Article ; Online: Characteristics of alpha smooth muscle actin-positive peritubular cells in prepubertal bovine testes.
Biochemical and biophysical research communications
2022 Volume 609, Page(s) 48–53
Abstract: Characteristics of peritubular myoid cells (PMCs) in sexually immature cattle remain largely unknown. Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (αSMA), a marker of PMCs in adult testes, in ... ...
Abstract | Characteristics of peritubular myoid cells (PMCs) in sexually immature cattle remain largely unknown. Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (αSMA), a marker of PMCs in adult testes, in prepubertal testes procured from 5-months-old bulls. Elastin distribution around αSMA + PMCs was dim and discontinuous in prepubertal testes, but strong and continuous in adult testes. Fibroblast-specific protein 1 (FSP1) was rarely expressed in αSMA + PMCs of prepubertal testes, while in adult testes, majority of αSMA + PMCs were FSP1+. Moreover, αSMA + PMCs in prepubertal testes proliferated more actively than those in adult testes. In vitro culture of isolated seminiferous tubules from prepubertal testes revealed that αSMA + PMCs migrate from peritubular to interstitial area. Hence, in prepubertal bovine testes, (1) PMCs rarely portray fibroblast-like properties, (2) PMCs exhibit heterogeneity in FSP1 expression, (3) PMCs proliferate more actively than those in adult testes, and (4) PMCs have a potential to migrate to the interstitium. Our observations help to understand the maturation of PMCs and their involvement in bovine testicular function. |
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MeSH term(s) | Actins/metabolism ; Animals ; Cattle ; Fibroblasts/metabolism ; Male ; Muscle, Smooth/metabolism ; Seminiferous Tubules/metabolism ; Testis/metabolism |
Chemical Substances | Actins |
Language | English |
Publishing date | 2022-04-07 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 205723-2 |
ISSN | 1090-2104 ; 0006-291X ; 0006-291X |
ISSN (online) | 1090-2104 ; 0006-291X |
ISSN | 0006-291X |
DOI | 10.1016/j.bbrc.2022.03.149 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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