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Article ; Online: Spectrometric Smartphone-Based System for Ibuprofen Quantification in Commercial Dosage Tablets.

Aguirre, Miguel Ángel / Long, Kenneth D / Cunningham, Brian T

2019 Volume 108, Issue 8, Page(s) 2593–2598

Abstract: A rapid and portable analytical methodology has been developed for ibuprofen (IBU) quantification in commercial dosage tablets using a spectrometric smartphone-based system. The analytical methodology employs point-of-use approaches both for sample ... ...

Abstract	A rapid and portable analytical methodology has been developed for ibuprofen (IBU) quantification in commercial dosage tablets using a spectrometric smartphone-based system. The analytical methodology employs point-of-use approaches both for sample preparation and detection, demonstrating its potential utility for portable quality control of pharmaceutical products. In this work, IBU is dissolved in methanol and then treated with a Co(II) aqueous solution, forming a blue complex which is extractable by dispersive liquid-liquid microextraction. Then, the sample's absorption spectrum is directly measured by a spectrometric smartphone-based system using cartridge made of polyoxymethylene for solvent compatibility. The main experimental factors affecting the dispersive liquid-liquid microextraction of Co-IBU complex were optimized using a multivariate analysis. Under optimized conditions, a working range between 20 and 80 μg mL
MeSH term(s)	Analgesics, Non-Narcotic/analysis ; Anti-Inflammatory Agents, Non-Steroidal/analysis ; Ibuprofen/analysis ; Liquid Phase Microextraction/methods ; Smartphone ; Spectrophotometry/methods ; Tablets
Chemical Substances	Analgesics, Non-Narcotic ; Anti-Inflammatory Agents, Non-Steroidal ; Tablets ; Ibuprofen (WK2XYI10QM)
Language	English
Publishing date	2019-03-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3151-3
ISSN	1520-6017 ; 0022-3549
ISSN (online)	1520-6017
ISSN	0022-3549
DOI	10.1016/j.xphs.2019.03.010
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Article: Analysis of Paper-Based Colorimetric Assays With a Smartphone Spectrometer.

Woodburn, Elizabeth V / Long, Kenneth D / Cunningham, Brian T

IEEE sensors journal

2019 Volume 19, Issue 2, Page(s) 508–514

Abstract: We report on the adaptation of a smartphone's rear-facing camera to function as a spectrometer that measures the spectrum of light scattered by common paper-based assay test strips. We utilize a cartridge that enables a linear series of test pads in a ... ...

Abstract	We report on the adaptation of a smartphone's rear-facing camera to function as a spectrometer that measures the spectrum of light scattered by common paper-based assay test strips. We utilize a cartridge that enables a linear series of test pads in a single strip to be swiped past the read head of the instrument while the phone's camera records video. The strip is housed in a custom-fabricated cartridge that slides through the instrument to facilitate illumination with white light from the smartphone's flash LED that is directed through an optical fiber. We demonstrate the ability to detect subtle changes in the scattered spectrum that enables quantitative analysis of single-analyte and multi-analyte strips. The demonstrated capability can be applied to broad classes of paper-based assays in which visual observation of colored strips is not sufficiently quantitative, and for which analysis of red-green-blue pixel values of a camera image are not capable of measuring complex scattered spectra.
Language	English
Publishing date	2019-09-30
Publishing country	United States
Document type	Journal Article
ISSN	1530-437X
ISSN	1530-437X
DOI	10.1109/JSEN.2018.2876631
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Article ; Online: Supported Platinum Nanoparticles Catalyzed Carbon-Carbon Bond Cleavage of Polyolefins: Role of the Oxide Support Acidity.

Lamb, Jessica V / Lee, Yu-Hsuan / Sun, Jiakai / Byron, Carly / Uppuluri, Ritesh / Kennedy, Robert M / Meng, Chao / Behera, Ranjan K / Wang, Yi-Yu / Qi, Long / Sadow, Aaron D / Huang, Wenyu / Ferrandon, Magali S / Scott, Susannah L / Poeppelmeier, Kenneth R / Abu-Omar, Mahdi M / Delferro, Massimiliano

ACS applied materials & interfaces

2024 Volume 16, Issue 9, Page(s) 11361–11376

Abstract: Supported platinum nanoparticle catalysts are known to convert polyolefins to high-quality liquid hydrocarbons using hydrogen under relatively mild conditions. To date, few studies using platinum grafted onto various metal oxide ( ... ...

Abstract	Supported platinum nanoparticle catalysts are known to convert polyolefins to high-quality liquid hydrocarbons using hydrogen under relatively mild conditions. To date, few studies using platinum grafted onto various metal oxide (M
Language	English
Publishing date	2024-02-23
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c15350
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Article ; Online: Digital imaging software versus the "eyeball" method in quantifying steatosis in a liver biopsy.

Long, Jane J / Nijhar, Kieranjeet / Jenkins, Reed T / Yassine, Adham / Motter, Jennifer D / Jackson, Kyle R / Jerman, Stephanie / Besharati, Sepideh / Anders, Robert A / Dunn, Ty B / Marsh, Christopher L / Rayapati, Divya / Lee, David D / Barth, Rolf N / Woodside, Kenneth J / Philosophe, Benjamin

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

2023 Volume 29, Issue 3, Page(s) 268–278

Abstract: Steatotic livers represent a potentially underutilized resource to increase the donor graft pool; however, 1 barrier to the increased utilization of such grafts is the heterogeneity in the definition and the measurement of macrovesicular steatosis (MaS). ...

Abstract	Steatotic livers represent a potentially underutilized resource to increase the donor graft pool; however, 1 barrier to the increased utilization of such grafts is the heterogeneity in the definition and the measurement of macrovesicular steatosis (MaS). Digital imaging software (DIS) may better standardize definitions to study posttransplant outcomes. Using HALO, a DIS, we analyzed 63 liver biopsies, from 3 transplant centers, transplanted between 2016 and 2018, and compared macrovesicular steatosis percentage (%MaS) as estimated by transplant center, donor hospital, and DIS. We also quantified the relationship between DIS characteristics and posttransplant outcomes using log-linear regression for peak aspartate aminotransferase, peak alanine aminotransferase, and total bilirubin on postoperative day 7, as well as logistic regression for early allograft dysfunction. Transplant centers and donor hospitals overestimated %MaS compared with DIS, with better agreement at lower %MaS and less agreement for higher %MaS. No DIS analyzed liver biopsies were calculated to be >20% %MaS; however, 40% of liver biopsies read by transplant center pathologists were read to be >30%. Percent MaS read by HALO was positively associated with peak aspartate aminotransferase (regression coefficient= 1.04 1.08 1.12 , p <0.001), peak alanine aminotransferase (regression coefficient = 1.04 1.08 1.12 , p <0.001), and early allograft dysfunction (OR= 1.10 1.40 1.78 , p =0.006). There was no association between HALO %MaS and total bilirubin on postoperative day 7 (regression coefficient = 0.99 1.01 1.04 , p =0.3). DIS provides reproducible quantification of steatosis that could standardize MaS definitions and identify phenotypes associated with good clinical outcomes to increase the utilization of steatite livers.
MeSH term(s)	Humans ; Alanine Transaminase ; Aspartate Aminotransferases ; Bilirubin ; Biopsy ; Fatty Liver/diagnostic imaging ; Fatty Liver/pathology ; Liver/diagnostic imaging ; Liver/pathology ; Liver Transplantation/methods ; Software ; Image Processing, Computer-Assisted/methods
Chemical Substances	Alanine Transaminase (EC 2.6.1.2) ; Aspartate Aminotransferases (EC 2.6.1.1) ; Bilirubin (RFM9X3LJ49)
Language	English
Publishing date	2023-01-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2006866-9
ISSN	1527-6473 ; 1527-6465
ISSN (online)	1527-6473
ISSN	1527-6465
DOI	10.1097/LVT.0000000000000064
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Article ; Online: Measurement of filtration efficiencies of healthcare and consumer materials using modified respirator fit tester setup.

Long, Kenneth D / Woodburn, Elizabeth V / Berg, Ian C / Chen, Valerie / Scott, William S

PloS one

2020 Volume 15, Issue 10, Page(s) e0240499

Abstract: During the current SARS-CoV-2 pandemic there is unprecedented demand for personal protective equipment (PPE), especially N95 respirators and surgical masks. The ability of SARS-CoV-2 to be transmitted via respiratory droplets from asymptomatic ... ...

Abstract	During the current SARS-CoV-2 pandemic there is unprecedented demand for personal protective equipment (PPE), especially N95 respirators and surgical masks. The ability of SARS-CoV-2 to be transmitted via respiratory droplets from asymptomatic individuals has necessitated increased usage of both N95 respirators in the healthcare setting and masks (both surgical and homemade) in public spaces. These precautions rely on two fundamental principles of transmission prevention: particle filtration and droplet containment. The former is the focus of NIOSH N95 testing guidelines, and the latter is an FDA guideline for respirators and surgical masks. While studies have investigated droplet containment to provide guidance for homemade mask production, limited work has been done to characterize the filtration efficiency (FE) of materials used in home mask making. In this work, we demonstrate the low-cost (<$300) conversion of standard equipment used to fit-test respirators in hospital and industrial settings into a setup that measures quantitative FEs of materials based on NIOSH N95 guidelines, and subsequently measure FEs of materials found in healthcare and consumer spaces. These materials demonstrate significant variability in filtration characteristics, even for visually similar materials. We demonstrate a FE of 96.49% and pressure drop of 25.4 mmH20 for a double-layer of sterilization wrap used in surgical suites and a FE of 90.37% for a combination of consumer-grade materials. The excellent filtration characteristics of the former demonstrate potential utility for emergent situations when N95 respirators are not available, while those of the latter demonstrate that a high FE can be achieved using publicly available materials.
MeSH term(s)	Aerosols ; Air Filters/standards ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/prevention & control ; Coronavirus Infections/virology ; Equipment Safety/instrumentation ; Equipment Safety/methods ; Health Personnel ; Humans ; Masks/standards ; Materials Testing/instrumentation ; Materials Testing/methods ; Occupational Exposure/prevention & control ; Pandemics/prevention & control ; Personal Protective Equipment/standards ; Pneumonia, Viral/prevention & control ; Pneumonia, Viral/virology ; Respiratory Protective Devices/standards ; SARS-CoV-2
Chemical Substances	Aerosols
Keywords	covid19
Language	English
Publishing date	2020-10-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0240499
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Article ; Online: Measurement of filtration efficiencies of healthcare and consumer materials using modified respirator fit tester setup.

Kenneth D Long / Elizabeth V Woodburn / Ian C Berg / Valerie Chen / William S Scott

PLoS ONE, Vol 15, Iss 10, p e

2020 Volume 0240499

Abstract	During the current SARS-CoV-2 pandemic there is unprecedented demand for personal protective equipment (PPE), especially N95 respirators and surgical masks. The ability of SARS-CoV-2 to be transmitted via respiratory droplets from asymptomatic individuals has necessitated increased usage of both N95 respirators in the healthcare setting and masks (both surgical and homemade) in public spaces. These precautions rely on two fundamental principles of transmission prevention: particle filtration and droplet containment. The former is the focus of NIOSH N95 testing guidelines, and the latter is an FDA guideline for respirators and surgical masks. While studies have investigated droplet containment to provide guidance for homemade mask production, limited work has been done to characterize the filtration efficiency (FE) of materials used in home mask making. In this work, we demonstrate the low-cost (<$300) conversion of standard equipment used to fit-test respirators in hospital and industrial settings into a setup that measures quantitative FEs of materials based on NIOSH N95 guidelines, and subsequently measure FEs of materials found in healthcare and consumer spaces. These materials demonstrate significant variability in filtration characteristics, even for visually similar materials. We demonstrate a FE of 96.49% and pressure drop of 25.4 mmH20 for a double-layer of sterilization wrap used in surgical suites and a FE of 90.37% for a combination of consumer-grade materials. The excellent filtration characteristics of the former demonstrate potential utility for emergent situations when N95 respirators are not available, while those of the latter demonstrate that a high FE can be achieved using publicly available materials.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Tumor-derived GCSF Alters Tumor and Systemic Immune System Cell Subset Composition and Signaling.

Matos, Israel / Barvalia, Maunish / Chehal, Manreet K / Robertson, A Gordon / Kulic, Iva / Silva, Jessica A F D / Ranganathan, Abhinandan / Short, Amy / Huang, Yu-Hsuan / Long, Erin / Priatel, John J / Dhanji, Salim / Nelson, Brad H / Krebs, Danielle L / Harder, Kenneth W

Cancer research communications

2023 Volume 3, Issue 3, Page(s) 404–419

Abstract: While immunotherapies such as immune checkpoint blockade and adoptive T-cell therapy improve survival for a subset of human malignancies, many patients fail to respond. Phagocytes including dendritic cells (DC), monocytes, and macrophages (MF) ... ...

Abstract	While immunotherapies such as immune checkpoint blockade and adoptive T-cell therapy improve survival for a subset of human malignancies, many patients fail to respond. Phagocytes including dendritic cells (DC), monocytes, and macrophages (MF) orchestrate innate and adaptive immune responses against tumors. However, tumor-derived factors may limit immunotherapy effectiveness by altering phagocyte signal transduction, development, and activity. Using Cytometry by Time-of-Flight, we found that tumor-derived GCSF altered myeloid cell distribution both locally and systemically. We distinguished a large number of GCSF-induced immune cell subset and signal transduction pathway perturbations in tumor-bearing mice, including a prominent increase in immature neutrophil/myeloid-derived suppressor cell (Neut/MDSC) subsets and tumor-resident PD-L1 Significance: Tumor-derived GCSF leads to systemic immune population changes. GCSF blockade restores immune populations, improves immunotherapy, and reduces tumor size, paralleling human colorectal cancer data. GCSF inhibition may synergize with current immunotherapies to treat GCSF-secreting tumors.
MeSH term(s)	Humans ; Mice ; Animals ; Myeloid Cells ; Myeloid-Derived Suppressor Cells ; Signal Transduction ; Lymphocytes, Tumor-Infiltrating ; Colonic Neoplasms/metabolism
Language	English
Publishing date	2023-03-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2767-9764
ISSN (online)	2767-9764
DOI	10.1158/2767-9764.CRC-22-0278
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Article ; Online: Metastatic acral melanoma treatment outcomes: a systematic review and meta-analysis.

Cho, Kenneth K / Cust, Anne E / Foo, Yun Megan / Long, Georgina V / Menzies, Alexander M / Eslick, Guy D

Melanoma research

2021 Volume 31, Issue 5, Page(s) 482–486

Abstract: Acral melanomas are a unique subset of melanomas occurring on the palms, soles, and nails. There is poor prognosis with surgery alone and no specific guidelines for the treatment of metastatic acral melanoma. This meta-analysis explored the systemic ... ...

Abstract	Acral melanomas are a unique subset of melanomas occurring on the palms, soles, and nails. There is poor prognosis with surgery alone and no specific guidelines for the treatment of metastatic acral melanoma. This meta-analysis explored the systemic therapy outcomes for metastatic acral melanoma. Medline, Pubmed, EMBASE, and the grey literature were searched from 2010 to August 2020 for studies specifying the treatment outcome of metastatic acral melanoma. Studies were assessed by two investigators. A random-effects meta-analysis was performed and pooled Kaplan-Meier curves for progression-free survival and overall survival were created. Critical appraisal was performed using the Newcastle-Ottawa Scale. Nineteen nonrandomized studies were included, comprising 646 patients with acral melanomas and 1609 patients with nonacral melanomas treated with systemic therapy including chemotherapy, KIT-targeted drugs, as well as anti-CTLA-4 and anti-PD-1 checkpoint inhibitor therapy. Thirteen studies included Kaplan-Meier curves for progression-free survival or overall survival and 11 studies reported treatment responses. Patients with acral melanomas had worse prognosis than nonacral cutaneous melanoma (acral overall survival: median 15 months, 95% CI, 13.7-16.3 months; nonacral cutaneous: median 24 months, 95% CI, 22.6-25.4 months, P < 0.001). Acral melanoma patients treated with anti-PD-1 monotherapy had higher overall survival at 12 months (53%) compared with anti-CTLA-4 monotherapy (34%; P < 0.001). This study provides estimates of treatment response for metastatic acral melanoma, demonstrating low activity across a breadth of approved drug therapies, including anti-PD-1, the most active therapy in melanoma to date. Further research into treatments for metastatic acral melanoma is needed.
MeSH term(s)	Humans ; Immune Checkpoint Inhibitors/therapeutic use ; Melanoma/drug therapy ; Melanoma/immunology ; Melanoma/pathology ; Skin Neoplasms/drug therapy ; Skin Neoplasms/immunology ; Skin Neoplasms/pathology ; Treatment Outcome
Chemical Substances	Immune Checkpoint Inhibitors
Language	English
Publishing date	2021-08-25
Publishing country	England
Document type	Journal Article ; Meta-Analysis ; Research Support, Non-U.S. Gov't ; Systematic Review
ZDB-ID	1095779-0
ISSN	1473-5636 ; 0960-8931
ISSN (online)	1473-5636
ISSN	0960-8931
DOI	10.1097/CMR.0000000000000764
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Article ; Online: Protozoan-Viral-Bacterial Co-Infections Alter Galectin Levels and Associated Immunity Mediators in the Female Genital Tract.

Fichorova, Raina N / DeLong, Allison K / Cu-Uvin, Susan / King, Caroline C / Jamieson, Denise J / Klein, Robert S / Sobel, Jack D / Vlahov, David / Yamamoto, Hidemi S / Mayer, Kenneth H

Frontiers in cellular and infection microbiology

2021 Volume 11, Page(s) 649940

Abstract: Co-infections with sexually transmittable pathogens are common and more likely in women with disturbed vaginal bacteriome. Among those pathogens, the protozoan ... ...

Abstract	Co-infections with sexually transmittable pathogens are common and more likely in women with disturbed vaginal bacteriome. Among those pathogens, the protozoan parasite
MeSH term(s)	Bacteria ; Coinfection ; Female ; Galectin 3 ; Humans ; Prevotella ; Trichomonas Vaginitis ; Virus Diseases
Chemical Substances	Galectin 3
Language	English
Publishing date	2021-08-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2021.649940
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Article ; Online: Point-of-use detection of ascorbic acid using a spectrometric smartphone-based system.

Aguirre, Miguel Ángel / Long, Kenneth D / Canals, Antonio / Cunningham, Brian T

Food chemistry

2018 Volume 272, Page(s) 141–147

Abstract: A rapid and portable analytical methodology has been developed for ascorbic acid (Vitamin C) quantification from aqueous samples using a spectrometric smartphone-based system for the first time. The method employs point-of-use approaches both for sample ... ...

Abstract	A rapid and portable analytical methodology has been developed for ascorbic acid (Vitamin C) quantification from aqueous samples using a spectrometric smartphone-based system for the first time. The method employs point-of-use approaches both for sample preparation and sample measurement, demonstrating the capability for mobile quality control of pharmaceutical and food products. Our approach utilizes an oxidation-reduction reaction between ascorbic acid and methylene blue, followed by a dispersive liquid-liquid microextraction (DLLME) to extract the aqueous-phase methylene blue into organic media. Then, a back-extraction procedure is employed to transfer the methylene blue to aqueous media, followed by analysis of the sample's absorption spectrum using the spectrometric smartphone-based system. The DLLME and back-extraction procedures are optimized by use of a two-step multivariate optimization strategy. Finally, vitamin C supplements and orange juice are used as real-world samples to assess the applicability of the smartphone-based method, which is successfully compared with the standard laboratory-based approach.
MeSH term(s)	Ascorbic Acid/analysis ; Ascorbic Acid/chemistry ; Ascorbic Acid/isolation & purification ; Dietary Supplements/analysis ; Fruit and Vegetable Juices/analysis ; Liquid Phase Microextraction ; Methylene Blue/chemistry ; Point-of-Care Systems ; Smartphone ; Spectrum Analysis/methods ; Water/chemistry
Chemical Substances	Water (059QF0KO0R) ; Ascorbic Acid (PQ6CK8PD0R) ; Methylene Blue (T42P99266K)
Language	English
Publishing date	2018-08-09
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2018.08.002
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