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  1. Article ; Online: Correction: Qi, W

    Qi, Wanchen / Lu, Changpeng / Huang, Huiliang / Zhang, Weinan / Song, Shaofei / Liu, Bing

    International journal of molecular sciences

    2020  Volume 21, Issue 8

    Abstract: The authors wish to make the following corrections to this paper [1]:[ ... ]. ...

    Abstract The authors wish to make the following corrections to this paper [1]:[...].
    Language English
    Publishing date 2020-04-21
    Publishing country Switzerland
    Document type Journal Article ; Published Erratum
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms21082915
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Jin-Gui-Shen-Qi Wan alleviates fibrosis in mouse diabetic nephropathy via MHC class II.

    Liang, Dan / Liu, Lu / Qi, Yulin / Nan, Feng / Huang, Ju / Tang, Shiyun / Tang, Jianyuan / Chen, Nianzhi

    Journal of ethnopharmacology

    2024  Volume 324, Page(s) 117745

    Abstract: Ethnopharmacological relevance: Jin-Gui-Shen-Qi Wan (JGSQW) is a traditional Chinese medicine ...

    Abstract Ethnopharmacological relevance: Jin-Gui-Shen-Qi Wan (JGSQW) is a traditional Chinese medicine formula that has been traditionally used to alleviate urinary system ailments such as frequent urination and polyuria. Clinical studies have indicated that when combined with hypoglycaemic drugs, JGSQW exhibits a synergistic effect and can improve diabetic nephropathy (DN), yet its underlying mechanism and targets remain unclear.
    Aim of the study: This study aims to investigate the therapeutic efficacy of JGSQW and its underlying mechanisms using a DN db/db mouse model.
    Materials and methods: Ultrahigh-performance liquid chromatography coupled with mass spectrometry was utilized to analyse the primary active compounds, blood levels, and pharmacokinetics of JGSQW. Additionally, the therapeutic effects of JGSQW and metformin on blood glucose levels, lipid levels, renal function, and renal pathology in diabetic nephropathy mice were investigated using a db/db mouse model. Proteomic analysis was carried out to identify the primary target of JGSQW in treating DN. The mechanism of action was verified by western blotting, immunohistochemistry, and immunofluorescence. Then, molecular docking and molecular dynamics, transfection, drug affinity responsive target stability (DARTS) assay and cell thermal migration assay (CETSA) further validated the targeted binding effect.
    Results: JGSQW combined with metformin significantly improved the blood glucose levels, blood lipids, renal function, and renal pathology of DN mice. JGSQW mainly exerted its therapeutic effect on DN by targeting major histocompatibility complex class II (MHC class II) molecules. Immunohistochemistry results showed that JGSQW inhibited the expression of collagen I, fibronectin, and alpha smooth muscle actin (α-SMA) expression. Immunofluorescence and Western blot results showed that JGSQW inhibited the expression of H2-Ab1 and H2-Aa, which are MHC class II molecules, thereby suppressing CD4
    Conclusions: JGSQW combined with metformin may have a synergistic effect to alleviates renal fibrosis in diabetic nephropathy by downregulating immune complex MHC class II molecules and attenuating the antigen presentation effect of MHC class II on CD4.
    MeSH term(s) Mice ; Animals ; Diabetic Nephropathies/pathology ; Blood Glucose ; Molecular Docking Simulation ; Proteomics ; Signal Transduction ; Fibrosis ; Histocompatibility Antigens Class II/pharmacology ; Histocompatibility Antigens Class II/therapeutic use ; Metformin/pharmacology ; Metformin/therapeutic use ; Diabetes Mellitus ; Glucosides ; Monoterpenes
    Chemical Substances peoniflorin (21AIQ4EV64) ; Blood Glucose ; Histocompatibility Antigens Class II ; Metformin (9100L32L2N) ; Glucosides ; Monoterpenes
    Language English
    Publishing date 2024-01-14
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117745
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Shen Qi Wan attenuates renal interstitial fibrosis through upregulating AQP1.

    Lin, Yiyou / Wei, Jiale / Zhang, Yehui / Huang, Junhao / Wang, Sichen / Luo, Qihan / Yu, Hongxia / Ji, Liting / Zhou, Xiaojie / Li, Changyu

    Chinese journal of natural medicines

    2023  Volume 21, Issue 5, Page(s) 359–370

    Abstract: ... to the end-stage renal failure. However, the underlying mechanism of Shen Qi Wan (SQW) on RIF is not fully understood ...

    Abstract Renal interstitial fibrosis (RIF) is the crucial pathway in chronic kidney disease (CKD) leading to the end-stage renal failure. However, the underlying mechanism of Shen Qi Wan (SQW) on RIF is not fully understood. In the current study, we investigated the role of Aquaporin 1 (AQP1) in SQW on tubular epithelial-to-mesenchymal transition (EMT). A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo. Subsequently, the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown. The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine, increased the protein expression of E-cadherin and AQP1 expression, and decreased the expression of vimentin and α-smooth muscle actin (α-SMA). Similarly, treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells. The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1. AQP1 knockdown also increased the mRNA expression of vimentin and α-SMA, and decreased the expression of E-cadherin. The protein expression of vimentin increased, while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells. These results revealed that AQP1 knockdown promoted EMT. Furthermore, AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells. In sum, SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.
    MeSH term(s) Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/pharmacology ; Humans ; Animals ; Mice ; Male ; Cell Line ; Rats ; Kidney/pathology ; Kidney/physiology ; Fibrosis/drug therapy ; Renal Insufficiency, Chronic/drug therapy ; Adenine ; Epithelial-Mesenchymal Transition ; Aquaporin 1/metabolism
    Chemical Substances Drugs, Chinese Herbal ; Adenine (JAC85A2161) ; Aqp1 protein, mouse ; Aquaporin 1 (146410-94-8)
    Language English
    Publishing date 2023-05-26
    Publishing country China
    Document type Journal Article
    ZDB-ID 2192577-X
    ISSN 1875-5364 ; 2095-6975 ; 1672-3651
    ISSN (online) 1875-5364
    ISSN 2095-6975 ; 1672-3651
    DOI 10.1016/S1875-5364(23)60453-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Qi, return to bodily experience

    Xinzhe Huang

    Chinese Medicine and Culture, Vol 3, Iss 4, Pp 216-

    A new perspective of Qi and Qigong experience research

    2020  Volume 219

    Abstract: For a long period, Qi and Qigong experience were studied within the theoretical framework ... of natural science, psychology, and TCM. This article reviews the modes and problems of Qi and Qigong researches ... these methodologies and ideas are analyzed out as well. Aims to open up a brand-new horizon of Qi and Qigong research ...

    Abstract For a long period, Qi and Qigong experience were studied within the theoretical framework of natural science, psychology, and TCM. This article reviews the modes and problems of Qi and Qigong researches, discusses the theoretical basis of phenomenological researches in anthropology, exemplifies some important researches and ideas about bodily experience. Furthermore, the possibility and difficulty of utilizing these methodologies and ideas are analyzed out as well. Aims to open up a brand-new horizon of Qi and Qigong research, enriches our understandings of the culture of Qi and Qigong, and makes Qigong better adapt itself to the contemporary and the world.
    Keywords body ; embodiment ; phenomenology ; qi ; qigong experience ; Other systems of medicine ; RZ201-999
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Wolters Kluwer Health/LWW
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota.

    Tuohongerbieke, Amanguli / Wang, Huaiyou / Wu, Jiahui / Wang, Zhengqi / Dong, Tingxia / Huang, Yamiao / Zhu, Dequan / Sun, Dongmei / Tsim, Karl Wah Keung

    Pharmaceuticals (Basel, Switzerland)

    2024  Volume 17, Issue 2

    Abstract: Xiao Cheng Qi (XCQ) decoction, an ancient Chinese herbal mixture, has been used in treating slow ...

    Abstract Xiao Cheng Qi (XCQ) decoction, an ancient Chinese herbal mixture, has been used in treating slow-transit constipation (STC) for years. The underlying action mechanism in relieving the clinical symptoms is unclear. Several lines of evidence point to a strong link between constipation and gut microbiota. Short-chain fatty acids (SCFAs) and microbial metabolites have been shown to affect 5-HT synthesis by activating the GPR43 receptor localized on intestinal enterochromaffin cells, since 5-HT receptors are known to influence colonic peristalsis. The objective of this study was to evaluate the efficacy of XCQ in alleviating clinical symptoms in a mouse model of STC induced by loperamide. The application of loperamide leads to a decrease in intestinal transport and fecal water, which is used to establish the animal model of STC. In addition, the relationship between constipation and gut microbiota was determined. The herbal materials, composed of Rhei Radix et Rhizoma (Rhizomes of
    Language English
    Publishing date 2024-01-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph17020153
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Ba-Qi-Rougan formula alleviates hepatic fibrosis by suppressing hepatic stellate cell activation via the MSMP/CCR2/PI3K pathway.

    Xue, Yan / Zhu, Wanchun / Qiao, Fengjie / Yang, Yilan / Qiu, Jiaohao / Zou, Chen / Gao, Yating / Zhang, Xin / Li, Man / Shang, Zhi / Gao, Yueqiu / Huang, Lingying

    Journal of ethnopharmacology

    2024  Volume 329, Page(s) 118169

    Abstract: Ethnopharmacological relevance: The Ba-Qi-Rougan formula (BQRGF) is a traditional and effective ...

    Abstract Ethnopharmacological relevance: The Ba-Qi-Rougan formula (BQRGF) is a traditional and effective compound prescription from Traditional Chinese Medicine (TCM) utilized in treating hepatic fibrosis (HF).
    Aim of the study: We aimed to evaluate the therapeutic efficacy of BQRGF on HF and explore the underlying mechanisms of action.
    Materials and methods: UPLC-Q-TOF-MS technology was employed to identify the material basis of BQRGF. Mice with carbon tetrachloride (CCl
    Results: A total of 48 compounds were identified from BQRGF, with 12 compounds being absorbed into the bloodstream and 9 compounds being absorbed into the liver. Four weeks of BQRGF treatment in the HF mouse model led to significant improvements in biochemical and molecular assays and histopathology, particularly in the medium and high-dose groups. These improvements included a reduction in the level of liver injury and fibrosis-related factors. MSMP levels were elevated in human and mouse fibrotic liver tissues, and this increase was mitigated in HF mice treated with BQRGF. Moreover, primary cells and co-culture studies revealed that BQRGF reduced MSMP expression, decreased the expression of the hepatic stellate cell (HSC) activation markers, and suppressed critical phosphorylated protein levels in the CCR2/PI3K/AKT pathway. These findings were further validated using CCR2/PI3K/AKT signaling inhibitors and agonists in MSMP-activated LX2 cells.
    Conclusions: Collectively, our results suggest that BQRGF combats HF by diminishing MSMP levels and inhibiting MSMP-induced HSC activation through the CCR2/PI3K/AKT pathway.
    Language English
    Publishing date 2024-04-16
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.118169
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation.

    Liu, Yiming / Hong, Xiao / Liu, Liu / Li, Xinyue / Huang, Shuo / Luo, Qihan / Huang, Qiaoyan / Qiu, Jiang / Qiu, Ping / Li, Changyu

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2023  Volume 170, Page(s) 116027

    Abstract: Shen Qi Wan (SQW) has been proven to exert anti-inflammatory effects in the kidneys of CKD models ...

    Abstract Shen Qi Wan (SQW) has been proven to exert anti-inflammatory effects in the kidneys of CKD models accompanied by unclear therapeutic mechanisms. This study aims to evaluate the kidney-protective and anti-inflammatory effects of SQW and to elucidate its fundamental mechanisms for CKD treatment. Firstly, the main active components of SQW were identified by UPLC-Q-TOF/MS technique. Subsequently, we evaluated inflammatory factors, renal function and renal pathology changes following SQW treatment utilizing adenine-induced CKD mice and aquaporin 1 knockout (AQP1
    MeSH term(s) Humans ; Mice ; Animals ; Aquaporin 1/genetics ; Renal Insufficiency, Chronic/drug therapy ; Renal Insufficiency, Chronic/pathology ; Kidney/pathology ; Nephritis/pathology ; Anti-Inflammatory Agents ; beta-Defensins
    Chemical Substances Aquaporin 1 (146410-94-8) ; Anti-Inflammatory Agents ; DEFB1 protein, human ; beta-Defensins ; AQP1 protein, human
    Language English
    Publishing date 2023-12-18
    Publishing country France
    Document type Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2023.116027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Adjuvant qi-invigorating blood-activating tongmai decoction promotes effect of rosuvastatin on senile diabetes mellitus complicated with atherosclerosis.

    Shi, Gang / Yu, Dan / Wu, Juan / Liu, Yanru / Huang, Ruizhen / Zhang, Chengshun

    American journal of translational research

    2023  Volume 15, Issue 5, Page(s) 3433–3441

    Abstract: Objective: This study aimed to explore the efficacy and safety of qi-invigorating blood-activating ... alone were divided into a Monotherapy group, and 65 patients treated with qi-invigorating blood ... than the Monotherapy group (P<0.05).: Conclusion: Qi-invigorating blood-activating tongmai decoction can promote ...

    Abstract Objective: This study aimed to explore the efficacy and safety of qi-invigorating blood-activating tongmai decoction combined with rosuvastatin in the treatment of senile type 2 diabetes mellitus (T2DM) complicated with atherosclerosis (AS).
    Methods: The clinical data of 122 elderly patients with T2DM complicated with AS treated in Hospital of Chengdu University of Traditional Chinese Medicine from February 2020 to November 2021 were retrospectively analyzed. Among them, 57 patients treated with rosuvastatin alone were divided into a Monotherapy group, and 65 patients treated with qi-invigorating blood-activating tongmai decoction adjuvant combined with rosuvastatin were divided into a combined group. The two groups were compared in terms of efficacy after treatment, incidence of adverse reactions after 8 weeks of treatment, and carotid plaque indexes, glucose metabolism indexes and lipid metabolism indexes before and after 8 weeks of treatment.
    Results: The Combined group showed a notably higher response rate than the Monotherapy group (P<0.05), but the two groups showed no significant difference in the incidence of adverse reactions (P>0.05). After 8 weeks of treatment, the intima-media thickness (IMT), plaque area, fasting blood glucose, glycosylated hemoglobin (HbA1c), total cholesterol (TC), triacylglycerol (TG) and low-density lipoprotein-cholesterol (LDL-C) in the two groups decreased significantly, and high-density lipoprotein-cholesterol (HDL-C) in them increased significantly. Furthermore, the Combined group showed significantly higher levels of IMT, plaque area, fasting blood glucose, HbA1c, TC, TG and LDL-C, and a significantly lower HDL-C level than the Monotherapy group (P<0.05).
    Conclusion: Qi-invigorating blood-activating tongmai decoction can promote the therapeutic efficacy of rosuvastatin in elderly patients with T2DM complicated with AS.
    Language English
    Publishing date 2023-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Combining lipidomics and efficacy-oriented compatibility revealed that Qi Ge decoction compatibility improved lipid metabolism in hyperlipidemic rats.

    Fan, Simin / Yu, Xiaoqing / Li, Yanfang / Zhou, Zunming / Ye, Jintong / Guo, Kaixin / Huang, Keer / Ke, Xuehong

    Biomedical chromatography : BMC

    2023  Volume 37, Issue 5, Page(s) e5595

    Abstract: ... compatibility mechanisms of Qi Ge decoction (QG) for improving lipid metabolism in hyperlipidemic rats. The QG ...

    Abstract The mechanism underlying traditional Chinese medicine (TCM) compatibility is difficult to understand. This study combined lipidomics and efficacy-oriented compatibility to explore underlying compatibility mechanisms of Qi Ge decoction (QG) for improving lipid metabolism in hyperlipidemic rats. The QG was divided into three groups according to the efficacy group strategy: the Huangqi-Gegen (HG), Chenpi (CP), and QG groups. Hyperlipidemic rats were treated with QG, HG, CP, or atorvastatin for 3 weeks. The mass spectral data of widely targeted lipidomics were used to evaluate lipid changes. Principal component analysis and orthogonal partial least squares discriminant analysis were used to assess the lipidomic differences between the groups. MetaboAnalyst 5.0 was used to explore metabolic pathways. Compared with the model group, serum cholesterol, triglyceride, and hepatic steatosis were significantly reduced by QG, whereas HG and CP had no significant effects on these indexes. Lipidomics showed that QG, HG, and CP back-regulated 60, 11, and 14 lipids, respectively. Compared with HG and CP, QG had more metabolic targets in diglycerides, triglycerides, ceramides, and phosphatidylethanolamines. Pathway analysis indicated that QG mainly regulated glycerophospholipid and glycerolipid metabolism. This study provided a new method of combining lipidomics and efficacy-oriented compatibility for exploring the scientific connotation of TCM compatibility.
    MeSH term(s) Rats ; Animals ; Lipidomics ; Rats, Sprague-Dawley ; Qi ; Lipid Metabolism ; Drugs, Chinese Herbal/pharmacology ; Medicine, Chinese Traditional
    Chemical Substances Drugs, Chinese Herbal
    Language English
    Publishing date 2023-02-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 632848-9
    ISSN 1099-0801 ; 0269-3879
    ISSN (online) 1099-0801
    ISSN 0269-3879
    DOI 10.1002/bmc.5595
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Shen Qi Wan attenuates renal interstitial fibrosis through upregulating AQP1

    LIN, Yiyou / WEI, Jiale / ZHANG, Yehui / HUANG, Junhao / WANG, Sichen / LUO, Qihan / YU, Hongxia / JI, Liting / Zhou, Xiaojie / Li, Changyu

    Chinese Journal of Natural Medicines. 2023 May, v. 21, no. 5 p.359-370

    2023  

    Abstract: ... to the end-stage renal failure. However, the underlying mechanism of Shen Qi Wan (SQW) on RIF is not fully understood ...

    Abstract Renal interstitial fibrosis (RIF) is the crucial pathway in chronic kidney disease (CKD) leading to the end-stage renal failure. However, the underlying mechanism of Shen Qi Wan (SQW) on RIF is not fully understood. In the current study, we investigated the role of Aquaporin 1 (AQP1) in SQW on tubular epithelial-to-mesenchymal transition (EMT). A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo. Subsequently, the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown. The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine, increased the protein expression of E-cadherin and AQP1 expression, and decreased the expression of vimentin and α-smooth muscle actin (α-SMA). Similarly, treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells. The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1. AQP1 knockdown also increased the mRNA expression of vimentin and α-SMA, and decreased the expression of E-cadherin. The protein expression of vimentin increased, while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells. These results revealed that AQP1 knockdown promoted EMT. Furthermore, AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells. In sum, SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.
    Keywords actin ; adenine ; aquaporins ; blood serum ; cadherins ; collagen ; fibrosis ; gene expression ; kidneys ; mice ; models ; muscles ; protective effect ; protein synthesis ; renal failure ; slugs ; vimentin ; Shen Qi Wan ; Chronic kidney disease ; Renal interstitial fibrosis ; Epithelial to mesenchymal transition ; Aquaporin 1
    Language English
    Dates of publication 2023-05
    Size p. 359-370.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 2192577-X
    ISSN 1875-5364 ; 2095-6975 ; 1672-3651
    ISSN (online) 1875-5364
    ISSN 2095-6975 ; 1672-3651
    DOI 10.1016/S1875-5364(23)60453-4
    Database NAL-Catalogue (AGRICOLA)

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