Article ; Online: T
Chemical communications (Cambridge, England)
2020 Volume 56, Issue 74, Page(s) 10890–10893
Abstract: Modulating the phase-memory relaxation time (Tm) of a spin label by introducing 1H-methyl groups in a perdeuterated protein background is used in DEER experiments to assign interactions in multimodal P(r) distributions. Proof of principle is demonstrated ...
| Abstract | Modulating the phase-memory relaxation time (Tm) of a spin label by introducing 1H-methyl groups in a perdeuterated protein background is used in DEER experiments to assign interactions in multimodal P(r) distributions. Proof of principle is demonstrated using Protein A where one nitroxide label occupies two distinct regions of conformational space. The presence of a single protonated methyl group in close proximity (4-8 Å) to only one of the two nitroxide rotamer ensembles results in a selective and substantial decrease in Tm, manifested by differential decay of the peak intensities in the bimodal P(r) distance distribution as a function of the total dipolar evolution time. This form of Tm filtering will facilitate DEER structural analysis of biomolecular systems with three spin labels, including complexes involving multimeric proteins. |
|---|---|
| MeSH term(s) | Electron Spin Resonance Spectroscopy ; Electrons ; Protons ; Spin Labels ; Staphylococcal Protein A/chemistry ; Time Factors |
| Chemical Substances | Protons ; Spin Labels ; Staphylococcal Protein A |
| Language | English |
| Publishing date | 2020-09-16 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 1472881-3 |
| ISSN | 1364-548X ; 1359-7345 ; 0009-241X |
| ISSN (online) | 1364-548X |
| ISSN | 1359-7345 ; 0009-241X |
| DOI | 10.1039/d0cc04369a |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.