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Article: Carotid Endarterectomy in the Very Elderly: Short-, Medium-, and Long-Term Outcomes.

Fahad, Shabin / Shirsath, Sayali / Metcalfe, Matthew / Elmallah, Ahmed

Vascular specialist international

2023 Volume 39, Page(s) 28

Abstract: Purpose: : Carotid endarterectomy (CEA) has an established effect on stroke-free survival in patients with carotid artery stenosis. Most landmark trials excluded patients ≥80 years of age due to their perceived high risk and uncertainty regarding the ... ...

Abstract	Purpose: : Carotid endarterectomy (CEA) has an established effect on stroke-free survival in patients with carotid artery stenosis. Most landmark trials excluded patients ≥80 years of age due to their perceived high risk and uncertainty regarding the benefits of CEA. Despite the ongoing global increase in life expectancy, guidelines have not changed. The current study aimed to assess CEA outcomes in patients ≥80 years of age. Materials and methods: : Data from patients ≥80 years of age, who underwent CEA between April 2016 and April 2022, were collected. Demographic information, comorbidities, surgical details, operative details, outcomes, and post-CEA survival were reviewed, and long-term data up to April 2023 were collected. Results: : Over the 6-year study period, 258 CEA procedures were recorded, of which 70 (27.1%) were performed in patients ≥80 years of age; the mean age was 84 years (range, 80-96 years), 47 (67.1%) were males, and 69 (98.6%) were symptomatic. Twenty-three (32.9%) patients were American Society of Anesthesiologists (ASA) grade 2, and 47 (67.1%) were grade 3. The 30-day stroke and mortality rates were 4.3% and 1.4%, respectively. At 1, 3, and 5 years, the cumulative freedom-from-stroke rates were 95.7%, 92.9%, and 91.4%, respectively, and the cumulative survival rates were 94.3%, 75.7%, and 61.4%, respectively. No risk factors affected early or late stroke or early mortality rates. Patients with ASA grade 3 had significantly lower cumulative survival than those with grade 2 (HR, 5.29; 95% CI, 1.590-17.603; P<0.01). Conclusion: : CEA was safe and effective in average-risk, elderly patients. Higher risk patients (i.e., ASA 3) showed no increased 30-day risk for stroke or mortality but exhibited significantly worse long-term survival. Hence, careful consideration of the benefits before performing CEA is crucial.
Language	English
Publishing date	2023-09-25
Publishing country	Korea (South)
Document type	Journal Article
ISSN	2288-7970
ISSN	2288-7970
DOI	10.5758/vsi.230060
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Article ; Online: Omission of a carbohydrate-rich breakfast impairs evening endurance exercise performance despite complete dietary compensation at lunch.

Metcalfe, Richard S / Thomas, Matthew / Lamb, Christopher / Chowdhury, Enhad A

European journal of sport science

2020 Volume 21, Issue 7, Page(s) 1013–1021

Abstract: Omission of a carbohydrate-rich breakfast followed by consuming ... ...

Abstract	Omission of a carbohydrate-rich breakfast followed by consuming an
MeSH term(s)	Adult ; Appetite ; Blood Glucose/metabolism ; Breakfast ; Dietary Carbohydrates/administration & dosage ; Energy Intake ; Heart Rate ; Humans ; Lactic Acid/blood ; Lunch ; Perception/physiology ; Physical Endurance/physiology ; Physical Exertion/physiology ; Time Factors ; Young Adult
Chemical Substances	Blood Glucose ; Dietary Carbohydrates ; Lactic Acid (33X04XA5AT)
Language	English
Publishing date	2020-08-27
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial
ISSN	1536-7290
ISSN (online)	1536-7290
DOI	10.1080/17461391.2020.1797890
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Article ; Online: Affecting Effects on Affect: The Impact of Protocol Permutations on Affective Responses to Sprint Interval Exercise; A Systematic Review and Meta-Analysis of Pooled Individual Participant Data.

Metcalfe, Richard S / Williams, Sean / Fernandes, Gwen S / Astorino, Todd A / Stork, Matthew J / Phillips, Shaun M / Niven, Ailsa / Vollaard, Niels B J

Frontiers in sports and active living

2022 Volume 4, Page(s) 815555

Abstract: ... modified by sprint duration: affect decreased more during 30 s (0.84 units/sprint; 95% CI: 0.74-0.93) and ... 15-20 s sprints (1.02 units/sprint; 95% CI: 0.93-1.10) compared with 5-6 s sprints (0.20 units/sprint ...

Abstract	Responses to sprint interval exercise (SIE) are hypothesized to be perceived as unpleasant, but SIE protocols are diverse, and moderating effects of various SIE protocol parameters on affective responses are unknown. We performed a systematic search to identify studies (up to 01/05/2021) measuring affective valence using the Feeling Scale during acute SIE in healthy adults. Thirteen studies involving 18 unique trials and 316 unique participant (142 women and 174 men) affective responses to SIE were eligible for inclusion. We received individual participant data for all participants from all studies. All available end-of-sprint affect scores from each trial were combined in a linear mixed model with sprint duration, mode, intensity, recovery duration, familiarization and baseline affect included as covariates. Affective valence decreased significantly and proportionally with each additional sprint repetition, but this effect was modified by sprint duration: affect decreased more during 30 s (0.84 units/sprint; 95% CI: 0.74-0.93) and 15-20 s sprints (1.02 units/sprint; 95% CI: 0.93-1.10) compared with 5-6 s sprints (0.20 units/sprint; 95% CI: 0.18-0.22) (both Systematic review registration: Open Science Framework, https://osf.io/sbyn3.
Language	English
Publishing date	2022-02-17
Publishing country	Switzerland
Document type	Systematic Review
ISSN	2624-9367
ISSN (online)	2624-9367
DOI	10.3389/fspor.2022.815555
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Article ; Online: Interleukin-34 permits Porphyromonas gingivalis survival and NF-κB p65 inhibition in macrophages.

Almarghlani, Ammar / Settem, Rajendra P / Croft, Andrew J / Metcalfe, Sarah / Giangreco, Matthew / Kay, Jason G

Molecular oral microbiology

2022 Volume 37, Issue 3, Page(s) 109–121

Abstract: Interleukin-34 (IL-34) is a cytokine that supports the viability and differentiation of macrophages. An important cytokine for the development of epidermal immunity, IL-34, is present and plays a role in the immunity of the oral environment. IL-34 has ... ...

Abstract	Interleukin-34 (IL-34) is a cytokine that supports the viability and differentiation of macrophages. An important cytokine for the development of epidermal immunity, IL-34, is present and plays a role in the immunity of the oral environment. IL-34 has been linked to inflammatory periodontal diseases, which involve innate phagocytes, including macrophages. Whether IL-34 can alter the ability of macrophages to effectively interact with oral microbes is currently unclear. Using macrophages derived from human blood monocytes with either the canonical cytokine colony-stimulating factor (CSF)1 or IL-34, we compared the ability of the macrophages to phagocytose, kill, and respond through the production of cytokines to the periodontal keystone pathogen Porphyromonas gingivalis. While macrophages derived from both cytokines were able to engulf the bacterium equally, IL-34-derived macrophages were much less capable of killing internalized P. gingivalis. Of the macrophage cell surface receptors known to interact with P. gingivalis, dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin was found to have the largest variation between IL-34- and CSF1-derived macrophages. We also found that upon interaction with P. gingivalis, IL-34-derived macrophages produced significantly less of the neutrophil chemotactic factor IL-8 than macrophages derived in the presence of CSF1. Mechanistically, we identified that the levels of IL-8 corresponded with P. gingivalis survival and dephosphorylation of the major transcription factor NF-κB p65. Overall, we found that macrophages differentiated in the presence of IL-34, a dominant cytokine in the oral gingiva, have a reduced ability to kill the keystone pathogen P. gingivalis and may be susceptible to specific bacteria-mediated cytokine modification.
MeSH term(s)	Bacteroidaceae Infections/immunology ; Gingiva/immunology ; Gingiva/microbiology ; Gingival Diseases/immunology ; Humans ; Interleukin-8 ; Interleukins/immunology ; Macrophages/immunology ; NF-kappa B/metabolism ; NF-kappa B/pharmacology ; Porphyromonas gingivalis/metabolism
Chemical Substances	IL34 protein, human ; Interleukin-8 ; Interleukins ; NF-kappa B
Language	English
Publishing date	2022-05-27
Publishing country	Denmark
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2537726-7
ISSN	2041-1014 ; 2041-1006
ISSN (online)	2041-1014
ISSN	2041-1006
DOI	10.1111/omi.12366
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Article ; Online: The REDUCED trial: a cluster randomized trial for REDucing the utilization of CEsarean delivery for dystocia.

Wood, Stephen / Skiffington, Janice / Brant, Rollin / Crawford, Susan / Hicks, Matthew / Mohammad, Khorshid / Mrklas, Kelly J / Tang, Selphee / Metcalfe, Amy

American journal of obstetrics and gynecology

2023 Volume 228, Issue 5S, Page(s) S1095–S1103

Abstract: Background: To reduce cesarean delivery rates in nulliparous women, guidelines for diagnosing nonprogressive labor have been developed by the National Institute of Child Health and Human Development, the American College of Obstetricians and ... ...

Abstract	Background: To reduce cesarean delivery rates in nulliparous women, guidelines for diagnosing nonprogressive labor have been developed by the National Institute of Child Health and Human Development, the American College of Obstetricians and Gynecologists, and the Society for Maternal-Fetal Medicine. These are mainly based on data from the Consortium for Safe Labor study. The guidelines have not been tested in a clinical trial, so the efficacy and safety of this new approach is uncertain. Objective: This study aimed to assess whether adoption of new guidelines for diagnosing nonprogressing labor would reduce cesarean delivery rates. Study design: We conducted a cluster randomized controlled trial of a knowledge translation program of the guidelines in 26 Canadian hospitals (13 control sites and 13 intervention sites). The sites included all intrapartum care sites in Alberta that perform cesarean delivery and deliver at least 70 nulliparous women annually. The baseline period started on January 1, 2015. The intervention was initiated at the first intervention site in January 2017. The follow-up period began at the first intervention site in February 2017 and lasted till February 2020. The primary outcome was the rate of cesarean delivery in nulliparous women with vertex presentation in labor at term. The secondary outcomes included spontaneous vaginal birth and maternal and neonatal safety. The main data source for the primary and secondary outcomes was the Alberta Perinatal Health Program database. The cesarean delivery rates were assessed using repeated measures mixed effects logistic regression applied to individual births. Results: The analysis was based on 45,193 deliveries at intervention sites and 43,725 deliveries at control sites. There was no evidence of a decrease in the rate of cesarean delivery in association with the intervention (baseline-adjusted odds ratio, 0.94; 95% confidence interval [0.85-1.05]; P=.259). The rate of spontaneous vaginal delivery increased slightly (baseline-adjusted odds ratio, 1.10; 95% confidence interval, [1.01-1.18]; P=.024). We did not observe any differences in adverse maternal or neonatal outcomes. Conclusion: Cesarean delivery rates in nulliparous women were not reduced by the application of recent guidelines for the diagnosis of nonprogressive labor. Spontaneous vaginal delivery-a secondary outcome-was increased in the intervention group. The intervention appears to be safe.
MeSH term(s)	Child ; Infant, Newborn ; Pregnancy ; Female ; Humans ; Canada ; Cesarean Section ; Delivery, Obstetric ; Labor, Obstetric ; Dystocia/epidemiology
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80016-8
ISSN	1097-6868 ; 0002-9378
ISSN (online)	1097-6868
ISSN	0002-9378
DOI	10.1016/j.ajog.2022.10.038
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Article ; Online: Prokaryotic microbial ecology as an ecosurveillance tool for eukaryotic pathogen colonisation: Meiothermus and Naegleria fowleri.

Malinowski, Natalia / Morgan, Matthew J / Wylie, Jason / Walsh, Tom / Domingos, Sergio / Metcalfe, Suzanne / Kaksonen, Anna H / Barnhart, Elliott P / Mueller, Rebecca / Peyton, Brent M / Puzon, Geoffrey J

Water research

2024 Volume 254, Page(s) 121426

Abstract: Naegleria fowleri has been detected in drinking water distribution systems (DWDS) in Australia, Pakistan and the United States and is the causative agent of the highly fatal disease primary amoebic meningoencephalitis. Previous small scale field studies ... ...

Abstract	Naegleria fowleri has been detected in drinking water distribution systems (DWDS) in Australia, Pakistan and the United States and is the causative agent of the highly fatal disease primary amoebic meningoencephalitis. Previous small scale field studies have shown that Meiothermus may be a potential biomarker for N. fowleri. However, correlations between predictive biomarkers in small sample sizes often breakdown when applied to larger more representative datasets. This study represents one of the largest and most rigorous temporal investigations of Naegleria fowleri colonisation in an operational DWDS in the world and measured the association of Meiothermus and N. fowleri over a significantly larger space and time in the DWDS. A total of 232 samples were collected from five sites over three-years (2016-2018), which contained 29 positive N. fowleri samples. Two specific operational taxonomic units assigned to M. chliarophilus and M. hypogaeus, were significantly associated with N. fowleri presence. Furthermore, inoculation experiments demonstrated that Meiothermus was required to support N. fowleri growth in field-collected biofilms. This validates Meiothermus as prospective biological tool to aid in the identification and surveillance of N. fowleri colonisable sites.
MeSH term(s)	Naegleria fowleri ; Prospective Studies ; Drinking Water ; Bacteria ; Biofilms
Chemical Substances	Drinking Water
Language	English
Publishing date	2024-03-05
Publishing country	England
Document type	Journal Article
ZDB-ID	202613-2
ISSN	1879-2448 ; 0043-1354
ISSN (online)	1879-2448
ISSN	0043-1354
DOI	10.1016/j.watres.2024.121426
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Article ; Online: Ultrasound appearances and histological correlation of native arteriovenous fistula stenoses - A retrospective case series.

Steiner, Kate / Ramanarayanan, Sivaramakrishnan / Metcalfe, Matthew / Jeevaratnum, Praveen / Selvakumar, Sadasivam / Narula, Ashish

Seminars in dialysis

2021 Volume 34, Issue 3, Page(s) 224–228

Abstract: The pathophysiology of haemodialysis arteriovenous fistulae (AVF) stenoses is not fully understood. The aim of this study was to perform histology assessment of stenoses in native AVF and compare and correlate the findings between ultrasound and ... ...

Abstract	The pathophysiology of haemodialysis arteriovenous fistulae (AVF) stenoses is not fully understood. The aim of this study was to perform histology assessment of stenoses in native AVF and compare and correlate the findings between ultrasound and histology. Intimal medial thickness (IMT) was measured on ultrasound where there was measurable neointimal hyperplasia at the site of stenosis and percentage intimal thickening calculated. Ultrasound findings were then compared with histology analysis of AVF stenoses in nine patients. In this small sample, different sonographic appearances and histology were demonstrated. Ultrasound demonstrated stenoses with neointimal hyperplasia and those with no measurable neointimal hyperplasia. Percentage intimal thickening was between 0% and 100%. The histology of the de novo stenoses (where no previous radiological or surgical procedure was performed) demonstrated stenoses with neointimal hyperplasia, no neointimal hyperplasia and neointimal hyperplasia and fibrosis. The histology findings after percutaneous angioplasty (PTA) demonstrated stenoses with neointimal hyperplasia and fibrosis and a stenosis with an acute inflammatory reaction. The findings in this small sample demonstrated that AVF stenoses are not a uniform group as demonstrated by different sonographic and corresponding appearances at histology. Ultrasound appearances of neointimal hyperplasia appear to correlate with neointimal hyperplasia on histology. These findings warrant further investigation and may have implications for treatment strategies.
MeSH term(s)	Arteriovenous Fistula ; Constriction, Pathologic ; Humans ; Neointima ; Renal Dialysis/adverse effects ; Retrospective Studies
Language	English
Publishing date	2021-01-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	1028193-9
ISSN	1525-139X ; 0894-0959
ISSN (online)	1525-139X
ISSN	0894-0959
DOI	10.1111/sdi.12947
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Article ; Online: Evaluation of process limit of detection and quantification variation of SARS-CoV-2 RT-qPCR and RT-dPCR assays for wastewater surveillance.

Ahmed, Warish / Bivins, Aaron / Metcalfe, Suzanne / Smith, Wendy J M / Verbyla, Matthew E / Symonds, Erin M / Simpson, Stuart L

Water research

2022 Volume 213, Page(s) 118132

Abstract: Effective wastewater surveillance of SARS-CoV-2 RNA requires the rigorous characterization of the limit of detection resulting from the entire sampling process - the process limit of detection (PLOD). Yet to date, no studies have gone beyond quantifying ... ...

Abstract	Effective wastewater surveillance of SARS-CoV-2 RNA requires the rigorous characterization of the limit of detection resulting from the entire sampling process - the process limit of detection (PLOD). Yet to date, no studies have gone beyond quantifying the assay limit of detection (ALOD) for RT-qPCR or RT-dPCR assays. While the ALOD is the lowest number of gene copies (GC) associated with a 95% probability of detection in a single PCR reaction, the PLOD represents the sensitivity of the method after considering the efficiency of all processing steps (e.g., sample handling, concentration, nucleic acid extraction, and PCR assays) to determine the number of GC in the wastewater sample matrix with a specific probability of detection. The primary objective of this study was to estimate the PLOD resulting from the combination of primary concentration and extraction with six SARS-CoV-2 assays: five RT-qPCR assays (US CDC N1 and N2, China CDC N and ORF1ab (CCDC N and CCDC ORF1ab), and E_Sarbeco RT-qPCR, and one RT-dPCR assay (US CDC N1 RT-dPCR) using two models (exponential survival and cumulative Gaussian). An adsorption extraction (AE) concentration method (i.e., virus adsorption on membrane and the RNA extraction from the membrane) was used to concentrate gamma-irradiated SARS-CoV-2 seeded into 36 wastewater samples. Overall, the US CDC N1 RT-dPCR and RT-qPCR assays had the lowest ALODs (< 10 GC/reaction) and PLODs (<3,954 GC/50 mL; 95% probability of detection) regardless of the seeding level and model used. Nevertheless, consistent amplification and detection rates decreased when seeding levels were < 2.32 × 10
Language	English
Publishing date	2022-02-03
Publishing country	England
Document type	Journal Article
ZDB-ID	202613-2
ISSN	1879-2448 ; 0043-1354
ISSN (online)	1879-2448
ISSN	0043-1354
DOI	10.1016/j.watres.2022.118132
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Article ; Online: Solving the conundrum of intra‐specific variation in metabolic rate: A multidisciplinary conceptual and methodological toolkit: New technical developments are opening the door to an understanding of why metabolic rate varies among individual animals of a species: New technical developments are opening the door to an understanding of why metabolic rate varies among individual animals of a species

Metcalfe, Neil B. / Bellman, Jakob / Bize, Pierre / Blier, Pierre U. / Crespel, Amélie / Dawson, Neal J. / Dunn, Ruth E. / Halsey, Lewis G. / Hood, Wendy R. / Hopkins, Mark / Killen, Shaun S. / McLennan, Darryl / Nadler, Lauren E. / Nati, Julie J.H. / Noakes, Matthew J. / Norin, Tommy / Ozanne, Susan E. / Peaker, Malcolm / Pettersen, Amanda K. /

Przybylska‐Piech, Anna / Rathery, Alann / Récapet, Charlotte / Rodriguez, Enrique / Salin, Karine / Stier, Antoine / Thoral, Elisa / Westerterp, Klaas R. / Westerterp‐Plantenga, Margriet S. / Wojciechowski, Michał S. / Monaghan, Patricia

BioEssays. 2023 June, v. 45, no. 6 p.e2300026-

2023

Abstract: Researchers from diverse disciplines, including organismal and cellular physiology, sports science, human nutrition, evolution and ecology, have sought to understand the causes and consequences of the surprising variation in metabolic rate found among ... ...

Abstract	Researchers from diverse disciplines, including organismal and cellular physiology, sports science, human nutrition, evolution and ecology, have sought to understand the causes and consequences of the surprising variation in metabolic rate found among and within individual animals of the same species. Research in this area has been hampered by differences in approach, terminology and methodology, and the context in which measurements are made. Recent advances provide important opportunities to identify and address the key questions in the field. By bringing together researchers from different areas of biology and biomedicine, we describe and evaluate these developments and the insights they could yield, highlighting the need for more standardisation across disciplines. We conclude with a list of important questions that can now be addressed by developing a common conceptual and methodological toolkit for studies on metabolic variation in animals.
Keywords	ecology ; evolution ; human nutrition ; medicine ; metabolism ; terminology
Language	English
Dates of publication	2023-06
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	50140-2
ISSN	1521-1878 ; 0265-9247
ISSN (online)	1521-1878
ISSN	0265-9247
DOI	10.1002/bies.202300026
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNA synthetase.

Xie, Stanley C / Wang, Yinuo / Morton, Craig J / Metcalfe, Riley D / Dogovski, Con / Pasaje, Charisse Flerida A / Dunn, Elyse / Luth, Madeline R / Kumpornsin, Krittikorn / Istvan, Eva S / Park, Joon Sung / Fairhurst, Kate J / Ketprasit, Nutpakal / Yeo, Tomas / Yildirim, Okan / Bhebhe, Mathamsanqa N / Klug, Dana M / Rutledge, Peter J / Godoy, Luiz C /

Dey, Sumanta / De Souza, Mariana Laureano / Siqueira-Neto, Jair L / Du, Yawei / Puhalovich, Tanya / Amini, Mona / Shami, Gerry / Loesbanluechai, Duangkamon / Nie, Shuai / Williamson, Nicholas / Jana, Gouranga P / Maity, Bikash C / Thomson, Patrick / Foley, Thomas / Tan, Derek S / Niles, Jacquin C / Han, Byung Woo / Goldberg, Daniel E / Burrows, Jeremy / Fidock, David A / Lee, Marcus C S / Winzeler, Elizabeth A / Griffin, Michael D W / Todd, Matthew H / Tilley, Leann

Nature communications

2024 Volume 15, Issue 1, Page(s) 937

Abstract: ... physical parameters and a synthetically accessible scaffold. We show that the exemplar, OSM-S-106, has ... that OSM-S-106 inhibits protein translation and activates the amino acid starvation response. Targeted ... mass spectrometry confirms that OSM-S-106 is a pro-inhibitor and that inhibition of PfAsnRS occurs via enzyme ...

Abstract	Malaria poses an enormous threat to human health. With ever increasing resistance to currently deployed drugs, breakthrough compounds with novel mechanisms of action are urgently needed. Here, we explore pyrimidine-based sulfonamides as a new low molecular weight inhibitor class with drug-like physical parameters and a synthetically accessible scaffold. We show that the exemplar, OSM-S-106, has potent activity against parasite cultures, low mammalian cell toxicity and low propensity for resistance development. In vitro evolution of resistance using a slow ramp-up approach pointed to the Plasmodium falciparum cytoplasmic asparaginyl-tRNA synthetase (PfAsnRS) as the target, consistent with our finding that OSM-S-106 inhibits protein translation and activates the amino acid starvation response. Targeted mass spectrometry confirms that OSM-S-106 is a pro-inhibitor and that inhibition of PfAsnRS occurs via enzyme-mediated production of an Asn-OSM-S-106 adduct. Human AsnRS is much less susceptible to this reaction hijacking mechanism. X-ray crystallographic studies of human AsnRS in complex with inhibitor adducts and docking of pro-inhibitors into a model of Asn-tRNA-bound PfAsnRS provide insights into the structure-activity relationship and the selectivity mechanism.
MeSH term(s)	Animals ; Humans ; Plasmodium falciparum/genetics ; Asparagine/metabolism ; Aspartate-tRNA Ligase/genetics ; RNA, Transfer, Amino Acyl/metabolism ; Antimalarials/pharmacology ; Mammals/genetics
Chemical Substances	asparaginyl-tRNA synthetase (EC 6.1.1.22) ; Asparagine (7006-34-0) ; Aspartate-tRNA Ligase (EC 6.1.1.12) ; RNA, Transfer, Amino Acyl ; Antimalarials
Language	English
Publishing date	2024-01-31
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45224-z
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