Article ; Online: In vivo Electroporation of Skeletal Muscle Fibers in Mice.
2023 Volume 13, Issue 13, Page(s) e4759
Abstract: In vitro models are essential for investigating the molecular, biochemical, and cell-biological aspects of skeletal muscle. Still, models that utilize cell lines or embryonic cells do not fully recapitulate mature muscle fibers in vivo. Protein function ... ...
| Abstract | In vitro models are essential for investigating the molecular, biochemical, and cell-biological aspects of skeletal muscle. Still, models that utilize cell lines or embryonic cells do not fully recapitulate mature muscle fibers in vivo. Protein function is best studied in mature differentiated tissue, where biological context is maintained, but this is often difficult when reliable detection reagents, such as antibodies, are not commercially available. Exogenous expression of tagged proteins in vivo solves some of these problems, but this approach can be technically challenging because either a mouse must be engineered for each protein of interest or viral vectors are required for adequate levels of expression. While viral vectors can infect target cells following local administration, they carry the risk of genome integration that may interfere with downstream analyses. Plasmids are another accessible expression system, but they require ancillary means of cell penetration; electroporation is a simple physical method for this purpose that requires minimal training or specialized equipment. Here, we describe a method for in vivo plasmid expression in a foot muscle following electroporation. |
|---|---|
| Language | English |
| Publishing date | 2023-07-05 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2833269-6 |
| ISSN | 2331-8325 ; 2331-8325 |
| ISSN (online) | 2331-8325 |
| ISSN | 2331-8325 |
| DOI | 10.21769/BioProtoc.4759 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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