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  1. Article ; Online: Lipid raft protein flotillin-1 is important for the interaction between SOS1 and H-Ras/K-Ras, leading to Ras activation.

    Jin, Hao / Koh, Minsoo / Lim, Hyesol / Yong, Hae-Young / Kim, Eun-Sook / Kim, Sun Young / Kim, Kyoungmee / Jung, Joohee / Ryu, Won-Ji / Choi, Kang-Yell / Moon, Aree

    International journal of cancer

    2023  Volume 152, Issue 9, Page(s) 1933–1946

    Abstract: ... SOS1 and H-Ras/K-Ras in breast and pancreatic cancer cells. Stable knockdown of Flot-1 reduced ... our findings suggest that Flot-1 may serve as a membrane platform for the interaction of SOS1 with H-Ras/K-Ras ... We found that Flot-1 was co-localized with H-Ras, but not with N-Ras, in lipid rafts of MDA-MB-231 human ...

    Abstract Ras mutations have been frequently observed in human cancer. Although there is a high degree of similarity between Ras isomers, they display preferential coupling in specific cancer types. The binding of Ras to the plasma membrane is essential for its activation and biological functions. The present study elucidated Ras isoform-specific interactions with the membrane and their role in Ras-mediated biological activities. We investigated the role of a lipid raft protein flotillin-1 (Flot-1) in the activations of Ras. We found that Flot-1 was co-localized with H-Ras, but not with N-Ras, in lipid rafts of MDA-MB-231 human breast cells. The amino-terminal hydrophobic domain (1-38) of Flot-1 interacted with the hypervariable region of H-Ras. The epidermal growth factor-stimulated activation of H-Ras required Flot-1 which was not necessary for that of N-Ras in breast cancer cells. Flot-1 interacted with son of sevenless (SOS)-1, which promotes the conversion of Ras-bound GDP to GTP. Notably, Flot-1 was crucial for the interaction between SOS1 and H-Ras/K-Ras in breast and pancreatic cancer cells. Stable knockdown of Flot-1 reduced the in vivo metastasis in a mouse xenograft model with human breast carcinoma cells. A tissue microarray composed of 61 human pancreatic cancer samples showed higher levels of Flot-1 expression in pancreatic tumor tissues compared to normal tissues, and a correlation between K-Ras and Flot-1. Taken together, our findings suggest that Flot-1 may serve as a membrane platform for the interaction of SOS1 with H-Ras/K-Ras in human cancer cells, presenting Flot-1 as a potential target for Ras-driven cancers.
    MeSH term(s) Humans ; Animals ; Mice ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Membrane Microdomains/metabolism ; Pancreatic Neoplasms/metabolism
    Chemical Substances flotillins ; Membrane Proteins
    Language English
    Publishing date 2023-02-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218257-9
    ISSN 1097-0215 ; 0020-7136
    ISSN (online) 1097-0215
    ISSN 0020-7136
    DOI 10.1002/ijc.34443
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    Zs.A 478: Show issues Location:
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    Zs.MO 576: Show issues

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  2. Article ; Online: Erratum: Choi, K.-H., et al. Cancer Incidence Trend in the Hebei Spirit Oil Spill Area, from 1999 to 2014: An Ecological Study. Int. J. Environ. Res. Public Health 2018, 15, 1006.

    Choi, Kyung-Hwa / Park, Myung-Sook / Ha, Mina / Hur, Jong-Il / Cheong, Hae-Kwan

    International journal of environmental research and public health

    2018  Volume 15, Issue 7

    Abstract: Due to an error during production, the legend presented in Figure 2 in the Results section of the published paper [1] were incorrect.[ ... ]. ...

    Abstract Due to an error during production, the legend presented in Figure 2 in the Results section of the published paper [1] were incorrect.[...].
    Language English
    Publishing date 2018-07-09
    Publishing country Switzerland
    Document type Journal Article ; Published Erratum
    ISSN 1660-4601
    ISSN (online) 1660-4601
    DOI 10.3390/ijerph15071448
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  3. Article: Pharmacological properties of the gastric H(+)/K(+) ATPase inhibitor, AU-461.

    Cheon, H G / Kim, H J / Mo, H K / Lee, B H / Choi, J

    Pharmacology

    2000  Volume 60, Issue 3, Page(s) 161–168

    Abstract: ... inhibited gastric H(+)/K(+) ATPase activities with IC(50) values of 12.15 and 4.20 micromol/l for rabbit and ... to the activating cation K(+). When AU-461 was examined for the in vivo antisecretory activity, we found that AU-461 ... of the antisecretory effect was about 6 h upon oral administration. AU-461 prevented dose-dependently the ulcer ...

    Abstract AU-461 (1-(2-methyl-4-methoxyphenyl)-4-[(2-hydroxyethyl)amino]-6-beta,beta, beta-trifluoroethoxy-2,3-dihydropyrrolo[3,2-c]quinoline) was tested for its ability to act as an anti-ulcer agent. AU-461 inhibited gastric H(+)/K(+) ATPase activities with IC(50) values of 12.15 and 4.20 micromol/l for rabbit and pig enzymes, respectively. The inhibition was reversible, and competitive with respect to the activating cation K(+). When AU-461 was examined for the in vivo antisecretory activity, we found that AU-461 reduced the histamine-stimulated acid secretion as well as the basal secretion in rat stomach. Duration of the antisecretory effect was about 6 h upon oral administration. AU-461 prevented dose-dependently the ulcer formation produced by either ethanol or NaOH. This protective effect was not altered by indomethacin pretreatment. In addition, the elevated plasma gastrin by the oral administration of AU-461 was returned to control by 12 h. Taken together, these results suggest that AU-461 could be developed as a new therapeutic agent for peptic ulcer disease.
    MeSH term(s) Animals ; Anti-Ulcer Agents/therapeutic use ; Enzyme Inhibitors/therapeutic use ; Gastric Acid/secretion ; Omeprazole/therapeutic use ; Proton Pump Inhibitors ; Rabbits ; Rats ; Stomach Ulcer/chemically induced ; Stomach Ulcer/drug therapy ; Swine
    Chemical Substances Anti-Ulcer Agents ; Enzyme Inhibitors ; Proton Pump Inhibitors ; Omeprazole (KG60484QX9)
    Language English
    Publishing date 2000-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 206671-3
    ISSN 1423-0313 ; 0031-7012
    ISSN (online) 1423-0313
    ISSN 0031-7012
    DOI 10.1159/000028361
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  4. Article ; Online: Promiscuous gating modifiers target the voltage sensor of K(v)7.2, TRPV1, and H(v)1 cation channels.

    Kornilov, Polina / Peretz, Asher / Lee, Yoonji / Son, Karam / Lee, Jin Hee / Refaeli, Bosmat / Roz, Netta / Rehavi, Moshe / Choi, Sun / Attali, Bernard

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2014  Volume 28, Issue 6, Page(s) 2591–2602

    Abstract: ... VGCCs to 2 structurally related nontoxin gating modifiers, NH17 and NH29, which stabilize K(v)7.2 ... in Chinese hamster ovary cells transfected with transient receptor potential vanilloid 1 (TRPV1) or K(v)7.2 channels ... that both compounds target the VSDs of TRPV1 channels, which, like vanilloids, are involved in π-π stacking, H-bonding ...

    Abstract Some of the fascinating features of voltage-sensing domains (VSDs) in voltage-gated cation channels (VGCCs) are their modular nature and adaptability. Here we examined the VSD sensitivity of different VGCCs to 2 structurally related nontoxin gating modifiers, NH17 and NH29, which stabilize K(v)7.2 potassium channels in the closed and open states, respectively. The effects of NH17 and NH29 were examined in Chinese hamster ovary cells transfected with transient receptor potential vanilloid 1 (TRPV1) or K(v)7.2 channels, as well as in dorsal root ganglia neurons, using the whole-cell patch-clamp technique. NH17 and NH29 exert opposite effects on TRPV1 channels, operating, respectively, as an activator and a blocker of TRPV1 currents (EC50 and IC50 values ranging from 4 to 40 μM). Combined mutagenesis, electrophysiology, structural homology modeling, molecular docking, and molecular dynamics simulation indicate that both compounds target the VSDs of TRPV1 channels, which, like vanilloids, are involved in π-π stacking, H-bonding, and hydrophobic interactions. Reflecting their promiscuity, the drugs also affect the lone VSD proton channel mVSOP. Thus, the same gating modifier can promiscuously interact with different VGCCs, and subtle differences at the VSD-ligand interface will dictate whether the gating modifier stabilizes channels in either the closed or the open state.
    MeSH term(s) Animals ; CHO Cells ; Cricetinae ; Cricetulus ; Diclofenac/analogs & derivatives ; Diclofenac/pharmacology ; Diphenylamine/analogs & derivatives ; Diphenylamine/pharmacology ; Ganglia, Spinal/drug effects ; Ganglia, Spinal/physiology ; Ion Channel Gating/drug effects ; Ion Channels/metabolism ; KCNQ2 Potassium Channel/metabolism ; Molecular Dynamics Simulation ; Patch-Clamp Techniques ; Rats ; TRPV Cation Channels/metabolism
    Chemical Substances Ion Channels ; KCNQ2 Potassium Channel ; NH17 compound ; NH29 compound ; TRPV Cation Channels ; Trpv1 protein, rat ; Diclofenac (144O8QL0L1) ; Diphenylamine (9N3CBB0BIQ)
    Language English
    Publishing date 2014-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.14-250647
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    Zs.A 2266: Show issues Location:
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    Zs.MO 296: Show issues

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  5. Article ; Online: Effects of the histamine H(1) receptor antagonist hydroxyzine on hERG K(+) channels and cardiac action potential duration.

    Lee, Byung Hoon / Lee, Seung Ho / Chu, Daehyun / Hyun, Jin Won / Choe, Han / Choi, Bok Hee / Jo, Su-Hyun

    Acta pharmacologica Sinica

    2011  Volume 32, Issue 9, Page(s) 1128–1137

    Abstract: Aim: To investigate the effects of hydroxyzine on human ether-a-go-go-related gene (hERG) channels to determine the electrolphysiological basis for its proarrhythmic effects.: Methods: hERG channels were expressed in Xenopus oocytes and HEK293 cells, ...

    Abstract Aim: To investigate the effects of hydroxyzine on human ether-a-go-go-related gene (hERG) channels to determine the electrolphysiological basis for its proarrhythmic effects.
    Methods: hERG channels were expressed in Xenopus oocytes and HEK293 cells, and the effects of hydroxyzine on the channels were examined using two-microelectrode voltage-clamp and patch-clamp techniques, respectively. The effects of hydroxyzine on action potential duration were examined in guinea pig ventricular myocytes using current clamp.
    Results: Hydroxyzine (0.2 and 2 μmol/L) significantly increased the action potential duration at 90% repolarization (APD(90)) in both concentration- and time-dependent manners. Hydroxyzine (0.03-3 μmol/L) blocked both the steady-state and tail hERG currents. The block was voltage-dependent, and the values of IC(50) for blocking the steady-state and tail currents at +20 mV was 0.18±0.02 μmol/L and 0.16±0.01 μmol/L, respectively, in HEK293 cells. Hydroxyzine (5 μmol/L) affected both the activated and the inactivated states of the channels, but not the closed state. The S6 domain mutation Y652A attenuated the blocking of hERG current by ~6-fold.
    Conclusion: The results suggest that hydroxyzine could block hERG channels and prolong APD. The tyrosine at position 652 in the channel may be responsible for the proarrhythmic effects of hydroxyzine.
    MeSH term(s) Action Potentials/drug effects ; Animals ; Cell Line ; Cells, Cultured ; Ether-A-Go-Go Potassium Channels/genetics ; Ether-A-Go-Go Potassium Channels/metabolism ; Gene Expression ; Guinea Pigs ; Histamine H1 Antagonists/pharmacology ; Humans ; Hydroxyzine/pharmacology ; Myocytes, Cardiac/drug effects ; Patch-Clamp Techniques ; Xenopus laevis
    Chemical Substances Ether-A-Go-Go Potassium Channels ; Histamine H1 Antagonists ; Hydroxyzine (30S50YM8OG)
    Language English
    Publishing date 2011-09-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1360774-1
    ISSN 1745-7254 ; 0253-9756 ; 1671-4083
    ISSN (online) 1745-7254
    ISSN 0253-9756 ; 1671-4083
    DOI 10.1038/aps.2011.66
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    Zs.A 1904: Show issues Location:
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  6. Article ; Online: Proto-oncogenic H-Ras, K-Ras, and N-Ras are involved in muscle differentiation via phosphatidylinositol 3-kinase.

    Lee, Jisun / Choi, Kyu Jin / Lim, Min Jin / Hong, Feng / Choi, Tae Gyu / Tak, Eunyoung / Lee, Seonmin / Kim, Young-Joo / Chang, Sung Goo / Cho, Jin Man / Ha, Joohun / Kim, Sung Soo

    Cell research

    2010  Volume 20, Issue 8, Page(s) 919–934

    Abstract: ... we conclude that contrary to oncogenic Ras, proto-oncogenic H-Ras, K-Ras, and N-Ras are directly involved ... Oncogenic H-Ras G12V and its variants have been shown to inhibit muscle differentiation ... form of H-Ras (Ras S17N) suppressed muscle differentiation. Consistently, individual knockdown of H-Ras ...

    Abstract Oncogenic H-Ras G12V and its variants have been shown to inhibit muscle differentiation. However, the role of proto-oncogenic Ras (c-Ras) in muscle differentiation remains unclear. The active GTP-bound form of Ras has been known to associate with diverse effectors including Raf, phosphatidylinositol 3-kinase (PI3K), Ral-GDS, and other molecules to transmit downstream signals. We hypothesize that c-Ras may stimulate muscle differentiation by selectively activating PI3K, an important mediator for muscle differentiation. In our experiments, inhibition of c-Ras by farnesyltransferase inhibitors and a dominant negative form of H-Ras (Ras S17N) suppressed muscle differentiation. Consistently, individual knockdown of H-Ras, K-Ras, and N-Ras by siRNAs all blocked muscle differentiation. Interestingly, we found that c-Ras preferentially interacts with PI3K rather than its major binding partner c-Raf, during myogenic differentiation, with total c-Ras activity remaining unchanged. PI3K and its downstream myogenic pathway, the Nox2/NF-kappaB/inducible nitric oxide synthase (iNOS) pathway, were found to be suppressed by inhibition of c-Ras activity during differentiation. Furthermore, expression of a constitutively active form of PI3K completely rescued the differentiation block and reactivated the Nox2/NF-kappaB/iNOS pathway in c-Ras-inhibited cells. On the basis of our results, we conclude that contrary to oncogenic Ras, proto-oncogenic H-Ras, K-Ras, and N-Ras are directly involved in the promotion of muscle differentiation via PI3K and its downstream signaling pathways. In addition, PI3K pathway activation is associated with a concurrent suppression of the otherwise predominantly activated Raf/Mek/Erk pathway.
    MeSH term(s) Animals ; Cell Differentiation ; Cell Line ; Farnesol/analogs & derivatives ; Farnesol/pharmacology ; Membrane Glycoproteins/metabolism ; Muscle Development ; Myocardium/cytology ; NADPH Oxidase 2 ; NADPH Oxidases/metabolism ; NF-kappa B/metabolism ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins p21(ras)/genetics ; Proto-Oncogene Proteins p21(ras)/metabolism ; Proto-Oncogene Proteins p21(ras)/physiology ; RNA Interference ; RNA, Small Interfering/metabolism ; Rats ; Salicylates/pharmacology ; Signal Transduction
    Chemical Substances Membrane Glycoproteins ; NF-kappa B ; RNA, Small Interfering ; Salicylates ; farnesylthiosalicylic acid ; Nitric Oxide (31C4KY9ESH) ; Farnesol (4602-84-0) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cybb protein, rat (EC 1.6.3.-) ; NADPH Oxidase 2 (EC 1.6.3.-) ; NADPH Oxidases (EC 1.6.3.-) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
    Language English
    Publishing date 2010-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1319303-x
    ISSN 1748-7838 ; 1001-0602
    ISSN (online) 1748-7838
    ISSN 1001-0602
    DOI 10.1038/cr.2010.92
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    Zs.A 5283: Show issues Location:
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  7. Article: General pharmacological properties of YJA20379-8, a new H+/K(+)-ATPase inhibitor with anti-ulcer activities.

    Lee, E B / Cho, S I / Chang, H O / Chang, M S / Kim, K B / Lee, S B / Choi, W S

    Arzneimittel-Forschung

    2001  Volume 51, Issue 8, Page(s) 659–666

    Abstract: ... ethoxy-1,7-naphthyridine, CAS 187654-40-6), a new H+/K(+)-ATPase inhibitor with anti-ulcer activities ...

    Abstract The general pharmacological properties of YJA20379-8 (3-butyryl-4-[(R)-1-methylbenzylamino]-8-ethoxy-1,7-naphthyridine, CAS 187654-40-6), a new H+/K(+)-ATPase inhibitor with anti-ulcer activities, were investigated in mice, rats and guinea pigs. YJA20379-8 at oral doses of 25, 50 and 100 mg/kg did not affect the locomotor activity, hexobarbital hypnosis and motor coordination in mice. The drug did not have analgesic action and anticonvulsant action at the doses of 100 mg/kg p.o. The respiration and blood pressure were not affected at 10 mg/kg i.v. in rats. YJA20379-8 at 2 x 10(-4) g/ml did neither produce any contraction nor relaxation of isolated organs, such as guinea pig ileum, rat fundus, rat uterus and guinea pig vas deferens, and the drug antagonized the contractile responses to several spasmogens, such as acetylcholine, histamine, serotonin, L-phenylephrine, oxytocin and BaCl2. The drug up to 100 mg/kg p.o. did not affect pupil size and the intestinal propulsion of mice. And it did not show an anticarrageenan action at 100 mg/kg. In this general pharmacology study, hypothermic effect in mice, retardation in gastric emptying in rats, decreases in urine excretion in rats at oral doses of 50 and 100 mg/kg of YJA20379-8 and the spasmolytic activity could be found. However, no other effects were exhibited at a high oral dose of 100 mg/kg in animals in this study.
    MeSH term(s) Animals ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Anti-Ulcer Agents/pharmacology ; Autonomic Nervous System/drug effects ; Behavior, Animal/drug effects ; Cardiovascular System/drug effects ; Central Nervous System/drug effects ; Edema/chemically induced ; Edema/prevention & control ; Enzyme Inhibitors/pharmacology ; Female ; Gastrointestinal Motility/drug effects ; Guinea Pigs ; In Vitro Techniques ; Male ; Mice ; Mice, Inbred ICR ; Muscle Contraction/drug effects ; Muscle, Smooth/drug effects ; Naphthyridines/pharmacology ; Proton Pump Inhibitors ; Rats ; Rats, Sprague-Dawley ; Respiratory System/drug effects
    Chemical Substances 3-butyryl-4-(5R-methylbenzylamino)-8-ethoxy-1,7-naphthyridine ; Anti-Inflammatory Agents, Non-Steroidal ; Anti-Ulcer Agents ; Enzyme Inhibitors ; Naphthyridines ; Proton Pump Inhibitors
    Language English
    Publishing date 2001
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 502081-5
    ISSN 1616-7066 ; 0004-4172
    ISSN (online) 1616-7066
    ISSN 0004-4172
    DOI 10.1055/s-0031-1300097
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  8. Article: Altered Regulation of type 3 Na(+)/H(+) exchanger, type 1 Na(+)/HCO3 (-) cotransporter, and Na(+),K(+)-ATPase in the Kidney of Rats with Experimental Rhabdomyolysis.

    Ma, Seong Kwon / Bae, Eun Hui / Lee, Jongun / Kim, Sun Young / Kim, Sung Zoo / Choi, Ki Chul / Kim, Soo Wan

    Electrolyte & blood pressure : E & BP

    2007  Volume 5, Issue 2, Page(s) 55–61

    Abstract: ... with rhabdomyolysis. The present study was aimed to investigate whether the changes of type 3 Na(+)/H(+) exchanger ... NHE3), type 1 Na(+)/HCO3 (-) cotransporter (NBC1), and Na(+),K(+)-ATPase α1 subunit may play a role ... by decapitation. Control rats were injected with normal saline. The protein expression of NHE3, NBC1 and Na(+),K ...

    Abstract Metabolic acidosis was shown to correlate with deterioration of renal function in patients with rhabdomyolysis. The present study was aimed to investigate whether the changes of type 3 Na(+)/H(+) exchanger (NHE3), type 1 Na(+)/HCO3 (-) cotransporter (NBC1), and Na(+),K(+)-ATPase α1 subunit may play a role in the pathogenesis of metabolic acidosis in glycerol-induced experimental rhabdomyolysis. Male Sprague-Dawley rats were deprived of fluid intake for 24 hours, and then were injected with 50% glycerol in normal saline (10 mL/kg, intramuscularly). At 24 hours after the glycerol injection, rats were sacrificed by decapitation. Control rats were injected with normal saline. The protein expression of NHE3, NBC1 and Na(+),K(+)-ATPase α1 subunit was determined in the cortex of the kidney by immunoblotting and immunohistochemistry. Following the treatment of glycerol, creatinine clearance was significantly decreased, and high anion gap metabolic acidosis developed. In the experimental group, the expression of Na(+),K(+)-ATPase α1 subunit was significantly decreased in the cortex of the kidney. On the contrary, the expression of NHE3 and NBC1 was significantly increased. Immunohistochemical analyses confirmed the immunoblotting data. In conclusion, the coordinate up-regulation of NHE3 and NBC1 may play an adaptive role against the metabolic acidosis in glycerol-induced rhabdomyolysis.
    Language English
    Publishing date 2007-12-31
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 3017960-9
    ISSN 2092-9935 ; 1738-5997
    ISSN (online) 2092-9935
    ISSN 1738-5997
    DOI 10.5049/EBP.2007.5.2.55
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  9. Article: UV irradiation effects on the bonding structure and electrical properties of ultra low-k SiOC(–H) thin films for 45 nm technology node

    Choi, Chi Kyu / Kim, Chang Young / Navamathavan, R / Lee, Heang Seuk / Woo, Jong-Kwan / Hyun, Myung Taek / Lee, Heon Ju / Jeung, Won Young

    Current applied physics. 2011 Sept., v. 11, no. 5S

    2011  

    Abstract: Low-dielectric-constant SiOC(–H) thin films were deposited on p-type Si(100) substrates using ... oxygen gas as precursors. To improve the structural, mechanical and electrical characteristics, SiOC(–H ... Carbon content of the SiOC(–H) films increased before 240 s of UV irradiation time. But carbon-bonded functional ...

    Abstract Low-dielectric-constant SiOC(–H) thin films were deposited on p-type Si(100) substrates using plasma enhanced chemical vapor deposition (PECVD) from vinyltrimethylsilane (VTMS; CH₂ = CHSi(CH₃)₃) and oxygen gas as precursors. To improve the structural, mechanical and electrical characteristics, SiOC(–H) films deposited using PECVD were post-treated by ultraviolet (UV) irradiation for various time intervals. Carbon content of the SiOC(–H) films increased before 240 s of UV irradiation time. But carbon-bonded functional groups of the SiOC(–H) film, in case of 480 s UV irradiation time, is replaced with Si–O bond. Because the Si–CHₙ bond groups are broken due to UV irradiation, Therefore, the films are formed with Si–O bond rich in the Si–O–C(–H) structure. The lowest relative dielectric constant, leakage current density, the elastic modulus and the hardness of SiOC(–H) with 240 s of UV irradiation time were about 2.07, 2.1 × 10⁻⁷A/cm², 43 GPa, and 3.68 GPa, respectively. The results indicate that the SiOC(–H) films exposed by UV irradiation improve the structural, mechanical, and electrical characteristics.
    Keywords carbon ; electrical properties ; hardness ; irradiation ; modulus of elasticity ; oxygen ; silicon ; ultraviolet radiation ; vapors
    Language English
    Dates of publication 2011-09
    Size p. S109-S113.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 1567-1739
    DOI 10.1016/j.cap.2011.05.004
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Pharmacological Properties of the Gastric H ; /K ; ATPase Inhibitor, AU-461

    Cheon, Hyae Gyeong / Kim, Hyun Jung / Mo, Hye Kyoung / Lee, Byoung Ho / Choi, Joong-Kwon

    Pharmacology

    2000  Volume 60, Issue 3, Page(s) 161–168

    Abstract: ... gastric H/K ATPase activities with IC50 values of 12.15 and 4.20 μmol/l for rabbit and pig enzymes ... respectively. The inhibition was reversible, and competitive with respect to the activating cation K. When AU ... was about 6 h upon oral administration. AU-461 prevented dose-dependently the ulcer formation produced ...

    Institution Pharmaceutical Screening Center, and Bio-Organic Science Division, Korea Research Institute of Chemical Technology, TaeJon, Korea
    Abstract AU-461 (1-(2-methyl-4-methoxyphenyl)-4-[(2-hydroxyethyl)amino]-6-β,β,β-trifluoroethoxy-2,3-dihydropyrrolo[3,2-c]quinoline) was tested for its ability to act as an anti-ulcer agent. AU-461 inhibited gastric H/K ATPase activities with IC50 values of 12.15 and 4.20 μmol/l for rabbit and pig enzymes, respectively. The inhibition was reversible, and competitive with respect to the activating cation K. When AU-461 was examined for the in vivo antisecretory activity, we found that AU-461 reduced the histamine-stimulated acid secretion as well as the basal secretion in rat stomach. Duration of the antisecretory effect was about 6 h upon oral administration. AU-461 prevented dose-dependently the ulcer formation produced by either ethanol or NaOH. This protective effect was not altered by indomethacin pretreatment. In addition, the elevated plasma gastrin by the oral administration of AU-461 was returned to control by 12 h. Taken together, these results suggest that AU-461 could be developed as a new therapeutic agent for peptic ulcer disease.
    Keywords H ; /K ; ATPase ; Gastric acid secretion ; Antiulcer agent ; Plasma gastrin ; Duration of action
    Language English
    Publishing date 2000-04-05
    Publisher S. Karger AG
    Publishing place Basel, Switzerland
    Document type Article
    Note Original Paper
    ZDB-ID 206671-3
    ISSN 1423-0313 ; 0031-7012
    ISSN (online) 1423-0313
    ISSN 0031-7012
    DOI 10.1159/000028361
    Database Karger publisher's database

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