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Article ; Online: Ligilactobacillus acidipiscis

Shen, Fei / Wang, Qianqian / Ullah, Sami / Pan, Ya / Zhao, Minjie / Wang, Jing / Chen, Ming / Feng, Fengqin / Zhong, Hao

Food & function

2024 Volume 15, Issue 1, Page(s) 310–325

Abstract: Constipation is a prevalent gastrointestinal (GI) problem affecting a large number of individuals. This study aimed to investigate peristalsis-promoting potential characteristics ... ...

Abstract	Constipation is a prevalent gastrointestinal (GI) problem affecting a large number of individuals. This study aimed to investigate peristalsis-promoting potential characteristics of
MeSH term(s)	Mice ; Animals ; Zebrafish ; Gastrointestinal Microbiome ; RNA, Ribosomal, 16S/genetics ; Constipation/drug therapy ; Constipation/chemically induced ; Water/pharmacology
Chemical Substances	RNA, Ribosomal, 16S ; Water (059QF0KO0R)
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2612033-1
ISSN	2042-650X ; 2042-6496
ISSN (online)	2042-650X
ISSN	2042-6496
DOI	10.1039/d3fo03259k
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Article ; Online: Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9.

Zhu, Yuguang / Hao, Fengqin

International journal of molecular medicine

2021 Volume 47, Issue 5

Abstract: A number of studies have verified the vital effects of long non‑coding RNAs on the malignant behaviour of retinoblastoma (RB). The objective of the present study was to examine the specific role and mechanisms of HLA complex P5 (HCP5) in RB. For this ... ...

Abstract	A number of studies have verified the vital effects of long non‑coding RNAs on the malignant behaviour of retinoblastoma (RB). The objective of the present study was to examine the specific role and mechanisms of HLA complex P5 (HCP5) in RB. For this purpose, reverse transcription‑quantitative polymerase chain reaction was used to determine the expression of HCP5, microRNA (miRNA/miR)‑3619‑5p and histone deacetylase 9 (HDAC9). A 3‑(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2‑H-tetrazolium bromide assay was conducted to detect cell viability. Transwell assays were used to evaluate the abilities of cell migration and invasion. A mouse tumor model was established to explore the functions of HCP5 in RB
MeSH term(s)	Animals ; Cell Line, Tumor ; Cell Movement/genetics ; Female ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Histone Deacetylases/genetics ; Humans ; Mice, Inbred BALB C ; MicroRNAs/genetics ; RNA, Long Noncoding/genetics ; Repressor Proteins/genetics ; Retinal Neoplasms/genetics ; Retinal Neoplasms/pathology ; Retinoblastoma/genetics ; Retinoblastoma/pathology ; Xenograft Model Antitumor Assays ; Mice
Chemical Substances	HCP5 long noncoding RNA, human ; MIRN-3619 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; Repressor Proteins ; HDAC9 protein, human (EC 3.5.1.98) ; Histone Deacetylases (EC 3.5.1.98)
Language	English
Publishing date	2021-03-11
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1444428-8
ISSN	1791-244X ; 1107-3756
ISSN (online)	1791-244X
ISSN	1107-3756
DOI	10.3892/ijmm.2021.4907
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Article: Lactobacillus plantarum

Zhong, Hao / Wang, Jian / Abdullah / Hafeez, Muhammad Adnan / Guan, Rongfa / Feng, Fengqin

Frontiers in nutrition

2021 Volume 8, Page(s) 754222

Abstract: Lactobacillus ... ...

Abstract	Lactobacillus plantarum
Language	English
Publishing date	2021-10-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2776676-7
ISSN	2296-861X
ISSN	2296-861X
DOI	10.3389/fnut.2021.754222
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Article ; Online: A trivalent aptasensor by using DNA tetrahedron as scaffold for label-free determination of antibiotics.

Ye, Tai / Xu, Yimin / Chen, Haohao / Yuan, Min / Cao, Hui / Hao, Liling / Wu, Xiuxiu / Yin, Fengqin / Xu, Fei

Biosensors & bioelectronics

2024 Volume 251, Page(s) 116127

Abstract: Owing to advantage in high sensitivity and fast response, aptamer based electrochemical biosensors have attracted much more attention. However, inappropriate interfacial engineering strategy leads to poor recognition performance, which ascribe to the ... ...

Abstract	Owing to advantage in high sensitivity and fast response, aptamer based electrochemical biosensors have attracted much more attention. However, inappropriate interfacial engineering strategy leads to poor recognition performance, which ascribe to the following factors of immobilized oligonucleotide strand including steric hindrance, interchain entanglement, and unfavorable conformation. In this work, we proposed a DNA tetrahedron based diblock aptamer immobilized strategy for the construction of label-free electrochemical biosensor. The diblock aptamer sequence is composite of T-rich anchor domain and recognition domain, where T-rich domain enabling anchored on the edge of DNA tetrahedron via Hoogsteen hydrogen bond at neutral condition. The DNA tetrahedron scaffold offers an appropriate lateral space for target recognition of diblock aptamer. More importantly, this trivalent aptamer recognition interface can be regenerated by simply adjusting the pH environment to alkaline, resulting in the dissociation of diblock aptamer. Under the optimum condition, proposed electrochemical aptasensor manifested a satisfied sensitivity for aminoglycosides antibiotic, kanamycin with a limit of detection of 0.69 nM, which is 45-fold lower than traditional Au-S immobilization strategy. Moreover, the proposed aptasensor had also successfully been extended to ampicillin detection by changing the sequence of recognition domain in diblock aptamer. This work paves a new way for the rational design of aptamer-based electrochemical sensor.
MeSH term(s)	Anti-Bacterial Agents ; Biosensing Techniques/methods ; Aptamers, Nucleotide/chemistry ; DNA/chemistry ; Kanamycin ; Electrochemical Techniques ; Limit of Detection ; Gold/chemistry
Chemical Substances	Anti-Bacterial Agents ; Aptamers, Nucleotide ; DNA (9007-49-2) ; Kanamycin (59-01-8) ; Gold (7440-57-5)
Language	English
Publishing date	2024-02-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2024.116127
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Article ; Online: A smartphone sensing platform for the sensitive and selective detection of clothianidin based on MIP-functionalized lanthanide MOF.

Cao, Hui / Han, Yiyi / Chen, Zixin / Ding, Xiner / Ye, Tai / Yuan, Min / Yu, Jinsong / Wu, Xiuxiu / Hao, Liling / Yin, Fengqin / Xu, Fei

Mikrochimica acta

2024 Volume 191, Issue 4, Page(s) 172

Abstract: A novel molecularly imprinted nanomaterial (Eu (BTC)-MPS@MIP) was synthesized on the surface of silanized europium-based metal-organic frameworks (Eu (BTC)-MPS) using 1, 3, 5-benzotrioic acid ( ... ...

Abstract	A novel molecularly imprinted nanomaterial (Eu (BTC)-MPS@MIP) was synthesized on the surface of silanized europium-based metal-organic frameworks (Eu (BTC)-MPS) using 1, 3, 5-benzotrioic acid (H
Language	English
Publishing date	2024-03-04
Publishing country	Austria
Document type	Journal Article
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-024-06217-3
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Article ; Online: Protective Effect of Anthocyanins against Neurodegenerative Diseases through the Microbial-Intestinal-Brain Axis: A Critical Review.

Zhong, Hao / Xu, Jie / Yang, Mengyu / Hussain, Muhammad / Liu, Xiaofeng / Feng, Fengqin / Guan, Rongfa

Nutrients

2023 Volume 15, Issue 3

Abstract: With the increase in human mean age, the prevalence of neurodegenerative diseases (NDs) also rises. This negatively affects mental and physiological health. In recent years, evidence has revealed that anthocyanins could regulate the functioning of the ... ...

Abstract	With the increase in human mean age, the prevalence of neurodegenerative diseases (NDs) also rises. This negatively affects mental and physiological health. In recent years, evidence has revealed that anthocyanins could regulate the functioning of the central nervous system (CNS) through the microbiome-gut-brain axis, which provides a new perspective for treating NDs. In this review, the protective effects and mechanisms of anthocyanins against NDs are summarized, especially the interaction between anthocyanins and the intestinal microbiota, and the microbial-intestinal-brain axis system is comprehensively discussed. Moreover, anthocyanins achieve the therapeutic purpose of NDs by regulating intestinal microflora and certain metabolites (protocateic acid, vanillic acid, etc.). In particular, the inhibitory effect of tryptophan metabolism on some neurotransmitters and the induction of blood-brain barrier permeability by butyrate production has a preventive effect on NDs. Overall, it is suggested that microbial-intestinal-brain axis may be a novel mechanism for the protective effect of anthocyanins against NDs.
MeSH term(s)	Humans ; Anthocyanins/pharmacology ; Anthocyanins/metabolism ; Neurodegenerative Diseases/prevention & control ; Neurodegenerative Diseases/metabolism ; Brain/metabolism ; Gastrointestinal Microbiome/physiology
Chemical Substances	Anthocyanins
Language	English
Publishing date	2023-01-18
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu15030496
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Article ; Online: A colorimetric and fluorescent dual-mode sensor based on bifunctional G-quadruplex-hemin complex for the determination of Pb

Ye, Tai / Chen, Haohao / Bai, Long / Yuan, Min / Cao, Hui / Hao, Liling / Wu, Xiuxiu / Yin, Fengqin / Xu, Fei

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2023 Volume 309, Page(s) 123807

Abstract: Due to the threat of lead pollution to health, environmental and food safety, developing simple and fast detection methods is highly required. Whereas, traditional single-mode probe suffers from limited application scenario. In this study, a colorimetric ...

Abstract	Due to the threat of lead pollution to health, environmental and food safety, developing simple and fast detection methods is highly required. Whereas, traditional single-mode probe suffers from limited application scenario. In this study, a colorimetric and fluorometric dual-mode probe for Pb
MeSH term(s)	DNA, Catalytic ; Hemin ; Lead ; Colorimetry/methods ; Coloring Agents ; Peroxidases ; G-Quadruplexes ; Biosensing Techniques/methods
Chemical Substances	DNA, Catalytic ; Hemin (743LRP9S7N) ; Lead (2P299V784P) ; Coloring Agents ; Peroxidases (EC 1.11.1.-)
Language	English
Publishing date	2023-12-24
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2023.123807
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Article ; Online: Gabrb2

Gao, Mingzhou / Zhang, Hao / Sun, Ya / Gao, Zhan / Sun, Chunyan / Wei, Fengqin / Gao, Dongmei

Aging

2022 Volume 14, Issue 20, Page(s) 8437–8447

Abstract: Background: Premenstrual dysphoric disorder (PMDD) is a severe mood disorder with pathological changes rooted in : Methods: PMDD-like behaviors of : Results: Gabrb2: Conclusions: GABAAR β ... ...

Abstract	Background: Premenstrual dysphoric disorder (PMDD) is a severe mood disorder with pathological changes rooted in Methods: PMDD-like behaviors of Results: Gabrb2 Conclusions: GABAAR β 2
MeSH term(s)	Humans ; Female ; Animals ; Mice ; Premenstrual Dysphoric Disorder ; Pregnanolone/metabolism ; Receptors, GABA-A/genetics ; Mice, Knockout ; DNA Copy Number Variations
Chemical Substances	Pregnanolone (BXO86P3XXW) ; Receptors, GABA-A ; GABRB2 protein, human ; Gabrb2 protein, mouse
Language	English
Publishing date	2022-10-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1945-4589
ISSN (online)	1945-4589
DOI	10.18632/aging.204351
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Article ; Online: A portable multi-channel fluorescent paper-based microfluidic chip based on smartphone imaging for simultaneous detection of four heavy metals.

Yuan, Min / Li, Chen / Zheng, Yuzhu / Cao, Hui / Ye, Tai / Wu, Xiuxiu / Hao, Liling / Yin, Fengqin / Yu, Jinsong / Xu, Fei

Talanta

2023 Volume 266, Issue Pt 2, Page(s) 125112

Abstract: Due to the excessive contamination of heavy metals pollution, it is very urgent and necessary to develop a real-time detection method for the heavy metals in food. As a target sensing device, a paper-based microfluidic device (μPAD) has the advantages of ...

Abstract	Due to the excessive contamination of heavy metals pollution, it is very urgent and necessary to develop a real-time detection method for the heavy metals in food. As a target sensing device, a paper-based microfluidic device (μPAD) has the advantages of simplicity, low-cost, and portability. In this study, a self-driven microfluidic paper-based chip was first developed for the simultaneous detection of four targets. The channels on the microfluidic chip were prepared by using wax printing and automatic screen printing on the filter paper, where liquid flowed by capillary force without pump assistance. Based on the specific binding ability of aptamers to heavy metals, a "turn-on" fluorescence aptasensor for the simultaneous detection of four heavy metal ions was developed on the proposed multi-channel device via smartphone imaging. The obtained fluorescence images were digitized into RGB color values by Image J software, and an M-mode was established to realize the quantitative detection of heavy metal ions. Under optimal conditions, the limits of detection for lead(II), mercury(II), cadmium(II), and arsenic(III) were 4.20 nM, 1.70 nM, 2.04 nM, and 1.65 nM, respectively. Furthermore, the aptasensor was successfully applied to the quantitative detection of four heavy metal ions in apple and lettuce samples with recovery rates of 84.0%-104.1%.
MeSH term(s)	Smartphone ; Microfluidics ; Metals, Heavy ; Arsenic ; Cadmium ; Coloring Agents
Chemical Substances	Metals, Heavy ; Arsenic (N712M78A8G) ; Cadmium (00BH33GNGH) ; Coloring Agents
Language	English
Publishing date	2023-08-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.125112
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Article ; Online: Low-cost, portable, on-site fluorescent detection of As(III) by a paper-based microfluidic device based on aptamer and smartphone imaging.

Yuan, Min / Li, Chen / Wang, Mengxue / Cao, Hui / Ye, Tai / Hao, Liling / Wu, Xiuxiu / Yin, Fengqin / Yu, Jinsong / Xu, Fei

Mikrochimica acta

2023 Volume 190, Issue 3, Page(s) 109

Abstract: A turn-on fluorescent aptasensor based on a paper-based microfluidic chip was developed to detect arsenite via aptamer competition strategy and smartphone imaging. The chip was prepared by wax-printing hydrophilic channels on filter paper. It is portable, ...

Abstract	A turn-on fluorescent aptasensor based on a paper-based microfluidic chip was developed to detect arsenite via aptamer competition strategy and smartphone imaging. The chip was prepared by wax-printing hydrophilic channels on filter paper. It is portable, low-cost, and environmentally friendly. Double-stranded DNA consisting of aptamer and fluorescence-labeled complementary strands was immobilized on the reaction zone of the paper chip. Due to the specific strong binding between aptamer and arsenite, the fluorescent complementary strand was squeezed out and driven by capillary force to the detection area of the paper chip, so that the fluorescent signal arose in the detection area under the excitation wavelength of 488 nm. Arsenite can be quantified by using smartphone imaging and RGB image analysis. Under the optimal conditions, the paper-based microfluidic aptasensor exhibited excellent linear response over a wide range of 1 to 1000 nM, with a detection limit as low as 0.96 nM (3σ).
MeSH term(s)	Smartphone ; Arsenites ; Oligonucleotides ; Coloring Agents ; Lab-On-A-Chip Devices
Chemical Substances	arsenite (N5509X556J) ; Arsenites ; Oligonucleotides ; Coloring Agents
Language	English
Publishing date	2023-03-03
Publishing country	Austria
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-023-05693-3
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