Result 1 - 10 of total 882
Resource: weekblad voor Wageningen UR (2010) ; ISSN: 1874-3625
2010
| Keywords | Life Science |
|---|---|
| Language | Dutch |
| Publishing country | nl |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
International journal of behavioral medicine
2021 Volume 29, Issue 2, Page(s) 160–174
Abstract: Background: Puberty and menstruation are significant stressors for adolescent girls and young women in low-resource settings in sub-Saharan Africa. However, little is known about the impact of these stressors on girls' mental health and reproductive ... ...
| Abstract | Background: Puberty and menstruation are significant stressors for adolescent girls and young women in low-resource settings in sub-Saharan Africa. However, little is known about the impact of these stressors on girls' mental health and reproductive health. Methods: In 2018, a cross-sectional self-report survey was conducted with 581 adolescent girls and young women between 13 to 21 years old who had reached menarche and were attending secondary school in Moshi, Tanzania. Structural equation modeling was used to examine the relationships of puberty-related stressors and menstruation-related stressors (menstrual deficits, movement/activity difficulties, and menstrual symptoms) to depression, anxiety, and the likelihood of reporting reproductive tract infection (RTI) symptoms. Results: Puberty-specific stressors, menstrual symptoms, and menstrual deficits were associated with depression and anxiety. Movement/activity difficulties were associated with anxiety. Increases in menstrual symptoms, menstrual deficits, puberty stressors, and depression were associated with an increased likelihood of reporting a lifetime RTI. However, the relationship of puberty stressors and depression with RTIs was no longer significant in two of three models after correcting for Type I error. Conclusions: Overall, puberty- and menstruation-related stressors were associated with mental health and symptoms of reproductive tract infections. This suggests there is an important relationship between stressors specific to adolescent girls and young women during puberty, mental health, and reproductive health. There is a need for adolescent-tailored interventions to reduce the negative impact of stressors among girls transitioning through puberty in sub-Saharan Africa. |
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| MeSH term(s) | Adolescent ; Adult ; Anxiety/epidemiology ; Cross-Sectional Studies ; Depression/epidemiology ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Menstruation/psychology ; Puberty ; Reproductive Tract Infections ; Surveys and Questionnaires ; Tanzania/epidemiology ; Young Adult |
| Language | English |
| Publishing date | 2021-06-30 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 1187972-5 |
| ISSN | 1532-7558 ; 1070-5503 |
| ISSN (online) | 1532-7558 |
| ISSN | 1070-5503 |
| DOI | 10.1007/s12529-021-10005-1 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 5090: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
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Journal of pharmaceutical and biomedical analysis
2024 Volume 240, Page(s) 115962
Abstract: DO-2 is a highly selective MNNG HOS transforming (MET) inhibitor. This deuterated drug is thought to diminish the formation of the Aldehyde Oxidase 1 inactive metabolite M3. For various reasons, quantification of DO-2 and its metabolites M3 and DO-5 is ... ...
| Abstract | DO-2 is a highly selective MNNG HOS transforming (MET) inhibitor. This deuterated drug is thought to diminish the formation of the Aldehyde Oxidase 1 inactive metabolite M3. For various reasons, quantification of DO-2 and its metabolites M3 and DO-5 is highly relevant. In this study, we present an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method to quantify DO-2, M3 and DO-5. Rolipram served as the internal standard. Aliquots of 25 µL were mixed with 100 µL internal standard consisting of 10 ng/mL rolipram in acetonitrile. Separation of the analytes was achieved on an Acquity UPLC ® HSS T3 column, utilizing gradient elution with water/formic acid and acetonitrile/formic acid at a flow-rate of 0.400 mL/min. Calibration curves were linear in the range of 1.00 - 1000 ng/mL for DO-2 and DO-5, and 2.00 - 2000 ng/mL for M3 in human plasma. The within-run and between-run precisions of DO-2, DO-5 and M3, also at the level of the LLQ, were within 12.1%, while the accuracy ranged from 89.5 to 108.7%. All values for accuracy, within-run and between-run precisions met the criteria set by the Food and Drug Administration. The method was effectively employed in the analysis of samples obtained from a clinical trial. |
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| MeSH term(s) | Humans ; Chromatography, Liquid/methods ; Tandem Mass Spectrometry/methods ; Rolipram ; Acetonitriles ; Reproducibility of Results ; Chromatography, High Pressure Liquid/methods ; Formates |
| Chemical Substances | formic acid (0YIW783RG1) ; Rolipram (K676NL63N7) ; Acetonitriles ; Formates |
| Language | English |
| Publishing date | 2024-01-04 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 604917-5 |
| ISSN | 1873-264X ; 0731-7085 |
| ISSN (online) | 1873-264X |
| ISSN | 0731-7085 |
| DOI | 10.1016/j.jpba.2024.115962 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 1850: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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2023 Volume 18, Issue 7, Page(s) e0284377
Abstract: Many studies have been conducted to produce microbial polyhydroxyalkanoates (PHA), a biopolymer, from Pseudomonas sp. fed with various alkanoic acids. Because this previous data was collected using methodologies that varied in critical aspects, such as ... ...
| Abstract | Many studies have been conducted to produce microbial polyhydroxyalkanoates (PHA), a biopolymer, from Pseudomonas sp. fed with various alkanoic acids. Because this previous data was collected using methodologies that varied in critical aspects, such as culture media and size range of alkanoic acids, it has been difficult to compare the results for a thorough understanding of the relationship between feedstock and PHA production. Therefore, this study utilized consistent culture media with a wide range of alkanoic acids (C7-C14) to produce medium chain length PHAs. Three strains of Pseudomonas putida (NRRL B-14875, KT2440, and GN112) were used, and growth, cell dry weight, PHA titer, monomer distribution, and molecular weights were all examined. It was determined that although all the strains produced similar PHA titers using C7-C9 alkanoic acids, significant differences were observed with the use of longer chain feedstocks. Specifically, KT2440 and its derivative GN112 produced higher PHA titers compared to B-14875 when fed longer chain alkanoates. We also compared several analytical techniques for determining amounts of PHA and found they produced different results. In addition, the use of an internal standard had a higher risk of calculating inaccurate concentrations compared to an external standard. These observations highlight the importance of considering this aspect of analysis when evaluating different studies. |
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| MeSH term(s) | Pseudomonas putida ; Polyhydroxyalkanoates ; Fatty Acids ; Culture Media |
| Chemical Substances | Polyhydroxyalkanoates ; Fatty Acids ; Culture Media |
| Language | English |
| Publishing date | 2023-07-20 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2267670-3 |
| ISSN | 1932-6203 ; 1932-6203 |
| ISSN (online) | 1932-6203 |
| ISSN | 1932-6203 |
| DOI | 10.1371/journal.pone.0284377 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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1993
| Author's details | Jan Sikkema |
|---|---|
| Keywords | Tetralin ; Katalyse ; Mikroorganismus |
| Subject | Tetrahydronaphthalin ; Keim ; Mikrobe ; Mikroben |
| Size | 161 S. : graph. Darst. |
| Publishing country | Netherlands |
| Document type | Book ; Thesis |
| Thesis / German Habilitation thesis | Wageningen, Landbouwuniv., Diss., 1993 |
| Note | Zsfassung in niederländ. Sprache |
| HBZ-ID | HT005001682 |
| ISBN | 90-5485-125-2 ; 978-90-5485-125-7 |
| Database | Catalogue ZB MED Nutrition, Environment, Agriculture |
| Shelf mark | Loan status | Location |
|---|---|---|
| AY 16454 | order for loan | Geschlossenes Magazin (U2) |
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Current protocols
2023 Volume 3, Issue 9, Page(s) e882
Abstract: Golden Gate Assembly is a flexible method of DNA assembly and cloning that permits the joining of multiple fragments in a single reaction through predefined connections. The method depends on cutting DNA using a Type IIS restriction enzyme, which cuts ... ...
| Abstract | Golden Gate Assembly is a flexible method of DNA assembly and cloning that permits the joining of multiple fragments in a single reaction through predefined connections. The method depends on cutting DNA using a Type IIS restriction enzyme, which cuts outside its recognition site and therefore can generate overhangs of any sequence while separating the recognition site from the generated fragment. By choosing compatible fusion sites, Golden Gate permits the joining of multiple DNA fragments in a defined order in a single reaction. Conventionally, this method has been used to join five to eight fragments in a single assembly round, with yield and accuracy dropping off rapidly for more complex assemblies. Recently, we demonstrated the application of comprehensive measurements of ligation fidelity and bias data using data-optimized assembly design (DAD) to enable a high degree of assembly accuracy for very complex assemblies with the simultaneous joining of as many as 52 fragments in one reaction. Here, we describe methods for applying DAD principles and online tools to evaluate the fidelity of existing fusion site sets and assembly standards, selecting new optimal sets, and adding fusion sites to existing assemblies. We further describe the application of DAD to divide known sequences at optimal points, including designing one-pot assemblies of small genomes. Using the T7 bacteriophage genome as an example, we present a protocol that includes removal of native Type IIS sites (domestication) simultaneously with parts generation by PCR. Finally, we present recommended cycling protocols for assemblies of medium to high complexity (12-36 fragments), methods for producing high-quality parts, examples highlighting the importance of DNA purity and fragment stoichiometric balance for optimal assembly outcomes, and methods for assessing assembly success. © 2023 New England Biolabs, Inc. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Assessing the fidelity of an overhang set using the NEBridge Ligase Fidelity Viewer Basic Protocol 2: Generating a high-fidelity overhang set using the NEBridge GetSet Tool Alternate Protocol 1: Expanding an existing overhang set using the NEBridge GetSet Tool Basic Protocol 3: Dividing a genomic sequence with optimal fusion sites using the NEBridge SplitSet Tool Basic Protocol 4: One-pot Golden Gate Assembly of 12 fragments into a destination plasmid Alternate Protocol 2: One-pot Golden Gate Assembly of 24+ fragments into a destination plasmid Basic Protocol 5: One-pot Golden Gate Assembly of the T7 bacteriophage genome from 12+ parts Support Protocol 1: Generation of high-purity amplicons for assembly Support Protocol 2: Cloning assembly parts into a holding vector Support Protocol 3: Quantifying DNA concentration using a Qubit 4 fluorometer Support Protocol 4: Visualizing large assemblies via TapeStation Support Protocol 5: Validating phage genome assemblies via ONT long-read sequencing. |
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| MeSH term(s) | Bacteriophage T7 ; Bacteriophages ; Bicycling ; DNA Restriction Enzymes ; Domestication |
| Chemical Substances | DNA Restriction Enzymes (EC 3.1.21.-) |
| Language | English |
| Publishing date | 2023-09-17 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 2691-1299 |
| ISSN (online) | 2691-1299 |
| DOI | 10.1002/cpz1.882 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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2018 Volume 49, Issue 2, Page(s) 129–132
Abstract: In Masanga, Sierra Leone, a multigravid woman presented with a urine pregnancy test negative molar pregnancy. This can be explained by the 'hook-effect'. In resource-poor settings where quantitative serum hCG cannot be determined, it is of paramount ... ...
| Abstract | In Masanga, Sierra Leone, a multigravid woman presented with a urine pregnancy test negative molar pregnancy. This can be explained by the 'hook-effect'. In resource-poor settings where quantitative serum hCG cannot be determined, it is of paramount importance to remain vigilant of the diagnosis of molar pregnancy. Clinical judgement and sonography remain key in diagnosing molar pregnancy in district hospitals in low- and middle-income countries (LMICs), especially since their occurrence is much more common in these countries. |
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| MeSH term(s) | Adult ; Chorionic Gonadotropin/urine ; False Negative Reactions ; Female ; Humans ; Hydatidiform Mole/diagnosis ; Hydatidiform Mole/epidemiology ; Hydatidiform Mole/pathology ; Hydatidiform Mole/urine ; Pregnancy ; Sierra Leone/epidemiology ; Ultrasonography ; Uterine Neoplasms/diagnosis ; Uterine Neoplasms/epidemiology ; Uterine Neoplasms/pathology ; Uterine Neoplasms/urine |
| Chemical Substances | Chorionic Gonadotropin |
| Language | English |
| Publishing date | 2018-12-07 |
| Publishing country | England |
| Document type | Case Reports ; Journal Article |
| ZDB-ID | 193169-6 |
| ISSN | 1758-1133 ; 0049-4755 |
| ISSN (online) | 1758-1133 |
| ISSN | 0049-4755 |
| DOI | 10.1177/0049475518816222 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 1056: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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Frontiers in molecular biosciences
2022 Volume 9, Page(s) 863470
Abstract: Exposed to changes in their environment, microorganisms will adapt their phenotype, including metabolism, to ensure survival. To understand the adaptation principles, resource allocation-based approaches were successfully applied to predict an optimal ... ...
| Abstract | Exposed to changes in their environment, microorganisms will adapt their phenotype, including metabolism, to ensure survival. To understand the adaptation principles, resource allocation-based approaches were successfully applied to predict an optimal proteome allocation under (quasi) steady-state conditions. Nevertheless, for a general, dynamic environment, enzyme kinetics will have to be taken into account which was not included in the linear resource allocation models. To this end, a resource-dependent kinetic model was developed and applied to the model organism |
|---|---|
| Language | English |
| Publishing date | 2022-05-16 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2814330-9 |
| ISSN | 2296-889X |
| ISSN | 2296-889X |
| DOI | 10.3389/fmolb.2022.863470 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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NAR genomics and bioinformatics
2023 Volume 5, Issue 3, Page(s) lqad070
Abstract: Single-cell genomics is now producing an ever-increasing amount of datasets that, when integrated, could provide large-scale reference atlases of tissue in health and disease. Such large-scale atlases increase the scale and generalizability of analyses ... ...
| Abstract | Single-cell genomics is now producing an ever-increasing amount of datasets that, when integrated, could provide large-scale reference atlases of tissue in health and disease. Such large-scale atlases increase the scale and generalizability of analyses and enable combining knowledge generated by individual studies. Specifically, individual studies often differ regarding cell annotation terminology and depth, with different groups specializing in different cell type compartments, often using distinct terminology. Understanding how these distinct sets of annotations are related and complement each other would mark a major step towards a consensus-based cell-type annotation reflecting the latest knowledge in the field. Whereas recent computational techniques, referred to as 'reference mapping' methods, facilitate the usage and expansion of existing reference atlases by mapping new datasets (i.e. queries) onto an atlas; a systematic approach towards harmonizing dataset-specific cell-type terminology and annotation depth is still lacking. Here, we present 'treeArches', a framework to automatically build and extend reference atlases while enriching them with an updatable hierarchy of cell-type annotations across different datasets. We demonstrate various use cases for treeArches, from automatically resolving relations between reference and query cell types to identifying unseen cell types absent in the reference, such as disease-associated cell states. We envision treeArches enabling data-driven construction of consensus atlas-level cell-type hierarchies and facilitating efficient usage of reference atlases. |
|---|---|
| Language | English |
| Publishing date | 2023-07-26 |
| Publishing country | England |
| Document type | Journal Article |
| ISSN | 2631-9268 |
| ISSN (online) | 2631-9268 |
| DOI | 10.1093/nargab/lqad070 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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PLOS global public health
2023 Volume 3, Issue 7, Page(s) e0001544
Abstract: Dysmenorrhea (menstrual pain) is common among adolescent girls globally, but many girls in Sub-Saharan Africa do not receive effective treatment. Qualitative interviews were used to describe adolescent girls' experiences of dysmenorrhea and identify ... ...
| Abstract | Dysmenorrhea (menstrual pain) is common among adolescent girls globally, but many girls in Sub-Saharan Africa do not receive effective treatment. Qualitative interviews were used to describe adolescent girls' experiences of dysmenorrhea and identify sociocultural barriers to dysmenorrhea management in Moshi, Tanzania. From August to November 2018, in-depth interviews were conducted with 10 adolescent girls and 10 adult experts (e.g., teachers, medical providers) who have experience working with girls in Tanzania. Thematic content analysis identified themes related to dysmenorrhea, including descriptions of dysmenorrhea and the impact of dysmenorrhea on well-being, as well as factors influencing the use of pharmacological and behavioral pain management strategies. Potential barriers to dysmenorrhea management were identified. Dysmenorrhea negatively impacted the physical and psychological well-being of girls and hindered girls' ability to participate in school, work, and social events. The most common pain management strategies were resting, drinking hot water, engaging in physical activity, and taking paracetamol. Barriers to dysmenorrhea management included beliefs that medications are harmful to the body or can hinder fertility, limited knowledge about the benefits of hormonal contraceptives to manage menstruation, little continuing education for healthcare providers, and a lack of consistent access to effective medications, medical care, or other supplies necessary for pain management. Medication hesitancy and inconsistent access to effective medication and other menstrual supplies must be addressed to improve girls' ability to manage dysmenorrhea in Tanzania. |
|---|---|
| Language | English |
| Publishing date | 2023-07-06 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 2767-3375 |
| ISSN (online) | 2767-3375 |
| DOI | 10.1371/journal.pgph.0001544 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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