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Book: Methods in biliary research

Muraca, Maurizio

1995

Author's details	ed. by Maurizio Muraca
Keywords	Biliary Tract / physiology ; Biliary Tract / physiopathology ; Gallengang ; Pathophysiologie
Subject	Pathologische Physiologie ; Physiologische Pathologie ; Physiopathologie
Language	English
Size	322 S. : Ill., graph. Darst.
Publisher	CRC Press
Publishing place	Boca Raton u.a.
Publishing country	United States
Document type	Book
HBZ-ID	HT006833954
ISBN	0-8493-8701-9 ; 978-0-8493-8701-2
Database	Catalogue ZB MED Medicine, Health

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Article ; Online: Circulating Extracellular Vesicles Express Receptor Activator of Nuclear Factor κB Ligand and Other Molecules Informative of the Bone Metabolic Status of Mouse Models of Experimentally Induced Osteoporosis.

Cappariello, Alfredo / Muraca, Maurizio / Teti, Anna / Rucci, Nadia

Calcified tissue international

2022 Volume 112, Issue 1, Page(s) 74–91

Abstract: Extracellular vesicles (EVs) are potent means of cell-to-cell communication. They are released in biological fluids, including blood, urine, and saliva, and can be exploited to identify new biomarkers of diseases. We hypothesized that EVs contain ... ...

Abstract	Extracellular vesicles (EVs) are potent means of cell-to-cell communication. They are released in biological fluids, including blood, urine, and saliva, and can be exploited to identify new biomarkers of diseases. We hypothesized that EVs contain molecular cargos involved in bone metabolism, possibly mirroring biological differences between postmenopausal and disuse osteoporosis. We tested this hypothesis in primary murine osteoblasts subjected to steroid depletion or to unloading, and in the serum of animal models of osteoporosis induced by ovariectomy or hindlimb tail suspension. EVs were isolated by ultracentrifugation and analysed by transmission electron microscopy, cytofluorimetry, immunoblotting and RT-PCR. Large-scale analyses were performed by Real-Time arrays and Proteome Profiler™ Antibody arrays. Finally, precise titration of analytes was carried out by ELISA assay. In vitro, we confirmed an increased release of EVs enriched in surface RANKL by primary mouse osteoblasts subjected to steroid depletion or simulated microgravity compared to controls. In vivo, circulating EVs isolated from the sera of control female mice expressed RANKL along with other genes associated with bone metabolism. Serum EVs from ovariectomized or hindlimb tail-suspended mice showed distinct molecular profiles. They expressed RANKL with different kinetics, while transcriptomic and proteomic profiles uncovered unique molecular signatures that discriminated the two conditions, unveiling exclusive molecules expressed in time- and osteoporosis type-dependent manner. These results suggest that circulating EVs could represent a new tool for monitoring the onset and the progression of diverse types of the disease in mice, paving the way for their exploitation to diagnose human osteoporosis in liquid biopsies.
MeSH term(s)	Female ; Mice ; Humans ; Animals ; NF-kappa B/metabolism ; RANK Ligand/metabolism ; Proteomics ; Cell Differentiation ; Osteoporosis/metabolism ; Bone Density Conservation Agents ; Extracellular Vesicles/metabolism
Chemical Substances	NF-kappa B ; RANK Ligand ; Bone Density Conservation Agents
Language	English
Publishing date	2022-10-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	304266-2
ISSN	1432-0827 ; 0944-0747 ; 0008-0594 ; 0171-967X
ISSN (online)	1432-0827
ISSN	0944-0747 ; 0008-0594 ; 0171-967X
DOI	10.1007/s00223-022-01032-5
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Article ; Online: The Role of Extracellular Vesicles (EVs) in the Epigenetic Regulation of Bone Metabolism and Osteoporosis.

Muraca, Maurizio / Cappariello, Alfredo

International journal of molecular sciences

2020 Volume 21, Issue 22

Abstract: Extracellular vesicles (EVs) are complex phospholipidic structures actively released by cells. EVs are recognized as powerful means of intercellular communication since they contain many signaling molecules (including lipids, proteins, and nucleic acids). ...

Abstract	Extracellular vesicles (EVs) are complex phospholipidic structures actively released by cells. EVs are recognized as powerful means of intercellular communication since they contain many signaling molecules (including lipids, proteins, and nucleic acids). In parallel, changes in epigenetic processes can lead to changes in gene function and finally lead to disease onset and progression. Recent breakthroughs have revealed the complex roles of non-coding RNAs (microRNAs (miRNAs) and long non-coding RNAs (lncRNAs)) in epigenetic regulation. Moreover, a substantial body of evidence demonstrates that non-coding RNAs can be shuttled among the cells and tissues via EVs, allowing non-coding RNAs to reach distant cells and exert systemic effects. Resident bone cells, including osteoclasts, osteoblasts, osteocytes, and endothelial cells, are tightly regulated by non-coding RNAs, and many of them can be exported from the cells to neighboring ones through EVs, triggering pathological conditions. For these reasons, researchers have also started to exploit EVs as a theranostic tool to address osteoporosis. In this review, we summarize some recent findings regarding the EVs' involvement in the fine regulation of non-coding RNAs in the context of bone metabolism and osteoporosis.
MeSH term(s)	Bone and Bones/metabolism ; Bone and Bones/pathology ; Epigenesis, Genetic ; Extracellular Vesicles/metabolism ; Extracellular Vesicles/pathology ; Humans ; MicroRNAs/metabolism ; Osteoporosis/metabolism ; Osteoporosis/pathology ; Osteoporosis/therapy ; RNA, Long Noncoding/metabolism
Chemical Substances	MicroRNAs ; RNA, Long Noncoding
Language	English
Publishing date	2020-11-17
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21228682
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Article ; Online: Extracellular Vesicles: A New Promise for the Prevention of Bronchopulmonary Dysplasia.

Bonadies, Luca / De Vos, Beatrice / Muraca, Maurizio / Baraldi, Eugenio

American journal of perinatology

2022 Volume 39, Issue S 01, Page(s) S23–S25

Abstract: Bronchopulmonary dysplasia (BPD) despite numerous efforts of neonatologists remains one of the most frequent and long-lasting chronic respiratory diseases consequent to extreme preterm birth. New clinical trials are exploring the possible use of ... ...

Abstract	Bronchopulmonary dysplasia (BPD) despite numerous efforts of neonatologists remains one of the most frequent and long-lasting chronic respiratory diseases consequent to extreme preterm birth. New clinical trials are exploring the possible use of mesenchymal stem cells (MSCs) and especially their products, extracellular vesicles (EVs), that overcome some of the possible issues related to the use of live cells. MSCs already reached clinical implementation; MSC-EVs, on the contrary, showed extremely promising results in the preclinical setting but are still waiting their first in human results that are likely to happen soon. KEY POINTS: · BPD is one of the most frequent complications of preterm birth, and its prevention lacks an effective tool.. · EVs have shown encouraging results in preclinical animal models.. · Technical and biological advancements are needed before routine clinical use..
MeSH term(s)	Animals ; Female ; Infant, Newborn ; Humans ; Bronchopulmonary Dysplasia/prevention & control ; Premature Birth ; Extracellular Vesicles ; Mesenchymal Stem Cells ; Disease Models, Animal ; Mesenchymal Stem Cell Transplantation/methods
Language	English
Publishing date	2022-11-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	605671-4
ISSN	1098-8785 ; 0735-1631
ISSN (online)	1098-8785
ISSN	0735-1631
DOI	10.1055/s-0042-1757351
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Article ; Online: Ion exchange chromatography as a simple and scalable method to isolate biologically active small extracellular vesicles from conditioned media.

Malvicini, Ricardo / Santa-Cruz, Diego / Tolomeo, Anna Maria / Muraca, Maurizio / Yannarelli, Gustavo / Pacienza, Natalia

PloS one

2023 Volume 18, Issue 9, Page(s) e0291589

Abstract: In the last few years, extracellular vesicles (EVs) have become of great interest due to their potential as biomarkers, drug delivery systems, and, in particular, therapeutic agents. However, there is no consensus on which is the best way to isolate ... ...

Abstract	In the last few years, extracellular vesicles (EVs) have become of great interest due to their potential as biomarkers, drug delivery systems, and, in particular, therapeutic agents. However, there is no consensus on which is the best way to isolate these EVs. The choice of the isolation method depends on the starting material (i.e., conditioned culture media, urine, serum, etc.) and their downstream applications. Even though there are numerous methods to isolate EVs, few are compatible with clinical applications as they are not scalable. In the present work, we set up a protocol to isolate EVs from conditioned media by ion exchange chromatography, a simple, fast, and scalable method, suitable for clinical production. We performed the isolation using an anion exchange resin (Q sepharose) and eluted the EVs using 500 mM NaCl. We characterized the elution profile by measuring protein and lipid concentration, and CD63 by ELISA. Moreover, we immunophenotyped all the eluted fractions, assessed the presence of TSG101, calnexin, and cytochrome C by western blot, analyzed nanoparticle size and distribution by tRPS, and morphology by TEM. Finally, we evaluated the immunomodulatory activity in vitro. We found that most EVs are eluted and concentrated in a single peak fraction, with a mean particle size of <150nm and expression of CD9, CD63, CD81, and TSG101 markers. Moreover, sEVs in fraction 4 exerted an anti-inflammatory activity on LPS-stimulated macrophages. In summary, we set up a chromatographic, scalable, and clinically compatible method to isolate and concentrate small EVs from conditioned media, which preserves the EVs biological activity.
MeSH term(s)	Culture Media, Conditioned/pharmacology ; Chromatography, Ion Exchange ; Blotting, Western ; Body Fluids ; Extracellular Vesicles
Chemical Substances	Culture Media, Conditioned
Language	English
Publishing date	2023-09-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0291589
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Article ; Online: The Role of Extracellular Vesicles (EVs) in the Epigenetic Regulation of Bone Metabolism and Osteoporosis

Maurizio Muraca / Alfredo Cappariello

International Journal of Molecular Sciences, Vol 21, Iss 8682, p

2020 Volume 8682

Abstract	Extracellular vesicles (EVs) are complex phospholipidic structures actively released by cells. EVs are recognized as powerful means of intercellular communication since they contain many signaling molecules (including lipids, proteins, and nucleic acids). In parallel, changes in epigenetic processes can lead to changes in gene function and finally lead to disease onset and progression. Recent breakthroughs have revealed the complex roles of non-coding RNAs (microRNAs (miRNAs) and long non-coding RNAs (lncRNAs)) in epigenetic regulation. Moreover, a substantial body of evidence demonstrates that non-coding RNAs can be shuttled among the cells and tissues via EVs, allowing non-coding RNAs to reach distant cells and exert systemic effects. Resident bone cells, including osteoclasts, osteoblasts, osteocytes, and endothelial cells, are tightly regulated by non-coding RNAs, and many of them can be exported from the cells to neighboring ones through EVs, triggering pathological conditions. For these reasons, researchers have also started to exploit EVs as a theranostic tool to address osteoporosis. In this review, we summarize some recent findings regarding the EVs’ involvement in the fine regulation of non-coding RNAs in the context of bone metabolism and osteoporosis.
Keywords	epigenetics ; long non-coding RNA ; microRNA ; osteoblast ; osteoclast ; osteocyte ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Thesis: Studies on bilirubin conjugation

Muraca, Maurizio

1985

Size	99 S. : graph. Darst.
Publishing country	Netherlands
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Leuven, Kathol. Univ., Diss., 1985
HBZ-ID	HT003063061
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

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88 E 188	order for loan	Magazin

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Article ; Online: Research Models to Mimic Necrotizing Enterocolitis and Inflammatory Bowel Diseases: Focus on Extracellular Vesicles Action.

Duci, Miriam / De Cesare, Ludovica / Hochuli, Agner Henrique Dorigo / Muraca, Maurizio / Cananzi, Mara / Gamba, Piergiorgio / Fascetti-Leon, Francesco / Pozzobon, Michela

Stem cells (Dayton, Ohio)

2023 Volume 41, Issue 12, Page(s) 1091–1100

Abstract: This review focuses on the crucial role of the intestinal epithelium in maintaining intestinal homeostasis and its significance in the pathogenesis of necrotizing enterocolitis (NEC) and inflammatory bowel diseases (IBD). NEC is a devastating neonatal ... ...

Abstract	This review focuses on the crucial role of the intestinal epithelium in maintaining intestinal homeostasis and its significance in the pathogenesis of necrotizing enterocolitis (NEC) and inflammatory bowel diseases (IBD). NEC is a devastating neonatal disease, while IBD represents a global healthcare problem with increasing incidence. The breakdown of the intestinal barrier in neonates is considered pivotal in the development and progression of both disorders. This review provides an overview of the current state of in vitro, ex vivo, and animal models to study epithelial injury in NEC and IBD, addressing pertinent questions that engage clinicians and researchers alike. Despite significant advancements in early recognition and aggressive treatment, no single therapy has been conclusively proven effective in reducing the severity of these disorders. Although early interventions have improved clinical outcomes, NEC and IBD continue to impose substantial morbidity, mortality, and economic burdens on affected individuals and society. Consequently, exploring alternative therapeutic options capable of preventing and treating the sequelae of NEC and IBD has become a pressing necessity. In recent decades, extracellular vehicles (EVs) have emerged as a potential solution to modulate the pathogenic mechanism in these multifactorial and complex disorders. Despite the diverse array of proposed models, a comprehensive model to investigate and decelerate the progression of NEC and IBD remains to be established. To bridge the translational gap between preclinical studies and clinical applications, enhancements in the technical development of gut-on-a-chip models and EVs hold considerable promise.
MeSH term(s)	Animals ; Infant, Newborn ; Humans ; Enterocolitis, Necrotizing/diagnosis ; Enterocolitis, Necrotizing/therapy ; Enterocolitis, Necrotizing/metabolism ; Inflammatory Bowel Diseases/metabolism ; Inflammatory Bowel Diseases/pathology ; Infant, Newborn, Diseases ; Disease Models, Animal ; Extracellular Vesicles/metabolism
Language	English
Publishing date	2023-09-08
Publishing country	England
Document type	Review ; Journal Article
ZDB-ID	1143556-2
ISSN	1549-4918 ; 1066-5099
ISSN (online)	1549-4918
ISSN	1066-5099
DOI	10.1093/stmcls/sxad068
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Article ; Online: Present and future are getting confused: are we equipped to face the technological revolution?

Perilongo, Giorgio / Agostiniani, Rino / Corsello, Giovanni / Marseglia, Gianluigi / Muraca, Maurizio / Staiano, Anna Maria / Zuccotti, Gianvincenzo / Baraldi, Eugenio

Italian journal of pediatrics

2023 Volume 49, Issue 1, Page(s) 164

Abstract: Background: This is a commentary reporting the outcome of a workshop promoted by the Department of Woman's and Child's Health of the University of Padua (Italy) focused on the emerging issue of what seems to be the increasing agemone role of technology.! ...

Abstract	Background: This is a commentary reporting the outcome of a workshop promoted by the Department of Woman's and Child's Health of the University of Padua (Italy) focused on the emerging issue of what seems to be the increasing agemone role of technology. Main body: Over the centuries, technology has always been at the service of science, with theoretical insights anticipating experimental proofs. Over the last decades, however, the situation has radically changed, due to several factors. Technology seems to be playing an agemone role. The present and notably the future generation of scientists have major challenges to face. They have to deal with the forces generated by the infosphera; to dominate the technology and to maintain the capacity of generating inquisitive, creative, ethical and spiritual thoughts capable of addressing new scientific hypotheses and projects directed to the individual and collective good. However, in this scenario, what seems more relevant is to focus all our efforts in preparing ourselves, first, and then the new generations to face these challenges. From this point of view, the academic institutions and the scientific societies, have a major responsibility to deal with. Conclusions: The academic ecosystem traditionally used to educate the new generation of professionals as well as, and most importantly, the cultural, the professional pathways presently used to form them need to be extensively revised. The time is running short and the stakes are high. The debate is open.
MeSH term(s)	Humans ; Italy ; Technology ; Forecasting
Language	English
Publishing date	2023-12-12
Publishing country	England
Document type	Letter
ZDB-ID	2088556-8
ISSN	1824-7288 ; 1720-8424
ISSN (online)	1824-7288
ISSN	1720-8424
DOI	10.1186/s13052-023-01529-1
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Zs.A 1961: Show issues

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Article ; Online: Evolving concepts in cell therapy of liver disease and current clinical perspectives.

Muraca, Maurizio

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

2011 Volume 43, Issue 3, Page(s) 180–187

Abstract: The clinical use of cells for the treatment of liver disease is not a mere hypothesis. Indeed, it has been known for more than 30 years that, following intraportal infusion, exogenous hepatocytes isolated from a donor liver engraft into the recipient ... ...

Abstract	The clinical use of cells for the treatment of liver disease is not a mere hypothesis. Indeed, it has been known for more than 30 years that, following intraportal infusion, exogenous hepatocytes isolated from a donor liver engraft into the recipient hepatic parenchyma and express metabolic activity. These experimental results encouraged pilot clinical trials using hepatocytes transplantation to treat a variety of liver diseases. More recently, the discovery of liver stem/progenitor cells further fueled the interest in the field. However, it appears that successful liver cell therapy will require better understanding of the mechanisms governing liver regeneration and of their implication in cell transplantation. This review summarizes some recent advances in the field in a bench-to-bedside perspective.
MeSH term(s)	Bone Marrow Transplantation ; Hematopoietic Stem Cell Transplantation ; Hepatocytes/transplantation ; Humans ; Liver Diseases/surgery ; Liver Regeneration ; Mesenchymal Stem Cell Transplantation ; Multipotent Stem Cells/transplantation ; Stem Cell Transplantation
Language	English
Publishing date	2011-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1459373-7
ISSN	1878-3562 ; 1125-8055
ISSN (online)	1878-3562
ISSN	1125-8055
DOI	10.1016/j.dld.2010.08.007
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