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Article ; Online: Complete Genome Sequence of Legionella cardiaca Strain H63

Lopez, Alberto E / Mayoral, Joshua / Cianciotto, Nicholas P

Microbiology resource announcements

2023 Volume 12, Issue 7, Page(s) e0017523

Abstract: We report the complete genome sequence of Legionella cardiaca strain ... ...

Abstract	We report the complete genome sequence of Legionella cardiaca strain H63
Language	English
Publishing date	2023-06-13
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.00175-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection.

Campbell, Jackson A / Cianciotto, Nicholas P

Infection and immunity

2022 Volume 90, Issue 10, Page(s) e0036922

Abstract: Previously, we demonstrated that Cas2 encoded within the CRISPR-Cas locus of Legionella pneumophila strain 130b promotes the ability of ... ...

Abstract	Previously, we demonstrated that Cas2 encoded within the CRISPR-Cas locus of Legionella pneumophila strain 130b promotes the ability of the
MeSH term(s)	Humans ; Legionella pneumophila/physiology ; Heat-Shock Proteins, Small/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Acanthamoeba castellanii ; Ribonucleases/metabolism
Chemical Substances	Heat-Shock Proteins, Small ; Bacterial Proteins ; Ribonucleases (EC 3.1.-)
Language	English
Publishing date	2022-09-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	218698-6
ISSN	1098-5522 ; 0019-9567
ISSN (online)	1098-5522
ISSN	0019-9567
DOI	10.1128/iai.00369-22
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Book ; Conference proceedings: Legionella

Cianciotto, Nicholas P.

state of the art 30 years after its recognition ; [... researchers from around the world gathered in Chicago, Illinois, USA on October 16 - 20, 2005 for the 6th International Conference on Legionella]

2006

Event/congress	International Conference on Legionella (6, 2005, ChicagoIll.)
Author's details	ed. by Nicholas P. Cianciotto
Keywords	Legionellosis ; Legionella ; Legionnaires Disease
Language	English
Size	XXI, 565 S. : Ill., graph. Darst.
Publisher	ASM Press
Publishing place	Washington, DC
Publishing country	United States
Document type	Book ; Conference proceedings
HBZ-ID	HT014833098
ISBN	1-55581-390-9 ; 978-1-55581-390-1
Database	Catalogue ZB MED Medicine, Health

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2007 A 946	order for loan	Magazin

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Article ; Online: Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensor.

Grigoryeva, Lubov S / Cianciotto, Nicholas P

PLoS pathogens

2021 Volume 17, Issue 7, Page(s) e1009781

Abstract: Cytokines made by macrophages play a critical role in determining the course of Legionella pneumophila infection. Prior murine-based modeling indicated that this cytokine response is initiated upon recognition of L. pneumophila by a subset of Toll-like ... ...

Abstract	Cytokines made by macrophages play a critical role in determining the course of Legionella pneumophila infection. Prior murine-based modeling indicated that this cytokine response is initiated upon recognition of L. pneumophila by a subset of Toll-like receptors, namely TLR2, TLR5, and TLR9. Through the use of shRNA/siRNA knockdowns and subsequently CRISPR/Cas9 knockouts (KO), we determined that TRIF, an adaptor downstream of endosomal TLR3 and TLR4, is required for full cytokine secretion by human primary and cell-line macrophages. By characterizing a further set of TLR KO's in human U937 cells, we discerned that, contrary to the viewpoint garnered from murine-based studies, TLR3 and TLR4 (along with TLR2 and TLR5) are in fact vital to the macrophage response in the early stages of L. pneumophila infection. This conclusion was bolstered by showing that i) chemical inhibitors of TLR3 and TLR4 dampen the cytokine output of primary human macrophages and ii) transfection of TLR3 and TLR4 into HEK cells conferred an ability to sense L. pneumophila. TLR3- and TLR4-dependent cytokines promoted migration of human HL-60 neutrophils across an epithelial layer, pointing to the biological importance for the newfound signaling pathway. The response of U937 cells to L. pneumophila LPS was dependent upon TLR4, a further contradiction to murine-based studies, which had concluded that TLR2 is the receptor for Legionella LPS. Given the role of TLR3 in sensing nucleic acid (i.e., dsRNA), we utilized newly-made KO U937 cells to document that DNA-sensing by cGAS-STING and DNA-PK are also needed for the response of human macrophages to L. pneumophila. Given the lack of attention given them in the bacterial field, C-type lectin receptors were similarly examined; but, they were not required. Overall, this study arguably represents the most extensive, single-characterization of Legionella-recognition receptors within human macrophages.
MeSH term(s)	Animals ; Bacterial Proteins/immunology ; Humans ; Legionella pneumophila/immunology ; Legionnaires' Disease/immunology ; Lipopolysaccharides/immunology ; Macrophages/immunology ; Macrophages/metabolism ; Mice ; Pathogen-Associated Molecular Pattern Molecules/immunology ; Pathogen-Associated Molecular Pattern Molecules/metabolism ; Toll-Like Receptor 3/immunology ; Toll-Like Receptor 3/metabolism ; Toll-Like Receptor 4/immunology ; Toll-Like Receptor 4/metabolism
Chemical Substances	Bacterial Proteins ; Lipopolysaccharides ; Pathogen-Associated Molecular Pattern Molecules ; Toll-Like Receptor 3 ; Toll-Like Receptor 4
Language	English
Publishing date	2021-07-19
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1009781
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Legionella pneumophila Rhizoferrin Promotes Bacterial Biofilm Formation and Growth within Amoebae and Macrophages.

Lopez, Alberto E / Grigoryeva, Lubov S / Barajas, Armando / Cianciotto, Nicholas P

Infection and immunity

2023 Volume 91, Issue 8, Page(s) e0007223

Abstract: Previously, we showed that Legionella pneumophila secretes rhizoferrin, a polycarboxylate siderophore that promotes bacterial growth in iron-deplete media and the murine lung. Yet, past studies failed to identify a role for the rhizoferrin biosynthetic ... ...

Abstract	Previously, we showed that Legionella pneumophila secretes rhizoferrin, a polycarboxylate siderophore that promotes bacterial growth in iron-deplete media and the murine lung. Yet, past studies failed to identify a role for the rhizoferrin biosynthetic gene (
MeSH term(s)	Animals ; Mice ; Humans ; Legionella pneumophila/genetics ; Legionella pneumophila/metabolism ; Siderophores/metabolism ; Amoeba/metabolism ; U937 Cells ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Iron/metabolism ; Macrophages/microbiology ; Biofilms
Chemical Substances	rhizoferrin (138846-62-5) ; Siderophores ; Bacterial Proteins ; Iron (E1UOL152H7)
Language	English
Publishing date	2023-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	218698-6
ISSN	1098-5522 ; 0019-9567
ISSN (online)	1098-5522
ISSN	0019-9567
DOI	10.1128/iai.00072-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Effectors of the Stenotrophomonas maltophilia Type IV Secretion System Mediate Killing of Clinical Isolates of Pseudomonas aeruginosa.

Nas, Megan Y / Gabell, Jeffrey / Cianciotto, Nicholas P

mBio

2021 Volume 12, Issue 3, Page(s) e0150221

Abstract: ... maltophilia. The immunity proteins of two of these proved especially capable of protecting P. aeruginosa and ...

Abstract	Previously, we documented that Stenotrophomonas maltophilia encodes a type IV secretion system (T4SS) that allows the organism to kill, in contact-dependent fashion, heterologous bacteria, including wild-type Pseudomonas aeruginosa. Bioinformatic screens based largely on the presence of both a C-terminal consensus sequence and an adjacent gene encoding a cognate immunity protein identified 13 potential antibacterial effectors, most of which were highly conserved among sequenced strains of S. maltophilia. The immunity proteins of two of these proved especially capable of protecting P. aeruginosa and Escherichia coli against attack from the
MeSH term(s)	Antibiosis/genetics ; Escherichia coli/metabolism ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/prevention & control ; Humans ; Pseudomonas aeruginosa/metabolism ; Stenotrophomonas maltophilia/genetics ; Stenotrophomonas maltophilia/metabolism ; Type IV Secretion Systems/genetics ; Type IV Secretion Systems/metabolism
Chemical Substances	Type IV Secretion Systems
Language	English
Publishing date	2021-06-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.01502-21
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Article ; Online: Analysis of Iron Requirements and Siderophore Production.

Burnside, Denise M / Cianciotto, Nicholas P

Methods in molecular biology (Clifton, N.J.)

2019 Volume 1921, Page(s) 3–19

Abstract: This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This ... ...

Abstract	This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This chapter then describes how to purify siderophore from culture supernatants through sequential reversed-phase/weak-anion exchange chromatography and high-performance liquid chromatography. The next section describes assays which allow the detection of the iron-binding capability and the biological activity of the purified siderophore. Lastly, this chapter describes the growth of L. pneumophila in chemically defined liquid medium (CDM) containing various iron sources as a method to assess the iron requirements of L. pneumophila.
MeSH term(s)	Biological Assay ; Chromatography, High Pressure Liquid ; Culture Media, Conditioned/chemistry ; Culture Media, Conditioned/metabolism ; Iron/chemistry ; Iron/metabolism ; Legionella pneumophila/metabolism ; Metabolic Networks and Pathways ; Molecular Structure ; Siderophores/biosynthesis ; Siderophores/chemistry ; Siderophores/isolation & purification
Chemical Substances	Culture Media, Conditioned ; Siderophores ; Iron (E1UOL152H7)
Language	English
Publishing date	2019-02-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-9048-1_1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.

White, Richard C / Cianciotto, Nicholas P

Microbial genomics

2019 Volume 5, Issue 6

Abstract: The type II secretion system (T2SS) plays a major role in promoting bacterial survival in the environment and in human hosts. One of the best characterized T2SS is that of Legionella pneumophila, the agent of Legionnaires' disease. Secreting at least 25 ... ...

Abstract	The type II secretion system (T2SS) plays a major role in promoting bacterial survival in the environment and in human hosts. One of the best characterized T2SS is that of Legionella pneumophila, the agent of Legionnaires' disease. Secreting at least 25 proteins, including degradative enzymes, eukaryotic-like proteins and novel effectors, this T2SS contributes to the ability of L. pneumophila to grow at low temperatures, infect amoebal and macrophage hosts, damage lung tissue, evade the immune system, and undergo sliding motility. The genes encoding the T2SS are conserved across the genus Legionella, which includes 62 species and >30 pathogens in addition to L. pneumophila. The vast majority of effectors associated with L. pneumophila are shared by a large number of Legionella species, hinting at a critical role for them in the ecology of Legionella as a whole. However, no other species has the same repertoire as L. pneumophila, with, as a general rule, phylogenetically more closely related species sharing similar sets of effectors. T2SS effectors that are involved in infection of a eukaryotic host(s) are more prevalent throughout Legionella, indicating that they are under stronger selective pressure. The Legionella T2SS apparatus is closest to that of Aquicella (another parasite of amoebae), and a significant number of L. pneumophila effectors have their closest homologues in Aquicella. Thus, the T2SS of L. pneumophila probably originated within the order Legionellales, with some of its effectors having arisen within that Aquicella-like progenitor, while other effectors derived from the amoebal host, mimiviruses, fungi and less closely related bacteria.
MeSH term(s)	Bacterial Proteins/genetics ; Genome, Bacterial/genetics ; Genomics/methods ; Humans ; Legionella/genetics ; Legionella/metabolism ; Legionella pneumophila/genetics ; Legionella pneumophila/metabolism ; Legionnaires' Disease/metabolism ; Phylogeny ; Type II Secretion Systems/genetics ; Type II Secretion Systems/metabolism ; Type II Secretion Systems/physiology ; Virulence Factors/genetics
Chemical Substances	Bacterial Proteins ; Type II Secretion Systems ; Virulence Factors
Language	English
Publishing date	2019-06-05
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2835258-0
ISSN	2057-5858 ; 2057-5858
ISSN (online)	2057-5858
ISSN	2057-5858
DOI	10.1099/mgen.0.000273
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Article ; Online: An update on iron acquisition by Legionella pneumophila: new pathways for siderophore uptake and ferric iron reduction.

Cianciotto, Nicholas P

Future microbiology

2015 Volume 10, Issue 5, Page(s) 841–851

Abstract: Iron acquisition is critical for the growth and pathogenesis of Legionella pneumophila, the causative agent of Legionnaires' disease. L. pneumophila utilizes two main modes of iron assimilation, namely ferrous iron uptake via the FeoB system and ferric ... ...

Abstract	Iron acquisition is critical for the growth and pathogenesis of Legionella pneumophila, the causative agent of Legionnaires' disease. L. pneumophila utilizes two main modes of iron assimilation, namely ferrous iron uptake via the FeoB system and ferric iron acquisition through the action of the siderophore legiobactin. This review highlights recent studies concerning the mechanism of legiobactin assimilation, the impact of c-type cytochromes on siderophore production, the importance of legiobactin in lung infection and a newfound role for a bacterial pyomelanin in iron acquisition. These data demonstrate that key aspects of L. pneumophila iron acquisition are significantly distinct from those of long-studied, 'model' organisms. Indeed, L. pneumophila may represent a new paradigm for a variety of other intracellular parasites, pathogens and under-studied bacteria.
MeSH term(s)	Bacterial Proteins/metabolism ; Cytochromes c/metabolism ; Ferric Compounds/metabolism ; Ferrous Compounds/metabolism ; Humans ; Iron/metabolism ; Legionella pneumophila/metabolism ; Legionellosis/microbiology ; Legionellosis/pathology ; Melanins/metabolism ; Metabolic Networks and Pathways ; Oxidation-Reduction ; Siderophores/metabolism
Chemical Substances	Bacterial Proteins ; Ferric Compounds ; Ferrous Compounds ; Melanins ; Siderophores ; legiobactin protein, Legionella pneumophila ; pyomelanin ; Cytochromes c (9007-43-6) ; Iron (E1UOL152H7)
Language	English
Publishing date	2015
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ISSN	1746-0921
ISSN (online)	1746-0921
DOI	10.2217/fmb.15.21
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensor.

Lubov S Grigoryeva / Nicholas P Cianciotto

PLoS Pathogens, Vol 17, Iss 7, p e

2021 Volume 1009781

Abstract	Cytokines made by macrophages play a critical role in determining the course of Legionella pneumophila infection. Prior murine-based modeling indicated that this cytokine response is initiated upon recognition of L. pneumophila by a subset of Toll-like receptors, namely TLR2, TLR5, and TLR9. Through the use of shRNA/siRNA knockdowns and subsequently CRISPR/Cas9 knockouts (KO), we determined that TRIF, an adaptor downstream of endosomal TLR3 and TLR4, is required for full cytokine secretion by human primary and cell-line macrophages. By characterizing a further set of TLR KO's in human U937 cells, we discerned that, contrary to the viewpoint garnered from murine-based studies, TLR3 and TLR4 (along with TLR2 and TLR5) are in fact vital to the macrophage response in the early stages of L. pneumophila infection. This conclusion was bolstered by showing that i) chemical inhibitors of TLR3 and TLR4 dampen the cytokine output of primary human macrophages and ii) transfection of TLR3 and TLR4 into HEK cells conferred an ability to sense L. pneumophila. TLR3- and TLR4-dependent cytokines promoted migration of human HL-60 neutrophils across an epithelial layer, pointing to the biological importance for the newfound signaling pathway. The response of U937 cells to L. pneumophila LPS was dependent upon TLR4, a further contradiction to murine-based studies, which had concluded that TLR2 is the receptor for Legionella LPS. Given the role of TLR3 in sensing nucleic acid (i.e., dsRNA), we utilized newly-made KO U937 cells to document that DNA-sensing by cGAS-STING and DNA-PK are also needed for the response of human macrophages to L. pneumophila. Given the lack of attention given them in the bacterial field, C-type lectin receptors were similarly examined; but, they were not required. Overall, this study arguably represents the most extensive, single-characterization of Legionella-recognition receptors within human macrophages.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
Subject code	572 ; 610
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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