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Book ; Online: Metodología de la investigación, ¿para qué?

Cohen, Néstor / Gómez Rojas, Gabriela / Riveiro, Manuel

La producción de los datos y los diseños

2019

Keywords	Social research & statistics ; Sociology
Language	Spanish
Size	1 Online-Ressource
Publisher	CLACSO
Document type	Book ; Online
Note	Spanish
HBZ-ID	HT030611421
ISBN	9789877231908 ; 9877231905
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Photosynthetic light harvesting and energy conversion.

Fleming, Graham R / Minagawa, Jun / Renger, Thomas / Schlau-Cohen, Gabriela S

The Journal of chemical physics

2023 Volume 159, Issue 10

Language	English
Publishing date	2023-09-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	3113-6
ISSN	1089-7690 ; 0021-9606
ISSN (online)	1089-7690
ISSN	0021-9606
DOI	10.1063/5.0170807
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Article ; Online: The second empty nest: The lived experience of older women whose intensive 'grandmotherhood' has ended.

Cohen, Yarin / Spector-Mersel, Gabriela / Shiovitz-Ezra, Sharon

Journal of aging studies

2023 Volume 66, Page(s) 101163

Abstract: Grandmothers are the major nonparental unpaid source of childcare in Western societies. Intensive caring for grandchildren may pose challenges to some grandmothers, but also offers an opportunity to refill the 'empty nest' often experienced in mid-life. ... ...

Abstract	Grandmothers are the major nonparental unpaid source of childcare in Western societies. Intensive caring for grandchildren may pose challenges to some grandmothers, but also offers an opportunity to refill the 'empty nest' often experienced in mid-life. When grandmothers' intensive involvement in their grandchildren's care decreases significantly or ceases altogether, they may experience a recurrence of the empty nest syndrome. This may be particularly powerful in the familial and pro-natalist Israeli society, where caring for children is a central tenet of femininity. Despite the growing numbers of grandmothers whose intensive involvement in caring for their grandchildren has ended, this transition has been overlooked socially and rarely examined empirically. To fill this void, the present study examined the lived experience of these grandmothers and the relevance of the concept of the 'second empty nest' in this context. Within a phenomenological study, in-depth interviews were conducted with 11 Israeli women whose intensive 'grandmotherhood' (childcare occurring at least three times per week, for at least two hours each day, for a minimum of two years) has ended. These interviews were analyzed according to Moustakas' phenomenological analysis. The analysis revealed four themes: the circumstances of the cessation of intensive childcare involvement; difficulties and challenges experienced; positive aspects associated with it; and behavioral and cognitive strategies utilized to cope with the void in grandmothers' lives. The grandmothers' experiences reveal a significant similarity to that reported by mothers undergoing the empty nest syndrome. Hence, we offer the term 'the second empty nest' to represent the phenomenon of grandmothers' cessation of intensive childcare. Alongside the similarities between the two empty nests, the challenges of the second transition seem more intense than those posed by the first. This is due to the different locations of mothers and grandmothers across the lifespan and the intersection between sexism and ageism that underlies Western societies. Possible practices to assist grandmothers undergoing the second empty nest are suggested.
MeSH term(s)	Humans ; Female ; Aged ; Ageism ; Femininity ; Longevity ; Mothers ; Sexism
Language	English
Publishing date	2023-08-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2006012-9
ISSN	1879-193X ; 0890-4065
ISSN (online)	1879-193X
ISSN	0890-4065
DOI	10.1016/j.jaging.2023.101163
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Article ; Online: DAPT Duration: When Less Is More.

Cohen, Marc / Andries, Gabriela

Cardiovascular revascularization medicine : including molecular interventions

2020 Volume 21, Issue 12, Page(s) 1507–1508

MeSH term(s)	Drug-Eluting Stents ; Dual Anti-Platelet Therapy ; Humans ; Percutaneous Coronary Intervention/adverse effects ; Platelet Aggregation Inhibitors/adverse effects
Chemical Substances	Platelet Aggregation Inhibitors
Language	English
Publishing date	2020-09-16
Publishing country	United States
Document type	Editorial ; Comment
ZDB-ID	2212113-4
ISSN	1878-0938 ; 1553-8389
ISSN (online)	1878-0938
ISSN	1553-8389
DOI	10.1016/j.carrev.2020.09.018
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Article ; Online: Energetic robustness to large scale structural fluctuations in a photosynthetic supercomplex.

Harris, Dvir / Toporik, Hila / Schlau-Cohen, Gabriela S / Mazor, Yuval

Nature communications

2023 Volume 14, Issue 1, Page(s) 4650

Abstract: Photosynthetic organisms transport and convert solar energy with near-unity quantum efficiency using large protein supercomplexes held in flexible membranes. The individual proteins position chlorophylls to tight tolerances considered critical for fast ... ...

Abstract	Photosynthetic organisms transport and convert solar energy with near-unity quantum efficiency using large protein supercomplexes held in flexible membranes. The individual proteins position chlorophylls to tight tolerances considered critical for fast and efficient energy transfer. The variability in protein organization within the supercomplexes, and how efficiency is maintained despite variability, had been unresolved. Here, we report on structural heterogeneity in the 2-MDa cyanobacterial PSI-IsiA photosynthetic supercomplex observed using Cryo-EM, revealing large-scale variances in the positions of IsiA relative to PSI. Single-molecule measurements found efficient IsiA-to-PSI energy transfer across all conformations, along with signatures of transiently decoupled IsiA. Structure based calculations showed that rapid IsiA-to-PSI energy transfer is always maintained, and even increases by three-fold in rare conformations via IsiA-specific chls. We postulate that antennae design mitigates structural fluctuations, providing a mechanism for robust energy transfer in the flexible membrane.
MeSH term(s)	Photosystem I Protein Complex/metabolism ; Light-Harvesting Protein Complexes/metabolism ; Bacterial Proteins/metabolism ; Photosynthesis ; Cyanobacteria/metabolism
Chemical Substances	Photosystem I Protein Complex ; Light-Harvesting Protein Complexes ; Bacterial Proteins
Language	English
Publishing date	2023-08-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-40146-8
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Article ; Online: Photoprotective conformational dynamics of photosynthetic light-harvesting proteins.

Manna, Premashis / Schlau-Cohen, Gabriela S

Biochimica et biophysica acta. Bioenergetics

2022 Volume 1863, Issue 4, Page(s) 148543

Abstract: Under high light conditions, excess energy can damage the machinery of oxygenic photosynthesis. Plants have evolved a series of photoprotective processes, including conformational changes of the light-harvesting complexes that activate dissipation of ... ...

Abstract	Under high light conditions, excess energy can damage the machinery of oxygenic photosynthesis. Plants have evolved a series of photoprotective processes, including conformational changes of the light-harvesting complexes that activate dissipation of energy as heat. In this mini-review, we will summarize our recent work developing and applying single-molecule methods to investigate the conformational states of the light-harvesting complexes. Through these measurements, we identified dissipative conformations and how they depend on conditions that mimic high light. Our studies revealed an equilibrium between the light-harvesting and dissipative conformations, and that the nature of the equilibrium varies with cellular environment, between proteins, and between species. Finally, we conclude with an outlook on open questions and implications for photosynthetic yields.
MeSH term(s)	Light-Harvesting Protein Complexes/metabolism ; Photosynthesis/physiology ; Protein Conformation ; Tumor Necrosis Factor Ligand Superfamily Member 14/metabolism
Chemical Substances	Light-Harvesting Protein Complexes ; Tumor Necrosis Factor Ligand Superfamily Member 14
Language	English
Publishing date	2022-02-21
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	60-7
ISSN	1879-2650 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2650 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbabio.2022.148543
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Article ; Online: Engineering Exciton Dynamics with Synthetic DNA Scaffolds.

Hart, Stephanie M / Gorman, Jeffrey / Bathe, Mark / Schlau-Cohen, Gabriela S

Accounts of chemical research

2023 Volume 56, Issue 15, Page(s) 2051–2061

Abstract: Excitons are the molecular-scale currency of electronic energy. Control over excitons enables energy to be directed and harnessed for light harvesting, electronics, and sensing. Excitonic circuits achieve such control by arranging electronically active ... ...

Abstract	Excitons are the molecular-scale currency of electronic energy. Control over excitons enables energy to be directed and harnessed for light harvesting, electronics, and sensing. Excitonic circuits achieve such control by arranging electronically active molecules to prescribe desired spatiotemporal dynamics. Photosynthetic solar energy conversion is a canonical example of the power of excitonic circuits, where chromophores are positioned in a protein scaffold to perform efficient light capture, energy transport, and charge separation. Synthetic systems that aim to emulate this functionality include self-assembled aggregates, molecular crystals, and chromophore-modified proteins. While the potential of this approach is clear, these systems lack the structural precision to control excitons or even test the limits of their power. In recent years, DNA origami has emerged as a designer material that exploits biological building blocks to construct nanoscale architectures. The structural precision afforded by DNA origami has enabled the pursuit of naturally inspired organizational principles in a highly precise and scalable manner. In this Account, we describe recent developments in DNA-based platforms that spatially organize chromophores to construct tunable excitonic systems. The high fidelity of DNA base pairing enables the formation of programmable nanoscale architectures, and sequence-specific placement allows for the precise positioning of chromophores within the DNA structure. The integration of a wide range of chromophores across the visible spectrum introduces spectral tunability. These excitonic DNA-chromophore assemblies not only serve as model systems for light harvesting, solar conversion, and sensing but also lay the groundwork for the integration of coupled chromophores into larger-scale nucleic acid architectures.We have used this approach to generate DNA-chromophore assemblies of strongly coupled delocalized excited states through both sequence-specific self-assembly and the covalent attachment of chromophores. These strategies have been leveraged to independently control excitonic coupling and system-bath interaction, which together control energy transfer. We then extended this framework to identify how scaffold configurations can steer the formation of symmetry-breaking charge transfer states, paving the way toward the design of dual light-harvesting and charge separation DNA machinery. In an orthogonal application, we used the programmability of DNA chromophore assemblies to change the optical emission properties of strongly coupled dimers, generating a series of fluorophore-modified constructs with separable emission properties for fluorescence assays. Upcoming advances in the chemical modification of nucleotides, design of large-scale DNA origami, and predictive computational methods will aid in constructing excitonic assemblies for optical and computing applications. Collectively, the development of DNA-chromophore assemblies as a platform for excitonic circuitry offers a pathway to identifying and applying design principles for light harvesting and molecular electronics.
MeSH term(s)	Photosynthesis ; Energy Transfer ; Fluorescent Dyes ; DNA/chemistry
Chemical Substances	Fluorescent Dyes ; DNA (9007-49-2)
Language	English
Publishing date	2023-06-22
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1483291-4
ISSN	1520-4898 ; 0001-4842
ISSN (online)	1520-4898
ISSN	0001-4842
DOI	10.1021/acs.accounts.3c00086
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Article ; Online: Robust Prediction and Protein Selection with Adaptive PENSE.

Kepplinger, David / Cohen Freue, Gabriela V

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2426, Page(s) 315–331

Abstract: Adaptive PENSE is a method that can be used to build models for predicting clinical outcomes from a small subset of a potentially large number of candidate proteins. Adaptive PENSE is designed to give reliable results under two common challenges often ... ...

Abstract	Adaptive PENSE is a method that can be used to build models for predicting clinical outcomes from a small subset of a potentially large number of candidate proteins. Adaptive PENSE is designed to give reliable results under two common challenges often encountered in these kinds of studies: (1) the number of samples with known clinical outcome and proteomic data is small, while the number of candidate proteins is large and/or (2) proteomic data and the clinical outcome measurements suffer from data quality issues in a small fraction of samples. Even in the presence of these challenges, adaptive PENSE reliably identifies proteins relevant for prediction and estimates accurate predictive models. Adaptive PENSE is designed to be resilient to data quality issues in up to 50% of samples. Almost half of the samples could have aberrant values in the measured protein levels and clinical outcome values without causing severe detrimental effects to the estimated predictive model. The method is implemented as an R package and supports the user in the model selection process by automating most steps and providing diagnostic visualizations to guide the user. Users can choose among several predictive models to select the model with high prediction accuracy and an appropriate number of selected proteins.
MeSH term(s)	Proteomics ; Proteins/genetics ; Research Design
Chemical Substances	Proteins
Language	English
Publishing date	2022-10-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-1967-4_14
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Article ; Online: Ultrafast Dynamics of Photosynthetic Light Harvesting: Strategies for Acclimation Across Organisms.

Fiebig, Olivia C / Harris, Dvir / Wang, Dihao / Hoffmann, Madeline P / Schlau-Cohen, Gabriela S

Annual review of physical chemistry

2023 Volume 74, Page(s) 493–520

Abstract: Photosynthetic light harvesting exhibits near-unity quantum efficiency. The high efficiency is achieved through a series of energy and charge transfer steps within a network of pigment-containing proteins. Remarkably, high efficiency is conserved across ... ...

Abstract	Photosynthetic light harvesting exhibits near-unity quantum efficiency. The high efficiency is achieved through a series of energy and charge transfer steps within a network of pigment-containing proteins. Remarkably, high efficiency is conserved across many organisms despite differences in the protein structures and organization that allow each organism to respond to its own biological niche and the stressors within. In this review, we highlight recent progress toward understanding how organisms maintain optimal light-harvesting ability by acclimating to their environment. First, we review the building blocks of photosynthetic light harvesting, energy transfer, and time-resolved spectroscopic techniques. Then, we explore how three classes of photosynthetic organisms-purple bacteria, cyanobacteria, and green plants-optimize their light-harvesting apparatuses to their particular environment. Overall, research has shown that photosynthetic energy transfer is robust to changing environmental conditions, with each organism utilizing its own strategies to optimize photon capture in its particular biological niche.
MeSH term(s)	Light-Harvesting Protein Complexes/chemistry ; Light-Harvesting Protein Complexes/metabolism ; Photosynthesis ; Spectrum Analysis ; Acclimatization ; Light
Chemical Substances	Light-Harvesting Protein Complexes
Language	English
Publishing date	2023-02-15
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	1470474-2
ISSN	1545-1593 ; 0066-426X
ISSN (online)	1545-1593
ISSN	0066-426X
DOI	10.1146/annurev-physchem-083122-111318
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Article ; Online: Birth outcomes associated with a natural delivery approach in a perinatal center: A comparative retrospective study.

Abu Shqara, Raneen / Goldinfeld, Gabriela / Tenne Cohen, Roni / Nahir Biderman, Sari / Lowenstein, Lior / Frank Wolf, Maya

The journal of obstetrics and gynaecology research

2024

Abstract: Objectives: At our center, natural home-like delivery settings have been established in or near conventional labor wards, for the care of pregnant women who prefer little or no medical intervention during labor and birth. We compared obstetrical and ... ...

Abstract	Objectives: At our center, natural home-like delivery settings have been established in or near conventional labor wards, for the care of pregnant women who prefer little or no medical intervention during labor and birth. We compared obstetrical and neonatal outcomes of women in active spontaneous labor, between those who chose to deliver in a natural-delivery setup and those who chose a conventional setting. Methods: This retrospective study included low-risk women who delivered at term between March 1, 2020 and December 31, 2022, in a single tertiary university affiliated medical center. Birth outcomes were compared between 124 women who delivered by natural birth (the study group) and 244 who gave birth in a conventional setting (the control group). Results: No cesarean deliveries were performed in the study group, compared to 18 (7.4%) of the control group, p = 0.004. Intrapartum fever, postpartum hemorrhage, and uterotonic administration were similar between the groups. For the study compared to the control group, breastfeeding was more common (71.3% vs. 12.3%, p < 0.001), analgesia administration within 48 h delivery was lower (4.1% vs. 10.7%, p = 0.033), and maternal and neonatal length of hospitalization were shorter. Of the women initially admitted to the natural-delivery room, 14 (11.5%) were transferred to a conventional-delivery room. Conclusions: Birth in a hospital natural-delivery setting was associated with increased likelihood of vaginal birth, increased immediate breastfeeding and breastfeeding at discharge, and lower postpartum pain.
Language	English
Publishing date	2024-04-15
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1327307-3
ISSN	1447-0756 ; 1341-8076
ISSN (online)	1447-0756
ISSN	1341-8076
DOI	10.1111/jog.15952
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