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  1. Book: Fungal genomics

    Dunlap, Jay C.

    (Advances in genetics ; 57)

    2007  

    Author's details ed. by Jay C. Dunlap
    Series title Advances in genetics ; 57
    Collection
    Language English
    Size XIII, 302 S., [2] Bl. : Ill., graph. Darst.
    Publisher Elsevier
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT015083757
    ISBN 0-12-017657-2 ; 978-0-12-017657-1
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Shelf markLoan statusLocation
    088/511 available for loan (reading room) Freihandmagazin (U1)

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  2. Book: Fungal genomics

    Dunlap, Jay C.

    (Advances in genetics ; 57)

    2007  

    Author's details ed. by Jay C. Dunlap
    Series title Advances in genetics ; 57
    Collection
    Language English
    Size XIII, 302 S., [2] Bl. : Ill., graph. Darst.
    Publisher Elsevier
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT015083757
    ISBN 0-12-017657-2 ; 978-0-12-017657-1
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Se 836 -57- verfügbar in Königswinter Königswinter

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  3. Article: Domains Required for FRQ-WCC Interaction within the Core Circadian Clock of

    Wang, Bin / Dunlap, Jay C

    bioRxiv : the preprint server for biology

    2023  

    Abstract: In the negative feedback loop composing ... ...

    Abstract In the negative feedback loop composing the
    Language English
    Publishing date 2023-02-26
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.25.530043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Inter-library loan at ZB MED

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  4. Book: Computational methods for genetics of complex traits

    Moore, Jason H. / Dunlap, Jay C.

    (Advances in genetics ; 72)

    2010  

    Author's details ed. by Jay C. Dunlap ; Jason H. Moore
    Series title Advances in genetics ; 72
    Collection
    Language English
    Size X, 200 S. : Ill., graph. Darst.
    Edition 1. ed.
    Publisher Elsevier
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT016595534
    ISBN 978-0-12-380862-2 ; 0-12-380862-6
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Se 836 -72- verfügbar in Königswinter Königswinter

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  5. Article ; Online: Domains required for the interaction of the central negative element FRQ with its transcriptional activator WCC within the core circadian clock of Neurospora.

    Wang, Bin / Dunlap, Jay C

    The Journal of biological chemistry

    2023  Volume 299, Issue 7, Page(s) 104850

    Abstract: In the negative feedback loop composing the Neurospora circadian clock, the core element, FREQUENCY (FRQ), binds with FRQ-interacting RNA helicase (FRH) and casein kinase 1 to form the FRQ-FRH complex (FFC) which represses its own expression by ... ...

    Abstract In the negative feedback loop composing the Neurospora circadian clock, the core element, FREQUENCY (FRQ), binds with FRQ-interacting RNA helicase (FRH) and casein kinase 1 to form the FRQ-FRH complex (FFC) which represses its own expression by interacting with and promoting phosphorylation of its transcriptional activators White Collar-1 (WC-1) and WC-2 (together forming the White Collar complex, WCC). Physical interaction between FFC and WCC is a prerequisite for the repressive phosphorylations, and although the motif on WCC needed for this interaction is known, the reciprocal recognition motif(s) on FRQ remains poorly defined. To address this, we assessed FFC-WCC in a series of frq segmental-deletion mutants, confirming that multiple dispersed regions on FRQ are necessary for its interaction with WCC. Biochemical analysis shows that interaction between FFC and WCC but not within FFC or WCC can be disrupted by high salt, suggesting that electrostatic forces drive the association of the two complexes. As a basic sequence on WC-1 was previously identified as a key motif for WCC-FFC assembly, our mutagenetic analysis targeted negatively charged residues of FRQ, leading to identification of three Asp/Glu clusters in FRQ that are indispensable for FFC-WCC formation. Surprisingly, in several frq Asp/Glu-to-Ala mutants that vastly diminish FFC-WCC interaction, the core clock still oscillates robustly with an essentially wildtype period, indicating that the interaction between the positive and negative elements in the feedback loop is required for the operation of the circadian clock but is not a determinant of the period length.
    MeSH term(s) Circadian Clocks/genetics ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Neurospora crassa/drug effects ; Neurospora crassa/genetics ; Neurospora crassa/metabolism ; Transcription Factors/metabolism ; Protein Domains ; Gene Deletion ; Sodium Chloride/pharmacology ; Mutation ; Gene Expression
    Chemical Substances Fungal Proteins ; Transcription Factors ; FRQ protein, Neurospora crassa ; wc-1 protein, Neurospora crassa ; white collar 2 protein, Neurospora ; Sodium Chloride (451W47IQ8X)
    Language English
    Publishing date 2023-05-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1016/j.jbc.2023.104850
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.108: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Book: Homology effects

    Dunlap, Jay C.

    (Advances in genetics ; 46)

    2002  

    Author's details ed. by Jay C. Dunlap
    Series title Advances in genetics ; 46
    Collection
    Keywords Homologie ; Genetik
    Subject Allgemeine Genetik ; Erbbiologie ; Erbforschung ; Erblehre ; Vererbungslehre ; Vererbungswissenschaft ; Erblichkeitslehre
    Language English
    Size XXIII, 540 S. : Ill., graph. Darst.
    Publisher Acad. Press
    Publishing place San Diego u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT013316656
    ISBN 0-12-017646-7 ; 978-0-12-017646-5
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Se 836 -46- verfügbar in Königswinter Königswinter

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  7. Article ; Online: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability.

    Kelliher, Christina M / Stevenson, Elizabeth-Lauren / Loros, Jennifer J / Dunlap, Jay C

    PLoS biology

    2023  Volume 21, Issue 1, Page(s) e3001961

    Abstract: Compensation is a defining principle of a true circadian clock, where its approximately 24-hour period length is relatively unchanged across environmental conditions. Known compensation effectors directly regulate core clock factors to buffer the ... ...

    Abstract Compensation is a defining principle of a true circadian clock, where its approximately 24-hour period length is relatively unchanged across environmental conditions. Known compensation effectors directly regulate core clock factors to buffer the oscillator's period length from variables in the environment. Temperature Compensation mechanisms have been experimentally addressed across circadian model systems, but much less is known about the related process of Nutritional Compensation, where circadian period length is maintained across physiologically relevant nutrient levels. Using the filamentous fungus Neurospora crassa, we performed a genetic screen under glucose and amino acid starvation conditions to identify new regulators of Nutritional Compensation. Our screen uncovered 16 novel mutants, and together with 4 mutants characterized in prior work, a model emerges where Nutritional Compensation of the fungal clock is achieved at the levels of transcription, chromatin regulation, and mRNA stability. However, eukaryotic circadian Nutritional Compensation is completely unstudied outside of Neurospora. To test for conservation in cultured human cells, we selected top hits from our fungal genetic screen, performed siRNA knockdown experiments of the mammalian orthologs, and characterized the cell lines with respect to compensation. We find that the wild-type mammalian clock is also compensated across a large range of external glucose concentrations, as observed in Neurospora, and that knocking down the mammalian orthologs of the Neurospora compensation-associated genes CPSF6 or SETD2 in human cells also results in nutrient-dependent period length changes. We conclude that, like Temperature Compensation, Nutritional Compensation is a conserved circadian process in fungal and mammalian clocks and that it may share common molecular determinants.
    MeSH term(s) Humans ; Circadian Clocks/genetics ; Circadian Rhythm/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Glucose/metabolism ; Neurospora crassa/genetics ; Neurospora crassa/metabolism ; RNA Stability/genetics ; Nutrients/metabolism
    Chemical Substances Fungal Proteins ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2023-01-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3001961
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6193: Show issues Location:
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  8. Article ; Online: Phosphorylation, disorder, and phase separation govern the behavior of Frequency in the fungal circadian clock.

    Tariq, Daniyal / Maurici, Nicole / Bartholomai, Bradley M / Chandrasekaran, Siddarth / Dunlap, Jay C / Bah, Alaji / Crane, Brian R

    eLife

    2024  Volume 12

    Abstract: Circadian clocks are composed of transcription-translation negative feedback loops that pace rhythms of gene expression to the diurnal cycle. In the filamentous ... ...

    Abstract Circadian clocks are composed of transcription-translation negative feedback loops that pace rhythms of gene expression to the diurnal cycle. In the filamentous fungus
    MeSH term(s) Circadian Clocks/genetics ; Phosphorylation ; Phase Separation ; Fungal Proteins/metabolism ; Neurospora crassa/genetics ; Circadian Rhythm/genetics
    Chemical Substances Fungal Proteins
    Language English
    Publishing date 2024-03-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.90259
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Functional analysis of 110 phosphorylation sites on the circadian clock protein FRQ identifies clusters determining period length and temperature compensation.

    Wang, Bin / Stevenson, Elizabeth-Lauren / Dunlap, Jay C

    G3 (Bethesda, Md.)

    2022  Volume 13, Issue 2

    Abstract: ... periods were typically seen while removal of phosphorylation in the C-terminal tail resulted in extremely ... short periods, among the shortest reported. Interestingly, abolishing the 11 phosphosites in the C ...

    Abstract In the negative feedback loop driving the Neurospora circadian oscillator, the negative element, FREQUENCY (FRQ), inhibits its own expression by promoting phosphorylation of its heterodimeric transcriptional activators, White Collar-1 (WC-1) and WC-2. FRQ itself also undergoes extensive time-of-day-specific phosphorylation with over 100 phosphosites previously documented. Although disrupting individual or certain clusters of phosphorylation sites has been shown to alter circadian period lengths to some extent, it is still elusive how all the phosphorylations on FRQ control its activity. In this study, we systematically investigated the role in period determination of all 110 reported FRQ phosphorylation sites, using mutagenesis and luciferase reporter assays. Surprisingly, robust FRQ phosphorylation is still detected even when 84 phosphosites were eliminated altogether; further mutating another 26 phosphoresidues completely abolished FRQ phosphorylation. To identify phosphoresidue(s) on FRQ impacting circadian period length, a series of clustered frq phosphomutants covering all the 110 phosphosites were generated and examined for period changes. When phosphosites in the N-terminal and middle regions of FRQ were eliminated, longer periods were typically seen while removal of phosphorylation in the C-terminal tail resulted in extremely short periods, among the shortest reported. Interestingly, abolishing the 11 phosphosites in the C-terminal tail of FRQ not only results in an extremely short period, but also impacts temperature compensation (TC), yielding an overcompensated circadian oscillator. In addition, the few phosphosites in the middle of FRQ are also found to be crucial for TC. When different groups of FRQ phosphomutations were combined intramolecularly, expected additive effects were generally observed except for one novel case of intramolecular epistasis, where arrhythmicity resulting from one cluster of phosphorylation site mutants was restored by eliminating phosphorylation at another group of sites.
    MeSH term(s) Circadian Clocks/genetics ; Temperature ; Phosphorylation ; Circadian Rhythm/genetics ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Neurospora crassa/genetics ; Neurospora crassa/metabolism
    Chemical Substances Fungal Proteins
    Language English
    Publishing date 2022-12-19
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkac334
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Transcriptional rewiring of an evolutionarily conserved circadian clock.

    Goity, Alejandra / Dovzhenok, Andrey / Lim, Sookkyung / Hong, Christian / Loros, Jennifer / Dunlap, Jay C / Larrondo, Luis F

    The EMBO journal

    2024  

    Abstract: Circadian clocks temporally coordinate daily organismal biology over the 24-h cycle. Their molecular design, preserved between fungi and animals, is based on a core-oscillator composed of a one-step transcriptional-translational-negative-feedback-loop ( ... ...

    Abstract Circadian clocks temporally coordinate daily organismal biology over the 24-h cycle. Their molecular design, preserved between fungi and animals, is based on a core-oscillator composed of a one-step transcriptional-translational-negative-feedback-loop (TTFL). To test whether this evolutionarily conserved TTFL architecture is the only plausible way for achieving a functional circadian clock, we adopted a transcriptional rewiring approach, artificially co-opting regulators of the circadian output pathways into the core-oscillator. Herein we describe one of these semi-synthetic clocks which maintains all basic circadian features but, notably, it also exhibits new attributes such as a "lights-on timer" logic, where clock phase is fixed at the end of the night. Our findings indicate that fundamental circadian properties such as period, phase and temperature compensation are differentially regulated by transcriptional and posttranslational aspects of the clockworks.
    Language English
    Publishing date 2024-04-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.1038/s44318-024-00088-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MG 100: Show issues

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