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  1. Article ; Online: The Current Situation and Development Prospect of Whole-Genome Screening

    Caiting Yang / Yu Lei / Tinglin Ren / Mingze Yao

    International Journal of Molecular Sciences, Vol 25, Iss 1, p

    2024  Volume 658

    Abstract: High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out ( ... ...

    Abstract High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas9-mediated genome knock-out and dCas9-based gene activation and interference. We compare these methods with previous genetic screening techniques based on RNAi and cDNA library overexpression and propose future prospects and applications for CRISPR screening.
    Keywords genome screening ; CRISPR screen ; RNAi ; cDNA ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: The Current Situation and Development Prospect of Whole-Genome Screening.

    Yang, Caiting / Lei, Yu / Ren, Tinglin / Yao, Mingze

    International journal of molecular sciences

    2024  Volume 25, Issue 1

    Abstract: High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out ( ... ...

    Abstract High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas9-mediated genome knock-out and dCas9-based gene activation and interference. We compare these methods with previous genetic screening techniques based on RNAi and cDNA library overexpression and propose future prospects and applications for CRISPR screening.
    MeSH term(s) Endonucleases ; Gene Library ; Genetic Testing ; High-Throughput Screening Assays ; Phenotype
    Chemical Substances Endonucleases (EC 3.1.-)
    Language English
    Publishing date 2024-01-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25010658
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: An electrospun three-layer nanofibrous membrane-based

    Yin, Mingyu / Liu, Xi / Li, Caiting / Liao, Deyi / Yang, Yichao / Han, Shaobo / Fan, Longfei / Zhao, Jing / Yu, Hui / Zeng, Qingguang / Wang, Da

    Chemical communications (Cambridge, England)

    2024  Volume 60, Issue 23, Page(s) 3198–3201

    Abstract: ... ...

    Abstract An
    Language English
    Publishing date 2024-03-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d4cc00083h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Cuscutae semen

    Hou, Lanwei / Yang, Liu / Zhu, Caiting / Miao, Jingyu / Zhou, Wenjuan / Tang, Yuchun / Meng, Haiwei / Liu, Shuwei

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1107781

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2023-02-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1107781
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Brain-Type Glycogen Phosphorylase (PYGB) in the Pathologies of Diseases: A Systematic Review.

    Yang, Caiting / Wang, Haojun / Shao, Miaomiao / Chu, Fengyu / He, Yuyu / Chen, Xiaoli / Fan, Jiahui / Chen, Jingwen / Cai, Qianqian / Wu, Changxin

    Cells

    2024  Volume 13, Issue 3

    Abstract: Glycogen metabolism is a form of crucial metabolic reprogramming in cells. PYGB, the brain-type glycogen phosphorylase (GP), serves as the rate-limiting enzyme of glycogen catabolism. Evidence is mounting for the association of PYGB with diverse human ... ...

    Abstract Glycogen metabolism is a form of crucial metabolic reprogramming in cells. PYGB, the brain-type glycogen phosphorylase (GP), serves as the rate-limiting enzyme of glycogen catabolism. Evidence is mounting for the association of PYGB with diverse human diseases. This review covers the advancements in PYGB research across a range of diseases, including cancer, cardiovascular diseases, metabolic diseases, nervous system diseases, and other diseases, providing a succinct overview of how PYGB functions as a critical factor in both physiological and pathological processes. We present the latest progress in PYGB in the diagnosis and treatment of various diseases and discuss the current limitations and future prospects of this novel and promising target.
    MeSH term(s) Humans ; Glycogen Phosphorylase ; Glycogen/metabolism ; Brain/metabolism
    Chemical Substances Glycogen Phosphorylase (EC 2.4.1.-) ; Glycogen (9005-79-2)
    Language English
    Publishing date 2024-02-05
    Publishing country Switzerland
    Document type Systematic Review ; Journal Article ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells13030289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Environmental regulation and global value chain division position：Analysis based on global transnational data

    Li, Feiyang / Lin, Ziyue / Huang, Liangxiong / Yang, Caiting

    Energy policy. 2022 Sept., v. 168

    2022  

    Abstract: Based on the CEPII BACI six-digit import and export product data from 2009 to 2019 and the WDI database of the World Bank, this paper uses the bilateral stochastic frontier analysis model to calculate the global value chain (GVC) bargaining power index ... ...

    Abstract Based on the CEPII BACI six-digit import and export product data from 2009 to 2019 and the WDI database of the World Bank, this paper uses the bilateral stochastic frontier analysis model to calculate the global value chain (GVC) bargaining power index of 131 countries or regions in the world, and measure their GVC division position. This paper also uses an environmental performance index to represent the intensity of environmental regulation. A panel data model is then established to test the impact of environmental regulations on GVC division position. The results show that: (1) Environmental regulation has an inverted U-shaped nonlinear effect on GVC division position, which initially increases and then decreases; (2) The mechanism is that appropriate environmental regulation can promote scientific and technological progress, but that strict environmental regulation can inhibit scientific and technological progress; (3) The effect of environmental regulation on GVC division position only occurs in regions of poorly developed countries, during the period of global economic change after 2012, and in polluting industries.
    Keywords World Bank ; databases ; econometric models ; energy policy ; environmental law ; environmental performance ; exports ; imports ; supply chain
    Language English
    Dates of publication 2022-09
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 0301-4215
    DOI 10.1016/j.enpol.2022.113101
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: E3 ubiquitin ligase CHIP interacts with transferrin receptor 1 for degradation and promotes cell proliferation through inhibiting ferroptosis in hepatocellular carcinoma.

    Shao, Miaomiao / Qi, Kangwei / Wang, Lanxin / Yu, Xiaoxuan / Zhang, Qingyu / Yu, Long / Wang, Lan / Yang, Caiting / Fan, Lu

    Cellular signalling

    2024  Volume 118, Page(s) 111148

    Abstract: Hepatocellular carcinoma (HCC) is the major form of liver malignancy with high incidence and mortality. Identifying novel biomarkers and understanding regulatory mechanisms underlying the development and progression of HCC are critical for improving ... ...

    Abstract Hepatocellular carcinoma (HCC) is the major form of liver malignancy with high incidence and mortality. Identifying novel biomarkers and understanding regulatory mechanisms underlying the development and progression of HCC are critical for improving diagnosis, treatment and patient outcomes. Carboxyl terminus of Hsc-70-interacting protein (CHIP) is a well-described U-box-type E3 ubiquitin ligase which promotes the ubiquitination and degradation of numerous tumor-associated proteins. Recent studies have shown that CHIP can play as a tumor-suppressor gene or an oncogene in different kinds of malignancies. To date, the function and mechanism of CHIP in hepatocellular carcinoma remains largely unknown. Based on TCGA data, we found that compared with high CHIP expression, the overall survival of HCC patients with low expression of CHIP was better. In addition, CHIP overexpression markedly enhanced HCC cell proliferation and colony formation. Conversely, knockdown of CHIP restrained the proliferation and colony formation of HCC cells. Meanwhile, knockdown of CHIP decreased mitochondrial cristae or ruptured outer mitochondrial membrane, promoted the accumulation of Fe
    MeSH term(s) Humans ; Carcinoma, Hepatocellular/pathology ; Cell Line, Tumor ; Cell Proliferation ; Ferroptosis ; Liver Neoplasms/pathology ; Receptors, Transferrin ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism
    Chemical Substances Receptors, Transferrin ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; STUB1 protein, human (EC 2.3.2.27) ; CD71 antigen
    Language English
    Publishing date 2024-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1002702-6
    ISSN 1873-3913 ; 0898-6568
    ISSN (online) 1873-3913
    ISSN 0898-6568
    DOI 10.1016/j.cellsig.2024.111148
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2579: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Duck hepatitis a virus: Full-length genome-based phylogenetic and phylogeographic view during 1986-2020.

    Yang, Caiting / Shah, Pir Tariq / Bahoussi, Amina Nawal / Wu, Changxin / Wang, Li / Xing, Li

    Virus research

    2023  Volume 336, Page(s) 199216

    Abstract: Duck hepatitis A virus (DHAV) is one of key pathogens for duck viral hepatitis, especially in Asian duck industry. Currently, two main genotypes (DHAV-1 and -3) exist. To explore insightfully the evolutionary character, we assessed the available 141 full- ...

    Abstract Duck hepatitis A virus (DHAV) is one of key pathogens for duck viral hepatitis, especially in Asian duck industry. Currently, two main genotypes (DHAV-1 and -3) exist. To explore insightfully the evolutionary character, we assessed the available 141 full-length genome sequences of DHAV isolated in 1986-2020 globally and divided DHAV-1 and DHAV-3 into further seven (DHAV-1 a-g) and five (DHAV-3 a-e) sub-clades, respectively. Phylogenetic and phylogeographic network analyses indicated great genetic diversity of DHAV identified in China, where the DHAV-1 cluster and DHAV-3 cluster were linked by virus strain HDHV1-BJ (GenBank ID: FJ157172.1) and Du_CH_LSD_090612 (GenBank ID: JF828995.1) via a long mutational branch and intermediate strains. Several strains previously identified as DHAV-1 according to the partial gene sequences were actually clustered within DHAV-3 in full-length genome-based analysis. Furthermore, we identified 32 recombination events across virus genome with the recombination hotspot at the 5' end and upstream of the capsid coding region. The highest variability of DHAV polyprotein was shown at the upstream region of the N terminus P-loop region, e.g., amino acids 672-716, followed by the aa 334-359 in the Capsid encoding region. The results presented here provides a robust insight into the genetic exchange patterns of DHAV genomes during the past decades, which may be used to map the evolutionary history and facilitate preventive measures of DHAVs.
    Language English
    Publishing date 2023-09-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2023.199216
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Hydrogen-Bonded Organic Framework Supporting Atomic Bi-N

    Wang, Changli / Lv, Zunhang / Liu, Yarong / Liu, Rui / Sun, Caiting / Wang, Jinming / Li, Liuhua / Liu, Xiangjian / Feng, Xiao / Yang, Wenxiu / Wang, Bo

    Angewandte Chemie (International ed. in English)

    2024  , Page(s) e202404015

    Abstract: Single atomic catalysts (SACs) offer a superior platform for studying the structure-activity relationships during electrocatalytic ... ...

    Abstract Single atomic catalysts (SACs) offer a superior platform for studying the structure-activity relationships during electrocatalytic CO
    Language English
    Publishing date 2024-03-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202404015
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  10. Article ; Online: Interface engineering of Mn

    Zhao, Jungang / Li, Caiting / Yu, Qi / Zhu, Youcai / Liu, Xuan / Li, Shanhong / Liang, Caixia / Zhang, Ying / Huang, Le / Yang, Kuang / Zhang, Ziang / Zhai, Yunbo

    Journal of hazardous materials

    2023  Volume 452, Page(s) 131249

    Abstract: Transition metal oxides have high photothermal conversion capacity and excellent thermal catalytic activity, and their photothermal catalytic ability can be further improved by reasonably inducing the photoelectric effect of semiconductors. Herein, ... ...

    Abstract Transition metal oxides have high photothermal conversion capacity and excellent thermal catalytic activity, and their photothermal catalytic ability can be further improved by reasonably inducing the photoelectric effect of semiconductors. Herein, Mn
    Language English
    Publishing date 2023-03-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1491302-1
    ISSN 1873-3336 ; 0304-3894
    ISSN (online) 1873-3336
    ISSN 0304-3894
    DOI 10.1016/j.jhazmat.2023.131249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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