Article: SARS vaccine based on a replication-defective recombinant vesicular stomatitis virus is more potent than one based on a replication-competent vector.
2008 Volume 376, Issue 1, Page(s) 165–172
Abstract: ... U., Rose, J. K., Lamirande, E., Vogel, L., Subbarao, K., Roberts, A., 2005. Long-term protection ... the SARS-CoV S protein provides long-term protection of immunized mice from SARS-CoV infection (Kapadia, S ...
| Abstract | A SARS vaccine based on a live-attenuated vesicular stomatitis virus (VSV) recombinant expressing the SARS-CoV S protein provides long-term protection of immunized mice from SARS-CoV infection (Kapadia, S.U., Rose, J. K., Lamirande, E., Vogel, L., Subbarao, K., Roberts, A., 2005. Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV-based vaccine. Virology 340(2), 174-82.). Because it is difficult to obtain regulatory approval of vaccine based on live viruses, we constructed a replication-defective single-cycle VSV vector in which we replaced the VSV glycoprotein (G) gene with the SARS-CoV S gene. The virus was only able to infect cells when pseudotyped with the VSV G protein. We measured the effectiveness of immunization with the single-cycle vaccine in mice. We found that the vaccine given intramuscularly induced a neutralizing antibody response to SARS-CoV that was approximately ten-fold greater than that required for the protection from SARS-CoV infection, and significantly greater than that generated by the replication-competent vector expressing SARS-CoV S protein given by the same route. Our results, along with earlier studies showing potent induction of T-cell responses by single-cycle vectors, indicate that these vectors are excellent alternatives to live-attenuated VSV. |
|---|---|
| MeSH term(s) | Animals ; Antibodies, Viral/blood ; Genetic Vectors ; Injections, Intramuscular ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/immunology ; Mice ; Mice, Inbred BALB C ; Neutralization Tests ; SARS Virus/genetics ; SARS Virus/immunology ; Spike Glycoprotein, Coronavirus ; Vesicular stomatitis Indiana virus/genetics ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/immunology ; Viral Vaccines/genetics ; Viral Vaccines/immunology ; Virus Replication/genetics ; Virus Replication/immunology |
| Chemical Substances | Antibodies, Viral ; G protein, vesicular stomatitis virus ; Membrane Glycoproteins ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; Viral Vaccines ; spike glycoprotein, SARS-CoV ; spike protein, mouse hepatitis virus |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2008-04-08 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 200425-2 |
| ISSN | 1096-0341 ; 0042-6822 |
| ISSN (online) | 1096-0341 |
| ISSN | 0042-6822 |
| DOI | 10.1016/j.virol.2008.03.002 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Ud II Zs.172: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.