LIVIVO - Search results -

Search results

Result 1 - 9 of total 9

Search options

Article ; Online: Getting physical: Material mechanics is an intrinsic cell cue.

Atcha, Hamza / Choi, Yu Suk / Chaudhuri, Ovijit / Engler, Adam J

2023 Volume 30, Issue 6, Page(s) 750–765

Abstract: Advances in biomaterial science have allowed for unprecedented insight into the ability of material cues to influence stem cell function. These material approaches better recapitulate the microenvironment, providing a more realistic ex vivo model of the ... ...

Abstract	Advances in biomaterial science have allowed for unprecedented insight into the ability of material cues to influence stem cell function. These material approaches better recapitulate the microenvironment, providing a more realistic ex vivo model of the cell niche. However, recent advances in our ability to measure and manipulate niche properties in vivo have led to novel mechanobiological studies in model organisms. Thus, in this review, we will discuss the importance of material cues within the cell niche, highlight the key mechanotransduction pathways involved, and conclude with recent evidence that material cues regulate tissue function in vivo.
MeSH term(s)	Mechanotransduction, Cellular ; Cues ; Stem Cells ; Biocompatible Materials
Chemical Substances	Biocompatible Materials
Language	English
Publishing date	2023-06-12
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2375354-7
ISSN	1875-9777 ; 1934-5909
ISSN (online)	1875-9777
ISSN	1934-5909
DOI	10.1016/j.stem.2023.05.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6589: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 75: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: AP-1 signaling modulates cardiac fibroblast stress responses.

Whitehead, Alexander J / Atcha, Hamza / Hocker, James D / Ren, Bing / Engler, Adam J

Journal of cell science

2023 Volume 136, Issue 23

Abstract: Matrix remodeling outcomes largely dictate patient survival post myocardial infarction. Moreover, human-restricted noncoding regulatory elements have been shown to worsen fibrosis, but their mechanism of action remains elusive. Here, we demonstrate, ... ...

Abstract	Matrix remodeling outcomes largely dictate patient survival post myocardial infarction. Moreover, human-restricted noncoding regulatory elements have been shown to worsen fibrosis, but their mechanism of action remains elusive. Here, we demonstrate, using induced pluripotent stem cell-derived cardiac fibroblasts (iCFs), that inflammatory ligands abundant in the remodeling heart after infarction activate AP-1 transcription factor signaling pathways resulting in fibrotic responses. This observed signaling induces deposition of fibronectin matrix and is further capable of supporting immune cell adhesion; pathway inhibition blocks iCF matrix production and cell adhesion. Polymorphisms in the noncoding regulatory elements within the 9p21 locus (also referred to as ANRIL) redirect stress programs, and in iCFs, they transcriptionally silence the AP-1 inducible transcription factor GATA5. The presence of these polymorphisms modulate iCF matrix production and assembly and reduce cell-cell signaling. These data suggest that this signaling axis is a critical modulator of cardiac disease models and might be influenced by noncoding regulatory elements.
MeSH term(s)	Humans ; Fibroblasts/metabolism ; Fibrosis ; Heart ; Myocardium/metabolism ; Signal Transduction ; Transcription Factor AP-1/genetics ; Transcription Factor AP-1/metabolism
Chemical Substances	Transcription Factor AP-1 ; JunB protein, human
Language	English
Publishing date	2023-12-15
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.261152
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1200: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 14: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Ion channel mediated mechanotransduction in immune cells.

Hamza, Atcha / Amit, Jairaman / Elizabeth L, Evans / Medha M, Pathak / Michael D, Cahalan / Wendy F, Liu

Current opinion in solid state & materials science

2021 Volume 25, Issue 6

Abstract: The immune system performs critical functions to defend against invading pathogens and maintain tissue homeostasis. Immune cells reside within or are recruited to a host of mechanically active tissues throughout the body and, as a result, are exposed to ... ...

Abstract	The immune system performs critical functions to defend against invading pathogens and maintain tissue homeostasis. Immune cells reside within or are recruited to a host of mechanically active tissues throughout the body and, as a result, are exposed to varying types and degrees of mechanical stimuli. Despite their abundance in such tissues, the role of mechanical stimuli in influencing immune cell function and the molecular mechanisms responsible for mechanics-mediated changes are still poorly understood. The recent emergence of mechanically-gated ion channels, particularly Piezo1, has provided an exciting avenue of research within the fields of mechanobiology and immunology. Numerous studies have identified roles for mechanically-gated ion channels in mechanotransduction within various different cell types, with a few recent studies in immune cells. These initial studies provide strong evidence that mechanically-gated ion channels play pivotal roles in regulating the immune system. In this review, we discuss characteristics of ion channel mediated force transduction, review the current techniques used to quantify and visualize ion channel activity in response to mechanical stimuli, and finally we provide an overview of recent studies examining the role of mechanically-gated ion channels in modulating immune cell function.
Language	English
Publishing date	2021-09-15
Publishing country	England
Document type	Journal Article
ISSN	1359-0286
ISSN	1359-0286
DOI	10.1016/j.cossms.2021.100951
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Biophysical regulation of macrophages in health and disease.

Meli, Vijaykumar S / Veerasubramanian, Praveen K / Atcha, Hamza / Reitz, Zachary / Downing, Timothy L / Liu, Wendy F

Journal of leukocyte biology

2019 Volume 106, Issue 2, Page(s) 283–299

Abstract: Macrophages perform critical functions for homeostasis and immune defense in tissues throughout the body. These innate immune cells are capable of recognizing and clearing dead cells and pathogens, and orchestrating inflammatory and healing processes ... ...

Abstract	Macrophages perform critical functions for homeostasis and immune defense in tissues throughout the body. These innate immune cells are capable of recognizing and clearing dead cells and pathogens, and orchestrating inflammatory and healing processes that occur in response to injury. In addition, macrophages are involved in the progression of many inflammatory diseases including cardiovascular disease, fibrosis, and cancer. Although it has long been known that macrophages respond dynamically to biochemical signals in their microenvironment, the role of biophysical cues has only recently emerged. Furthermore, many diseases that involve macrophages are also characterized by changes to the tissue biophysical environment. This review will discuss current knowledge about the effects of biophysical cues including matrix stiffness, material topography, and applied mechanical forces, on macrophage behavior. We will also describe the role of molecules that are known to be important for mechanotransduction, including adhesion molecules, ion channels, as well as nuclear mediators such as transcription factors, scaffolding proteins, and epigenetic regulators. Together, this review will illustrate a developing role of biophysical cues in macrophage biology, and also speculate upon molecular targets that may potentially be exploited therapeutically to treat disease.
MeSH term(s)	Animals ; Cell Adhesion ; Cues ; Disease Susceptibility ; Epigenesis, Genetic ; Extracellular Matrix/metabolism ; Gene Expression Regulation ; Humans ; Immune System Phenomena ; Immunomodulation ; Ion Channels/metabolism ; Lipopolysaccharides/immunology ; Macrophage Activation/immunology ; Macrophages/cytology ; Macrophages/immunology ; Macrophages/metabolism ; Mechanotransduction, Cellular ; Signal Transduction
Chemical Substances	Ion Channels ; Lipopolysaccharides
Language	English
Publishing date	2019-03-12
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.MR0318-126R
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 603: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Mechanically activated ion channel Piezo1 modulates macrophage polarization and stiffness sensing.

Atcha, Hamza / Jairaman, Amit / Holt, Jesse R / Meli, Vijaykumar S / Nagalla, Raji R / Veerasubramanian, Praveen Krishna / Brumm, Kyle T / Lim, Huy E / Othy, Shivashankar / Cahalan, Michael D / Pathak, Medha M / Liu, Wendy F

Nature communications

2021 Volume 12, Issue 1, Page(s) 3256

Abstract: Macrophages perform diverse functions within tissues during immune responses to pathogens and injury, but molecular mechanisms by which physical properties of the tissue regulate macrophage behavior are less well understood. Here, we examine the role of ... ...

Abstract	Macrophages perform diverse functions within tissues during immune responses to pathogens and injury, but molecular mechanisms by which physical properties of the tissue regulate macrophage behavior are less well understood. Here, we examine the role of the mechanically activated cation channel Piezo1 in macrophage polarization and sensing of microenvironmental stiffness. We show that macrophages lacking Piezo1 exhibit reduced inflammation and enhanced wound healing responses. Additionally, macrophages expressing the transgenic Ca
MeSH term(s)	Actins/metabolism ; Animals ; Biocompatible Materials/adverse effects ; Cells, Cultured ; Cellular Microenvironment/immunology ; Disease Models, Animal ; Feedback, Physiological ; Female ; Foreign-Body Reaction/immunology ; Humans ; Ion Channels/genetics ; Ion Channels/metabolism ; Macrophage Activation ; Macrophages/immunology ; Macrophages/metabolism ; Male ; Mechanotransduction, Cellular/immunology ; Mice ; Primary Cell Culture ; Subcutaneous Tissue/surgery ; Wound Healing/immunology
Chemical Substances	Actins ; Biocompatible Materials ; Ion Channels ; Piezo1 protein, mouse
Language	English
Publishing date	2021-05-31
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-021-23482-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Crosstalk Between CD11b and Piezo1 Mediates Macrophage Responses to Mechanical Cues.

Atcha, Hamza / Meli, Vijaykumar S / Davis, Chase T / Brumm, Kyle T / Anis, Sara / Chin, Jessica / Jiang, Kevin / Pathak, Medha M / Liu, Wendy F

Frontiers in immunology

2021 Volume 12, Page(s) 689397

Abstract: Macrophages are versatile cells of the innate immune system that perform diverse functions by responding to dynamic changes in their microenvironment. While the effects of soluble cues, including cytokines and chemokines, have been widely studied, the ... ...

Abstract	Macrophages are versatile cells of the innate immune system that perform diverse functions by responding to dynamic changes in their microenvironment. While the effects of soluble cues, including cytokines and chemokines, have been widely studied, the effects of physical cues, including mechanical stimuli, in regulating macrophage form and function are less well understood. In this study, we examined the effects of static and cyclic uniaxial stretch on macrophage inflammatory and healing activation. We found that cyclic stretch altered macrophage morphology and responses to IFNγ/LPS and IL4/IL13. Interestingly, we found that both static and cyclic stretch suppressed IFNγ/LPS induced inflammation. In contrast, IL4/IL13 mediated healing responses were suppressed with cyclic but enhanced with static stretch conditions. Mechanistically, both static and cyclic stretch increased expression of the integrin CD11b (α
MeSH term(s)	Animals ; CD11b Antigen/immunology ; Cell Survival ; Cells, Cultured ; Female ; Ion Channels/immunology ; Macrophage Activation ; Macrophages/immunology ; Mechanotransduction, Cellular ; Mice, Inbred C57BL ; Mice, Transgenic ; Mice
Chemical Substances	CD11b Antigen ; Ion Channels ; Piezo1 protein, mouse
Language	English
Publishing date	2021-09-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.689397
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mechanically activated ion channel Piezo1 modulates macrophage polarization and stiffness sensing

Hamza Atcha / Amit Jairaman / Jesse R. Holt / Vijaykumar S. Meli / Raji R. Nagalla / Praveen Krishna Veerasubramanian / Kyle T. Brumm / Huy E. Lim / Shivashankar Othy / Michael D. Cahalan / Medha M. Pathak / Wendy F. Liu

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Volume 14

Abstract: Macrophages perform diverse functions during immune responses, but the molecular mechanisms by which physical properties of the tissue regulate macrophage behavior remain unknown. Here the authors find that Piezo1 is a mechanosensor of stiffness, and ... ...

Abstract	Macrophages perform diverse functions during immune responses, but the molecular mechanisms by which physical properties of the tissue regulate macrophage behavior remain unknown. Here the authors find that Piezo1 is a mechanosensor of stiffness, and that its activity modulates macrophage polarization responses.
Keywords	Science ; Q
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: YAP-mediated mechanotransduction tunes the macrophage inflammatory response.

Meli, Vijaykumar S / Atcha, Hamza / Veerasubramanian, Praveen Krishna / Nagalla, Raji R / Luu, Thuy U / Chen, Esther Y / Guerrero-Juarez, Christian F / Yamaga, Kosuke / Pandori, William / Hsieh, Jessica Y / Downing, Timothy L / Fruman, David A / Lodoen, Melissa B / Plikus, Maksim V / Wang, Wenqi / Liu, Wendy F

Science advances

2020 Volume 6, Issue 49

Abstract: Macrophages are innate immune cells that adhere to the extracellular matrix within tissues. However, how matrix properties regulate their function remains poorly understood. Here, we report that the adhesive microenvironment tunes the macrophage ... ...

Abstract	Macrophages are innate immune cells that adhere to the extracellular matrix within tissues. However, how matrix properties regulate their function remains poorly understood. Here, we report that the adhesive microenvironment tunes the macrophage inflammatory response through the transcriptional coactivator YAP. We find that adhesion to soft hydrogels reduces inflammation when compared to adhesion on stiff materials and is associated with reduced YAP expression and nuclear localization. Substrate stiffness and cytoskeletal polymerization, but not adhesive confinement nor contractility, regulate YAP localization. Furthermore, depletion of YAP inhibits macrophage inflammation, whereas overexpression of active YAP increases inflammation. Last, we show in vivo that soft materials reduce expression of inflammatory markers and YAP in surrounding macrophages when compared to stiff materials. Together, our studies identify YAP as a key molecule for controlling inflammation and sensing stiffness in macrophages and may have broad implications in the regulation of macrophages in health and disease.
MeSH term(s)	Extracellular Matrix/metabolism ; Humans ; Inflammation/metabolism ; Macrophages ; Mechanotransduction, Cellular/physiology ; YAP-Signaling Proteins
Chemical Substances	YAP-Signaling Proteins
Language	English
Publishing date	2020-12-04
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.abb8471
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A Low-Cost Mechanical Stretching Device for Uniaxial Strain of Cells: A Platform for Pedagogy in Mechanobiology.

Atcha, Hamza / Davis, Chase T / Sullivan, Nicholas R / Smith, Tim D / Anis, Sara / Dahbour, Waleed Z / Robinson, Zachery R / Grosberg, Anna / Liu, Wendy F

Journal of biomechanical engineering

2018 Volume 140, Issue 8

Abstract: Mechanical cues including stretch, compression, and shear stress play a critical role in regulating the behavior of many cell types, particularly those that experience substantial mechanical stress within tissues. Devices that impart mechanical ... ...

Abstract	Mechanical cues including stretch, compression, and shear stress play a critical role in regulating the behavior of many cell types, particularly those that experience substantial mechanical stress within tissues. Devices that impart mechanical stimulation to cells in vitro have been instrumental in helping to develop a better understanding of how cells respond to mechanical forces. However, these devices often have constraints, such as cost and limited functional capabilities, that restrict their use in research or educational environments. Here, we describe a low-cost method to fabricate a uniaxial cell stretcher that would enable widespread use and facilitate engineering design and mechanobiology education for undergraduate students. The device is capable of producing consistent and reliable strain profiles through the use of a servomotor, gear, and gear rack system. The servomotor can be programmed to output various waveforms at specific frequencies and stretch amplitudes by controlling the degree of rotation, speed, and acceleration of the servogear. In addition, the stretchable membranes are easy to fabricate and can be customized, allowing for greater flexibility in culture well size. We used the custom-built stretching device to uniaxially strain macrophages and cardiomyocytes, and found that both cell types displayed functional and cell shape changes that were consistent with the previous studies using commercially available systems. Overall, this uniaxial cell stretcher provides a more cost-effective alternative to study the effects of mechanical stretch on cells, and can therefore, be widely used in research and educational environments to broaden the study and pedagogy of cell mechanobiology.
MeSH term(s)	Animals ; Biomechanical Phenomena ; Biophysics/education ; Cells ; Costs and Cost Analysis ; Rats ; Stress, Mechanical ; Teaching
Language	English
Publishing date	2018-07-02
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	243094-0
ISSN	1528-8951 ; 0148-0731
ISSN (online)	1528-8951
ISSN	0148-0731
DOI	10.1115/1.4039949
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2672: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito