Article ; Online: Characterization of 3D embryonic C57BL/6 and A/J mouse midbrain micromass in vitro culture systems for developmental neurotoxicity testing.
Toxicology in vitro : an international journal published in association with BIBRA
2017 Volume 48, Page(s) 33–44
Abstract: ... to facilitate gene x environment considerations. Gestational day (GD) 11 C57BL/6 or GD 12 A/J mouse midbrain ... in both C57BL/6 and A/J cultures. Protein expression showed time-dependent proliferation markers (PCNA ...
| Abstract | In vitro micromass culture systems have been proposed as an alternative method for developmental toxicity assessment to reduce the need for resource-intensive in vivo toxicity testing. In this study, a three-dimensional in vitro embryonic mouse midbrain culture system is characterized in two mouse strains to facilitate gene x environment considerations. Gestational day (GD) 11 C57BL/6 or GD 12 A/J mouse midbrain cells were isolated and cultured in high-density micromass format for 22days in vitro (DIV). Hematoxylin intensity and protein content revealed that neuronal differentiation increases linearly over time in both C57BL/6 and A/J cultures. Protein expression showed time-dependent proliferation markers (PCNA) increased significantly between DIV 4-6 compared to DIV 1. Early and late differentiation markers (e.g. β-tubulin III and NMDAɛ1) were expressed between DIV 6-8 and DIV 8-15, respectively. Immunohistochemistry and protein expression results for proliferation and differentiation markers were concordant. Protein expression patterns for the two mouse strain micromass systems were similar. This study characterizes a novel method for investigating early neurogenesis and may be used to characterize neurodevelopmental toxicity in vitro. Our findings show how the use of different mouse strains in neurodevelopmental studies may extend test systems for gene and environment interaction studies. |
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| MeSH term(s) | Animals ; Cell Differentiation/drug effects ; Cells, Cultured ; Female ; Immunohistochemistry ; Mesencephalon/embryology ; Mesencephalon/pathology ; Mice ; Mice, Inbred A ; Mice, Inbred C57BL ; Neurogenesis/drug effects ; Neurotoxicity Syndromes/pathology ; Pregnancy ; Proliferating Cell Nuclear Antigen/biosynthesis ; Tissue Culture Techniques ; Toxicity Tests/methods |
| Chemical Substances | Proliferating Cell Nuclear Antigen |
| Language | English |
| Publishing date | 2017-12-18 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 639064-x |
| ISSN | 1879-3177 ; 0887-2333 |
| ISSN (online) | 1879-3177 |
| ISSN | 0887-2333 |
| DOI | 10.1016/j.tiv.2017.12.009 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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