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Article ; Online: Enhanced Mechanical Property of Polyamide-6/Graphite Sheet Composites with Segregated 3D Network Binary Structure for High Thermal Conductivity.

Gao, Yao / Li, Yong / Kong, Xiangwei / Ma, Meng

2023 Volume 15, Issue 4

Abstract: Segregated conductive polymer composites exhibit excellent electrical properties with a low percolation threshold. However, the mechanical properties of the segregated conductive polymer composites were always poor because the conductive fillers at the ... ...

Abstract	Segregated conductive polymer composites exhibit excellent electrical properties with a low percolation threshold. However, the mechanical properties of the segregated conductive polymer composites were always poor because the conductive fillers at the interfaces hinder polymer chain diffusion and thus lead to weak interfacial interaction between the conductive fillers and the polymer matrix. In this paper, polyamide-6 and polyamide-612 microspheres were synthesized via the in situ anionic ring opening of caprolactam and laurolactam. Segregated graphite sheets/polyamide-6(GS/PA6) and polyamide-612(PA612) composites with good mechanical properties were realized via high-pressure solid-phase compression molding. The microstructures of the composite samples were observed by scanning electron microscopy, which showed that the formation of a GS-conductive network at the PA6 granule interfaces in the segregated conductive structures and the adopting of PA612 considerably improved the interfacial adhesion of the composites. A superior impact strength of 5.1 kJ/m
Language	English
Publishing date	2023-02-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym15041041
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Advances in Biosensors for the Rapid Detection of Marine Biotoxins: Current Status and Future Perspectives.

Zhu, Xiangwei / Zhao, Yufa / Wu, Long / Gao, Xin / Huang, Huang / Han, Yu / Zhu, Ting

Biosensors

2024 Volume 14, Issue 4

Abstract: Marine biotoxins (MBs), harmful metabolites of marine organisms, pose a significant threat to marine ecosystems and human health due to their diverse composition and widespread occurrence. Consequently, rapid and efficient detection technology is crucial ...

Abstract	Marine biotoxins (MBs), harmful metabolites of marine organisms, pose a significant threat to marine ecosystems and human health due to their diverse composition and widespread occurrence. Consequently, rapid and efficient detection technology is crucial for maintaining marine ecosystem and human health. In recent years, rapid detection technology has garnered considerable attention for its pivotal role in identifying MBs, with advancements in sensitivity, specificity, and accuracy. These technologies offer attributes such as speed, high throughput, and automation, thereby meeting detection requirements across various scenarios. This review provides an overview of the classification and risks associated with MBs. It briefly outlines the current research status of marine biotoxin biosensors and introduces the fundamental principles, advantages, and limitations of optical, electrochemical, and piezoelectric biosensors. Additionally, the review explores the current applications in the detection of MBs and presents forward-looking perspectives on their development, which aims to be a comprehensive resource for the design and implementation of tailored biosensors for effective MB detection.
MeSH term(s)	Biosensing Techniques ; Marine Toxins/analysis ; Aquatic Organisms ; Humans
Chemical Substances	Marine Toxins
Language	English
Publishing date	2024-04-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios14040203
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Revealing the biological significance of multiple metabolic pathways of chloramphenicol by Sphingobium sp. WTD-1.

Gao, Yongsheng / Chen, Yao / Zhu, Fang / Pan, Dandan / Huang, Junwei / Wu, Xiangwei

Journal of hazardous materials

2024 Volume 469, Page(s) 134069

Abstract: Chloramphenicol (CAP) is an antibiotic that commonly pollutes the environment, and microorganisms primarily drive its degradation and transformation. Although several pathways for CAP degradation have been documented in different bacteria, multiple ... ...

Abstract	Chloramphenicol (CAP) is an antibiotic that commonly pollutes the environment, and microorganisms primarily drive its degradation and transformation. Although several pathways for CAP degradation have been documented in different bacteria, multiple metabolic pathways in the same strain and their potential biological significance have not been revealed. In this study, Sphingobium WTD-1, which was isolated from activated sludge, can completely degrade 100 mg/L CAP within 60 h as the sole energy source. UPLC-HRMS and HPLC analyses showed that three different pathways, including acetylation, hydroxyl oxidation, and oxidation (C1-C2 bond cleavage), are responsible for the metabolism of CAP. Importantly, acetylation and C3 hydroxyl oxidation reduced the cytotoxicity of the substrate to strain WTD-1, and the C1-C2 bond fracture of CAP generated the metabolite p-nitrobenzoic acid (PNBA) to provide energy for its growth. This indicated that the synergistic action of three metabolic pathways caused WTD-1 to be adaptable and able to degrade high concentrations of CAP in the environment. This study deepens our understanding of the microbial degradation pathway of CAP and highlights the biological significance of the synergistic metabolism of antibiotic pollutants by multiple pathways in the same strain.
MeSH term(s)	Chloramphenicol/metabolism ; Biodegradation, Environmental ; Anti-Bacterial Agents/metabolism ; Metabolic Networks and Pathways ; Sphingomonadaceae/metabolism
Chemical Substances	Chloramphenicol (66974FR9Q1) ; Anti-Bacterial Agents
Language	English
Publishing date	2024-03-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2024.134069
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Article: Genome-Wide Identification and Expression Analysis Reveal bZIP Transcription Factors Mediated Hormones That Functions during Early Somatic Embryogenesis in

Zhai, Tingkai / Lan, Shuoxian / Xv, Luzhen / Zhang, Xueying / Ma, Xiangwei / Li, Zhuoyun / Gao, Jie / Chen, Yukun / Lai, Zhongxiong / Lin, Yuling

Plants (Basel, Switzerland)

2024 Volume 13, Issue 5

Abstract: The basic leucine zip (bZIP) transcription factors (TFs) are a group of highly conserved gene families that play important roles in plant growth and resistance to adversity stress. However, studies on hormonal regulatory pathways and functional analysis ... ...

Abstract	The basic leucine zip (bZIP) transcription factors (TFs) are a group of highly conserved gene families that play important roles in plant growth and resistance to adversity stress. However, studies on hormonal regulatory pathways and functional analysis during somatic embryogenesis (SE) in
Language	English
Publishing date	2024-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants13050662
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Article ; Online: Performance of smoke confinement during fire accident in subway station

Luo Na / Gao Le / Liu Xiangwei

E3S Web of Conferences, Vol 356, p

2022 Volume 02036

Abstract: Fire accidents often lead to tremendous losses and smoke has been reported to be the main cause of these heavy casualties. Accordingly, smoke movement can influence escape and smoke confinement is particular significant. Fire Dynamics Simulator software ( ...

Abstract	Fire accidents often lead to tremendous losses and smoke has been reported to be the main cause of these heavy casualties. Accordingly, smoke movement can influence escape and smoke confinement is particular significant. Fire Dynamics Simulator software (FDS) was utilized to carry out complementary full scale numerical simulations of fire accident in a subway station. Different measure of smoke prevention and exhaust were adopted and the difference of smoke confinement among the measure was compared. According to the smoke temperature, hung wall had a good role during early stage of fire accident. However, the performance became worse with smoke accumulation. If the hung wall were adopted, smoke was exhausted from basement 2 and air was supplied into the basement 1 at the same time, the efficacy of smoke confinement was the best. The mechanical exhaust rate had obvious implications for the smoke diffusion.
Keywords	Environmental sciences ; GE1-350
Subject code	690
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Ratiometric SERS quantification of SO

Lu, Yu / Mo, Xiufang / Zhu, Geng / Huang, Yan / Wang, Yingchao / Yang, Zhenzhong / Gao, Liqiong / Shen, Guofang / Wang, Yi / Zhao, Xiangwei

Journal of hazardous materials

2024 Volume 467, Page(s) 133763

Abstract: Practical gas sensing application requires sensors to quantify target analytes with high sensitivity and reproducibility. However, conventional surface enhanced Raman scattering (SERS) sensor lacks reproducibility and quantification arising from ... ...

Abstract	Practical gas sensing application requires sensors to quantify target analytes with high sensitivity and reproducibility. However, conventional surface enhanced Raman scattering (SERS) sensor lacks reproducibility and quantification arising from variations of "hot spot" distribution and measurement conditions. Here, a ratio-dependent SERS sensor was developed for quantitative label-free gas sensing. Au@Ag-Au nanoparticles (NPs) were filtered onto anodic aluminum oxide (AAO) forming Au@Ag-Au@AAO SERS substrate. 4-MBA was encapsulated in the gap of Au@Ag-Au and served as the internal standard (IS) to calibrate SERS signal fluctuation for improved quantification ability. Combined with headspace sampling method, SO
MeSH term(s)	Metal Nanoparticles/chemistry ; Gold/chemistry ; Reproducibility of Results ; Silver/chemistry ; Medicine, Chinese Traditional
Chemical Substances	Gold (7440-57-5) ; Silver (3M4G523W1G)
Language	English
Publishing date	2024-02-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2024.133763
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Article ; Online: RNA modifications in cardiovascular diseases, the potential therapeutic targets.

Wu, Yirong / Zhan, Siyao / Xu, Yizhou / Gao, Xiangwei

Life sciences

2021 Volume 278, Page(s) 119565

Abstract: More than one hundred RNA modifications decorate the chemical and topological properties of these ribose nucleotides, thereby executing their biological functions through post-transcriptional regulation. In cardiovascular diseases, a wide range of RNA ... ...

Abstract	More than one hundred RNA modifications decorate the chemical and topological properties of these ribose nucleotides, thereby executing their biological functions through post-transcriptional regulation. In cardiovascular diseases, a wide range of RNA modifications including m
MeSH term(s)	Adenosine/metabolism ; Animals ; Atherosclerosis/metabolism ; Cardiovascular Diseases/metabolism ; Cardiovascular Diseases/therapy ; Coronary Disease/metabolism ; Epigenesis, Genetic ; Fibrosis/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Heart Failure/metabolism ; Humans ; Hypertension, Pulmonary/metabolism ; Hypertrophy ; Metabolic Syndrome/metabolism ; Methylation ; Mice ; Microcirculation ; Myocardium/metabolism ; RNA/metabolism ; RNA Processing, Post-Transcriptional ; RNA, Untranslated/metabolism ; Regeneration ; Reperfusion Injury ; Transcriptome
Chemical Substances	RNA, Untranslated ; RNA (63231-63-0) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2021-05-06
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2021.119565
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Article ; Online: Effect of tannic acid modification on the interface and emulsification properties of zein colloidal particles

Li, Bojia / Wang, Xinyi / Gao, Jin / Liang, Hongshan / Wu, Di / Chu, Shang / Zhu, Xiangwei / Zhou, Bin

Journal of the Science of Food and Agriculture. 2024 Jan. 30, v. 104, no. 2 p.643-654

2024

Abstract: BACKGROUND: Interface modification driven by supramolecular self‐assembly has been accepted as a valuable strategy for emulsion stabilization enhancement. However, there has been a dearth of comparative research on the effect of simple complexation and ... ...

Abstract	BACKGROUND: Interface modification driven by supramolecular self‐assembly has been accepted as a valuable strategy for emulsion stabilization enhancement. However, there has been a dearth of comparative research on the effect of simple complexation and assembly from the perspective of the responsible mechanism. RESULTS: The present study selected zein and tannic acid (TA) as representative protein and polyphenol modules for self‐assembly (coined as TA‐modified zein particle and TA‐zein complex particle) to explore the surface properties and interfacial behavior, as well as the stability of constructed Pickering emulsions to obtain the regulation law of different modification methods on the interfacial behavior of colloidal particles. The results demonstrated that TA‐modified zein colloidal particles potentially improved the emulsifying properties. When the TA concentration was 3 mmol L⁻¹, the optimized TA‐modified zein particle was nano‐sized (109.83 nm) and had advantageous interfacial properties, including sharply reduced surface hydrophobicity, as well as a low diffusion rate at the oil/water interface. As a result, the shelf life of Pickering emulsion containing 50% oil phase was extended to 90 days. CONCLUSION: Through multi‐angled research on the properties of the interfacial membrane, improvement of emulsion stability was a result of the formation of viscoelastic interfacial film that resulted from the decrease of absorption rate between particles and interface. Using refined regulation to investigate the role of different sample preparation methods from a mechanistic perspective. Overall, the present study has provided a reference for TA to regulate the surface properties and interface behavior of zein colloidal particles, enriched the understanding of colloidal interface assembly, and provided a theoretical basis for the quality control of interface‐oriented food systems. © 2023 Society of Chemical Industry.
Keywords	absorption ; agriculture ; emulsifying ; emulsions ; hydrophobicity ; oils ; polyphenols ; quality control ; shelf life ; tannins ; viscoelasticity ; zein
Language	English
Dates of publication	2024-0130
Size	p. 643-654.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.12953
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Characteristics and catalytic mechanism of a novel multifunctional oxidase, CpmO, for chloramphenicols degradation from Sphingobium sp. WTD-1.

Gao, Yongsheng / Cheng, Huan / Song, Qinghui / Huang, Junwei / Liu, Junwei / Pan, Dandan / Wu, Xiangwei

Journal of hazardous materials

2023 Volume 465, Page(s) 133348

Abstract: Chloramphenicols (CAPs) are ubiquitous emerging pollutants that threaten ecological environments and human health. Microbial and enzyme-based biodegradation strategies offer a cost-effective environmentally friendly approach for CAPs removal from ... ...

Abstract	Chloramphenicols (CAPs) are ubiquitous emerging pollutants that threaten ecological environments and human health. Microbial and enzyme-based biodegradation strategies offer a cost-effective environmentally friendly approach for CAPs removal from contaminated sites. Here, CpmO, a novel multifunctional oxidase for CAP degradation was identified from the CAP-degrading strain Sphingobium sp. WTD-1. This enzyme was found to be responsible for both the oxidation of the C3-hydroxyl and oxidative cleavage of the C1-C2 bond of CAP, and the oxidative cleavage pathway of CAP was dominant. The catalytic efficiency of CpmO for CAP was 41.6 times that for thiamphenicol (TAP) under the optimal conditions (40 °C, pH 6.0). CpmO was identified as a member of the glucose-methanol-choline oxidoreductase family. Molecular docking and site-directed mutagenesis analysis indicated that CAP was connected to the key amino acid residues E231/E395, K277, and I273/A276 in CpmO through hydrogen bonding, nonclassical hydrogen bonding, and π-π stacking forces, respectively. The catalytic activities of the A276W, K277P, and E231S mutants were found to be 1.1 times, 6.4 times, and 13.2 times higher than that of the wild type, respectively. These findings provide genetic resources and theoretical guidance for future application in biotechnological and metabolic engineering efforts for the remediation of CAPs-contaminated environments.
MeSH term(s)	Humans ; Oxidoreductases ; Binding Sites/genetics ; Molecular Docking Simulation ; Chloramphenicol ; Mutagenesis, Site-Directed
Chemical Substances	Oxidoreductases (EC 1.-) ; Chloramphenicol (66974FR9Q1)
Language	English
Publishing date	2023-12-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2023.133348
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Article ; Online: Serum analysis based on SERS combined with 2D convolutional neural network and Gramian angular field for breast cancer screening.

Cheng, Nuo / Gao, Yan / Ju, Shaowei / Kong, Xiangwei / Lyu, Jiugong / Hou, Lijie / Jin, Lihong / Shen, Bingjun

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2024 Volume 312, Page(s) 124054

Abstract: Breast cancer is a significant cause of death among women worldwide. It is crucial to quickly and accurately diagnose breast cancer in order to reduce mortality rates. While traditional diagnostic techniques for medical imaging and pathology samples have ...

Abstract	Breast cancer is a significant cause of death among women worldwide. It is crucial to quickly and accurately diagnose breast cancer in order to reduce mortality rates. While traditional diagnostic techniques for medical imaging and pathology samples have been commonly used in breast cancer screening, they still have certain limitations. Surface-enhanced Raman spectroscopy (SERS) is a fast, highly sensitive and user-friendly method that is often combined with deep learning techniques like convolutional neural networks. This combination helps identify unique molecular spectral features, also known as "fingerprint", in biological samples such as serum. Ultimately, this approach is able to accurately screen for cancer. The Gramian angular field (GAF) algorithm can convert one-dimensional (1D) time series into two-dimensional (2D) images. These images can be used for data visualization, pattern recognition and machine learning tasks. In this study, 640 serum SERS from breast cancer patients and healthy volunteers were converted into 2D spectral images by Gramian angular field (GAF) technique. These images were then used to train and test a two-dimensional convolutional neural network-GAF (2D-CNN-GAF) model for breast cancer classification. We compared the performance of the 2D-CNN-GAF model with other methods, including one-dimensional convolutional neural network (1D-CNN), support vector machine (SVM), K-nearest neighbor (KNN) and principal component analysis-linear discriminant analysis (PCA-LDA), using various evaluation metrics such as accuracy, precision, sensitivity, F1-score, receiver operating characteristic (ROC) curve and area under curve (AUC) value. The results showed that the 2D-CNN model outperformed the traditional models, achieving an AUC value of 0.9884, an accuracy of 98.13%, sensitivity of 98.65% and specificity of 97.67% for breast cancer classification. In this study, we used conventional nano-silver sol as the SERS-enhanced substrate and a portable laser Raman spectrometer to obtain the serum SERS data. The 2D-CNN-GAF model demonstrated accurate and automatic classification of breast cancer patients and healthy volunteers. The method does not require augmentation and preprocessing of spectral data, simplifying the processing steps of spectral data. This method has great potential for accurate breast cancer screening and also provides a useful reference in more types of cancer classification and automatic screening.
MeSH term(s)	Humans ; Female ; Early Detection of Cancer ; Breast Neoplasms/diagnosis ; Spectrum Analysis, Raman ; Neural Networks, Computer ; Breast
Language	English
Publishing date	2024-02-19
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2024.124054
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