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Book: Krebsrisiko durch beruflichen Umgang mit Zytostatika

Roller, Markus / Eickmann, Udo / Nies, Eberhard

quantitative Betrachtungen

(BIA-Report ; 2001,5)

2001

Author's details	[Verf.: Markus Roller ; Udo Eickmann ; Eberhard Nies]
Series title	BIA-Report ; 2001,5
Collection
Keywords	Cytostatikum ; Carcinogenität
Subject	Karzinogenität ; Cancerogenität ; Kanzerogenität ; Zytostatikum ; Cancerotoxischer Stoff ; Carcinostatikum ; Krebsmittel ; Antineoplastikum ; Anticarcinogen ; Antikarzinogen ; Anticancerogen ; Antineoplastisches Mittel
Language	German
Size	157 S. : graph. Darst.
Publisher	HVBG, Hauptverband der Gewerbl. Berufsgenossenschaften
Publishing place	Sankt Augustin
Publishing country	Germany
Document type	Book
Note	Zsfassung in dt., engl., franz. und span. Sprache
HBZ-ID	HT013232377
ISBN	3-88383-581-1 ; 978-3-88383-581-5
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

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2002 A 5879	order for loan	Magazin

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Article: Rescue and characterization of the first West African Marburg virus 2021 from Guinea.

von Creytz, Isabel / Gerresheim, Gesche K / Lier, Clemens / Schneider, Jana / Schauflinger, Martin / Benz, Marcel / Kämper, Lennart / Rohde, Cornelius / Eickmann, Markus / Biedenkopf, Nadine

Heliyon

2023 Volume 9, Issue 9, Page(s) e19613

Abstract: Marburg virus (MARV) is a causative agent of a severe hemorrhagic fever with high fatality rates endemic in central Africa. Current outbreaks of MARV in Equatorial Guinea and Tanzania underline the relevance of MARV as a public health emergency pathogen. ...

Abstract	Marburg virus (MARV) is a causative agent of a severe hemorrhagic fever with high fatality rates endemic in central Africa. Current outbreaks of MARV in Equatorial Guinea and Tanzania underline the relevance of MARV as a public health emergency pathogen. In 2021, the first known human MARV case was confirmed in Guinea, West Africa. Since no infectious virus could be isolated from that fatal case in 2021, we generated recombinant (rec) MARV Guinea by reverse genetics in order to study and characterize this new MARV, which occurred in West Africa for the first time, in terms of its growth properties, detection by antibodies, and therapeutic potential compared to known MARV strains. Our results showed a solid viral replication of recMARV Guinea in human, bat, and monkey cell lines in comparison to other known MARV strains. We further demonstrated that replication of recMARV Guinea in cells can be inhibited by the nucleoside analogue remdesivir. Taken together, we could successfully reconstitute
Language	English
Publishing date	2023-08-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e19613
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: In Vitro Bactericidal and Virucidal Efficacy of Povidone-Iodine Gargle/Mouthwash Against Respiratory and Oral Tract Pathogens.

Eggers, Maren / Koburger-Janssen, Torsten / Eickmann, Markus / Zorn, Juergen

Infectious diseases and therapy

2018 Volume 7, Issue 2, Page(s) 249–259

Abstract: Introduction: Recent virus epidemics and rising antibiotic resistance highlight the importance of hygiene measures to prevent and control outbreaks. We investigated the in vitro bactericidal and virucidal efficacy of povidone-iodine (PVP-I) 7% gargle/ ... ...

Abstract	Introduction: Recent virus epidemics and rising antibiotic resistance highlight the importance of hygiene measures to prevent and control outbreaks. We investigated the in vitro bactericidal and virucidal efficacy of povidone-iodine (PVP-I) 7% gargle/mouthwash at defined dilution against oral and respiratory tract pathogens. Methods: PVP-I was tested against Klebsiella pneumoniae and Streptococcus pneumoniae according to bactericidal quantitative suspension test EN13727 and against severe acute respiratory syndrome and Middle East respiratory syndrome coronaviruses (SARS-CoV and MERS-CoV), rotavirus strain Wa and influenza virus A subtype H1N1 according to virucidal quantitative suspension test EN14476. PVP-I 7% gargle/mouthwash was diluted 1:30 with water to a concentration of 0.23% (the recommended concentration for "real-life" use in Japan) and tested at room temperature under clean conditions [0.3 g/l bovine serum albumin (BSA), viruses only] and dirty conditions (3.0 g/l BSA + 3.0 ml/l erythrocytes) as an interfering substance for defined contact times (minimum 15 s). Rotavirus was tested without protein load. A ≥ 5 log Results: PVP-I gargle/mouthwash diluted 1:30 (equivalent to a concentration of 0.23% PVP-I) showed effective bactericidal activity against Klebsiella pneumoniae and Streptococcus pneumoniae and rapidly inactivated SARS-CoV, MERS-CoV, influenza virus A (H1N1) and rotavirus after 15 s of exposure. Conclusion: PVP-I 7% gargle/mouthwash showed rapid bactericidal activity and virucidal efficacy in vitro at a concentration of 0.23% PVP-I and may provide a protective oropharyngeal hygiene measure for individuals at high risk of exposure to oral and respiratory pathogens. Funding: Mundipharma Research GmbH & Co. KG (MRG).
Keywords	covid19
Language	English
Publishing date	2018-04-09
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2701611-0
ISSN	2193-6382 ; 2193-8229
ISSN (online)	2193-6382
ISSN	2193-8229
DOI	10.1007/s40121-018-0200-7
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Article ; Online: Rescue and characterization of the first West African Marburg virus 2021 from Guinea

Isabel von Creytz / Gesche K. Gerresheim / Clemens Lier / Jana Schneider / Martin Schauflinger / Marcel Benz / Lennart Kämper / Cornelius Rohde / Markus Eickmann / Nadine Biedenkopf

Heliyon, Vol 9, Iss 9, Pp e19613- (2023)

2023

Abstract	Marburg virus (MARV) is a causative agent of a severe hemorrhagic fever with high fatality rates endemic in central Africa. Current outbreaks of MARV in Equatorial Guinea and Tanzania underline the relevance of MARV as a public health emergency pathogen. In 2021, the first known human MARV case was confirmed in Guinea, West Africa. Since no infectious virus could be isolated from that fatal case in 2021, we generated recombinant (rec) MARV Guinea by reverse genetics in order to study and characterize this new MARV, which occurred in West Africa for the first time, in terms of its growth properties, detection by antibodies, and therapeutic potential compared to known MARV strains. Our results showed a solid viral replication of recMARV Guinea in human, bat, and monkey cell lines in comparison to other known MARV strains. We further demonstrated that replication of recMARV Guinea in cells can be inhibited by the nucleoside analogue remdesivir. Taken together, we could successfully reconstitute de novo the first West African MARV from Guinea showing similar replication kinetics in cells compared to other central African MARV strains. Our reverse genetics approach has proven successful in characterizing emerging viruses, especially when virus isolates are missing and viral genome sequences are incomplete.
Keywords	Marburg virus ; Filovirus ; Reverse genetics ; Full-length clone ; Virus replication ; Remdesivir ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	570
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: Intranasal Borna Disease Virus (BoDV-1) Infection: Insights into Initial Steps and Potential Contagiosity.

Kupke, Alexandra / Becker, Sabrina / Wewetzer, Konstantin / Ahlemeyer, Barbara / Eickmann, Markus / Herden, Christiane

International journal of molecular sciences

2019 Volume 20, Issue 6

Abstract: Mammalian Bornavirus (BoDV-1) typically causes a fatal neurologic disorder in horses and sheep, and was recently shown to cause fatal encephalitis in humans with and without transplant reception. It has been suggested that BoDV-1 enters the central ... ...

Abstract	Mammalian Bornavirus (BoDV-1) typically causes a fatal neurologic disorder in horses and sheep, and was recently shown to cause fatal encephalitis in humans with and without transplant reception. It has been suggested that BoDV-1 enters the central nervous system (CNS) via the olfactory pathway. However, (I) susceptible cell types that replicate the virus for successful spread, and (II) the role of olfactory ensheathing cells (OECs), remained unclear. To address this, we studied the intranasal infection of adult rats with BoDV-1 in vivo and in vitro, using olfactory mucosal (OM) cell cultures and the cultures of purified OECs. Strikingly, in vitro and in vivo, viral antigen and mRNA were present from four days post infection (dpi) onwards in the olfactory receptor neurons (ORNs), but also in all other cell types of the OM, and constantly in the OECs. In contrast, in vivo, BoDV-1 genomic RNA was only detectable in adult and juvenile ORNs, nerve fibers, and in OECs from 7 dpi on. In vitro, the rate of infection of OECs was significantly higher than that of the OM cells, pointing to a crucial role of OECs for infection via the olfactory pathway. Thus, this study provides important insights into the transmission of neurotropic viral infections with a zoonotic potential.
MeSH term(s)	Animals ; Borna Disease/virology ; Borna disease virus/genetics ; Borna disease virus/pathogenicity ; Cell Culture Techniques ; Cells, Cultured ; Disease Models, Animal ; Humans ; Olfactory Bulb/cytology ; Olfactory Bulb/virology ; Olfactory Mucosa/cytology ; Olfactory Mucosa/virology ; RNA, Viral/genetics ; Rats ; Zoonoses/virology
Chemical Substances	RNA, Viral
Language	English
Publishing date	2019-03-15
Publishing country	Switzerland
Document type	Comparative Study ; Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20061318
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Article: Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acids Potently Reduce Ebola Virus Infection In Vitro.

Sadewasser, Anne / Dietzel, Erik / Michel, Sven / Klüver, Michael / Helfer, Markus / Thelemann, Tamara / Klar, Richard / Eickmann, Markus / Becker, Stephan / Jaschinski, Frank

Molecular therapy. Nucleic acids

2019 Volume 16, Page(s) 686–697

Abstract: Ebola virus is the causative agent of Ebola virus disease, a severe, often fatal illness in humans. So far, there are no US Food and Drug Administration (FDA)-approved therapeutics directed against Ebola virus. Here, we selected the host factor Niemann- ... ...

Abstract	Ebola virus is the causative agent of Ebola virus disease, a severe, often fatal illness in humans. So far, there are no US Food and Drug Administration (FDA)-approved therapeutics directed against Ebola virus. Here, we selected the host factor Niemann-Pick C1 (NPC1), which has been shown to be essential for Ebola virus entry into host cytoplasm, as a therapeutic target for suppression by locked nucleic acid-modified antisense oligonucleotides. Screening of antisense oligonucleotides in human and murine cell lines led to identification of candidates with up to 94% knockdown efficiency and 50% inhibitory concentration (IC
Language	English
Publishing date	2019-04-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	2662631-7
ISSN	2162-2531
ISSN	2162-2531
DOI	10.1016/j.omtn.2019.04.018
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Article ; Online: Optimization of RNA extraction protocol for long-term archived formalin-fixed paraffin-embedded tissues of horses.

Boos, Gisele Silva / Nobach, Daniel / Failing, Klaus / Eickmann, Markus / Herden, Christiane

Experimental and molecular pathology

2019 Volume 110, Page(s) 104289

Abstract: A suitable RNA extraction protocol was established to gain high quality RNA from formalin-fixed paraffin-embedded tissues to perform reliable molecular assays either applicable for using FFPE tissue archives or tissues with harsh formalin-fixation. ... ...

Abstract	A suitable RNA extraction protocol was established to gain high quality RNA from formalin-fixed paraffin-embedded tissues to perform reliable molecular assays either applicable for using FFPE tissue archives or tissues with harsh formalin-fixation. Eighteen FFPE samples from the central nervous system of horses, stored up to 11 years, were used as archive cases. To test the influence of the fixation period, brain, liver, kidney, and skeletal muscle tissue fragments from another horse, were treated either with water or tris-acetate-EDTA buffer after fixation under different timepoints with 10% unbuffered formalin. Two deparaffinization methods and three proteinase K-based lysis step were tested and translated into three protocols. After detailed statistical analysis it was determined that a longer period and increase in volume of proteinase K incubation provide higher yields and purity of RNA (P < 0.01) of archived samples. Alongside, amplification of equid-housekeeping gene up to 298 bp was successful with the protocol adaptations. For different formalin-fixation timepoints, it was demonstrated that the right choice for treatment and formalin-fixation period is organ-related (P ≤ 0.05). Essentially, little alterations to pre-existing extraction protocols unwound the RNA of up to 11-year-old samples, enabling the use of FFPE tissue archives or e.g. harshly fixed material needed in infection research under high biosafety levels for a variety of molecular analysis.
MeSH term(s)	Animals ; Formaldehyde/chemistry ; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/metabolism ; Horses ; Paraffin Embedding/methods ; Paraffin Embedding/veterinary ; RNA/analysis ; RNA/genetics ; RNA/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/veterinary ; Specimen Handling/standards ; Tissue Fixation/methods ; Tissue Fixation/veterinary
Chemical Substances	Formaldehyde (1HG84L3525) ; RNA (63231-63-0) ; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) (EC 1.2.1.9)
Language	English
Publishing date	2019-07-23
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	207655-x
ISSN	1096-0945 ; 0014-4800
ISSN (online)	1096-0945
ISSN	0014-4800
DOI	10.1016/j.yexmp.2019.104289
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Article: Protective CD8+ T Cell Response Induced by Modified Vaccinia Virus Ankara Delivering Ebola Virus Nucleoprotein.

Kupke, Alexandra / Volz, Asisa / Dietzel, Erik / Freudenstein, Astrid / Schmidt, Jörg / Shams-Eldin, Hosam / Jany, Sylvia / Sauerhering, Lucie / Krähling, Verena / Gellhorn Serra, Michelle / Herden, Christiane / Eickmann, Markus / Becker, Stephan / Sutter, Gerd

Vaccines

2022 Volume 10, Issue 4

Abstract: The urgent need for vaccines against Ebola virus (EBOV) was underscored by the large outbreak in West Africa (2014-2016). Since then, several promising vaccine candidates have been tested in pre-clinical and clinical studies. As a result, two vaccines ... ...

Abstract	The urgent need for vaccines against Ebola virus (EBOV) was underscored by the large outbreak in West Africa (2014-2016). Since then, several promising vaccine candidates have been tested in pre-clinical and clinical studies. As a result, two vaccines were approved for human use in 2019/2020, of which one includes a heterologous adenovirus/Modified Vaccinia virus Ankara (MVA) prime-boost regimen. Here, we tested new vaccine candidates based on the recombinant MVA vector, encoding the EBOV nucleoprotein (MVA-EBOV-NP) or glycoprotein (MVA-EBOV-GP) for their efficacy after homologous prime-boost immunization in mice. Our aim was to investigate the role of each antigen in terms of efficacy and correlates of protection. Sera of mice vaccinated with MVA-EBOV-GP were virus-neutralizing and MVA-EBOV-NP immunization readily elicited interferon-γ-producing NP-specific CD8+ T cells. While mock-vaccinated mice succumbed to EBOV infection, all vaccinated mice survived and showed drastically decreased viral loads in sera and organs. In addition, MVA-EBOV-NP vaccinated mice became susceptible to lethal EBOV infection after depletion of CD8+ T cells prior to challenge. This study highlights the potential of MVA-based vaccines to elicit humoral immune responses as well as a strong and protective CD8+ T cell response and contributes to understanding the possible underlying mechanisms.
Language	English
Publishing date	2022-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10040533
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Article ; Online: Multiple Orientia clusters and Th1-skewed chemokine profile: a cross-sectional study in patients with scrub typhus from Nepal.

Münch, Carina Chan-Song / Upadhaya, Bishnu Prashad / Rayamajhee, Binod / Adhikari, Anurag / Münch, Manuel / En-Nosse, Nora / Kowalski, Katharina / Eickmann, Markus / Bauer, Christian / Manandhar, Krishna Das / Keller, Christian

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2022 Volume 128, Page(s) 78–87

Abstract: Objectives: Scrub typhus is an emerging infectious disease in Asia caused by Orientia tsutsugamushi (Ot). From Nepal, only scant data on the genetic epidemiology of this agent is available, and determinants of immunoregulation are poorly understood.: ... ...

Abstract	Objectives: Scrub typhus is an emerging infectious disease in Asia caused by Orientia tsutsugamushi (Ot). From Nepal, only scant data on the genetic epidemiology of this agent is available, and determinants of immunoregulation are poorly understood. Methods: Patients (n = 238) referred to the National Public Health Laboratory (Kathmandu, Nepal) from all over Nepal for suspected scrub typhus were enrolled upon positive immunoglobulin (Ig)M testing between July and October 2015. From Ot 16S and 47 kD polymerase chain reaction (PCR)-positive samples, the variable domain I of the 56 kD gene was sequenced and phylogenetically analyzed. T helper (Th) cell-associated cytokines (n = 13) and chemokines (n = 12) were quantified by multiplex bead arrays. Results: In 93/238 (39.1%) IgM-positive samples, Ot DNA was detected by quantitative PCR. Phylogenetic analysis of 56 kD sequences revealed seven distinct clusters, six of them with high homologies to strains detected in other countries. The Th1-related cytokines interferon-γ and C-X-C motif chemokine ligand 10 were strongly upregulated and correlated with bacteremia, while levels of Th2-associated chemokines were reduced. Bacteremia also correlated with concentrations of interleukin (IL)-6 and IL-10 but not tumor necrosis factor-α. Conclusion: We identified a considerable genetic heterogeneity of human-pathogenic Ot strains circulating in Nepal. Acute Nepalese scrub typhus patients showed strong Th1 but impaired Th2 responses, especially on the chemokine level.
MeSH term(s)	Humans ; Scrub Typhus/epidemiology ; Nepal/epidemiology ; Cross-Sectional Studies ; Orientia ; Phylogeny ; Orientia tsutsugamushi/genetics ; Cytokines/genetics ; Immunoglobulin M
Chemical Substances	Cytokines ; Immunoglobulin M
Language	English
Publishing date	2022-12-22
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2022.12.022
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Article ; Online: Pre-emptive genomic surveillance of emerging ebolaviruses.

Postigo-Hidalgo, Ignacio / Fischer, Carlo / Moreira-Soto, Andres / Tscheak, Patricia / Nagel, Michael / Eickmann, Markus / Drexler, Jan Felix

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

2020 Volume 25, Issue 3

Abstract: Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays ... ...

Abstract	Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.
MeSH term(s)	Base Sequence ; Communicable Diseases, Emerging ; DNA, Viral/analysis ; Ebolavirus/classification ; Ebolavirus/genetics ; Ebolavirus/isolation & purification ; Genome, Viral/genetics ; Hemorrhagic Fever, Ebola/diagnosis ; Humans ; Polymerase Chain Reaction/methods ; RNA, Viral/genetics ; Sensitivity and Specificity ; Sequence Analysis, DNA
Chemical Substances	DNA, Viral ; RNA, Viral
Language	English
Publishing date	2020-01-13
Publishing country	Sweden
Document type	Journal Article
ZDB-ID	1338803-4
ISSN	1560-7917 ; 1025-496X
ISSN (online)	1560-7917
ISSN	1025-496X
DOI	10.2807/1560-7917.ES.2020.25.3.1900765
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