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Article ; Online: Longitudinal assessment of coagulation potential before, during, and following an in vitro fertilization cycle.

Nesbit, Carleigh / Gunalp, Cemile / Zhang, Jiahui / McGee, Elizabeth / Bernstein, Ira / Orfeo, Thomas / Bravo, Maria Cristina

Thrombosis research

2024 Volume 238, Page(s) 97–102

Abstract: Introduction: The association between estrogen and hypercoagulability is well-established but little is known about coagulation dynamics during IVF. Our goal was to measure coagulation potential prior to, during, and following an IVF cycle and to ... ...

Abstract	Introduction: The association between estrogen and hypercoagulability is well-established but little is known about coagulation dynamics during IVF. Our goal was to measure coagulation potential prior to, during, and following an IVF cycle and to investigate differences by conception outcome. Materials and methods: Patients undergoing IVF with fresh embryo transfer at a single academic center using oral contraceptive pills for cycle batching underwent evaluation of thrombin generation using the calibrated automated thrombogram at multiple points during the IVF cycle. Multiple thrombin generation parameters were compared across timepoints and by IVF cycle outcome using ANOVA repeated measures analysis. Results: Of the 17 patients included, 11 conceived. There was a significant increase in peak and total thrombin generation in the entire cohort between the pre-treatment natural follicular phase and following a short course of oral contraceptive pills used for cycle batching. Further increase in these parameters was seen at the time of oocyte retrieval. In the pre-treatment natural follicular phase, patients who conceived had lower peak thrombin generation. There were changes throughout the cycle for factors II, V, VIII, X, XI, XII, antithrombin, and tissue factor pathway inhibitor. Only Factor XI was distinguishable by conception status; values were lower at all visits in patients who conceived. Conclusion: Increases in coagulation potential are seen in patients undergoing IVF following a short course of oral contraceptive pills for cycle batching and continue during controlled ovarian hyperstimulation. Those who conceived were seen to have lower peak thrombin generation in the pre-treatment natural follicular phase.
Language	English
Publishing date	2024-04-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	121852-9
ISSN	1879-2472 ; 0049-3848
ISSN (online)	1879-2472
ISSN	0049-3848
DOI	10.1016/j.thromres.2024.04.020
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Article ; Online: Identification of fungi isolated from commercial bumblebee colonies.

Chow, Lui Julie / Nesbit, Miles L / Hill, Tom / Tranter, Christopher / Evison, Sophie E F / Hughes, William O H / Graystock, Peter

PeerJ

2024 Volume 12, Page(s) e16713

Abstract: Fungi can have important beneficial and detrimental effects on animals, yet our understanding of the diversity and function of most bee-associated fungi is poor. Over 2 million bumblebee colonies are traded globally every year, but the presence and ... ...

Abstract	Fungi can have important beneficial and detrimental effects on animals, yet our understanding of the diversity and function of most bee-associated fungi is poor. Over 2 million bumblebee colonies are traded globally every year, but the presence and transport of viable fungi within them is unknown. Here, we explored whether any culturable fungi could be isolated from commercial bumblebee nests. We collected samples of various substrates from within 14 bumblebee colonies, including the honey, honey cup wall, egg cup wall, and frass then placed them on agar and recorded any growth. Fungal morphotypes were then subcultured and their ITS region sequenced for identification. Overall, we cultured 11 fungal species from the various nest substrates. These included both pathogenic and non-pathogenic fungi, such as
MeSH term(s)	Bees ; Animals ; Aspergillus ; Penicillium ; Agar
Chemical Substances	Agar (9002-18-0)
Language	English
Publishing date	2024-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.16713
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Article: Building Local Capacity in a Low-Resource Setting to Increase Access to Health Care: An Evaluation of Blood Pressure Monitoring Training.

Weir, Sarah / May, Cassandra / Wills, Alexa / Van Zanten, Erin / Nesbit, Kathryn / Ngalande, Alexander / Kanjirawaya, Ruth

Health promotion practice

2024 , Page(s) 15248399231225444

Abstract: Cardiovascular risk factors such as hypertension are common and largely uncontrolled in Malawi. In this low-resource setting, Community Health Workers (CHWs) can increase access to home-based blood pressure (BP) monitoring. The purpose of this study is ... ...

Abstract	Cardiovascular risk factors such as hypertension are common and largely uncontrolled in Malawi. In this low-resource setting, Community Health Workers (CHWs) can increase access to home-based blood pressure (BP) monitoring. The purpose of this study is to evaluate the effectiveness of a CHW training focused on BP monitoring and referral criteria, as well as the accuracy of referral decision-making and documentation. The participants were a purposive sample of all active home-based palliative care CHWs at St. Gabriel's Hospital (n = 60) located in Namitete, Malawi, serving over 250,000 people within a 50 km radius. This was a retrospective cross-sectional study conducted in December 2020 using both quantitative (descriptive, paired t-test) and qualitative (thematic) analysis. Participants showed significantly greater knowledge on the post-test (M = 8.98, SD = 1.213) compared to the pretest (M = 7.96, SD = 1.231), t (54)-5.0557.475, p < .001. All participants who attended both days of training demonstrated competency on a skills checklist in 100% of the rehabilitation and BP monitoring skills taught. Through document analysis of record books, referral decisions for patients with hypertension were 87.57% accurate and 81.07% of entries (n = 713) were complete. Participants reported the lack of both transportation and equipment as barriers to their work. They reported trainings, supplies, and support from the hospital as facilitators to their work. This study shows that BP can be monitored in remote villages, accurate referrals can be made, and stroke prevention education can be provided. These interventions increase the chances of more equitable care for this vulnerable population in a resource-limited setting.
Language	English
Publishing date	2024-02-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2036801-X
ISSN	1552-6372 ; 1524-8399
ISSN (online)	1552-6372
ISSN	1524-8399
DOI	10.1177/15248399231225444
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Article: Peripheral metabolism of lipoprotein-amyloid beta as a risk factor for Alzheimer's disease: potential interactive effects of APOE genotype with dietary fats.

D'Alonzo, Zachary J / Lam, Virginie / Takechi, Ryu / Nesbit, Michael / Vaccarezza, Mauro / Mamo, John C L

Genes & nutrition

2023 Volume 18, Issue 1, Page(s) 2

Abstract: ... risk. Similarly, apolipoprotein E (Apo E) serves as the primary ligand by which lipoproteins are ...

Abstract	Alzheimer's disease (AD) is a progressive neurodegenerative disorder pathologically characterized by brain parenchymal abundance of amyloid-beta (Aβ) and the accumulation of lipofuscin material that is rich in neutral lipids. However, the mechanisms for aetiology of AD are presently not established. There is increasing evidence that metabolism of lipoprotein-Aβ in blood is associated with AD risk, via a microvascular axis that features breakdown of the blood-brain barrier, extravasation of lipoprotein-Aβ to brain parenchyme and thereafter heightened inflammation. A peripheral lipoprotein-Aβ/capillary axis for AD reconciles alternate hypotheses for a vascular, or amyloid origin of disease, with amyloidosis being probably consequential. Dietary fats may markedly influence the plasma abundance of lipoprotein-Aβ and by extension AD risk. Similarly, apolipoprotein E (Apo E) serves as the primary ligand by which lipoproteins are cleared from plasma via high-affinity receptors, for binding to extracellular matrices and thereafter for uptake of lipoprotein-Aβ via resident inflammatory cells. The epsilon APOE ε4 isoform, a major risk factor for AD, is associated with delayed catabolism of lipoproteins and by extension may increase AD risk due to increased exposure to circulating lipoprotein-Aβ and microvascular corruption.
Language	English
Publishing date	2023-02-25
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2416599-2
ISSN	1865-3499 ; 1555-8932
ISSN (online)	1865-3499
ISSN	1555-8932
DOI	10.1186/s12263-023-00722-5
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Article: Perioperative methadone prescribing and association with respiratory depression.

Bova, Sarah E / Kruer, Rachel M / Nesbit, Suzanne A / Grant, Michael C / Jarrell, Andrew S

Journal of opioid management

2021 Volume 16, Issue 6, Page(s) 443–449

Abstract: Objective: Over 80 percent of surgery patients experience acute post-operative pain and less than half feel their pain is adequately controlled. Patients receiving chronic opioids, including methadone, are at the highest risk of inadequate pain control. ...

Abstract	Objective: Over 80 percent of surgery patients experience acute post-operative pain and less than half feel their pain is adequately controlled. Patients receiving chronic opioids, including methadone, are at the highest risk of inadequate pain control. Guidelines do not provide specific recommendations for analgesia management in this population. The purpose of this study was to evaluate the association between post-operative methadone use and respiratory depression. Design: This study was a single center, retrospective, cohort study of adult patients. Setting: Patients included were admitted to a single academic medical center from July 2016 to September 2018. Participants: Medical records of adult inpatients with an operative procedure who received perioperative methadone were reviewed. Main outcome measures: Preoperative methadone use was evaluated for all patients. Post-operative methadone dosing was compared to preoperative methadone dosing. Post-operative respiratory depression was evaluated. Logistic regression was performed to identify risk factors for respiratory depression. Results: Two hundred ninety-eight patients were included in the study. Patients were divided into groups based on pre-operative methadone use. Over 90 percent of patients were on preoperative methadone. There were no significant differences in baseline characteristics between groups. In the initial seven post-operative days, 14.8 percent of patients had documented respiratory depression. Respiratory depression was more common among patients who were newly initiated on methadone post-operatively. Factors associated with respiratory depression included male sex, increased age, and new post-operative methadone initiation. Conclusions: Most patients who were administered post-operative methadone were on preoperative methadone. New post-operative methadone initiation was a risk factor for respiratory depression.
MeSH term(s)	Adult ; Analgesics, Opioid/administration & dosage ; Analgesics, Opioid/adverse effects ; Female ; Humans ; Male ; Methadone/administration & dosage ; Methadone/adverse effects ; Pain, Postoperative/drug therapy ; Perioperative Period ; Respiratory Insufficiency/chemically induced ; Respiratory Insufficiency/diagnosis ; Respiratory Insufficiency/epidemiology ; Retrospective Studies ; Surgical Procedures, Operative
Chemical Substances	Analgesics, Opioid ; Methadone (UC6VBE7V1Z)
Language	English
Publishing date	2021-01-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2397614-7
ISSN	1551-7489
ISSN	1551-7489
DOI	10.5055/jom.2020.0602
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Zs.A 6563: Show issues

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Article ; Online: Effect of Drug Disposal Kits and Fact Sheets on Elimination of Leftover Prescription Opioids: The DISPOSE Multi-Arm Randomized Controlled Trial.

Bicket, Mark C / Fu, Denise / Swarthout, Meghan D / White, Elizabeth / Nesbit, Suzanne A / Monitto, Constance L

Pain medicine (Malden, Mass.)

2021 Volume 22, Issue 4, Page(s) 961–969

Abstract: Objective: To determine how passively providing informational handouts and/or drug disposal kits affects rates of leftover prescription opioid disposal.: Design: A multi-arm parallel-group randomized controlled trial with masked outcome assessment ... ...

Abstract	Objective: To determine how passively providing informational handouts and/or drug disposal kits affects rates of leftover prescription opioid disposal. Design: A multi-arm parallel-group randomized controlled trial with masked outcome assessment and computer-guided randomization. Setting: Johns Hopkins Health System outpatient pharmacies. Subjects: Individuals who filled ≥1 short-term prescription for an immediate-release opioid for themselves or a family member. Methods: In June 2019, 499 individuals were randomized to receive an informational handout detailing U.S. Food and Drug Administration-recommended ways to properly dispose of leftover opioids (n = 188), the informational handout and a drug disposal kit with instructions on its use (n = 170), or no intervention (n = 141) at prescription pickup. Subjects were subsequently contacted by telephone, and outcomes were assessed by a standardized survey. The primary outcome was the use of a safe opioid disposal method. Results: By 6 weeks after prescription pickup, 227 eligible individuals reported they had stopped taking prescription opioids to treat pain and had leftover medication. No difference in safe disposal was observed between the non-intervention group (10% [6/63]) and the group that received disposal kits (14% [10/73]) (risk ratio = 1.44; 95% confidence interval: 0.55 to 3.74) or the group that received a fact sheet (11% [10/91]) (risk ratio = 1.15; 95% confidence interval: 0.44 to 3.01). Conclusions: These findings suggest that passive provision of a drug disposal kit at prescription pickup did not increase rates of leftover opioid disposal when compared with provision of a fact sheet alone or no intervention. Active interventions may deserve further investigation.
MeSH term(s)	Analgesics, Opioid/therapeutic use ; Drug Prescriptions ; Family ; Humans ; Pharmaceutical Preparations ; Surveys and Questionnaires
Chemical Substances	Analgesics, Opioid ; Pharmaceutical Preparations
Language	English
Publishing date	2021-03-04
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	2015903-1
ISSN	1526-4637 ; 1526-2375
ISSN (online)	1526-4637
ISSN	1526-2375
DOI	10.1093/pm/pnaa431
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Zs.A 6119: Show issues

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Article ; Online: Technology and rehabilitation training for community health workers: Strengthening health systems in Malawi.

Wilson, Ellen / Lee, Lydia / Klas, Rachel / Nesbit, Kathryn C

Health & social care in the community

2019 Volume 28, Issue 3, Page(s) 833–841

Abstract: Community health workers (CHWs) have been effectively utilised in resource-limited settings to combat a growing demand for health access that cannot be met by the current workforce. The purpose of this study was to evaluate a CHW training programme in ... ...

Abstract	Community health workers (CHWs) have been effectively utilised in resource-limited settings to combat a growing demand for health access that cannot be met by the current workforce. The purpose of this study was to evaluate a CHW training programme in Malawi that integrated technology into rehabilitation care delivery. This was a retrospective cross-sectional study of a training programme conducted in December 2018. The participants were a convenience sample of all active home-based palliative care CHWs at St. Gabriel's Hospital (n = 60). The data collected included the following: a written pre- and post-knowledge test, skills competency checklist and a post-training programme survey. Descriptive frequencies described skill competency and quantitative responses from the post-training programme survey. Paired t test (α = 0.05) analysis determined the significance of knowledge acquisition. Themes in the narrative responses in the post-training survey were identified. Both training programme groups showed significantly greater knowledge on the post-test (M = 9.50, SD = 0.861; M = 9.43, SD = 0.971) compared to the pre-test (M = 7.97, SD = 1.351; M = 7.90, SD = 1.900); t(29) = 6.565, p < .001; t(29) = 4.104, p < .001 for Group 1 and Group 2, respectively. All participants demonstrated skill competency in 100% of the skills. All participants responded that the training programme helped them review skills and understand how to use technology 'A lot' on a Likert scale (no, a little, some, a lot). Facilitators of their work included training programmes, phones, communication with the hospital and collaboration amongst CHWs. Barriers included transportation needs, lack of patient care supplies and lack of caregiver compliance. Overall, utilisation of their knowledge and skills from the training programmes helped their patients make improvements in mobility and function that are meaningful to their quality of life in the village. This study highlights the importance of assessing programmes in low-resource settings with a focus on feasibility and developing local capacity.
MeSH term(s)	Adult ; Clinical Competence ; Community Health Workers/education ; Cross-Sectional Studies ; Developing Countries ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Malawi ; Male ; Middle Aged ; Quality of Life ; Retrospective Studies ; Technology
Language	English
Publishing date	2019-12-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1155902-0
ISSN	1365-2524 ; 0966-0410
ISSN (online)	1365-2524
ISSN	0966-0410
DOI	10.1111/hsc.12914
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Article: An evidence-based approach to managing acute otitis media.

Nesbit, Chadd E / Powers, Margaret C

Pediatric emergency medicine practice

2013 Volume 10, Issue 4, Page(s) 1–26; quiz 26–7

Abstract: Acute otitis media is one of the most common pediatric illnesses; however, there is considerable controversy in its management. While most cases are treated with antibiotics, there is a growing concern regarding antibiotic overuse and subsequent drug ... ...

Abstract	Acute otitis media is one of the most common pediatric illnesses; however, there is considerable controversy in its management. While most cases are treated with antibiotics, there is a growing concern regarding antibiotic overuse and subsequent drug resistance. Researchers in the Netherlands have developed a "watchful waiting" (ie, an observation approach) that has been successful in treating acute otitis media, although it has not gained widespread popularity in the United States. This review will summarize the latest research on diagnosing acute otitis media as well as different treatment regimens, including the efficacy of the watchful-waiting approach.
MeSH term(s)	Acute Disease ; Anti-Bacterial Agents/therapeutic use ; Child ; Critical Pathways ; Diagnosis, Differential ; Dose-Response Relationship, Drug ; Emergency Service, Hospital ; Histamine Antagonists/therapeutic use ; Humans ; Mastoiditis/etiology ; Meningitis/etiology ; Middle Ear Ventilation ; Nasal Decongestants/therapeutic use ; Otitis Media/complications ; Otitis Media/diagnosis ; Otitis Media/etiology ; Otitis Media/therapy ; Otitis Media, Suppurative/therapy ; Otoscopy ; Pain Management ; Physical Examination ; Practice Guidelines as Topic ; Risk Management ; Watchful Waiting
Chemical Substances	Anti-Bacterial Agents ; Histamine Antagonists ; Nasal Decongestants
Keywords	covid19
Language	English
Publishing date	2013-04
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1549-9650
ISSN	1549-9650
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Article ; Online: Assessment of Indices of Conjunctival Microvascular Function in Patients With and Without Obstructive Coronary Artery Disease.

Mailey, Jonathan A / Moore, Julie S / Brennan, Paul F / Jing, Min / Awuah, Agnes / Trucco, Emanuele / McLaughlin, James A D / Nesbit, M Andrew / Moore, Tara C B / Spence, Mark S

Cardiovascular revascularization medicine : including molecular interventions

2023 Volume 50, Page(s) 26–33

Abstract: Background: Atherosclerotic heart disease often remains asymptomatic until presentation with a major adverse cardiovascular event. Primary preventive therapies improve outcomes, but conventional screening often misattributes risk. Vascular imaging can ... ...

Abstract	Background: Atherosclerotic heart disease often remains asymptomatic until presentation with a major adverse cardiovascular event. Primary preventive therapies improve outcomes, but conventional screening often misattributes risk. Vascular imaging can be utilised to detect atherosclerosis, but often involves ionising radiation. The conjunctiva is a readily accessible vascular network allowing non-invasive hemodynamic evaluation. Aim: To compare conjunctival microcirculatory function in patients with and without obstructive coronary artery disease. Methods: We compared the conjunctival microcirculation of myocardial infarction patients (MI-cohort) to controls with no obstructive coronary artery disease (NO-CAD cohort). Conjunctival imaging was performed using a smartphone and slit-lamp biomicroscope combination. Microvascular indices of axial (V Results: A total of 127 patients were recruited (66 MI vs 61 NO-CAD) and 3602 conjunctival vessels analysed (2414 MI vs 1188 NO-CAD). Mean V Conclusions: Conjunctival microcirculatory alterations can be observed in patients with obstructive coronary artery disease. The conjunctival microvasculature merits further evaluation in cardiovascular risk screening.
MeSH term(s)	Humans ; Coronary Artery Disease/diagnostic imaging ; Blood Flow Velocity ; Microcirculation/physiology ; Cross-Sectional Studies ; Conjunctiva/blood supply ; Coronary Vessels/diagnostic imaging ; Coronary Angiography
Language	English
Publishing date	2023-01-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2212113-4
ISSN	1878-0938 ; 1553-8389
ISSN (online)	1878-0938
ISSN	1553-8389
DOI	10.1016/j.carrev.2023.01.007
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Article: Chemotherapeutic Activity of Pitavastatin in Vincristine Resistant B-Cell Acute Lymphoblastic Leukemia.

Piktel, Debbie / Moore, Javohn C / Nesbit, Sloan / Sprowls, Samuel A / Craig, Michael D / Rellick, Stephanie L / Nair, Rajesh R / Meadows, Ethan / Hollander, John M / Geldenhuys, Werner J / Martin, Karen H / Gibson, Laura F

Cancers

2023 Volume 15, Issue 3

Abstract: B-cell acute lymphoblastic leukemia (ALL) is derived from an accumulation of malignant, immature B cells in the bone marrow and blood. Relapse due, in part, to the emergence of tumor cells that are resistant to front line standard chemotherapy is ... ...

Abstract	B-cell acute lymphoblastic leukemia (ALL) is derived from an accumulation of malignant, immature B cells in the bone marrow and blood. Relapse due, in part, to the emergence of tumor cells that are resistant to front line standard chemotherapy is associated with poor patient outcomes. This challenge highlights the need for new treatment strategies to eliminate residual chemoresistant tumor cells. Based on the use of pitavastatin in acute myeloid leukemia (AML), we evaluated its efficacy in an REH ALL cell line derived to be resistant to vincristine. We found that pitavastatin inhibited the proliferation of both parental and vincristine-resistant REH tumor cells at an IC
Language	English
Publishing date	2023-01-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15030707
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