rats. The skeletal structure of the femur and vertebrae was analyzed after treating OVX rats with YMJ for 114 days. The results showed that YMJ significantly increased the bone mineral density (BMD) and trabecular number (Tb. N) of the femur and 5th lumbar vertebrae and reduced trabecular separation (Tb. Sp). Moreover, trabecular bone volume/total tissue volume (BV/TV), bone stiffness, and maximum femur load were significantly increased. The serum concentrations of NTX1 and PYD were significantly decreased. According to these results, YMJ could ameliorate osteoporosis in ovariectomized rats. Eucommiae Folium and Elaeagnus Fructus inhibited osteoclast differentiation, Ligustri Lucidi Fructus inhibited calcium reabsorption, Astragali Radix stimulated osteoblast proliferation, and Astragali Radix and Eucommiae Folium stimulated mineralization. Therefore, the combination of the four herbs into one formula, YMJ, could alleviate bone remodeling caused by low estrogen levels. We suggest that YMJ could be a healthy food candidate for preventing post-menopausal osteoporosis.
Language	English
Publishing date	2024-03-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2024.1326415
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Article ; Online: Yi-Qi-Huo-Xue decoction alleviates intracerebral hemorrhage injury through inhibiting neuronal autophagy of ipsilateral cortex via BDNF/TrkB pathway.

Han, Dan / Chang, Xinyue / Xu, Dan / Shen, Jizhong / Fan, Ali / Wang, Meihua / Li, Dingran / Chen, Xiangkai / Wang, Cheng / Wu, Yi / Yang, Zhaocong / Li, Jian / Wang, Siliang

Phytomedicine : international journal of phytotherapy and phytopharmacology

2024 Volume 128, Page(s) 155438

Abstract: Background: Yi-Qi-Huo-Xue Decoction (YQHXD), a traditional Chinese medicine formula, has ...

Abstract	Background: Yi-Qi-Huo-Xue Decoction (YQHXD), a traditional Chinese medicine formula, has demonstrated efficacy in the clinical treatment of intracerebral hemorrhage (ICH) for over a decade. Nevertheless, the precise pharmacotherapeutic compounds of YQHXD capable of penetrating into cerebral tissue and the pharmacological underpinnings of YQHXD remain ambiguous. Methods: The active components of YQHXD in rat brains was analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The potential targets, pathways and biological progresses of YQHXD ameliorating ICH induced injury was predicted by network pharmacology. Moreover, collagenase-induced ICH rat model, primary cortex neurons exposed to hemin and molecular docking were applied to validate the molecular mechanisms of YQHXD. Results: Eleven active components of YQHXD were identified within the brains. Employing the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, our investigation concentrated on the roles of autophagy and the BDNF/TrkB signaling pathway in the pharmacological context. The pharmacological results revealed that YQHXD alleviated neurological dysfunction, brain water content, brain swelling, and pathological injury caused by ICH. Meanwhile, YQHXD inhibited autophagy influx and autophagosome in vivo, and regulated cortex neuronal autophagy and TrkB/BDNF pathway both in vivo and in vitro. Subsequently, N-acetyl serotonin (NAS), a selective TrkB agonist, was employed to corroborate the significance of the BDNF/TrkB pathway in this process. The combination of NAS and YQHXD did not further enhance the protective efficacy of YQHXD in ICH rats. Additionally, outcomes of molecular docking analysis revealed that nine compounds of YQHXD exhibited potential regulatory effects on TrkB. Conclusions: Ipsilateral neuronal autophagy and BDNF/TrkB pathway were activated 72 h after ICH. YQHXD effectively resisted injury induced by ICH, which was related with suppression of ipsilateral neuronal autophagy via BDNF/TrkB pathway. This study provides novel insights into the therapeutic mechanisms of traditional Chinese medicine in the context of ICH treatment.
Language	English
Publishing date	2024-02-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2024.155438
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Article: Scapular kinematics and muscle activity during Yi Jin Bang exercises.

Liu, Jinde / Hui, Stanley Sai-Chuen / Yang, Yijian / Liu, Yanhao / Song, Qipeng / Mao, Dewei

Frontiers in physiology

2023 Volume 14, Page(s) 1169092

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2023-06-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2023.1169092
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Article ; Online: Yi Mai granule improve energy supply of endothelial cells in atherosclerosis via miRNA-125a-5p regulating mitochondrial autophagy through Pink1-Mfn2-Parkin pathway.

Kong, De Zhao / Sun, Peng / Lu, Yi / Yang, Ye / Min, Dong Yu / Zheng, Si Cheng / Yang, Yi / Zhang, Zhe / Yang, Guan Lin / Jiang, Jun Wen

Journal of ethnopharmacology

2023 Volume 319, Issue Pt 1, Page(s) 117114

Abstract: Ethnopharmacological relevance: Yi Mai granule (YMG) consists of two classic Chinese medicine ...

Abstract	Ethnopharmacological relevance: Yi Mai granule (YMG) consists of two classic Chinese medicine formulas used to treat cardiovascular disease for centuries. The Pink1-Mfn2-Parkin pathway, a well-recognized mechanism that mediates mitochondrial autophagy, plays a big part in mitochondrial quality control and the maintenance of heart function. However, the effects of YMG on endothelial dysfunction and mitochondrial autophagy remain unknown. Aim of the study: Here, we focused on the therapeutic effects of YMG in improving mitochondrial autophagy and the mechanism of YMG against cardiovascular disease. Materials and methods: In this study, rats were fed high-fat diet (HFD) for 21 weeks and were given high, medium, and low doses of YMG in stomach. The open field test was used to evaluate the rats' behavior. Atherosclerotic plaques, blood lipids, and cytokine levels were measured. Mitochondrial autophagy changes were observed by Transmission electron microscope (TEM). Human umbilical vein endothelial cells (HUVECs) were injured by angiotensinⅡ(AngⅡ) and were given high, medium, and low doses of YMG medicated serum in cell culture medium. Pink1-Mfn2-Parkin expression and miRNA 125a-5p expression were measured by RT-PCR and Western blot. Results: We demonstrated that the atherosclerosis model group tended to exhibit reduced vitality behaviors. We proved that the atherosclerosis model group showed obvious atherosclerotic plaques, endothelial cells destruction, and high level of blood lipid and cytokines (including hs-CRP, ET). Mitochondria were reduced, and mitophagy was inhibited in aortic cells of the model group. MiRNA-125a-5p was up-regulated; at the same time, Pink1-Mfn2-Parkin-mediated mitochondrial autophagy was prevented. We also proved that AngⅡinjured HUVEC showed obviously low mRNA levels of Pink1, Mfn2, and Parkin. Interestingly, we found that miRNA-125a-5p was significantly down regulated in Ang II-induced HUVECs. In addition, miRNA-125a-5p significantly reduced the protective effect of YiMai Granules against Ang II injury. Conclusion: Our finding indicated that Pink1-Mfn2-Parkin-mediated mitochondrial autophagy plays a crucial role in alleviating atherosclerosis. YMG alleviated atherosclerosis by potentially activating mitochondrial autophagy may via miRNA-125a-5p, regulating Pink1-Mfn2-Parkin pathway, and regulating proinflammatory factors, vasoconstriction cytokine, and blood lipids.
MeSH term(s)	Rats ; Humans ; Animals ; Endothelial Cells/metabolism ; Plaque, Atherosclerotic/metabolism ; Cardiovascular Diseases ; Protein Kinases/metabolism ; Autophagy ; Mitochondria ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Atherosclerosis/drug therapy ; Atherosclerosis/genetics ; Atherosclerosis/metabolism ; Cytokines/metabolism ; Lipids/pharmacology ; MicroRNAs/genetics ; MicroRNAs/metabolism
Chemical Substances	Protein Kinases (EC 2.7.-) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; Cytokines ; Lipids ; MicroRNAs
Language	English
Publishing date	2023-09-09
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117114
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Article ; Online: Yi Jin Bang exercise versus usual exercise therapy to treat subacromial pain syndrome: a pilot randomised controlled trial.

Hui, Stanley Sai-Chuen / Liu, Jinde / Yang, Yi-Jian / Wan, James Ho-Pong / Suen, Bonhomme Kwai-Ping

Research in sports medicine (Print)

2022 Volume 31, Issue 6, Page(s) 846–858

Abstract: To evaluate the effect of Yi Jin Bang (YJB) exercise on pain, disability, flexibility, and muscular ...

Abstract	To evaluate the effect of Yi Jin Bang (YJB) exercise on pain, disability, flexibility, and muscular endurance in individuals with subacromial pain syndrome (SAPS). Fifty-four adults with SAPS were randomly allocated into either a YJB (n = 18), a usual exercise therapy (UET; n = 18), or a control group (n = 18). YJB and UET interventions involved 10 weeks of home-based exercise training, with four sessions per week. The control group received no treatment. Outcome measures included Shoulder Pain and Disability Index (SPADI), pain at rest and during activity, flexion and abduction range of motion (ROM), back scratch test, and shoulder abduction endurance test. These measures were assessed at baseline, immediately after a single face-to-face session (acute effects), and after 10 weeks of intervention (chronic effects). For acute effects, significant group-by-time interactions were observed for flexion ROM, abduction ROM, and the back scratch test (all
Language	English
Publishing date	2022-03-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2116093-4
ISSN	1543-8635 ; 1543-8627
ISSN (online)	1543-8635
ISSN	1543-8627
DOI	10.1080/15438627.2022.2052070
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Zs.A 2562: Show issues

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Article: Chinese herbal decoction, Yi-Qi-Jian-Pi formula exerts anti-hepatic fibrosis effects in mouse models of CCl

Yang, Shiyan / Cheng, Yajun / Wang, Xiaolong / Yue, Suyang / Wang, Xi / Tang, Li / Li, Hailun / Zhang, Jie / Xiong, Qingping / Tan, Shanzhong

Heliyon

2024 Volume 10, Issue 5, Page(s) e26129

Abstract: Background: Yi-Qi-Jian-Pi Formula (YQJPF) is a herbal medicine that is used to treat patients ...

Abstract	Background: Yi-Qi-Jian-Pi Formula (YQJPF) is a herbal medicine that is used to treat patients with liver failure. However, scientific evidence supporting the treatment of hepatic fibrosis with YQJPF has not been forthcoming. The present study aimed to determine the mechanisms underlying the anti-fibrotic effects of YQJPF in mouse models of hepatic fibrosis. Methods: Mice were randomly assigned to control, hepatic fibrosis model, silymarin (positive treated), and low-, medium- and high-dose YQJPF (7.5, 15, and 30 g/kg, respectively) groups. Liver function, inflammatory cytokines, and oxygen stress were analyzed using ELISA kits. Sections were histopathologically stained with hematoxylin-eosin, Masson trichrome, and Sirius red. Macrophage polarization was measured by flow cytometry and immunofluorescence. Potential targets of YQJPF against hepatic fibrosis were analyzed by network pharmacology of Chinese herbal compound and the effects of YQJPF on the transforming growth factor-beta (TGF-β)/Suppressor of Mothers against Decapentaplegic family member 3 (Smad3) signaling pathway were assessed using qRT-PCR and immunohistochemical staining. Finally, metagenomics and LC-MS/MS were used to detect the intestinal flora and metabolites of the mice, and an in-depth correlation analysis was performed by spearman correlation analysis. The data were compared by one-way ANOVA and least significant differences (LSDs) or ANOVA-Dunnett's T3 method used when no homogeneity was detected. Results: We induced hepatic fibrosis using CCl Conclusions: YQJPF can reverse liver fibrosis by inhibiting inflammation, suppressing oxidative stress, regulating the immunological response initiated by macrophages, inhibiting TGF-β/Smad3 signaling and regulating intestinal flora homeostasis. Therefore, YQJPF may be included in clinical regimens to treat hepatic fibrosis.
Language	English
Publishing date	2024-02-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e26129
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Article ; Online: Efficacy of Shu-yi-ning-chang decoction on IBS-D: Modulating Nr4a3 pathway to reduce visceral hypersensitivity.

Guo, Yajing / Lu, Qiongqiong / Yang, Xiao-Jun / He, Yuxi / Wu, Yue / Qin, Baijun / Li, Ting / Duan, Min / Liu, Nvping / Wu, Xin / He, Yuanjun

PloS one

2024 Volume 19, Issue 4, Page(s) e0299376

Abstract: Aim of the study: To evaluate the therapeutic effect of SYNC in diarrhea irritable bowel syndrome (IBS-D) and explore its underlying mechanism through transcriptomic sequencing (RNA-Seq).: Materials and methods: A rat model of IBS-D was constructed ... ...

Abstract	Aim of the study: To evaluate the therapeutic effect of SYNC in diarrhea irritable bowel syndrome (IBS-D) and explore its underlying mechanism through transcriptomic sequencing (RNA-Seq). Materials and methods: A rat model of IBS-D was constructed to elucidate the effects of SYNC. Abdominal withdrawal reflex (AWR), fecal water content (FWC), and recording body weight were calculated to assess visceral sensitivity in rats. Histopathological changes in the colon and alterations in mast cell (MC) count were determined. Immunohistochemistry was employed to assess mast cell tryptase (MCT) expression in rat colons. Serum levels of corticotropin-releasing Hormone (CRH), interleukin-6 (IL-6), calcitonin gene-related peptide (CGRP), and 5-hydroxytryptamine (5-HT) were quantified using ELISA. RNA-Seq of colon tissue was performed, followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Western blot analysis was conducted to quantify the expression levels of key proteins in the Nr4a3 pathway in the colon and hypothalamus tissues of rats. Results: SYNC alleviated visceral hypersensitivity and mood disorders in rats with IBS-D. Moreover, it was positively correlated with its dosage and the observed effects, such as the enhancement of the colon's mucosal lining condition and reduction in the number and activation of MCs within the model group. SYNC reduced the expression levels of factors related to the brain-gut axis and inflammatory markers in the bloodstream. RNA-Seq analysis indicated that SYNC down-regulated the expression of Nr4a3 and PI3K. These SYNC-targeted genes primarily played roles in immune regulation and inflammatory responses, correlating with the modulation of Nr4a3 and the PI3K/AKT pathway. Western blot analysis further confirmed SYNC's influence on inflammation-related MC activation by downregulating key proteins in the Nr4a3/PI3K pathway. Conclusions: SYNC inhibited mast cell activation and attenuated visceral hypersensitivity in the colon tissues of IBS-D rats. These effects were mediated by the Nr4a3/PI3K signaling pathway.
MeSH term(s)	Rats ; Animals ; Irritable Bowel Syndrome/pathology ; Rats, Sprague-Dawley ; Phosphatidylinositol 3-Kinases ; Diarrhea ; Corticotropin-Releasing Hormone/metabolism ; DNA-Binding Proteins ; Nerve Tissue Proteins
Chemical Substances	Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Corticotropin-Releasing Hormone (9015-71-8) ; Nr4a3 protein, rat ; DNA-Binding Proteins ; Nerve Tissue Proteins
Language	English
Publishing date	2024-04-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0299376
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Article ; Online: Bu Shen Yi Sui Capsule Promotes Myelin Repair by Modulating the Transformation of A1/A2 Reactive Astrocytes

Zha, Zheng / Liu, Yi-Jiang / Liu, Si-Si / Zhang, Nan / Li, Jun-Ling / Qi, Fang / Jin, Liang-Yun / Xue, Bing / Yang, Tao / Fan, Yong-Ping / Zhao, Hui / Wang, Lei

Oxidative medicine and cellular longevity

2022 Volume 2022, Page(s) 3800004

Abstract: Background/ ... ...

Abstract	Background/Aims
MeSH term(s)	Animals ; Astrocytes/metabolism ; Central Nervous System ; Complement C1q/metabolism ; Complement C1q/pharmacology ; Mice ; Mice, Inbred C57BL ; MicroRNAs/metabolism ; Multiple Sclerosis/drug therapy ; Multiple Sclerosis/metabolism ; Myelin Sheath ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	MicroRNAs ; Tumor Necrosis Factor-alpha ; Complement C1q (80295-33-6)
Language	English
Publishing date	2022-09-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2455981-7
ISSN	1942-0994 ; 1942-0994
ISSN (online)	1942-0994
ISSN	1942-0994
DOI	10.1155/2022/3800004
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Article: Neurotherapy of Yi-Gan-San, a Traditional Herbal Medicine, in an Alzheimer's Disease Model of

Su, Ming-Tsan / Jheng, Yong-Sin / Lu, Chen-Wen / Wu, Wen-Jhen / Yang, Shieh-Yueh / Chuang, Wu-Chang / Lee, Ming-Chung / Wu, Chung-Hsin

Plants (Basel, Switzerland)

2022 Volume 11, Issue 4

Abstract: ... that is related to the abnormal accumulation of amyloid β (Aβ) proteins. Yi-Gan-San (YGS), a traditional ...

Abstract	Alzheimer's disease (AD), a main cause of dementia, is the most common neurodegenerative disease that is related to the abnormal accumulation of amyloid β (Aβ) proteins. Yi-Gan-San (YGS), a traditional herbal medicine, has been used for the management of neurodegenerative disorders and for the treatment of neurosis, insomnia and dementia. The aim of this study was to examine antioxidant capacity and cytotoxicity of YGS treatment by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays in vitro. We explored neuroprotective effects of YGS treatment in alleviating Aβ neurotoxicity of Drosophila melanogaster in vivo by comparing survival rate, climbing index, and Aβ expressions through retinal green fluorescent protein (GFP) expression, highly sensitive immunomagnetic reduction (IMR) and Western blotting assays. In the in vitro study, our results showed that scavenging activities of free radical and SH-SY5Y nerve cell viability were increased significantly (
Language	English
Publishing date	2022-02-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11040572
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Article ; Online: Increasing prevalence of overweight and obesity in Yi farmers and migrants from 2007 to 2015 in China: the Yi migrant study.

Wang, Ye / Pan, Li / Wan, Shaoping / Yi, Huowuli / Yang, Fang / He, Huijing / Li, Zheng / Zhang, Jia / Wang, Xiaoyang / Yong, Zhengping / Shan, Guangliang

BMC public health

2018 Volume 18, Issue 1, Page(s) 659

Abstract: ... and related factors of overweight and obesity in Yi farmers and migrants in southwest China from 2007 ... prevalence of overweight increased in both groups (from 5.54 to 16.65% in Yi farmers, from 27.91 to 33.61 ... in Yi migrants). Standardized prevalence of obesity increased only in Yi farmers (from 0.37 to 3.13 ...

Abstract	Background: With the social development and lifestyle transition, increasing trends of overweight and obesity prevalence are commonly reported worldwide. Data focusing on overweight and obesity trends in rural residents and rural-to-urban migrants in China are limited. This study aims to assess the changes and related factors of overweight and obesity in Yi farmers and migrants in southwest China from 2007 to 2015, and to assess the disparities in prevalence changes. Methods: Pseudo-panel data was obtained from two cross-sectional studies conducted in Sichuan province, southwest China in 2007 and 2015. Standardized prevalence of overweight and obesity were evaluated by direct method using the 2010 national census population as the standard. Prevalence differences of overweight and obesity were calculated in each group and compared between groups to evaluate the disparity in prevalence changes. Generalized linear mixed model was performed to determine factors related to overweight/obesity. Results: Standardized prevalence of overweight increased in both groups (from 5.54 to 16.65% in Yi farmers, from 27.91 to 33.61% in Yi migrants). Standardized prevalence of obesity increased only in Yi farmers (from 0.37 to 3.13%). Prevalence difference of overweight in Yi farmers was higher than that in Yi migrants (11.11 vs. 5.70), but prevalence differences of obesity were not significantly different between Yi farmers and migrants. Conclusions: Prevalence of overweight and obesity in Yi farmers, and prevalence of overweight in Yi migrants increased from 2007 to 2015. Yi farmers were developing overweight at a greater pace than migrants. More attention should be paid to spread health knowledge and encourage healthy lifestyle in Yi people, especially Yi farmers.
MeSH term(s)	Adult ; Agriculture/statistics & numerical data ; China/epidemiology ; Cross-Sectional Studies ; Farmers/statistics & numerical data ; Female ; Health Status ; Humans ; Male ; Middle Aged ; Obesity/epidemiology ; Prevalence ; Rural Population/statistics & numerical data ; Transients and Migrants/statistics & numerical data
Language	English
Publishing date	2018-05-24
Publishing country	England
Document type	Journal Article
ISSN	1471-2458
ISSN (online)	1471-2458
DOI	10.1186/s12889-018-5577-4
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