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Article ; Online: Inclusion of chia seeds (Salvia hispanica L.) and pumpkin seeds (Cucurbita moschata) in dairy sheep diets.

Robles Jimenez, Lizbeth E / Aguirre, Edgar Aranda / Cruz, Maria de Los Angeles Colin / Schettino-Bermúdez, Beatriz / Gutiérrez-Tolentino, Rey / Chay-Canul, Alfonso J / Garcia-Herrera, Ricardo A / Ghavipanje, Navid / Castelan Ortega, Octavio A / Vargas-Bello-Pérez, Einar / Gonzalez-Ronquillo, Manuel

PloS one

2024 Volume 19, Issue 4, Page(s) e0300864

Abstract: Chia (Salvia hispanica L.) seed (CS) and Pumpkin (Cucurbita moschata) seed (PS) are used ...

Abstract	Chia (Salvia hispanica L.) seed (CS) and Pumpkin (Cucurbita moschata) seed (PS) are used in ruminant diets as energy sources. The current experiment studied the impact of dietary inclusion of CS and PS on nutrient intake and digestibility, milk yield, and milk composition of dairy sheep. Twelve primiparous Texel × Suffolk ewes [70 ± 5 days in milk (DIM); 0.320 ± 0.029 kg milk yield] were distributed in a 4 × 3 Latin square design and fed either a butter-based control diet [CON; 13 g/kg dry matter] or two diets with 61 g/kg DM of either CS or PS. Dietary inclusion of CS and PS did not alter live weight (p >0.1) and DM intake (p >0.1). However, compared to the CON, dietary inclusion of both CS and PS increased the digestibility of neutral detergent fiber (p <0.001) and acid detergent lignin (p < 0.001). Milk production (p = 0.001), fat-corrected milk (p < 0.001), and feed efficiency (p < 0.001) were enhanced with PS, while the highest milk protein yield (p < 0.05) and lactose yield (p < 0.001) were for CS-fed ewes. Compared to the CON diet, the ingestion of either CS and/or PS decreased (p < 0.001) the C16:0 in milk. Moreover, both CS and PS tended to enhance the content of C18:3n6 (p > 0.05) and C18:3n3 (p > 0.05). Overall short-term feeding of CS and/or PS (up to 6.1% DM of diet) not only maintains the production performance and digestibility of nutrients but also positively modifies the milk FA composition.
MeSH term(s)	Animals ; Female ; Sheep ; Cucurbita/metabolism ; Lactation ; Salvia hispanica ; Detergents ; Dietary Fiber/metabolism ; Diet/veterinary ; Seeds/metabolism ; Digestion ; Animal Feed/analysis ; Zea mays/metabolism ; Dietary Supplements/analysis ; Rumen/metabolism
Chemical Substances	Detergents ; Dietary Fiber
Language	English
Publishing date	2024-04-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0300864
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Article ; Online: Chemical profile and antifungal activity of plant extracts on Colletotrichum spp. isolated from fruits of Pimenta dioica (L.) Merr.

Velázquez Silva, Aidé / Robles Yerena, Leticia / Barrera Necha, Laura Leticia

Pesticide biochemistry and physiology

2021 Volume 179, Page(s) 104949

Abstract: Pimenta dioica (L.) Merr. is a popular seasoning species. During its production, there are losses ...

Abstract	Pimenta dioica (L.) Merr. is a popular seasoning species. During its production, there are losses of up to 50% due to anthracnose caused by fungi of the genus Colletotrichum. In this study, we evaluated the effect of ethanolic extracts on the in vitro and in vivo control of isolated Colletotrichum spp. Allspice. Extracts of leaves of P. dioica, Piper auritum, Plectranthus amboinicus and Psidium guajava were chemically characterized by gas chromatography and high performance liquid chromatography. Several monoterpenes, sesquiterpenes, flavonoids, organic acids, alkaloids and monoterpenoid phenols were identified among other organic compounds. Alpha-ocimene in P. dioica; alpha-thujene and isosafrole in P. auritum; kumatakenin, cirsilineol, taxifolin and retusin in P. amboinicus are reported for the first time. The extracts of P. amboinicus and P. dioica inhibited mycelial growth, spore germination in Colletotrichum spp. at concentrations of 5, 10 and 20 mg/mL, while the extracts of P. guajava and P. auritum did so at concentrations of 30 and 40 mg/mL. The extracts of P. amboinicus, P. dioica and P. auritum modified the size of the spores, caused degradation and formation of vacuoles in the spores of C. acutatum and C. gloeosporioides. The incidences in fruit inoculated with C. boninense and treated with the extracts of P. amboinicus and P. dioica were of 12% and 4% respectively. The severity was 1.5% with the extract of P. amboinicus and 0% with P. dioica. The results show the richness of bioactive compounds present in the extracts and their possible application to develop biotechnological products against phytopathogenic fungi.
MeSH term(s)	Antifungal Agents/pharmacology ; Colletotrichum ; Fruit ; Genus Pimenta ; Plant Extracts/pharmacology
Chemical Substances	Antifungal Agents ; Plant Extracts
Language	English
Publishing date	2021-08-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	184819-7
ISSN	1095-9939 ; 0048-3583 ; 0048-3575
ISSN (online)	1095-9939
ISSN	0048-3583 ; 0048-3575
DOI	10.1016/j.pestbp.2021.104949
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Article ; Online: Improvement of Fucosylated Oligosaccharides Synthesis by α-L-Fucosidase from Thermotoga maritima in Water-Organic Cosolvent Reaction System.

Robles-Arias, Mónica A / García-Garibay, Mariano / Alatorre-Santamaría, Sergio / Tello-Solís, Salvador R / Guzmán-Rodriguez, Francisco / Gómez-Ruiz, Lorena / Rodríguez-Serrano, Gabriela / Cruz-Guerrero, Alma E

Applied biochemistry and biotechnology

2021 Volume 193, Issue 11, Page(s) 3553–3569

Abstract: The effects of water activity ( ... ...

Abstract	The effects of water activity (a
MeSH term(s)	Bacterial Proteins/chemistry ; Fucose/chemistry ; Oligosaccharides/chemical synthesis ; Solvents/chemistry ; Thermotoga maritima/enzymology ; Water/chemistry ; alpha-L-Fucosidase/chemistry
Chemical Substances	Bacterial Proteins ; Oligosaccharides ; Solvents ; Water (059QF0KO0R) ; Fucose (28RYY2IV3F) ; alpha-L-Fucosidase (EC 3.2.1.51)
Language	English
Publishing date	2021-07-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	392344-7
ISSN	1559-0291 ; 0273-2289
ISSN (online)	1559-0291
ISSN	0273-2289
DOI	10.1007/s12010-021-03628-3
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Article ; Online: Differential response of Oryza sativa L. and Phragmites australis L. plants in trace elements contaminated soils under flooded and unflooded conditions.

Álvarez-Robles, M J / Bernal, M P / Clemente, R

Environmental geochemistry and health

2021 Volume 44, Issue 1, Page(s) 99–115

Abstract: Drastic changes in the water regime of trace elements (TEs) contaminated soils under semiarid conditions, from completely dry to flooding situations, may alter the solubility of the contaminants and, therefore, their potential mobility and availability ... ...

Abstract	Drastic changes in the water regime of trace elements (TEs) contaminated soils under semiarid conditions, from completely dry to flooding situations, may alter the solubility of the contaminants and, therefore, their potential mobility and availability to plants. Certain macrophyte species have shown a promising suitability for their use in the phytoremediation of TEs contaminated soils under fluctuating flooded-unflooded conditions, as a consequence of their high resistance and tolerance to contamination. Similarly, different water conditions occur during rice (Oryza sativa) cultivation, a species often used as a model plant for TEs toxicity studies. The aim of this work was to study the tolerance and oxidative response to TEs of common reed (Phragmites australis) and rice grown in contaminated mining soils, when exposed to different water saturation conditions. Both species (common reed and rice) were cultivated in three different contaminated soils from the Sierra Minera of La Unión-Cartagena (SE-Spain) under contrasting water saturation conditions (flooded and unflooded) in a pot experiment. Soil EC and elevated metal (mainly Cd and Zn) soluble concentrations conditioned the survival of the plants. Whereas, As accumulation in the aerial part of both species influenced the most oxidative stress homeostasis. Common reed showed to be a good candidate for its use in the phytostabilization of TEs contaminated soils under both flooded and unflooded conditions.
MeSH term(s)	Oryza ; Poaceae ; Soil ; Soil Pollutants/analysis ; Soil Pollutants/toxicity ; Trace Elements
Chemical Substances	Soil ; Soil Pollutants ; Trace Elements
Language	English
Publishing date	2021-06-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	52039-1
ISSN	1573-2983 ; 0142-7245 ; 0269-4042
ISSN (online)	1573-2983
ISSN	0142-7245 ; 0269-4042
DOI	10.1007/s10653-021-00979-y
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Article ; Online: Effects of ascorbic acid addition on the oxidative stress response of Oryza sativa L. plants to As(V) exposure.

Álvarez-Robles, M J / Clemente, R / Ferrer, M A / Calderón, A / Bernal, M P

Plant physiology and biochemistry : PPB

2022 Volume 186, Page(s) 232–241

Abstract: Accumulation of noxious elements in the edible part of crops and its impact on food safety is of increasing concern. Rice is one of the major staple food crops worldwide, including arsenic (As)-polluted areas, in which dietary As exposure is becoming a ... ...

Abstract	Accumulation of noxious elements in the edible part of crops and its impact on food safety is of increasing concern. Rice is one of the major staple food crops worldwide, including arsenic (As)-polluted areas, in which dietary As exposure is becoming a widespread health threat. Plant chemical priming has been shown to be an effective strategy to enhance tolerance to environmental stresses, including metal(loid) exposure. The priming effect of ascorbic acid (AsA) was assessed in rice seedlings exposed to As(V) in a hydroponics experiment. AsA treatment (co-addition to the growing media concomitantly (t
MeSH term(s)	Antioxidants/metabolism ; Arsenic/toxicity ; Ascorbic Acid/pharmacology ; Oryza/metabolism ; Oxidative Stress ; Plant Roots/metabolism ; Seedlings/metabolism
Chemical Substances	Antioxidants ; Arsenic (N712M78A8G) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2022-07-20
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2022.07.013
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Article ; Online: Solution-Blown Poly(hydroxybutyrate) and ε-Poly-l-lysine Submicro- and Microfiber-Based Sustainable Nonwovens with Antimicrobial Activity for Single-Use Applications.

Dias, Yasmin Juliane / Robles, Jaqueline Rojas / Sinha-Ray, Suman / Abiade, Jeremiah / Pourdeyhimi, Behnam / Niemczyk-Soczynska, Beata / Kolbuk, Dorota / Sajkiewicz, Pawel / Yarin, Alexander L

ACS biomaterials science & engineering

2021 Volume 7, Issue 8, Page(s) 3980–3992

Abstract: ... Here, sustainable poly(hydroxybutyrate) (PHB) and ε-poly-l-lysine (ε-PLL) submicro- and microfiber ...

Abstract	Antimicrobial nonwovens for single use applications (e.g., diapers, sanitary napkins, medical gauze, etc.) are of utmost importance as the first line of defense against bacterial infections. However, the utilization of petrochemical nondegradable polymers in such nonwovens creates sustainability-related issues. Here, sustainable poly(hydroxybutyrate) (PHB) and ε-poly-l-lysine (ε-PLL) submicro- and microfiber-based antimicrobial nonwovens produced by a novel industrially scalable process, solution blowing, have been proposed. In such nonwovens, ε-PLL acts as an active material. In particular, it was found that most of ε-PLL is released within the first hour of deployment, as is desirable for the applications of interest. The submicro- and microfiber mat was tested against
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents/pharmacology ; Humans ; Hydroxybutyrates/pharmacology ; Polylysine ; Polymers
Chemical Substances	Anti-Bacterial Agents ; Anti-Infective Agents ; Hydroxybutyrates ; Polymers ; Polylysine (25104-18-1)
Language	English
Publishing date	2021-07-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.1c00594
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Article: Differential response of Oryza sativa L. and Phragmites australis L. plants in trace elements contaminated soils under flooded and unflooded conditions

Álvarez-Robles, M. J. / Bernal, M. P. / Clemente, R.

Environmental geochemistry and health. 2022 Jan., v. 44, no. 1

2022

Abstract	Drastic changes in the water regime of trace elements (TEs) contaminated soils under semiarid conditions, from completely dry to flooding situations, may alter the solubility of the contaminants and, therefore, their potential mobility and availability to plants. Certain macrophyte species have shown a promising suitability for their use in the phytoremediation of TEs contaminated soils under fluctuating flooded–unflooded conditions, as a consequence of their high resistance and tolerance to contamination. Similarly, different water conditions occur during rice (Oryza sativa) cultivation, a species often used as a model plant for TEs toxicity studies. The aim of this work was to study the tolerance and oxidative response to TEs of common reed (Phragmites australis) and rice grown in contaminated mining soils, when exposed to different water saturation conditions. Both species (common reed and rice) were cultivated in three different contaminated soils from the Sierra Minera of La Unión-Cartagena (SE-Spain) under contrasting water saturation conditions (flooded and unflooded) in a pot experiment. Soil EC and elevated metal (mainly Cd and Zn) soluble concentrations conditioned the survival of the plants. Whereas, As accumulation in the aerial part of both species influenced the most oxidative stress homeostasis. Common reed showed to be a good candidate for its use in the phytostabilization of TEs contaminated soils under both flooded and unflooded conditions.
Keywords	Oryza sativa ; Phragmites australis ; aerial parts ; geochemistry ; homeostasis ; macrophytes ; oxidative stress ; phytoremediation ; rice ; solubility ; toxicity
Language	English
Dates of publication	2022-01
Size	p. 99-115.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	52039-1
ISSN	1573-2983 ; 0142-7245 ; 0269-4042
ISSN (online)	1573-2983
ISSN	0142-7245 ; 0269-4042
DOI	10.1007/s10653-021-00979-y
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Article ; Online: Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.

Ranea-Robles, Pablo / Violante, Sara / Argmann, Carmen / Dodatko, Tetyana / Bhattacharya, Dipankar / Chen, Hongjie / Yu, Chunli / Friedman, Scott L / Puchowicz, Michelle / Houten, Sander M

Cellular and molecular life sciences : CMLS

2021 Volume 78, Issue 14, Page(s) 5631–5646

Abstract: ... in the peroxisomal L-bifunctional protein (Ehhadh KO mice). In vitro experiments using HEK-293 KO cell lines ...

Abstract	Peroxisomes play an essential role in the β-oxidation of dicarboxylic acids (DCAs), which are metabolites formed upon ω-oxidation of fatty acids. Genetic evidence linking transporters and enzymes to specific DCA β-oxidation steps is generally lacking. Moreover, the physiological functions of DCA metabolism remain largely unknown. In this study, we aimed to characterize the DCA β-oxidation pathway in human cells, and to evaluate the biological role of DCA metabolism using mice deficient in the peroxisomal L-bifunctional protein (Ehhadh KO mice). In vitro experiments using HEK-293 KO cell lines demonstrate that ABCD3 and ACOX1 are essential in DCA β-oxidation, whereas both the bifunctional proteins (EHHADH and HSD17B4) and the thiolases (ACAA1 and SCPx) have overlapping functions and their contribution may depend on expression level. We also show that medium-chain 3-hydroxydicarboxylic aciduria is a prominent feature of EHHADH deficiency in mice most notably upon inhibition of mitochondrial fatty acid oxidation. Using stable isotope tracing methodology, we confirmed that products of peroxisomal DCA β-oxidation can be transported to mitochondria for further metabolism. Finally, we show that, in liver, Ehhadh KO mice have increased mRNA and protein expression of cholesterol biosynthesis enzymes with decreased (in females) or similar (in males) rate of cholesterol synthesis. We conclude that EHHADH plays an essential role in the metabolism of medium-chain DCAs and postulate that peroxisomal DCA β-oxidation is a regulator of hepatic cholesterol biosynthesis.
MeSH term(s)	Animals ; Cholesterol/metabolism ; Dicarboxylic Acids/urine ; Female ; HEK293 Cells ; Homeostasis ; Humans ; Lipid Metabolism, Inborn Errors/etiology ; Lipid Metabolism, Inborn Errors/pathology ; Liver Diseases/etiology ; Liver Diseases/metabolism ; Liver Diseases/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism ; Mitochondria/pathology ; Peroxisomal Bifunctional Enzyme/physiology
Chemical Substances	Dicarboxylic Acids ; Cholesterol (97C5T2UQ7J) ; Ehhadh protein, mouse (EC 4.2.1.17) ; Peroxisomal Bifunctional Enzyme (EC 4.2.1.17)
Language	English
Publishing date	2021-06-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1358415-7
ISSN	1420-9071 ; 1420-682X
ISSN (online)	1420-9071
ISSN	1420-682X
DOI	10.1007/s00018-021-03869-9
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Article ; Online: Evaluación técnica y económica de un micro huerto de vid (Vitis vinífera, L.) en Delicias, Chihuahua

Michelle Gómez Robles / José Eduardo Magaña Magaña / Jorge Alberto Sánchez Bernal / Fabiola Iveth Ortega Montes / Ma. Guadalupe Macías López

Revista Biológico Agropecuaria Tuxpan, Vol 11, Iss

Primera Etapa

2023 Volume 2

Abstract: El vino mexicano se ha consolidado en el país y en el extranjero como uno de los productos mexicanos más famosos de los últimos años. Los vinos mexicanos son premio internacional, diálogo de expertos y avalancha de buenas noticias, pero también son ... ...

Abstract	El vino mexicano se ha consolidado en el país y en el extranjero como uno de los productos mexicanos más famosos de los últimos años. Los vinos mexicanos son premio internacional, diálogo de expertos y avalancha de buenas noticias, pero también son importantes para la economía nacional. Según la Secretaría de Innovación y Desarrollo Económico (2021), las principales zonas de cultivo de Chihuahua son Bachiniva, Delicias y Sacramento, con vinos de uva blanca como el Chardonnay y Uva tinta como el Cabernet Sauvignon, Merlot, Syrah, Tempranillo y Pinot Noir. El estudio se realizó con el fin de evaluar la factibilidad técnica y económica de un micro huerto en Delicias, Chihuahua. Se colocaron hileras de cuatro variedades (Cabernet Sauvignon, Merlot, Syrah y Tempranillo) junto con el sistema de riego por goteo en los terrenos agrícolas de la Facultad de Ciencias Agrícolas y Forestales de la UACH, en Delicias. A la fecha la microhuerta tiene un desarrollo fisiológico bueno, la variedad más precoz en crecimiento fue Syrah y Tempranillo.
Keywords	Cabernet sauvignon ; merlot ; syrah ; tempranillo ; Veterinary medicine ; SF600-1100 ; Agriculture ; S
Language	Spanish
Publishing date	2023-12-01T00:00:00Z
Publisher	Editorial Agrícola Tuxpan
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Gene Expression Analysis of Microtubers of Potato Solanum tuberosum L. Induced in Cytokinin Containing Medium and Osmotic Stress

Herrera-Isidron, Lisset / Valencia-Lozano, Eliana / Rosiles-Loeza, Pablo Yamild / Robles-Hernández, Maria Guadalupe / Napsuciale-Heredia, Abigail / Cabrera-Ponce, Jose Luis

Plants. 2021 Apr. 27, v. 10, no. 5

2021

Abstract: Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a ... ...

Abstract	Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system (StHK1), cytokinin signaling, (StHK3, StHP4, StRR1) homeodomains (WUSCHEL, POTH1, BEL5), auxin signaling, ARF5, carbon metabolism (TPI, TIM), protein synthesis, NAC5 and a morphogenetic regulator of tuberization (POTH15) was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.
Keywords	Solanum tuberosum ; auxins ; carbon metabolism ; cytokinins ; gene expression ; gene expression regulation ; genes ; osmotic stress ; potatoes ; protein synthesis ; sucrose
Language	English
Dates of publication	2021-0427
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10050876
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