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Article: Added value of a connected glucose meter for glycorrhachia assessment.

Blairon, Laurent / Tré-Hardy, Marie / Collignon, Sophie / Coenen, François / Beukinga, Ingrid / Cupaiolo, Roberto

2024 Volume 39, Page(s) e00384

Abstract: Objectives: The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.: Design and methods: Intra-assay and inter-assay accuracies were calculated using ...

Abstract	Objectives: The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer. Design and methods: Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform. Results: The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r Conclusions: The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.
Language	English
Publishing date	2024-03-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2834973-8
ISSN	2352-5517
ISSN	2352-5517
DOI	10.1016/j.plabm.2024.e00384
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Article ; Online: Monocytes warning for malaria.

Riahi, Nada / Tré-Hardy, Marie / Hernando, Carmen / Blairon, Laurent / Cupaiolo, Roberto / Beukinga, Ingrid

British journal of haematology

2023 Volume 204, Issue 2, Page(s) 379–380

MeSH term(s)	Humans ; Monocytes ; Malaria ; Plasmodium falciparum
Language	English
Publishing date	2023-12-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.19259
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Article ; Online: Assessment of the neutralizing antibody response in Omicron breakthrough cases in healthcare workers who received the homologous booster of Moderna mRNA-1273.

Gillot, Constant / Tré-Hadry, Marie / Cupaiolo, Roberto / Blairon, Laurent / Wilmet, Alain / Beukinga, Ingrid / Dogné, Jean-Michel / Douxfils, Jonathan / Favresse, Julien

Virology

2024 Volume 595, Page(s) 110082

Abstract: Vaccines against SARS-CoV-2 were developed during the pandemic including the BNT162b2 and the mRNA-1273. We evaluated the levels of binding antibodies against the receptor binding domain and the levels of NAbs in individuals who developed a breakthrough ... ...

Abstract	Vaccines against SARS-CoV-2 were developed during the pandemic including the BNT162b2 and the mRNA-1273. We evaluated the levels of binding antibodies against the receptor binding domain and the levels of NAbs in individuals who developed a breakthrough infection after having received three doses of mRNA-1273. A total of 51 participants were included. The breakthrough group was compared to a 1:1 matched-control group. Among the 51 individuals, 18 (35%) developed a breakthrough infection. The GMT of NAbs against the BA.1 in the BK population was 278.1 (95%CI: 168.1-324.1). This titer was significantly lower compared to the matched-control group when considering all data (GMT = 477.4; 95%CI: 316.2-541.0; p = 0.0057). Results were similar for the BA.5 (GMT = 152.0 (95%CI: 76.9-172.9) for breakthrough and 262.0 (95%CI: 171.3-301.8) for control (p = 0.0043)). Our study found that individuals receiving the mRNA-1273 booster and who developed a breakthrough infection presented lower levels of binding antibodies and NAbs before the infection as compared to a matched-control group.
Language	English
Publishing date	2024-04-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	200425-2
ISSN	1096-0341 ; 0042-6822
ISSN (online)	1096-0341
ISSN	0042-6822
DOI	10.1016/j.virol.2024.110082
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Article ; Online: Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111:H21.

Tré-Hardy, Marie / De Rauw, Klara / Blairon, Laurent / Simon, Anne / Piérard, Denis

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

2023 Volume 131, Issue 5, Page(s) 206–216

Abstract: This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli (EAEC) O111:H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type ...

Abstract	This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli (EAEC) O111:H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type of outbreak. Clinical and microbiological investigations were needed to find a link between the cases and identify the causative agent. The microbiological procedure followed was first based on conventional analyses: isolation using selective cultures, identification by MALDI-TOF MS, antibiogram, determination of the serogroup by agglutination, then whole genome sequencing. A total of 7/21 children were infected with this pathogen. Four cases could be confirmed by a molecular technique, wgMLST, as belonging to the same bacterial clone. The action plan put in place focused on symptomatic case eviction, strict general hygiene precaution as well as specific cleaning and disinfection measures. The epidemic did last only a few days. It appears important, in the context of an epidemic, that clinical laboratories standardize their practice by equipping themselves with molecular techniques such as a multiplex which does not focus only on the serotype O157:H7 and which make it possible to distinguish the different pathotypes of E. coli by targeting several virulence genes (stx, aggR…). However, cost/effectiveness studies are awaited to confirm the interest of a systematic search by molecular method for the pathogen involved in a suspected outbreak occurring in a nursery.
MeSH term(s)	Child ; Humans ; Escherichia coli ; Serogroup ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Virulence/genetics ; Disease Outbreaks
Language	English
Publishing date	2023-03-03
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	93340-5
ISSN	1600-0463 ; 0903-4641
ISSN (online)	1600-0463
ISSN	0903-4641
DOI	10.1111/apm.13301
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Article ; Online: Clinical evaluation of the GSD NovaPrime® SARS-CoV-2 RTq-PCR assay.

Tré-Hardy, Marie / Piteüs, Sébastien / Beukinga, Ingrid / Blairon, Laurent

Diagnostic microbiology and infectious disease

2022 Volume 103, Issue 3, Page(s) 115718

Abstract: Faced with the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-throughput respiratory tests are in high demand. We evaluated the clinical performance of the GSD NovaPrime® SARS-CoV-2 RTq-PCR assay, a new assay that detects ...

Abstract	Faced with the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-throughput respiratory tests are in high demand. We evaluated the clinical performance of the GSD NovaPrime® SARS-CoV-2 RTq-PCR assay, a new assay that detects 2 specific RNA sequences of the nucleocapsid (N) gene. It was assessed using 99 nasopharyngeal samples and compared in parallel with the Allplex® assay. Among those samples, 72 and 27 were included in the positive (PPA) and negative (NPA) percent agreement analyses, respectively. In case of discordance, samples were reanalyzed with another amplification technique, the Aptima® SARS-CoV-2 assay. Cross-reactivity, including specimens positive for another respiratory virus and collected before the COVID-19 outbreak, was also evaluated (n = 32). Based on the patients' clinical history, the Ct (cycle threshold) values obtained, and the results of the Aptima® assay, the clinical performances were deemed satisfactory, with the PPA reaching a minimum percentage of 87.5% and the NPA reaching 100%. No cross-reactivity with other respiratory viruses was observed.
MeSH term(s)	COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Nasopharynx ; Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Sensitivity and Specificity
Language	English
Publishing date	2022-05-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2022.115718
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Article ; Online: Clinical evaluation of a dermatophyte RT-PCR assay and its impact on the turn-around-time: A prospective study.

Debuysschere, Cyril / Blairon, Laurent / Cupaiolo, Roberto / Beukinga, Ingrid / Tré-Hardy, Marie

Medical mycology

2023 Volume 61, Issue 8

Abstract: Onychomycosis is an important public health problem whose prevalence continues to grow and impact public health at several levels. Nevertheless, today the main diagnostic methods used in routine practice have many drawbacks. The aim of this study was to ... ...

Abstract	Onychomycosis is an important public health problem whose prevalence continues to grow and impact public health at several levels. Nevertheless, today the main diagnostic methods used in routine practice have many drawbacks. The aim of this study was to evaluate, for the first time, the clinical performance of a new multiplex polymerase chain reaction (PCR) (Novaplex®) in the identification of the causative agent on nail samples, and its impact on the turnaround time, compared to our traditional laboratory methods. From June 2022 to December 2022, all nail samples sent to our laboratory for suspected onychomycosis were included in this prospective study. We collected for each sample the results obtained with the Novaplex® PCR method and with the traditional direct microscopy examination and culture. Each discordant result was checked using a third method, which is another PCR method (DermaGenius® kit) as a resolver. For culture-positive samples, a turnaround time was calculated and compared to the one obtained with the Novaplex® method. A total of 131 samples were included. Among them, 5 were positive (3.8%) on direct microscopy, 33 were positive (25.2%) after culture, and 98 were negative (74.8%). All positive (n = 33) and negative (n = 69) cultures were also positive/negative with the Novaplex® PCR. Twenty-nine samples were positive with the Novaplex® method but negative with culture (discordant results). The percentage agreement between the culture and the Novaplex® methods was 77.9% (102 out of 131). While tested with the resolver (DermaGenius® PCR), 28 out of 29 discordant results were similarly found positive. The percentage agreement between the two PCR methods (Novaplex® and DermaGenius®) was 96.6%. The Novaplex® PCR method evaluated proved to be very reliable and allowed the direct identification of 62 out of 131 positive samples (47.3%) with the following distribution: 79.0% of Trichophyton rubrum complex, 11.3% of Trichophyton mentagrophytes complex, 6.5% of both Trichophyton rubrum complex and Trichophyton mentagrophytes complex, and 3.2% of Candida albicans. The median time [± 95% CI] for positive culture (between incubation and validation of the final identification) was 15 [12-23] days, while the turnaround time for the Novaplex® method adapted to our clinical laboratory routine is ≤7 days. Laboratory confirmation of onychomycosis is crucial and should always be obtained before starting treatment. The evaluated PCR method offered a rapid, reliable, robust, and inexpensive method of identification of the causative agent compared to traditional methods.
MeSH term(s)	Animals ; Onychomycosis/diagnosis ; Onychomycosis/veterinary ; Arthrodermataceae/genetics ; Prospective Studies ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; DNA, Fungal ; Sensitivity and Specificity ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/veterinary ; Trichophyton/genetics
Chemical Substances	DNA, Fungal
Language	English
Publishing date	2023-07-28
Publishing country	England
Document type	Journal Article
ZDB-ID	1421796-x
ISSN	1460-2709 ; 1369-3786
ISSN (online)	1460-2709
ISSN	1369-3786
DOI	10.1093/mmy/myad078
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Article: Enterococcus thailandicus

Mbouche, Patricia / Blairon, Laurent / Cupaiolo, Roberto / Zaouak, Yasmine / Hainaux, Bernard / Beukinga, Ingrid / Tré-Hardy, Marie

New microbes and new infections

2023 Volume 53, Page(s) 101137

Language	English
Publishing date	2023-04-23
Publishing country	England
Document type	Journal Article
ZDB-ID	2750179-6
ISSN	2052-2975
ISSN	2052-2975
DOI	10.1016/j.nmni.2023.101137
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Article ; Online: Atypical lymphocytes associated with monkeypox virus infection.

Debuysschere, Cyril / Beukinga, Ingrid / Hernando, Carmen / Blairon, Laurent / Tré-Hardy, Marie / Cupaiolo, Roberto

British journal of haematology

2022 Volume 199, Issue 3, Page(s) 306

MeSH term(s)	Humans ; Monkeypox virus/genetics ; Mpox (monkeypox) ; Lymphocytes
Language	English
Publishing date	2022-09-21
Publishing country	England
Document type	Journal Article
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.18480
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Article ; Online: Development and implementation of a RT-qPCR extraction-free protocol for the detection of SARS-CoV-2 and impact on the turn-around-time.

Blairon, Laurent / Piteüs, Sébastien / Beukinga, Ingrid / Tré-Hardy, Marie

Journal of medical virology

2021 Volume 93, Issue 4, Page(s) 2538–2542

Abstract: The occurrence of the COVID-19 second-wave outbreak in Europe has pushed laboratories performing molecular SARS-CoV-2 tests to increase their throughput and decrease the result rendering time. In this evaluation, we tested for the first time a new, ... ...

Abstract	The occurrence of the COVID-19 second-wave outbreak in Europe has pushed laboratories performing molecular SARS-CoV-2 tests to increase their throughput and decrease the result rendering time. In this evaluation, we tested for the first time a new, extraction-free, protocol with the Allplex SARS-CoV-2 Assay RT-qPCR kit on a Nimbus platform. Ninety-one samples, of which 71 previously tested positive with RT-qPCR with extraction were immediately analyzed without extraction, using only a dilution and thermal shock protocol. The positive and negative percentage agreements were respectively 97.2% (95% confidence interval [CI]: 0.90-0.99) and 95.0% (95% CI: 0.76-0.99). The two false negatives observed were very weakly positive with the comparison method. Moderate variations in Ct of the targeted genes were observed (median ± 95% CI): E gene, +2.49 ± 0.44; N gene, +0.98 ± 0.54; RdRP/S genes, +2.64 ± 0.48. On the other hand, the number of tests performed within 24 h raised from 86.4% to 97.8%, the turn-around time decreased from 19:18 to 09:03 (p < .0001), and the number of tests that can be performed per day doubled since this technique was introduced routinely in our laboratory.
MeSH term(s)	COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/methods ; Coronavirus Envelope Proteins/genetics ; Coronavirus Nucleocapsid Proteins/genetics ; Disease Outbreaks ; Europe ; Genes, Viral ; Humans ; Phosphoproteins/genetics ; RNA, Viral/analysis ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity
Chemical Substances	Coronavirus Envelope Proteins ; Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; RNA, Viral ; envelope protein, SARS-CoV-2 ; nucleocapsid phosphoprotein, SARS-CoV-2
Language	English
Publishing date	2021-01-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.26782
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Article ; Online: Waning antibodies in SARS-CoV-2 naïve vaccinees: Results of a three-month interim analysis of ongoing immunogenicity and efficacy surveillance of the mRNA-1273 vaccine in healthcare workers.

Tré-Hardy, Marie / Cupaiolo, Roberto / Wilmet, Alain / Beukinga, Ingrid / Blairon, Laurent

The Journal of infection

2021 Volume 83, Issue 3, Page(s) 381–412

MeSH term(s)	2019-nCoV Vaccine mRNA-1273 ; Antibodies, Viral ; COVID-19 ; COVID-19 Vaccines ; Health Personnel ; Humans ; Immunogenicity, Vaccine ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; COVID-19 Vaccines ; 2019-nCoV Vaccine mRNA-1273 (EPK39PL4R4)
Language	English
Publishing date	2021-06-20
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2021.06.017
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