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Article: Bioenergetics of the polyphosphates accumulation in

Rossi, Alejandro / Grumelli, Sandra

2022 Volume 9, Issue 2, Page(s) e12601

Abstract: Pseudomonas ... ...

Abstract	Pseudomonas aeruginosa
Language	English
Publishing date	2022-12-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e12601
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Bioenergetics of the polyphosphates accumulation in Pseudomonas aeruginosa via polyphosphate kinase activation by choline in a lung colonization model

Alejandro Rossi / Sandra Grumelli

Heliyon, Vol 9, Iss 2, Pp e12601- (2023)

2023

Abstract: Pseudomonas aeruginosa is an ubiquitous and opportunistic bacteria found in water, soil, plants, and immunocompromised humans. Cystic fibrosis (CF) patients are the most vulnerable population to lung colonization by these bacteria. Upon infection, ... ...

Abstract	Pseudomonas aeruginosa is an ubiquitous and opportunistic bacteria found in water, soil, plants, and immunocompromised humans. Cystic fibrosis (CF) patients are the most vulnerable population to lung colonization by these bacteria. Upon infection, choline and succinate are released from the CF lungs and are catabolized by P. aeruginosa. The bacteria accumulate inorganic polyphosphates, rather than succinate, when choline is catabolized, producing physiological and morphological changes leading to ineradicable infection. Thus, we sought to quantify the enzymes responsible for polyphosphate accumulation and to determine how choline catabolism affects energy flow and storage. Subcellular fractions showed that exo-polyphosphate phosphatase (PPX) activity resides mainly in the periplasm, and three isoenzymes of 24, 70, and 200 KD were found. The PPX activity in the periplasm of bacteria grown with choline was inhibited in an anti-competitive manner from Km 0.5 to 1 μM, and their Vmax increased from 50 to 100 nmol PO4≡/min/g of protein in succinate medium. Since PPX inhibition by choline did not explain the 3.8-fold increase in polyphosphates, we quantified the polyphosphate kinase activity, and its significant 2.4-fold increase was consistent with the accumulation. Furthermore, intracellular ATP concentration directly correlated with the energetic yield of the carbon source and was significantly higher for succinate, suggesting that the restriction of energy caused by choline catabolism may induce morphological and physiological changes to the swarm form thus facilitating their migration and tissue colonization
Keywords	Pseudomonas aeruginosa ; Rhizosphere ; Lung ; Cystic fibrosis ; Choline ; Succinate ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	572
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Bioenergetics of the polyphosphates accumulation in Pseudomonas aeruginosa via polyphosphate kinase activation by choline in a lung colonization model

Rossi, Alejandro / Grumelli, Sandra

Heliyon. 2023 Feb., v. 9, no. 2 p.e12601-

2023

Abstract	Pseudomonas aeruginosa is an ubiquitous and opportunistic bacteria found in water, soil, plants, and immunocompromised humans. Cystic fibrosis (CF) patients are the most vulnerable population to lung colonization by these bacteria. Upon infection, choline and succinate are released from the CF lungs and are catabolized by P. aeruginosa. The bacteria accumulate inorganic polyphosphates, rather than succinate, when choline is catabolized, producing physiological and morphological changes leading to ineradicable infection. Thus, we sought to quantify the enzymes responsible for polyphosphate accumulation and to determine how choline catabolism affects energy flow and storage. Subcellular fractions showed that exo-polyphosphate phosphatase (PPX) activity resides mainly in the periplasm, and three isoenzymes of 24, 70, and 200 KD were found. The PPX activity in the periplasm of bacteria grown with choline was inhibited in an anti-competitive manner from Km 0.5 to 1 μM, and their Vmax increased from 50 to 100 nmol PO4≡/min/g of protein in succinate medium. Since PPX inhibition by choline did not explain the 3.8-fold increase in polyphosphates, we quantified the polyphosphate kinase activity, and its significant 2.4-fold increase was consistent with the accumulation. Furthermore, intracellular ATP concentration directly correlated with the energetic yield of the carbon source and was significantly higher for succinate, suggesting that the restriction of energy caused by choline catabolism may induce morphological and physiological changes to the swarm form thus facilitating their migration and tissue colonization
Keywords	Pseudomonas aeruginosa ; at-risk population ; carbon ; catabolism ; choline ; cystic fibrosis ; energy flow ; energy metabolism ; isozymes ; lungs ; models ; polyphosphate kinase ; polyphosphates ; soil ; succinic acid ; swarms ; Rhizosphere ; Lung ; Succinate ; Inorganic polyphosphates ; Phosphatase ; Kinase ; ATP ; Kinetic of enzymes
Language	English
Dates of publication	2023-02
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e12601
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The social construction of the PrEP1519 study: conditions of possibility for advances in HIV/AIDS prevention.

Barros, Sandra Garrido de / Brasil, Sandra Assis / Rossi, Thais Regis Aranha

Cadernos de saude publica

2023 Volume 39Suppl 1, Issue Suppl 1, Page(s) e00201621

Abstract: This paper analyzed the genesis of the PrEP1519 study and feasibility conditions for its construction. A qualitative-approach study was conducted using the Bourdieusian sociology framework to reconstruct the dynamics of the social environment where ... ...

Abstract	This paper analyzed the genesis of the PrEP1519 study and feasibility conditions for its construction. A qualitative-approach study was conducted using the Bourdieusian sociology framework to reconstruct the dynamics of the social environment where PrEP1519 emerged during 2015-2018. A document analysis and ten in-depth interviews were carried out to analyze the trajectory of the project. Pre-exposure prophylaxis (PrEP) was introduced in Brazil as a public policy in 2017. The lack of scientific evidence available among the adolescent population led to the development of a demonstrative cohort study, associated with an intervention, aimed at combining the prevention and treatment of sexually transmitted infections at three sites in Brazil. PrEP1519 sought to generate evidence for global use and to help the Brazilian Ministry of Health apply PrEP among adolescents. The articulation of bureaucratic, scientific, and activist stakeholders enabled this study. The feasibility conditions for developing PrEP1519 included a favorable relationship of national organizations with international organizations, the favorable approach that public administrators had at the time towards new technologies and prevention strategies, the researchers' previous experience in studies with the target population or with PrEP, articulation efforts with social movements, civil society organizations, and other public agencies, and the integration between scientific institutions, which allowed using international resources and developing a response to the problem. Completing this study at a moment when conservatism advances in Brazil demands that the scientific community and activists closely monitor and take stances on PrEP to ensure its availability for adolescents as a public policy.
MeSH term(s)	Adolescent ; Humans ; HIV Infections/drug therapy ; Cohort Studies ; Brazil/epidemiology ; Acquired Immunodeficiency Syndrome/drug therapy ; Sexually Transmitted Diseases/epidemiology ; Anti-HIV Agents/therapeutic use
Chemical Substances	Anti-HIV Agents
Language	English
Publishing date	2023-03-27
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	1115730-6
ISSN	1678-4464 ; 0102-311X
ISSN (online)	1678-4464
ISSN	0102-311X
DOI	10.1590/0102-311XEN201621
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Article ; Online: Scavenging of Superoxide in Aprotic Solvents of Four Isoflavones That Mimic Superoxide Dismutase.

Yu, Sandra / Caruso, Francesco / Belli, Stuart / Rossi, Miriam

International journal of molecular sciences

2023 Volume 24, Issue 4

Abstract: Isoflavones are plant-derived natural products commonly found in legumes that show a large spectrum of biomedical activities. A common antidiabetic remedy in traditional Chinese medicine, ...

Abstract	Isoflavones are plant-derived natural products commonly found in legumes that show a large spectrum of biomedical activities. A common antidiabetic remedy in traditional Chinese medicine,
MeSH term(s)	Humans ; Antioxidants/chemistry ; Biological Products/chemistry ; Diabetes Mellitus, Type 2 ; Hydrogen Peroxide ; Isoflavones/chemistry ; PPAR gamma/chemistry ; Superoxide Dismutase/chemistry ; Superoxides/chemistry
Chemical Substances	Antioxidants ; Biological Products ; Hydrogen Peroxide (BBX060AN9V) ; Isoflavones ; PPAR gamma ; Superoxide Dismutase (EC 1.15.1.1) ; Superoxides (11062-77-4) ; formononetin (295DQC67BJ)
Language	English
Publishing date	2023-02-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24043815
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Article ; Online: Metalloenzyme Inhibitors against Zoonotic Infections: Focus on

Rossi, Sara / Tudino, Valeria / Carullo, Gabriele / Butini, Stefania / Campiani, Giuseppe / Gemma, Sandra

ACS infectious diseases

2024

Abstract: The term "zoonosis" denotes diseases transmissible among vertebrate animals and humans. These diseases constitute a significant public health challenge, comprising 61% of human pathogens and causing an estimated 2.7 million deaths annually. Zoonoses not ... ...

Abstract	The term "zoonosis" denotes diseases transmissible among vertebrate animals and humans. These diseases constitute a significant public health challenge, comprising 61% of human pathogens and causing an estimated 2.7 million deaths annually. Zoonoses not only affect human health but also impact animal welfare and economic stability, particularly in low- and middle-income nations. Leishmaniasis and schistosomiasis are two important neglected tropical diseases with a high prevalence in tropical and subtropical areas, imposing significant burdens on affected regions. Schistosomiasis, particularly rampant in sub-Saharan Africa, lacks alternative treatments to praziquantel, prompting concerns regarding parasite resistance. Similarly, leishmaniasis poses challenges with unsatisfactory treatments, urging the development of novel therapeutic strategies. Effective prevention demands a One Health approach, integrating diverse disciplines to enhance diagnostics and develop safer drugs. Metalloenzymes, involved in parasite biology and critical in different biological pathways, emerged in the last few years as useful drug targets for the treatment of human diseases. Herein we have reviewed recent reports on the discovery of inhibitors of metalloenzymes associated with zoonotic diseases like histone deacetylases (HDACs), carbonic anhydrase (CA), arginase, and heme-dependent enzymes.
Language	English
Publishing date	2024-04-26
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2373-8227
ISSN (online)	2373-8227
DOI	10.1021/acsinfecdis.4c00163
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Personal qualities needed by undergraduate nursing students for a successful work integrated learning (WIL) experience.

Walker, Sandra B / Rossi, Dolene M

Nurse education today

2021 Volume 102, Page(s) 104936

Abstract: Background: International research has shown that during work integrated learning (WIL) undergraduate nursing students may experience stress, self-doubt, and coping issues, however personal qualities these students bring to their WIL journey or those ... ...

Abstract	Background: International research has shown that during work integrated learning (WIL) undergraduate nursing students may experience stress, self-doubt, and coping issues, however personal qualities these students bring to their WIL journey or those needed to succeed during WIL remain unknown. Objective: To describe the views of students midway through their Bachelor of Nursing program regarding personal qualities they bring to and need for WIL success. Design: This article reports on a descriptive study that used an electronic questionnaire to collect the data. Settings and participants: Bachelor of nursing students (n = 421), who were studying fulltime or part time equivalent, at a regional university in Queensland Australia, were invited to complete the anonymous electronic questionnaire that was developed by the research team following an extensive review of literature. Inclusion criteria required participants to have completed at least one WIL experience and be midway through their three-year Bachelor of Nursing program. Methods: Data were collected during January and February 2017. The electronic questionnaire consisted of demographic, forced choice questions, and open-ended questions. This paper presents the qualitative analysis of responses to two of the open-ended questions that focused on personal strengths and work integrated learning experiences. Data were analysed using the inductive content analysis approach. Results: Six personal qualities were identified including: enthusiasm to succeed, commitment to learning, self-motivation, confidence, effective communication skills, and compassion. Conclusions: Pre-entry screening of applicants to bachelor level nursing programs and the delivery of learning opportunities that encourages both reflection and debriefing may assist in describing personal qualities needed to succeed in WIL.
MeSH term(s)	Australia ; Education, Nursing, Baccalaureate ; Humans ; Learning ; Qualitative Research ; Queensland ; Students, Nursing
Language	English
Publishing date	2021-04-26
Publishing country	Scotland
Document type	Journal Article
ZDB-ID	1062570-7
ISSN	1532-2793 ; 0260-6917
ISSN (online)	1532-2793
ISSN	0260-6917
DOI	10.1016/j.nedt.2021.104936
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3117: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: The social construction of the PrEP1519 study

Sandra Garrido de Barros / Sandra Assis Brasil / Thais Regis Aranha Rossi

Cadernos de Saúde Pública, Vol 39, Iss suppl

conditions of possibility for advances in HIV/AIDS prevention

2023 Volume 1

Abstract	This paper analyzed the genesis of the PrEP1519 study and feasibility conditions for its construction. A qualitative-approach study was conducted using the Bourdieusian sociology framework to reconstruct the dynamics of the social environment where PrEP1519 emerged during 2015-2018. A document analysis and ten in-depth interviews were carried out to analyze the trajectory of the project. Pre-exposure prophylaxis (PrEP) was introduced in Brazil as a public policy in 2017. The lack of scientific evidence available among the adolescent population led to the development of a demonstrative cohort study, associated with an intervention, aimed at combining the prevention and treatment of sexually transmitted infections at three sites in Brazil. PrEP1519 sought to generate evidence for global use and to help the Brazilian Ministry of Health apply PrEP among adolescents. The articulation of bureaucratic, scientific, and activist stakeholders enabled this study. The feasibility conditions for developing PrEP1519 included a favorable relationship of national organizations with international organizations, the favorable approach that public administrators had at the time towards new technologies and prevention strategies, the researchers’ previous experience in studies with the target population or with PrEP, articulation efforts with social movements, civil society organizations, and other public agencies, and the integration between scientific institutions, which allowed using international resources and developing a response to the problem. Completing this study at a moment when conservatism advances in Brazil demands that the scientific community and activists closely monitor and take stances on PrEP to ensure its availability for adolescents as a public policy.
Keywords	Pre-Exposure Prophylaxis ; Teenagers ; Acquired Immunodeficiency Syndrome ; Public Health Policies ; Medical Sociology ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Subject code	300
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Difficult Respiratory Weaning after Cardiac Surgery: A Narrative Review.

Nicolotti, Davide / Grossi, Silvia / Nicolini, Francesco / Gallingani, Alan / Rossi, Sandra

Journal of clinical medicine

2023 Volume 12, Issue 2

Abstract: Respiratory weaning after cardiac surgery can be difficult or prolonged in up to 22.7% of patients. The inability to wean from a ventilator within the first 48 h after surgery is related to increased short- and long-term morbidity and mortality. Risk ... ...

Abstract	Respiratory weaning after cardiac surgery can be difficult or prolonged in up to 22.7% of patients. The inability to wean from a ventilator within the first 48 h after surgery is related to increased short- and long-term morbidity and mortality. Risk factors are mainly non-modifiable and include preoperative renal failure, New York Heart Association, and Canadian Cardiac Society classes as well as surgery and cardio-pulmonary bypass time. The positive effects of pressure ventilation on the cardiovascular system progressively fade during the progression of weaning, possibly leading to pulmonary oedema and failure of spontaneous breathing trials. To prevent this scenario, some parameters such as pulmonary artery occlusion pressure, echography-assessed diastolic function, brain-derived natriuretic peptide, and extravascular lung water can be monitored during weaning to early detect hemodynamic decompensation. Tracheostomy is considered for patients with difficult and prolonged weaning. In such cases, optimal patient selection, timing, and technique may be important to try to reduce morbidity and mortality in this high-risk population.
Language	English
Publishing date	2023-01-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12020497
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Article ; Online: Endocannabinoid System Components of the Female Mouse Reproductive Tract Are Modulated during Reproductive Aging.

Rossi, Gianna / Di Nisio, Valentina / Chiominto, Alessandro / Cecconi, Sandra / Maccarrone, Mauro

International journal of molecular sciences

2023 Volume 24, Issue 8

Abstract: The endocannabinoid (eCB) system has gained ground as a key modulator of several female fertility-related processes, under physiological/pathological conditions. Nevertheless, its modulation during reproductive aging remains unclear. This study aimed to ... ...

Abstract	The endocannabinoid (eCB) system has gained ground as a key modulator of several female fertility-related processes, under physiological/pathological conditions. Nevertheless, its modulation during reproductive aging remains unclear. This study aimed to investigate the expression levels of the main receptors (cannabinoid receptor 1,CB
MeSH term(s)	Mice ; Animals ; Female ; Endocannabinoids/metabolism ; Phospholipase D/metabolism ; Uterus/metabolism ; Reproduction ; Receptors, Cannabinoid ; Receptor, Cannabinoid, CB1 ; Amidohydrolases/metabolism
Chemical Substances	Endocannabinoids ; Phospholipase D (EC 3.1.4.4) ; Receptors, Cannabinoid ; Receptor, Cannabinoid, CB1 ; Amidohydrolases (EC 3.5.-) ; GPR55 protein, mouse
Language	English
Publishing date	2023-04-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24087542
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