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Article ; Online: Mechanosensitive release of 5-HT from specialized intestinal epithelial cells.

Alcaino, Constanza

Nature reviews. Gastroenterology & hepatology

2022 Volume 20, Issue 1, Page(s) 4

MeSH term(s)	Humans ; Serotonin ; Intestines ; Intestinal Mucosa ; Epithelial Cells
Chemical Substances	Serotonin (333DO1RDJY)
Language	English
Publishing date	2022-11-21
Publishing country	England
Document type	Journal Article ; Comment
ZDB-ID	2493722-8
ISSN	1759-5053 ; 1759-5045
ISSN (online)	1759-5053
ISSN	1759-5045
DOI	10.1038/s41575-022-00712-9
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Article ; Online: XVIIth Little Brain Big Brain: an extraordinary meeting in extraordinary times.

Alcaino, Constanza / Carbone, Florencia / Neckel, Peter H / Tjaden, Naomi E B

Nature reviews. Gastroenterology & hepatology

2023 Volume 20, Issue 4, Page(s) 197–198

Language	English
Publishing date	2023-02-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2493722-8
ISSN	1759-5053 ; 1759-5045
ISSN (online)	1759-5053
ISSN	1759-5045
DOI	10.1038/s41575-023-00747-6
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Article ; Online: Purification and characterization of a novel cold adapted fungal glucoamylase.

Carrasco, Mario / Alcaíno, Jennifer / Cifuentes, Víctor / Baeza, Marcelo

Microbial cell factories

2017 Volume 16, Issue 1, Page(s) 75

Abstract: ... activity. The optimal conditions for enzyme activity were 30 °C and pH 6.0, and the K : Conclusion ... Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and ... starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C ...

Abstract	Background: Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase that degrades starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C. Thus, isolating new amylases with optimal activity at ambient temperature is essential for improving industrial processes. In this report, a glucoamylase secreted by the cold-adapted yeast Tetracladium sp. was isolated and biochemically characterized. Results: The effects of physicochemical parameters on enzyme activity were analyzed, and pH and temperature were found to be key factors modulating the glucoamylase activity. The optimal conditions for enzyme activity were 30 °C and pH 6.0, and the K Conclusion: The glucoamylase secreted by Tetracladium sp. is a novel cold-adapted enzyme and its properties should render this enzyme suitable for use in industrial processes that require cold-active amylases, such as biofuel production.
MeSH term(s)	Adaptation, Physiological ; Antarctic Regions ; Ascomycota/enzymology ; Ascomycota/growth & development ; Ascomycota/metabolism ; Cold Temperature ; Glucan 1,4-alpha-Glucosidase/chemistry ; Glucan 1,4-alpha-Glucosidase/isolation & purification ; Glucan 1,4-alpha-Glucosidase/metabolism ; Glycosylation ; Hydrogen-Ion Concentration ; Kinetics ; Models, Molecular ; Starch/metabolism ; Substrate Specificity
Chemical Substances	Starch (9005-25-8) ; Glucan 1,4-alpha-Glucosidase (EC 3.2.1.3)
Language	English
Publishing date	2017-05-02
Publishing country	England
Document type	Journal Article
ISSN	1475-2859
ISSN (online)	1475-2859
DOI	10.1186/s12934-017-0693-x
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Article: Diversity of benthic marine mollusks of the Strait of Magellan, Chile (Polyplacophora, Gastropoda, Bivalvia): a historical review of natural history.

Aldea, Cristian / Novoa, Leslie / Alcaino, Samuel / Rosenfeld, Sebastián

ZooKeys

2020 Volume 963, Page(s) 1–36

Abstract: An increase in richness of benthic marine mollusks towards high latitudes has been described on the Pacific coast of Chile in recent decades. This considerable increase in diversity occurs specifically at the beginning of the Magellanic Biogeographic ... ...

Abstract	An increase in richness of benthic marine mollusks towards high latitudes has been described on the Pacific coast of Chile in recent decades. This considerable increase in diversity occurs specifically at the beginning of the Magellanic Biogeographic Province. Within this province lies the Strait of Magellan, considered the most important channel because it connects the South Pacific and Atlantic Oceans. These characteristics make it an interesting area for marine research; thus, the Strait of Magellan has historically been the area with the greatest research effort within the province. However, despite efforts there is no comprehensive and updated list of the diversity of mollusks within the Strait of Magellan up to now. This study consisted of a complete bibliographic review of all available literature that included samples of mollusks in the Strait of Magellan. More than 300 articles were reviewed, covering 200 years of scientific knowledge. There were 2579 records belonging to 412 taxa, of which 347 are valid species. Of the total valid species, 44 (~13%) are considered of doubtful presence in the Strait. This work increases the known richness of mollusks of the Strait of Magellan by 228%; it is also the first report that integrates all available diversity studies of the three most speciose classes of benthic mollusks (Gastropoda, Bivalvia and Polyplacophora) from the Strait of Magellan.
Language	English
Publishing date	2020-08-24
Publishing country	Bulgaria
Document type	Journal Article
ZDB-ID	2445640-8
ISSN	1313-2970 ; 1313-2989
ISSN (online)	1313-2970
ISSN	1313-2989
DOI	10.3897/zookeys.963.52234
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Article ; Online: Aneurisma apical en paciente con miocardiopatía hipertrófica.

Alcaino I, Milton / Aguilar P, Juan / Jorquera B, Claudia

Revista medica de Chile

2021 Volume 149, Issue 3, Page(s) 472–475

Abstract: We report a 51-year-old asymptomatic male, with type II diabetes, referred to our outpatient clinic due to ST and T alterations on the precordial leads on the electrocardiogram. The echocardiogram showed apical akinesia and left ventricular hypertrophy. ... ...

Title translation	Apical aneurysm in a patient with hypertrophic cardiomyopathy.
Abstract	We report a 51-year-old asymptomatic male, with type II diabetes, referred to our outpatient clinic due to ST and T alterations on the precordial leads on the electrocardiogram. The echocardiogram showed apical akinesia and left ventricular hypertrophy. There were no angiographic lesions in the coronary angiography. In the left ventriculography, a hyperdynamic left ventricle with suspected left ventricular hypertrophy and an apical aneurysm were found. The cardiac magnetic resonance confirmed those findings, without late gadolinium enhancement. According to the European Cardiology Society Risk Score, the patient had a low sudden death risk. However, this score does not consider the presence of an aneurysm as risk factor for sudden death, but it is considered in the 2017 ACC/AHA Heart Rhythm Society Guidelines, as a major risk factor. Therefore a defibrillator was implanted, and he was discharged on permanent oral anticoagulation.
MeSH term(s)	Cardiomyopathy, Hypertrophic/complications ; Cardiomyopathy, Hypertrophic/diagnostic imaging ; Contrast Media ; Coronary Angiography ; Diabetes Mellitus, Type 2 ; Electrocardiography ; Gadolinium ; Heart Aneurysm/complications ; Heart Aneurysm/diagnostic imaging ; Humans ; Male ; Middle Aged
Chemical Substances	Contrast Media ; Gadolinium (AU0V1LM3JT)
Language	Spanish
Publishing date	2021-09-03
Publishing country	Chile
Document type	Journal Article
ZDB-ID	732136-3
ISSN	0717-6163 ; 0034-9887
ISSN (online)	0717-6163
ISSN	0034-9887
DOI	10.4067/s0034-98872021000300472
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Article: Synthesis of BiOI/Mordenite Composites for Photocatalytic Treatment of Organic Pollutants Present in Agro-Industrial Wastewater.

Gallegos-Alcaíno, Alejandra / Robles-Araya, Nathaly / Avalos, Camila / Alfonso-Alvarez, Alexander / Rodríguez, Carlos A / Valdés, Héctor / Sánchez-Flores, Norma A / Durán-Alvarez, Juan C / Bizarro, Monserrat / Romero-Salguero, Francisco J / Mera, Adriana C

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 7

Abstract: ... 187 °C and 9 h. In addition, the changes applied to the experimental conditions led ...

Abstract	Recently, bismuth oxyiodide (BiOI) is an attractive semiconductor to use in heterogeneous photocatalysis processes. Unfortunately, BiOI individually shows limited photocatalytic efficiency, instability, and a quick recombination of electron/holes. Considering the practical application of this semiconductor, some studies show that synthetic zeolites provide good support for this photocatalyst. This support material permits a better photocatalytic efficiency because it prevents the quick recombination of photogenerated pairs. However, the optimal conditions (time and temperature) to obtain composites (BiOI/ synthetic zeolite) with high photocatalytic efficiency using a coprecipitation-solvothermal growth method have not yet been reported. In this study, a response surface methodology (RSM) based on a central composite design (CCD) was applied to optimize the synthesis conditions of BiOI/mordenite composites. For this purpose, eleven BiOI/mordenite composites were synthesized using a combined coprecipitation-solvothermal method under different time and temperature conditions. The photocatalytic activities of the synthesized composites were evaluated after 20 min of photocatalytic oxidation of caffeic acid, a typical organic pollutant found in agro-industrial wastewater. Moreover, BiOI/mordenite composites with the highest and lowest photocatalytic activity were physically and chemically characterized using nitrogen adsorption isotherms, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and diffuse reflectance spectroscopy (DRS). The optimal synthesis conditions prove to be 187 °C and 9 h. In addition, the changes applied to the experimental conditions led to surface property modifications that influenced the photocatalytic degradation efficiency of the BiOI/mordenite composite toward caffeic acid photodegradation.
Language	English
Publishing date	2022-03-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12071161
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Article ; Online: Mechanosensitive pore opening of a prokaryotic voltage-gated sodium channel.

Strege, Peter R / Cowan, Luke M / Alcaino, Constanza / Mazzone, Amelia / Ahern, Christopher A / Milescu, Lorin S / Farrugia, Gianrico / Beyder, Arthur

eLife

2023 Volume 12

Abstract: Voltage-gated ion channels (VGICs) orchestrate electrical activities that drive mechanical functions in contractile tissues such as the heart and gut. In turn, contractions change membrane tension and impact ion channels. VGICs are mechanosensitive, but ... ...

Abstract	Voltage-gated ion channels (VGICs) orchestrate electrical activities that drive mechanical functions in contractile tissues such as the heart and gut. In turn, contractions change membrane tension and impact ion channels. VGICs are mechanosensitive, but the mechanisms of mechanosensitivity remain poorly understood. Here, we leverage the relative simplicity of NaChBac, a prokaryotic voltage-gated sodium channel from
MeSH term(s)	Humans ; Ion Channel Gating/physiology ; HEK293 Cells ; Voltage-Gated Sodium Channels ; Mutagenesis
Chemical Substances	Voltage-Gated Sodium Channels
Language	English
Publishing date	2023-03-13
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.79271
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Article ; Online: Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Carrasco, Mario / Rozas, Juan Manuel / Alcaíno, Jennifer / Cifuentes, Víctor / Baeza, Marcelo

Microbial cell factories

2019 Volume 18, Issue 1, Page(s) 45

Abstract: ... However, the commercially available pectinases are highly active at temperatures approximately 50 °C and poorly active ... at temperatures below 35 °C, which is the reason why there is a constant search for cold-active pectinases ... by extracellular protein extracellular extracts obtained from 12 yeast cultures were assayed in plates at 4 °C ...

Abstract	Background: Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market. To prevent the proliferation of undesired microorganisms, to retain labile and volatile flavor compounds, and to save energy, the current trend is to perform this process at low temperatures. However, the commercially available pectinases are highly active at temperatures approximately 50 °C and poorly active at temperatures below 35 °C, which is the reason why there is a constant search for cold-active pectinases. In preliminary studies, pectinolytic activity was detected in cold-adapted yeasts and yeast-like microorganisms isolated from Antarctica. The aim of the present work was to characterize pectinases secreted by these microorganisms and to express the best candidate in Pichia pastoris. Results: Degradation of pectin by extracellular protein extracellular extracts obtained from 12 yeast cultures were assayed in plates at 4 °C to 37 °C and pH from 5.4 to 7.0, obtaining positive results in samples obtained from Dioszegia sp., Phenoliferia glacialis and Tetracladium sp. An enzyme was purified from Tetracladium sp., analyzed by peptide mass fingerprinting and compared to genome and transcriptome data from the same microorganism. Thus, the encoding gene was identified corresponding to a polygalacturonase-encoding gene. The enzyme was expressed in Pichia pastoris, and the recombinant polygalacturonase displayed higher activity at 15 °C than a mesophilic counterpart. Conclusions: Extracellular pectinase activity was found in three yeast and yeast-like microorganisms from which the highest activity was displayed by Tetracladium sp., and the enzyme was identified as a polygalacturonase. The recombinant polygalacturonase produced in P. pastoris showed high activity at 15 °C, representing an attractive candidate to be applied in clarification processes in the production of fermented beverages and fruit juices.
MeSH term(s)	Antarctic Regions ; Ascomycota/enzymology ; Ascomycota/genetics ; Basidiomycota/enzymology ; Basidiomycota/genetics ; Cold Temperature ; Fermentation ; Pichia/genetics ; Pichia/metabolism ; Polygalacturonase/biosynthesis ; Polygalacturonase/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics
Chemical Substances	Recombinant Proteins ; Polygalacturonase (EC 3.2.1.15)
Language	English
Publishing date	2019-03-07
Publishing country	England
Document type	Journal Article
ISSN	1475-2859
ISSN (online)	1475-2859
DOI	10.1186/s12934-019-1092-2
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Article ; Online: PROBLEMÁTICA DE LAS MUJERES EN EL MERCADO DE TRABAJO, EL ESPÍRITU EMPRENDEDOR EN NUEVOS NEGOCIOS Y RELACIÓN CON SUS VALORES Y CREENCIAS

Luis Irribarren Alcaíno

Revista de Estudios Empresariales. Segunda Época, Vol 1, Iss Número 1/2013, Época 2, Pp 87-

2013 Volume 111

Abstract: ... performance level, comparing to that of male peers, c) the growing of role of women into entrepreneurship ...

Abstract	This research paper goes through some of the issues women face in the labour market, as an entrepreneur in new business ventures, and as the owner of those businesses. It considers the relevant research literature on those topics, difficulties for further development and the main challenges women still face nowadays.The expected deliverables comprise a thorough investigation of those topics, highlighting the latest findings on women’s issues, workplace discrimination in areas such as management, and entrepreneurship, and also adding in research results on women’s performance when they are at the forefront of their business ventures.The areas covered in this research are: economic, since it refers to entrepreneurship equity; educational, since a global revolution is currently under way; labour, due to the existence of discrimination when compared to some of her peers,; ethical- religious-cultural, due to its global impact; psycological, when referring to personal values such as self-esteem, failure, happiness, achievement, independence, self-trust, excellence, resilience, involvement, family responsibility, among others; and lastly biological, when it involves maternity, and family/child/elderly/handicapped care.This research focuses on three issues of interest: a) women and the workplace (going out to work away from home; establishing; generating revenue; performing in a variety of work fields; quitting the paid worker post pursuing the entrepreneurship opportunity); b) elements impeding personal development, profiling, management style, and performance level, comparing to that of male peers, c) the growing of role of women into entrepreneurship, her performance as owner of small businesses, and her contribution to the development of emerging countries, as in the case of Chile.
Keywords	women ; workplace ; labour market ; entrepreneurship ; business owners ; performance ; Social Sciences ; H ; Industries. Land use. Labor ; HD28-9999 ; Economic growth ; development ; planning ; HD72-88
Subject code	650
Language	English
Publishing date	2013-07-01T00:00:00Z
Publisher	Facultad de Ciencias Sociales y Jurídicas, Universidad de Jaén
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Effect of Fish Stock Density on Hormone Genes Expression from Brain and Gastrointestinal Tract of

Álvarez, Claudio A / Santana, Paula A / Cárcamo, Claudia B / Cárdenas, Constanza / Morales-Lange, Byron / Ramírez, Felipe / Valenzuela, Cristian / Boltaña, Sebastián / Alcaíno, Javier / Guzmán, Fanny / Mercado, Luis

Animals : an open access journal from MDPI

2022 Volume 12, Issue 9

Abstract: A variety of long-term stress conditions may exist in fish cultivation, some of which are so severe that fish can no longer reestablish homeostasis. In teleost fish, the brain and gastrointestinal tract integrate signals that include the perception of ... ...

Abstract	A variety of long-term stress conditions may exist in fish cultivation, some of which are so severe that fish can no longer reestablish homeostasis. In teleost fish, the brain and gastrointestinal tract integrate signals that include the perception of stress factors regulating physiological responses, such as social stress by fish population density, where peripheral and central signals, such as peptide hormones, are the main regulators. Therefore, we proposed in this study to analyze the effect of different stock densities (SD) in the gene expression of brain neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP), together with the gastrointestinal peptide hormones leptin (Lep), vasointestinal peptide (VIP), and protachykinin-1 (Prk-1) in
Language	English
Publishing date	2022-05-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12091174
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