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Article: Influence of viral genome properties on polymerase fidelity.

Dupré, Gabriel / Volmer, Romain

2022 Volume 39, Issue 1, Page(s) 9–14

Abstract: The first step of viral evolution takes place during genome replication via the error-prone viral polymerase. Among the mutants that arise through this process, only a few well-adapted variants will be selected by natural selection, renewing the viral ... ...

Abstract	The first step of viral evolution takes place during genome replication via the error-prone viral polymerase. Among the mutants that arise through this process, only a few well-adapted variants will be selected by natural selection, renewing the viral genome population. Viral polymerase-mediated errors are thought to occur stochastically. However, accumulating evidence suggests that viral polymerase-mediated mutations are heterogeneously distributed throughout the viral genome. Here, we review work that supports this concept and provides mechanistic insights into how specific features of the viral genome could modulate viral polymerase-mediated errors. A predisposition to accumulate viral polymerase-mediated errors at specific loci in the viral genome may guide evolution to specific pathways, thus opening new directions of research to better understand viral evolutionary dynamics.
MeSH term(s)	Mutation ; Genome, Viral/genetics ; Genotype
Language	English
Publishing date	2022-11-16
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	619240-3
ISSN	1362-4555 ; 0168-9525 ; 0168-9479
ISSN (online)	1362-4555
ISSN	0168-9525 ; 0168-9479
DOI	10.1016/j.tig.2022.10.008
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: From one to many: The within-host rise of viral variants.

Bessière, Pierre / Volmer, Romain

PLoS pathogens

2021 Volume 17, Issue 9, Page(s) e1009811

MeSH term(s)	Genetic Variation ; Humans ; Virus Diseases/genetics ; Virus Diseases/immunology ; Viruses/genetics
Language	English
Publishing date	2021-09-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1009811
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: From one to many

Pierre Bessière / Romain Volmer

PLoS Pathogens, Vol 17, Iss 9, p e

The within-host rise of viral variants.

2021 Volume 1009811

Keywords	Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Controlling the emergence of highly pathogenic avian influenza viruses: one of the challenges of the 21st century?

Bessière, Pierre / Dupré, Gabriel / Volmer, Romain

Virologie (Montrouge, France)

2022 Volume 26, Issue 5, Page(s) 343–354

Abstract: Millions of farmed birds culled, hundreds of gannets dead on the coast, dying marine mammals suffering from neurological disorders: these events regularly make the headlines. What do they have in common? Highly pathogenic avian influenza viruses (HPAIV). ...

Title translation	La lutte contre l’émergence des virus influenza aviaires hautement pathogènes : un des défis du XXIe siècle ?
Abstract	Millions of farmed birds culled, hundreds of gannets dead on the coast, dying marine mammals suffering from neurological disorders: these events regularly make the headlines. What do they have in common? Highly pathogenic avian influenza viruses (HPAIV). HPAIVs are viruses capable of replicating systemically, causing both asymptomatic infections and devastating mortality, depending on the susceptibility of the host species. Known for several decades now, these viruses have seen their circulation particularly increased in recent years, and have been responsible for massive epizootics on several continents. In addition to the devastating effects they can cause in poultry and wildlife, HPAIVs are also capable of crossing the species barrier. Improving knowledge about these viruses and better control of their spread therefore has several objectives: to protect public health, to guarantee food safety, to preserve biodiversity and the economy of the poultry industry. This article reviews the current state of knowledge on HPAIVs: from their epidemiology to the mechanisms of emergence and control measures.
MeSH term(s)	Animals ; Influenza in Birds/epidemiology ; Influenza in Birds/prevention & control ; Influenza in Birds/pathology ; Influenza A virus ; Poultry ; Birds ; Animals, Wild ; Mammals
Language	French
Publishing date	2022-11-22
Publishing country	France
Document type	Review ; English Abstract ; Journal Article
ZDB-ID	2118387-9
ISSN	1950-6961 ; 1267-8694
ISSN (online)	1950-6961
ISSN	1267-8694
DOI	10.1684/vir.2022.0971
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In Vitro

Foret-Lucas, Charlotte / Figueroa, Thomas / Coggon, Amelia / Houffschmitt, Alexandre / Dupré, Gabriel / Fusade-Boyer, Maxime / Guérin, Jean-Luc / Delverdier, Maxence / Bessière, Pierre / Volmer, Romain

Microbiology spectrum

2023 Volume 11, Issue 1, Page(s) e0422922

Abstract: H5N8 high-pathogenicity avian influenza virus (HPAIV) of clade 2.3.4.4B, which circulated during the 2016 epizootics in Europe, was notable for causing different clinical signs in ducks and chickens. The clinical signs preceding death were predominantly ... ...

Abstract	H5N8 high-pathogenicity avian influenza virus (HPAIV) of clade 2.3.4.4B, which circulated during the 2016 epizootics in Europe, was notable for causing different clinical signs in ducks and chickens. The clinical signs preceding death were predominantly neurological in ducks versus respiratory in chickens. To investigate the determinants for the predominant neurological signs observed in ducks, we infected duck and chicken primary cortical neurons. Viral replication was identical in neuronal cultures from both species. In addition, we did not detect any major difference in the immune and inflammatory responses. These results suggest that the predominant neurological involvement of H5N8 HPAIV infection in ducks could not be recapitulated in primary neuronal cultures.
MeSH term(s)	Animals ; Influenza in Birds ; Chickens ; Ducks ; Influenza A Virus, H5N8 Subtype/physiology ; Virulence ; Influenza A virus ; Poultry Diseases ; Respiratory Distress Syndrome
Language	English
Publishing date	2023-01-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.04229-22
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Article ; Online: A New Derivative of Retro-2 Displays Antiviral Activity against Respiratory Syncytial Virus.

Le Rouzic, Adrien / Fix, Jenna / Vinck, Robin / Kappler-Gratias, Sandrine / Volmer, Romain / Gallardo, Franck / Eléouët, Jean-François / Keck, Mathilde / Cintrat, Jean-Christophe / Barbier, Julien / Gillet, Daniel / Galloux, Marie

International journal of molecular sciences

2023 Volume 25, Issue 1

Abstract: Human respiratory syncytial virus (hRSV) is the most common cause of bronchiolitis and pneumonia in newborns, with all children being infected before the age of two. Reinfections are very common throughout life and can cause severe respiratory infections ...

Abstract	Human respiratory syncytial virus (hRSV) is the most common cause of bronchiolitis and pneumonia in newborns, with all children being infected before the age of two. Reinfections are very common throughout life and can cause severe respiratory infections in the elderly and immunocompromised adults. Although vaccines and preventive antibodies have recently been licensed for use in specific subpopulations of patients, there is still no therapeutic treatment commonly available for these infections. Here, we investigated the potential antiviral activity of Retro-2.2, a derivative of the cellular retrograde transport inhibitor Retro-2, against hRSV. We show that Retro-2.2 inhibits hRSV replication in cell culture and impairs the ability of hRSV to form syncytia. Our results suggest that Retro-2.2 treatment affects virus spread by disrupting the trafficking of the viral de novo synthetized F and G glycoproteins to the plasma membrane, leading to a defect in virion morphogenesis. Taken together, our data show that targeting intracellular transport may be an effective strategy against hRSV infection.
MeSH term(s)	Infant, Newborn ; Adult ; Child ; Aged ; Humans ; Respiratory Syncytial Virus, Human ; Respiratory Syncytial Virus Infections/drug therapy ; Respiratory Tract Infections ; Antibodies ; Antiviral Agents/pharmacology
Chemical Substances	Antibodies ; Antiviral Agents
Language	English
Publishing date	2023-12-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25010415
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Article ; Online: EPAC1 Pharmacological Inhibition with AM-001 Prevents SARS-CoV-2 and Influenza A Virus Replication in Cells.

Foret-Lucas, Charlotte / Figueroa, Thomas / Bertin, Alexandre / Bessière, Pierre / Lucas, Alexandre / Bergonnier, Dorian / Wasniewski, Marine / Servat, Alexandre / Tessier, Arnaud / Lezoualc'h, Frank / Volmer, Romain

Viruses

2023 Volume 15, Issue 2

Abstract: The exceptional impact of the COVID-19 pandemic has stimulated an intense search for antiviral molecules. Host-targeted antiviral molecules have the potential of presenting broad-spectrum antiviral activity and are also considered as less likely to ... ...

Abstract	The exceptional impact of the COVID-19 pandemic has stimulated an intense search for antiviral molecules. Host-targeted antiviral molecules have the potential of presenting broad-spectrum antiviral activity and are also considered as less likely to select for resistant viruses. In this study, we investigated the antiviral activity exerted by AM-001, a specific pharmacological inhibitor of EPAC1, a host exchange protein directly activated by cyclic AMP (cAMP). The cAMP-sensitive protein, EPAC1 regulates various physiological and pathological processes but its role in SARS-CoV-2 and influenza A virus infection has not yet been studied. Here, we provide evidence that the EPAC1 specific inhibitor AM-001 exerts potent antiviral activity against SARS-CoV-2 in the human lung Calu-3 cell line and the African green monkey Vero cell line. We observed a concentration-dependent inhibition of SARS-CoV-2 infectious viral particles and viral RNA release in the supernatants of AM-001 treated cells that was not associated with a significant impact on cellular viability. Furthermore, we identified AM-001 as an inhibitor of influenza A virus in Calu-3 cells. Altogether these results identify EPAC1 inhibition as a promising therapeutic target against viral infections.
MeSH term(s)	Humans ; Antiviral Agents/pharmacology ; Chlorocebus aethiops ; COVID-19 ; Influenza A virus ; Influenza, Human/drug therapy ; Pandemics ; RNA, Viral ; SARS-CoV-2 ; Virus Replication
Chemical Substances	Antiviral Agents ; RAPGEF3 protein, human ; RNA, Viral ; EPAC1 inhibitor AM-001
Language	English
Publishing date	2023-01-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15020319
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Article ; Online: Lipid-dependent regulation of the unfolded protein response.

Volmer, Romain / Ron, David

Current opinion in cell biology

2014 Volume 33, Page(s) 67–73

Abstract: Protein folding homeostasis in the lumen of the endoplasmic reticulum is defended by signal transduction pathways that are activated by an imbalance between unfolded proteins and chaperones (so called ER stress). Collectively referred to as the unfolded ... ...

Abstract	Protein folding homeostasis in the lumen of the endoplasmic reticulum is defended by signal transduction pathways that are activated by an imbalance between unfolded proteins and chaperones (so called ER stress). Collectively referred to as the unfolded protein response (UPR) this homeostatic response is initiated by three known ER stress transducers: IRE1, PERK and ATF6. These ER-localised transmembrane (TM) proteins posses lumenal stress sensing domains and cytosolic effector domains that collectively activate a gene expression programme regulating the production of proteins involved in the processing and maturation of secreted proteins that enter the ER. However, beyond limiting unfolded protein stress in the ER the UPR has important connections to lipid metabolism that are the subject of this review.
MeSH term(s)	Animals ; Endoplasmic Reticulum/metabolism ; Eukaryota/metabolism ; Humans ; Lipid Metabolism ; Molecular Chaperones/biosynthesis ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Protein Folding ; Signal Transduction ; Unfolded Protein Response
Chemical Substances	Molecular Chaperones
Language	English
Publishing date	2014-12-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1026381-0
ISSN	1879-0410 ; 0955-0674
ISSN (online)	1879-0410
ISSN	0955-0674
DOI	10.1016/j.ceb.2014.12.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 2963: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article: Phylodynamic study of the conserved RNA structure encompassing the hemagglutinin cleavage site encoding region of H5 and H7 low pathogenic avian influenza viruses.

Dupré, Gabriel / Hoede, Claire / Figueroa, Thomas / Bessière, Pierre / Bertagnoli, Stéphane / Ducatez, Mariette / Gaspin, Christine / Volmer, Romain

Virus evolution

2021 Volume 7, Issue 2, Page(s) veab093

Abstract: Highly pathogenic avian influenza viruses (HPAIVs) evolve from low pathogenic avian influenza viruses (LPAIVs) of the H5 and H7 subtypes. This evolution is characterized by the acquisition of a multi-basic cleavage site (MBCS) motif in the hemagglutinin ( ...

Abstract	Highly pathogenic avian influenza viruses (HPAIVs) evolve from low pathogenic avian influenza viruses (LPAIVs) of the H5 and H7 subtypes. This evolution is characterized by the acquisition of a multi-basic cleavage site (MBCS) motif in the hemagglutinin (HA) that leads to an extended viral tropism and severe disease in poultry. One key unanswered question is whether the risk of transition to HPAIVs is similar for all LPAIVs H5 or H7 strains, or whether specific determinants in the HA sequence of some H5 or H7 LPAIV strains correlate with a higher risk of transition to HPAIVs. Here, we determined if specific features of the conserved RNA stem-loop located at the HA cleavage site-encoding region could be detected along the LPAIV to HPAIV evolutionary pathway. Analysis of the thermodynamic stability of the predicted RNA structures showed no specific patterns common to HA sequences leading to HPAIVs and distinct from those remaining LPAIVs. However, RNA structure clustering analysis revealed that most of the American lineage ancestors leading to H7 emergences via recombination shared the same viral RNA (vRNA) structure topology at the HA1/HA2 boundary region. Our study thus identified predicted secondary RNA structures present in the HA of H7 viruses, which could promote genetic recombination and acquisition of a multibasic cleavage site motif (MBCS).
Language	English
Publishing date	2021-11-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2818949-8
ISSN	2057-1577
ISSN	2057-1577
DOI	10.1093/ve/veab093
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A New Derivative of Retro-2 Displays Antiviral Activity against Respiratory Syncytial Virus

Adrien Le Rouzic / Jenna Fix / Robin Vinck / Sandrine Kappler-Gratias / Romain Volmer / Franck Gallardo / Jean-François Eléouët / Mathilde Keck / Jean-Christophe Cintrat / Julien Barbier / Daniel Gillet / Marie Galloux

International Journal of Molecular Sciences, Vol 25, Iss 1, p

2023 Volume 415

Abstract	Human respiratory syncytial virus (hRSV) is the most common cause of bronchiolitis and pneumonia in newborns, with all children being infected before the age of two. Reinfections are very common throughout life and can cause severe respiratory infections in the elderly and immunocompromised adults. Although vaccines and preventive antibodies have recently been licensed for use in specific subpopulations of patients, there is still no therapeutic treatment commonly available for these infections. Here, we investigated the potential antiviral activity of Retro-2.2, a derivative of the cellular retrograde transport inhibitor Retro-2, against hRSV. We show that Retro-2.2 inhibits hRSV replication in cell culture and impairs the ability of hRSV to form syncytia. Our results suggest that Retro-2.2 treatment affects virus spread by disrupting the trafficking of the viral de novo synthetized F and G glycoproteins to the plasma membrane, leading to a defect in virion morphogenesis. Taken together, our data show that targeting intracellular transport may be an effective strategy against hRSV infection.
Keywords	respiratory syncytial virus ; Retro-2.2 ; fusion protein ; retrograde transport ; antiviral ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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