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Article: Les vaccins à ARN anti-COVID-19.

Galibert, F

Bulletin de l'Academie nationale de medecine

2021 Volume 205, Issue 3, Page(s) 199–202

Title translation	Anti-COVID-19 RNA vaccines.
Language	French
Publishing date	2021-01-09
Publishing country	Netherlands
Document type	Editorial
ZDB-ID	213227-8
ISSN	0001-4079
ISSN	0001-4079
DOI	10.1016/j.banm.2021.01.001
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Article ; Online: Le séquençage de l’ADN, cinquante ans de prouesses technologiques et d’avancées scientifiques (1re partie).

Galibert, Francis

La Revue du praticien

2021 Volume 71, Issue 1, Page(s) 109–114

Title translation	DNA sequencing: fifty years of technological prowess and scientific advances (part 1).
MeSH term(s)	History, 19th Century ; History, 20th Century ; History, 21st Century ; Humans ; Sequence Analysis, DNA
Language	French
Publishing date	2021-06-23
Publishing country	France
Document type	Historical Article ; Journal Article
ZDB-ID	205365-2
ISSN	2101-017X ; 0035-2640
ISSN (online)	2101-017X
ISSN	0035-2640
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Article ; Online: Le séquençage de l’ADN, 50 ans de prouesses technologiques et d’avancées scientifiques (2e partie).

Galibert, Francis

La Revue du praticien

2021 Volume 71, Issue 2, Page(s) 225–230

Title translation	DNA sequencing: fifty years of technological prowess and scientific advances (part 2).
MeSH term(s)	History, 19th Century ; History, 20th Century ; History, 21st Century ; Humans ; Sequence Analysis, DNA
Language	French
Publishing date	2021-06-23
Publishing country	France
Document type	Historical Article ; Journal Article
ZDB-ID	205365-2
ISSN	2101-017X ; 0035-2640
ISSN (online)	2101-017X
ISSN	0035-2640
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Article ; Online: Baculovirus VP80 protein and the F-actin cytoskeleton interact and connect the viral replication factory with the nuclear periphery.

Marek, Martin / Merten, Otto-Wilhelm / Galibert, Lionel / Vlak, Just M / van Oers, Monique M

Journal of virology

2011 Volume 85, Issue 11, Page(s) 5350–5362

Abstract: ... protein is entirely localized in nuclei, adjacent to the virus-triggered F-actin scaffold that forms ...

Abstract	Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not able to migrate from the virogenic stroma to the nuclear periphery. In the current paper, we constructed a baculovirus recombinant with enhanced-green fluorescent protein (EGFP)-tagged VP80, allowing visualization of the VP80 distribution pattern during infection. In baculovirus-infected cells, the EGFP-VP80 protein is entirely localized in nuclei, adjacent to the virus-triggered F-actin scaffold that forms a highly organized three-dimensional network connecting the virogenic stroma physically with the nuclear envelope. Interaction between VP80 and host actin was confirmed by coimmunoprecipitation. We further showed that VP80 is associated with the nucleocapsid fraction of both BVs and ODVs, typically at one end of the nucleocapsids. In addition, the presence of sequence motifs with homology to invertebrate paramyosin proteins strongly supports a role for VP80 in the polar transport of nucleocapsids to the periphery of the nucleus on their way to the plasma membrane to form BVs and for assembly in the nuclear periphery to form ODVs for embedding in viral occlusion bodies.
MeSH term(s)	Actins/metabolism ; Animals ; Artificial Gene Fusion ; Capsid Proteins/metabolism ; Cell Line ; Cell Nucleus/chemistry ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Host-Pathogen Interactions ; Immunoprecipitation ; Lepidoptera ; Nucleopolyhedroviruses/pathogenicity ; Protein Binding ; Protein Interaction Mapping ; Staining and Labeling/methods
Chemical Substances	Actins ; Capsid Proteins ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9) ; Ac-vp80 protein, Autographa californica nucleopolyhedrovirus (148686-07-1)
Language	English
Publishing date	2011-03-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.00035-11
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Article ; Online: Are the Olfactory Receptors Present at the Sperm Membrane Involved in Reproduction?

Galibert, Francis / Azzouzi, Naoual

International journal of molecular sciences

2023 Volume 24, Issue 14

Abstract: Olfactory receptors (ORs), key components in ensuring the detection of myriad odorants, are expressed not only on the surface of olfactory neurons but also in many other tissues. In the case of ORs expressed at the sperm membrane, in vitro experiments ... ...

Abstract	Olfactory receptors (ORs), key components in ensuring the detection of myriad odorants, are expressed not only on the surface of olfactory neurons but also in many other tissues. In the case of ORs expressed at the sperm membrane, in vitro experiments with human and mouse spermatozoids have shown that they move toward the regions with the highest concentration of bourgeonal and lyral, respectively. However, to date, no in vivo experiment has shown any biological function of these ORs. To demonstrate a possible role in vivo of ORs in sperm chemotaxis, we overloaded the vaginal space of female mice from the prolific Swiss CD1 strain with lyral to induce competition with the supposed natural ligand and to prevent its detection. As shown, the mice that received lyral had much fewer newborns than the control mice treated with PBS, showing that lyral has a strong negative impact on procreation. This indicates that the ORs at the sperm surface are biologically active and make an important contribution to reproduction. Control experiments performed with hexanal, which does not alter sperm movement in vitro, indicate that the inhibition of reproduction observed was specific to lyral. In addition, we show that males are attracted to the smell of lyral, which acts as a pheromone, and prefer to copulate with mice marked on their back with lyral rather than with those that have not been marked. These results suggest an explanation for some cases of human infertility, which could result from an absence of recognition between the natural ligand and the ORs, either due to a mutation or a lack of expression from one of the two partners, allowing for the development of a diagnostic tests. These results might also lead to the development of a novel contraception strategy based on the use of vaginal tablets delivering an odorant or a drug that competes with the natural ligand.
MeSH term(s)	Infant, Newborn ; Humans ; Male ; Mice ; Female ; Animals ; Receptors, Odorant/metabolism ; Ligands ; Semen/metabolism ; Spermatozoa/metabolism ; Odorants ; Reproduction ; Olfactory Receptor Neurons/metabolism
Chemical Substances	hydroxyisohexyl 3-cyclohexene carboxaldehyde (QUE43B9Z2Q) ; Receptors, Odorant ; Ligands
Language	English
Publishing date	2023-07-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241411277
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Article ; Online: Baculovirus VP80 Protein and the F-Actin Cytoskeleton Interact and Connect the Viral Replication Factory with the Nuclear Periphery

Marek, M. / Merten, O.W. / Galibert, L. / Vlak, J.M. / van Oers, M.M.

Journal of Virology

2011 Volume 85, Issue 11

Abstract: ... protein is entirely localized in nuclei, adjacent to the virus-triggered F-actin scaffold that forms ...

Abstract	Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not able to migrate from the virogenic stroma to the nuclear periphery. In the current paper, we constructed a baculovirus recombinant with enhanced-green fluorescent protein (EGFP)-tagged VP80, allowing visualization of the VP80 distribution pattern during infection. In baculovirus-infected cells, the EGFP-VP80 protein is entirely localized in nuclei, adjacent to the virus-triggered F-actin scaffold that forms a highly organized three-dimensional network connecting the virogenic stroma physically with the nuclear envelope. Interaction between VP80 and host actin was confirmed by coimmunoprecipitation. We further showed that VP80 is associated with the nucleocapsid fraction of both BVs and ODVs, typically at one end of the nucleocapsids. In addition, the presence of sequence motifs with homology to invertebrate paramyosin proteins strongly supports a role for VP80 in the polar transport of nucleocapsids to the periphery of the nucleus on their way to the plasma membrane to form BVs and for assembly in the nuclear periphery to form ODVs for embedding in viral occlusion bodies
Keywords	autographa-californica nucleopolyhedrovirus ; dna-replication ; efficient egress ; mammalian-cells ; multicapsid nucleopolyhedrovirus ; multiple sequence alignment ; new-generation ; occlusion-derived virus ; orgyia-pseudotsugata ; polyhedrosis-virus
Subject code	570
Language	English
Publishing country	nl
Document type	Article ; Online
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Systematic sequencing of the human HLA-A/HLA-F region: establishment of a cosmid contig and identification of a new gene cluster within 37 kb of sequence.

Lepourcelet, M / Andrieux, N / Giffon, T / Pichon, L / Hampe, A / Galibert, F / Mosser, J

Genomics

1996 Volume 37, Issue 3, Page(s) 316–326

Abstract: ... of the HLA-A/HLA-F region with a view to defining its contents in genes and pseudogenes ...

Abstract	The class I region of the human histocompatibility complex is characterized by a high density of genes and pseudogenes and a complex structural organization. To elucidate the complete structure of the HLA-A/HLA-F region with a view to defining its contents in genes and pseudogenes, we developed a strategy of systematic sequencing. This report describes the establishment of a cosmid contig spanning most of the region and the analysis of a 37-kb sequence from one of the cosmids. Four new genes, organized with the HCG-V gene in a clustered structure, have been identified. Two of these contain a zinc finger motif characteristic of DNA-binding proteins. The former, a member of the C3HC4 protein family, is highly expressed in prostate and contains a B30-2-like sequence identified in several genes mapped within the class I region. The latter, which is ubiquitously expressed, is the human equivalent of the yeast polymerase IA12.2 subunit and of the murine tctex6 gene. Of the two other genes, one remains an anonymous gene with no particular feature, while the fourth, specifically expressed in testis, is the human equivalent of the murine tctex4 gene. This cluster, located in a region corresponding to a syntenic unit between mouse and human, appears to be highly conserved.
MeSH term(s)	Amino Acid Sequence ; Animals ; Base Sequence ; Chromosome Walking ; Chromosomes, Human, Pair 6/genetics ; Cosmids/genetics ; DNA-Binding Proteins/genetics ; Dyneins ; Evolution, Molecular ; Genes ; Genes, MHC Class I ; HLA Antigens/genetics ; HLA-A Antigens/genetics ; Hemochromatosis/genetics ; Histocompatibility Antigens Class I/genetics ; Humans ; Intracellular Signaling Peptides and Proteins ; Male ; Mice ; Microtubule-Associated Proteins ; Molecular Sequence Data ; Nuclear Proteins/genetics ; Sequence Alignment ; Sequence Homology ; Species Specificity ; Testis/metabolism ; Zinc Fingers/genetics ; t-Complex Genome Region
Chemical Substances	DNA-Binding Proteins ; HLA Antigens ; HLA-A Antigens ; HLA-F antigens ; Histocompatibility Antigens Class I ; Intracellular Signaling Peptides and Proteins ; Microtubule-Associated Proteins ; Nuclear Proteins ; PPP1R11 protein, human ; Tcte3 protein, mouse ; Dyneins (EC 3.6.4.2)
Language	English
Publishing date	1996-11-01
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	356334-0
ISSN	1089-8646 ; 0888-7543
ISSN (online)	1089-8646
ISSN	0888-7543
DOI	10.1006/geno.1996.0566
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Article: Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome.

Galibert, F / Hérissé, J / Courtois, G

Gene

1979 Volume 6, Issue 1, Page(s) 1–22

Abstract: ... 2 EcoRI-F fragment was determined. Information contained in that nucleotide sequence, which is 1743 ... by the EcoRI-F fragment. A method to rapidly determine the cleavage site of restriction endonucleases is also ...

Abstract	Using the DNA sequence method of Maxam and Gilbert the entire nucleotide sequence of the adenovirus 2 EcoRI-F fragment was determined. Information contained in that nucleotide sequence, which is 1743 base pairs long, is interpreted with respect to the mapping and processing of the three mRNAs partly encoded by the EcoRI-F fragment. A method to rapidly determine the cleavage site of restriction endonucleases is also reported.
MeSH term(s)	Adenoviridae/genetics ; Base Sequence ; Chromosome Mapping ; DNA Restriction Enzymes/metabolism ; DNA, Viral/analysis ; Electrophoresis, Polyacrylamide Gel ; Genes ; RNA, Messenger/genetics
Chemical Substances	DNA, Viral ; RNA, Messenger ; DNA Restriction Enzymes (EC 3.1.21.-)
Language	English
Publishing date	1979-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/0378-1119(79)90081-7
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Article ; Online: Dog olfactory receptor gene expression profiling using samples derived from nasal epithelium brushing.

Azzouzi, Naoual / Guillory, Anne-Sophie / Chaudieu, Gilles / Galibert, Francis

Canine medicine and genetics

2022 Volume 9, Issue 1, Page(s) 7

Abstract: Dogs have an exquisite sense of olfaction. In many instances this ability has been utilized by humans for a wide range of important situations including detecting explosives and illegal drugs. It is accepted that some breeds have better senses of smell ... ...

Abstract	Dogs have an exquisite sense of olfaction. In many instances this ability has been utilized by humans for a wide range of important situations including detecting explosives and illegal drugs. It is accepted that some breeds have better senses of smell than others. Dogs can detect many volatile compounds at extremely low concentrations in air. To achieve such high levels of detection, the canine olfactory system is both complex and highly developed requiring a high density of olfactory receptors capable of detecting volatiles. Consequently the dog genome encodes a large number of olfactory receptor (OR) genes. However, it remains unclear as to what extent are all of these OR genes expressed on the cell surface. To facilitate such studies, a nasal brushing method was developed to recover dog nasal epithelial cell samples from which total RNA could be extracted and used to prepare high quality cDNA libraries. After capture by hybridization with an extensive set of oligonucleotides, the level of expression of each transcript was measured following next generation sequencing (NGS). The reproducibility of this sampling approach was checked by analyzing replicate samples from the same animal (up to 6 per each naris). The quality of the hybridization capture was also checked by analyzing two DNA libraries; this offered an advantage over RNA libraries by having an equal presence for each gene. Finally, we compared this brushing method performed on living dogs to a nasal epithelium biopsy approach applied to two euthanized terminally ill dogs, following consent from their owners.Comparison the expression levels of each transcript indicate that the ratios of expression between the highest and the least expressed OR in each sample are greater than 10,000 (paralog variation). Furthermore, it was clear that a number of OR genes are not expressed.The method developed and described here will allow researchers to further address whether variations observed in the OR transcriptome relate to dog 'life experiences' and whether any differences observed between samples are dog-specific or breed-specific.
Language	English
Publishing date	2022-05-20
Publishing country	England
Document type	Journal Article
ISSN	2662-9380
ISSN (online)	2662-9380
DOI	10.1186/s40575-022-00116-7
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Article: Mapping the DNA fragments produced by cleavage of EcoRI F fragment of adenovirus 2 with HaeIII, HpaII and AluI.

Hérissé, J / Courtois, G / Galibert, F

Gene

1978 Volume 4, Issue 4, Page(s) 279–294

Abstract: Hydrolysis of the EcoRI F fragment of the adenovirus 2 genome with HaeIII, HpaII and AluI ... other enzymes. Position of the KpnI, HindIII, MboI and SmaI sites within the EcoRI F fragment were also ...

Abstract	Hydrolysis of the EcoRI F fragment of the adenovirus 2 genome with HaeIII, HpaII and AluI restriction enzymes gives respectively 9, 11, and 11 fragments, the size of which ranges from 20 bp for the smallest HpaII k fragment to 585 for the largest AluI A fragment. The order of fragments was mainly deduced from partial hydrolysis analyses. The relative order of all restriction sites and the distance in nucleotides between them were obtained through secondary analyses of each restriction fragment by the two other enzymes. Position of the KpnI, HindIII, MboI and SmaI sites within the EcoRI F fragment were also reevaluated.
MeSH term(s)	Adenoviridae/enzymology ; Adenoviridae/genetics ; Base Sequence ; Chromosome Mapping ; DNA Restriction Enzymes/genetics ; DNA, Viral/genetics ; Electrophoresis, Polyacrylamide Gel ; Genes, Viral ; Hydrolysis ; Transcription Factors ; Transcription, Genetic
Chemical Substances	DNA, Viral ; Transcription Factors ; DNA Restriction Enzymes (EC 3.1.21.-)
Language	English
Publishing date	1978-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/0378-1119(78)90046-x
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