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  1. Article: Erklärungsbedarf bei Antibiotika stillen. Interview mit Prof. Ralf Stahlmann, Berlin

    Stahlmann, Ralf

    Ärztliche Praxis: Special

    2004  Volume -, Issue 3, Page(s) 12

    Language German
    Document type Article
    ZDB-ID 1317910-x
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 533/X -Spec.-: Show issues Location:
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  2. Article ; Online: A new cell line based coculture system for skin sensitisation testing in one single assay using T cells, aryl hydrocarbon receptor knockout, and co-inhibitory blockage.

    Sonnenburg, Anna / Stahlmann, Ralf / Kreutz, Reinhold / Peiser, Matthias

    Archives of toxicology

    2023  Volume 97, Issue 6, Page(s) 1677–1689

    Abstract: Established in vitro assays for regulatory testing of skin sensitisation partly suffer from only moderate sensitivity, specificity, and predictivity when testing specific groups of chemicals. This may be due to limited biomarker response in vitro in cell ...

    Abstract Established in vitro assays for regulatory testing of skin sensitisation partly suffer from only moderate sensitivity, specificity, and predictivity when testing specific groups of chemicals. This may be due to limited biomarker response in vitro in cell types that interact as crucial players of in vivo skin sensitisation pathogenesis. Here, we propose a molecular approach to overcome this limitation. In our model, we apply genome editing and blocking of immunoregulatory molecules to increase the range of biomarker modulation by sensitising chemicals. To this end, aryl hydrocarbon receptor (AhR) knockout was done by CRISPR/Cas9 technology in THP-1 cells and combined with Programmed Cell Death-Ligand (PD-L)1 blockade. AhR-knockout THP-1 in coculture with HaCaT keratinocytes showed increased CD54 expression compared to wild type cells after stimulation with 10 µmol/L dinitrochlorobenzene (DNCB) that was further enhanced by anti-PD-L1. After stimulation of AhR-knockout THP-1 with 200 µmol/L mercaptobenzothiazol or 10 µmol/L DNCB, cocultivated Jurkat T cells significantly increased expression of T cell receptor-associated CD3. No such increase was detected after prior treatment of THP-1 with 150 µmol/L of irritant sodium lauryl sulphate. Additionally, higher levels of inflammatory cytokines MIP-3α, MIP-1β, TNF-α, and IL-8 were found in supernatants of enhanced loose-fit co-culture based sensitisation assay (eLCSA) after substance treatment. Hence, eLCSA allowed to discriminate between sensitisers and non-sensitisers. Thus, inhibition of immunoinhibitory pathway signalling by combining AhR knockout and PD-L1 antibody blockage into an assay involving main acting cell types in skin sensitisation may increase sensitivity and specificity of such assays and allow potency derivation.
    MeSH term(s) Biomarkers/metabolism ; Cell Line ; Coculture Techniques ; Dinitrochlorobenzene ; Receptors, Aryl Hydrocarbon/genetics ; THP-1 Cells ; Humans ; Jurkat Cells ; Skin Tests
    Chemical Substances Biomarkers ; Dinitrochlorobenzene ; Receptors, Aryl Hydrocarbon
    Language English
    Publishing date 2023-05-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 124992-7
    ISSN 1432-0738 ; 0340-5761
    ISSN (online) 1432-0738
    ISSN 0340-5761
    DOI 10.1007/s00204-023-03506-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article ; Online: Calculated initial parenteral treatment of bacterial infections: Safety and tolerabilty.

    Stahlmann, Ralf / Lode, Hartmut

    GMS infectious diseases

    2020  Volume 8, Page(s) Doc16

    Abstract: This is the fourth chapter of the guideline "Calculated initial parenteral treatment of bacterial infections in adults - update 2018" in the ... ...

    Abstract This is the fourth chapter of the guideline "Calculated initial parenteral treatment of bacterial infections in adults - update 2018" in the 2
    Language English
    Publishing date 2020-03-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2725110-X
    ISSN 2195-8831 ; 2195-8831
    ISSN (online) 2195-8831
    ISSN 2195-8831
    DOI 10.3205/id000060
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Aryl hydrocarbon receptor knockout and antibody blockade of programmed cell death ligand1 increase co-stimulatory molecules on THP-1 and specific cytokine response of human T cells.

    Sonnenburg, Anna / Stahlmann, Ralf / Kreutz, Reinhold / Peiser, Matthias

    Toxicology in vitro : an international journal published in association with BIBRA

    2022  Volume 86, Page(s) 105502

    Abstract: Skin sensitisation involves activation of dendritic cells which activate T cells and induce their clonal expansion. While the first step is covered by OECD-validated new approach methodologies, the latter is not until now. This may be due to a weak ... ...

    Abstract Skin sensitisation involves activation of dendritic cells which activate T cells and induce their clonal expansion. While the first step is covered by OECD-validated new approach methodologies, the latter is not until now. This may be due to a weak dendritic cell activation in vitro that is not strong enough to mediate activation of T cells. Here, we suppressed two inhibitory pathways to overcome this problem. We blocked the Programmed Cell Death (PD) pathway with anti-PD-L1 antibody and knocked out aryl hydrocarbon receptor (AhR) in THP-1 cells by CRISPR/Cas9 technology. Thereby, we reduced AhR
    MeSH term(s) Humans ; Receptors, Aryl Hydrocarbon/genetics ; Receptors, Aryl Hydrocarbon/metabolism ; Cytokines/metabolism ; Dendritic Cells/metabolism ; Lymphocyte Activation ; Apoptosis ; B7-H1 Antigen/genetics ; B7-H1 Antigen/metabolism
    Chemical Substances Receptors, Aryl Hydrocarbon ; Cytokines ; B7-H1 Antigen
    Language English
    Publishing date 2022-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 639064-x
    ISSN 1879-3177 ; 0887-2333
    ISSN (online) 1879-3177
    ISSN 0887-2333
    DOI 10.1016/j.tiv.2022.105502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2223: Show issues Location:
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  5. Book ; Conference proceedings: Quinolones in pediatrics

    Stahlmann, Ralf

    indications and restrictions ; Beiträge eines wissenschaftlichen Symposiums der Paul-Ehrlich-Gesellschaft für Chemotherapie e.V., 19. Oktober 1995, Berlin

    (Chemotherapie-Journal : Supplement ; 13)

    1996  

    Institution Symposium Quinolones in Pediatrics - Indications and Restrictions
    Paul-Ehrlich-Gesellschaft für Chemotherapie
    Author's details [Symposium "Quinolones in Pediatrics - Indications and Restrictions"]. Organisation und Leitung: Ralf Stahlmann
    Series title Chemotherapie-Journal : Supplement ; 13
    Chemotherapie-Journal
    Chemotherapie-Journal ; Supplement
    Collection Chemotherapie-Journal
    Chemotherapie-Journal ; Supplement
    Keywords Anti-Infective Agents, Quinolone / adverse effects / congresses ; Joint Diseases / chemically induced / congresses ; Joint Diseases / in infancy & childhood
    Language English
    Size 40 S. : Ill., graph. Darst.
    Publisher WVG
    Publishing place Stuttgart
    Document type Book ; Conference proceedings
    HBZ-ID HT007425330
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 3509 -Suppl.13- not available for loan Zeitschriften-Lesesaal

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  6. Article: AMT. Neue und alte Antibiotika. Eine aktuelle Bestandsaufnahme / Zertifizierte Fortbildung

    Stahlmann, Ralf / Lode, Hartmut

    Arzneimitteltherapie

    2021  Volume 39, Issue 1/2, Page(s) 2/11

    Language German
    Document type Article
    ZDB-ID 380334-X
    ISSN 0723-6913
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1854: Show issues Location:
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  7. Book: MRSA

    Lode, Hartmut / Stahlmann, Ralf / Witte, Wolfgang

    Infektionen mit multiresistentem Staphylococcus aureus ; Daten zur Epidemiologie, Diagnostik, Klinik und Therapie

    2010  

    Author's details H. Lode ; R. Stahlmann ; W. Witte
    Keywords MRSA
    Subject Methicillin-resistente Staphylococcus-aureus-Stämme ; Meticillin-resistente Staphylococcus-aureus-Stämme ; Multi-resistente Staphylococcus aureus
    Subject code 616.9297
    Language German
    Size 80 S. : Ill., graph. Darst., Kt., 23 cm, 120 gr.
    Publisher ZETT-Verl
    Publishing place Steinen
    Publishing country Germany
    Document type Book
    Note Literaturangaben
    HBZ-ID HT016546375
    ISBN 978-3-926770-41-7 ; 3-926770-41-4
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    2010 A 5397 order for loan Magazin

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  8. Article ; Online: Medication for COVID-19-an Overview of Approaches Currently Under Study.

    Stahlmann, Ralf / Lode, Hartmut

    Deutsches Arzteblatt international

    2020  Volume 117, Issue 13, Page(s) 213–219

    Abstract: Background: With the worldwide spread of SARS-CoV-2 infection, it is becoming increasingly urgent to develop a vaccine to prevent COVID-19, as well as effective drugs to treat it.: Methods: This article is based on a selective literature search in ... ...

    Abstract Background: With the worldwide spread of SARS-CoV-2 infection, it is becoming increasingly urgent to develop a vaccine to prevent COVID-19, as well as effective drugs to treat it.
    Methods: This article is based on a selective literature search in PubMed and ClinicalTrials.gov, followed by an assessment of the ongoing clinical trials that were revealed by the search.
    Results: A number of substances have been found to prevent the reproduction of SARS-CoV-2 in vitro. These include virustatic agents that have already been approved for the treatment of other types of viral infection, as well as drugs that are currently used for entirely different purposes. High in vitro activity has been found for the nucleotide analogue remdesivir, for the antimalarial drug chloroquine, and for nitazoxanide, a drug used to treat protozoan infections. Because the virus enters human cells by way of the membrane-associated angiotensin converting enzyme 2 (ACE2), keeping the virus from docking to this receptor is a conceivable treatment approach. Transmembrane protease serine 2 (TMPRSS2) plays a role in the fusion of the virus with cells; inhibitors of this enzyme are known as well. The potential therapeutic efficacy and tolerability of these and other active substances remain to be investigated in clinical trials. At present, more than 80 trials on COVID-10 have already been registered with Clinical- Trials.gov. Some initial findings should already be available in late April 2020.
    Conclusion: Clinical trials are now indispensable in order to determine the true clinical benefits and risks of the substances that have been found to be active against SARSCoV- 2 in vitro. There is not yet any recommendation for the therapeutic use of any particular agent beyond standard supportive treatment.
    MeSH term(s) Antiviral Agents/therapeutic use ; Betacoronavirus ; COVID-19 ; Clinical Trials as Topic/statistics & numerical data ; Coronavirus Infections/drug therapy ; Drug Development/statistics & numerical data ; Humans ; Pandemics ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/virology ; SARS-CoV-2
    Chemical Substances Antiviral Agents
    Keywords covid19
    Language English
    Publishing date 2020-04-27
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2406159-1
    ISSN 1866-0452 ; 1866-0452
    ISSN (online) 1866-0452
    ISSN 1866-0452
    DOI 10.3238/arztebl.2020.0213
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Medication for COVID-19—an overview of approaches currently under study

    Stahlmann, Ralf / Lode, Hartmut

    Deutsches Aerzteblatt Online ; ISSN 1866-0452

    2020  

    Keywords General Medicine ; covid19
    Publisher Deutscher Arzte-Verlag GmbH
    Publishing country de
    Document type Article ; Online
    DOI 10.3238/arztebl.2020.0213
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Pille danach“ Wirkungsverlust durch Antibiotika?

    Häschke, Denise / Stahlmann, Ralf

    Medizinische Monatsschrift fur Pharmazeuten

    2018  Volume 39, Issue 12, Page(s) 528–530

    Title translation In process.
    MeSH term(s) Administration, Cutaneous ; Anti-Bacterial Agents/adverse effects ; Anti-Bacterial Agents/therapeutic use ; Contraceptive Agents, Female/administration & dosage ; Contraceptive Agents, Female/antagonists & inhibitors ; Contraceptive Effectiveness ; Contraceptives, Oral, Hormonal/antagonists & inhibitors ; Contraceptives, Oral, Hormonal/therapeutic use ; Contraceptives, Postcoital/antagonists & inhibitors ; Contraceptives, Postcoital/therapeutic use ; Drug Interactions ; Female ; Humans ; Pregnancy
    Chemical Substances Anti-Bacterial Agents ; Contraceptive Agents, Female ; Contraceptives, Oral, Hormonal ; Contraceptives, Postcoital
    Language German
    Publishing date 2018-07-30
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 132805-0
    ISSN 0342-9601
    ISSN 0342-9601
    Database MEDical Literature Analysis and Retrieval System OnLINE

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