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Article: Photoaffinity inhibition of rat liver NAD(P)H dehydrogenase by 3-(alpha-acetonyl-p-azidobenzyl)-4-hydroxycoumarin.

Almeda, S / Bing, D H / Laura, R / Friedman, P A

1981 Volume 20, Issue 13, Page(s) 3731–3737

Abstract: NAD(P)H dehydrogenase was purified in four steps from a homogenate of rat liver. The final step was ... inhibited competitively with respect to NADH by 3-(alpha-acetonyl-p-nitrobenzyl)-4-hydroxycoumarin ...

Abstract	NAD(P)H dehydrogenase was purified in four steps from a homogenate of rat liver. The final step was affinity chromatography on Sepharose coupled to 3,3'-(m-hydroxybenzylidene)bis(4-hydroxycoumarin). The purified enzyme was inhibited competitively with respect to NADH by 3-(alpha-acetonyl-p-nitrobenzyl)-4-hydroxycoumarin (acenocoumarin) (Ki = 1.7 microM). The acenocoumarin was converted into an azide which was used to photoaffinity inhibit the enzyme. Following photolysis in the presence of the azide, the enzyme was inactivated in proportion to the concentration of azide present during irradiation. A maximum of 35-40% inhibition could be achieved by a single irradiation at 254 nm for 1.5 min. This inhibition was noncompetitive with respect to NADH. The inactivation was shown to be specific as acenocoumarin afforded complete protection against inactivation, irradiation was required to achieve inactivation, and the enzyme was unaffected by irradiation alone.
MeSH term(s)	Acenocoumarol/pharmacology ; Affinity Labels/pharmacology ; Animals ; Azides ; Chromatography, Affinity ; Liver/enzymology ; NADH, NADPH Oxidoreductases/antagonists & inhibitors ; NADPH Dehydrogenase/antagonists & inhibitors ; NADPH Dehydrogenase/isolation & purification ; NADPH Dehydrogenase/radiation effects ; Photolysis ; Rats ; Warfarin/analogs & derivatives ; Warfarin/pharmacology
Chemical Substances	Affinity Labels ; Azides ; Warfarin (5Q7ZVV76EI) ; azidowarfarin (77741-79-8) ; NADH, NADPH Oxidoreductases (EC 1.6.-) ; NADPH Dehydrogenase (EC 1.6.99.1) ; Acenocoumarol (I6WP63U32H)
Language	English
Publishing date	1981-06-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1108-3
ISSN	1520-4995 ; 0006-2960
ISSN (online)	1520-4995
ISSN	0006-2960
DOI	10.1021/bi00516a010
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Zs.A 217: Show issues

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Article ; Online: Perceived ankle instability and cutaneous reflex modulation during gait.

Friedman, Annalee M H / Madsen, Leif P

Physiological reports

2023 Volume 11, Issue 22, Page(s) e15880

Abstract: Cutaneous reflex modulation during rhythmic ambulation is an important motor control mechanism to help minimize stumbling following an unexpected perturbation. Previous literature found individuals with chronic ankle instability (CAI) experience altered ... ...

Abstract	Cutaneous reflex modulation during rhythmic ambulation is an important motor control mechanism to help minimize stumbling following an unexpected perturbation. Previous literature found individuals with chronic ankle instability (CAI) experience altered reflex patterns compared to healthy controls. Considering CAI is characterized by intermittent feelings of ankle instability, researchers have speculated that these alterations are related to perceived instability. Our purpose was to determine whether variability and magnitude of cutaneous reflex amplitudes can predict perceived instability levels following sural nerve stimulation during gait. Forty subjects walked while receiving random stimulations and reported their perceived instability. Middle latency reflexes among lower leg muscles were calculated using data derived from surface electromyography. Hierarchical logistical regressions revealed a positive relationship between reflex variability of the peroneus longus and lateral gastrocnemius muscles and perceived instability during midstance. This suggests subjects with consistent reflexes following sural nerve stimulation develop a certain level of perceptual expectation resulting in generally lower feelings of ankle instability, while subjects with more variable motor outputs perceive greater instability at the supraspinal level. Cutaneous reflex variability during stance may be an important objective outcome measure to monitor neuromuscular recovery throughout a rehabilitation or as a potential predictor of future lateral ankle sprains.
MeSH term(s)	Humans ; Ankle ; Ankle Joint ; Gait/physiology ; Muscle, Skeletal/physiology ; Electromyography/methods ; Reflex/physiology ; Joint Instability
Language	English
Publishing date	2023-11-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2724325-4
ISSN	2051-817X ; 2051-817X
ISSN (online)	2051-817X
ISSN	2051-817X
DOI	10.14814/phy2.15880
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Article ; Online: Cardiac timing and threatening stimuli influence response inhibition and ex-Gaussian parameters of reaction time in a Go/No-go task.

Yang, Xiao / Spangler, Derek P / Jennings, J Richard / Friedman, Bruce H

Psychophysiology

2023 Volume 60, Issue 6, Page(s) e14260

Abstract: Sensorimotor responses vary as a function of the cardiac cycle phase. These effects, known as cardiac cycle time effects, have been explained by the inhibition of cardiac afferent signals on information processing. However, the validity of cardiac cycle ... ...

Abstract	Sensorimotor responses vary as a function of the cardiac cycle phase. These effects, known as cardiac cycle time effects, have been explained by the inhibition of cardiac afferent signals on information processing. However, the validity of cardiac cycle time effects is challenged by mixed findings. Factors such as current information processing and affective context may modulate cardiac cycle time effects and account for inconsistencies in the literature. The current study examines the influence of cardiac cycle time and threatening stimuli on two aspects of sensorimotor processing, response speed and inhibition. Thirty-four participants (M
MeSH term(s)	Fear/physiology ; Humans ; Male ; Female ; Young Adult ; Reaction Time ; Heart/physiology ; Systole ; Diastole ; Feedback, Sensory ; Facial Expression ; Time Factors ; Inhibition, Psychological
Language	English
Publishing date	2023-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	209486-1
ISSN	1469-8986 ; 1540-5958 ; 0048-5772
ISSN (online)	1469-8986 ; 1540-5958
ISSN	0048-5772
DOI	10.1111/psyp.14260
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Zs.A 502: Show issues

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Article ; Online: The role of sural nerve reflexes during drop-landing in subjects with and without chronic ankle instability.

Heimark, Nicole E / Friedman, Annalee M H / Kitano, Koichi / Madsen, Leif P

Experimental brain research

2023 Volume 241, Issue 6, Page(s) 1691–1705

Abstract: ... experienced significantly more inhibition of the PL compared to the CAI group (p = 0.019). These results ...

Abstract	The purpose of this study was to investigate the functional role of cutaneous reflexes during a single-leg drop-landing task among healthy, neurologically intact adults, and to identify whether individuals with chronic ankle instability (CAI) demonstrate altered reflexes and subsequent ankle kinematics. All subjects were physically active adults and were categorized as control (n = 10, Male = 6, Female = 4) or CAI (n = 9, Male = 4, Female = 5) depending on whether they scored a 0 or ≥ 11 on the Identification of Functional Ankle Instability questionnaire, respectively. Subjects performed 30-40 single-leg drop-landing trials from a platform set to the height of their tibial tuberosity. Muscle activity of four lower leg muscles was collected via surface electromyography, while ankle kinematics were recorded via an electrogoniometer. Non-noxious stimulations were elicited randomly to the ipsilateral sural nerve at two unique phases of the drop-landing task (takeoff and landing). Unstimulated and stimulated trials were used to calculate middle latency reflex amplitudes (80-120 ms) and net ankle kinematics (140-220 ms) post-stimulation. Mixed-factor ANOVAs were used to identify significant reflexes within groups and differences in reflex amplitudes between groups. Unlike the CAI group, the control group experienced significant facilitation of the Peroneus Longus (PL) and inhibition of the Lateral Gastrocnemius (LG) when stimulated at takeoff, resulting in eversion immediately prior to landing. When stimulated at landing, the control group experienced significantly more inhibition of the PL compared to the CAI group (p = 0.019). These results suggest lower neural excitability for individuals with CAI, which may predispose them to recurrent injury during similar functional tasks.
MeSH term(s)	Adult ; Female ; Humans ; Male ; Ankle ; Ankle Joint ; Biomechanical Phenomena ; Electromyography/methods ; Joint Instability ; Muscle, Skeletal/physiology ; Reflex/physiology ; Sural Nerve ; Case-Control Studies
Language	English
Publishing date	2023-05-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1201-4
ISSN	1432-1106 ; 0014-4819
ISSN (online)	1432-1106
ISSN	0014-4819
DOI	10.1007/s00221-023-06636-3
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Zs.A 278: Show issues

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Article: Perceptions, Utilization, and Cost Assessment of Sebaceous Hyperplasia Treatment Modalities: A Pilot Survey.

Millan, S H / Sellyn, G E / Friedman, Adam J

Journal of drugs in dermatology : JDD

2024 Volume 23, Issue 2, Page(s) 29–37

Abstract: ... were used more than pharmacologic treatments (P=<0.05). Treatments most used by respondents included ...

Abstract	Background: Sebaceous hyperplasia (SH) is a common, benign but cosmetically bothersome skin condition preferentially affecting older adults. Despite multiple treatment options, there is no universally accepted first-line treatment for SH nor standard pricing for said approaches. Methods: A survey aimed at evaluating treatment approaches and their respective costs was disseminated on the Orlando Dermatology Aesthetic and Clinical Conference email listserv. Results: Out of 224 dermatologists who participated in the survey (response rate 9.2%), most treated patients with SH (95.98%). In-office procedures were used more than pharmacologic treatments (P=<0.05). Treatments most used by respondents included electrodesiccation (ED; 83.9%), cryosurgery (35.3%), oral isotretinoin (32.6%), and carbon dioxide (CO2) laser (19.2%). Cryosurgery and ED priced between <$200 to $400. Most reported 1 to 2 sessions to achieve lesion clearance for ED, CO2 laser, and cryosurgery. Twenty-one percent reported 3-4 sessions with cryosurgery. Chemical peels, diode lasers, and photodynamic therapy required between 2-4 sessions. Respondents indicated lesions were most unlikely to recur with ED and CO2 laser. Most dermatologists (86.39%) agreed or strongly agreed that they were exposed to new treatments methods for SH through this survey and 86.49% of dermatologists were interested in learning about treatments employed by others. Conclusion: SH is a common issue that presents in the dermatologist's office. These data highlight the perception that ED is the most common approach employed, associated with lower costs, and requiring fewer sessions to achieve resolution. More data is needed and wanted to better determine best practices for the management of SH.J Drugs Dermatol. 2024;23(2):29-37. doi:10.36849/JDD.7734.
MeSH term(s)	Humans ; Aged ; Hyperplasia/therapy ; Sebaceous Gland Diseases/pathology ; Photochemotherapy/methods ; Dermabrasion ; Surveys and Questionnaires
Language	English
Publishing date	2024-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2145090-0
ISSN	1545-9616
ISSN	1545-9616
DOI	10.36849/JDD.7734
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Zs.A 5931: Show issues

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Article ; Online: Deaths of despair and Indigenous data genocide.

Friedman, Joseph / Hansen, Helena / Gone, Joseph P

Lancet (London, England)

2023 Volume 401, Issue 10379, Page(s) 874–876

MeSH term(s)	Humans ; Genocide ; Homicide ; Rwanda
Language	English
Publishing date	2023-01-26
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	3306-6
ISSN	1474-547X ; 0023-7507 ; 0140-6736
ISSN (online)	1474-547X
ISSN	0023-7507 ; 0140-6736
DOI	10.1016/S0140-6736(22)02404-7
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Ua VI Zs.5: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 94: Show issues

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Article ; Online: The Search for Genomic Biomarkers of Response to Immunotherapy in Ovarian Cancer.

Al-Rawi, Duaa H / Rusk, Nicole / Friedman, Claire F

Clinical cancer research : an official journal of the American Association for Cancer Research

2023 Volume 29, Issue 9, Page(s) 1645–1647

Abstract: ... progression-free survival with the addition of the PD-L1 inhibitor atezolizumab. See related article by Landen et al., p ...

Abstract	Immune checkpoint blockade has been ineffective in ovarian cancer, and there is an ongoing effort to identify biomarkers of therapeutic benefit. Despite promising preclinical data, a substudy of the IMagyn050 trial found that patients with homologous recombination deficient tumors did not have improved progression-free survival with the addition of the PD-L1 inhibitor atezolizumab. See related article by Landen et al., p. 1698.
MeSH term(s)	Humans ; Female ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/genetics ; Biomarkers, Tumor/genetics ; Immunotherapy ; Progression-Free Survival ; Genomics
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2023-02-23
Publishing country	United States
Document type	Editorial ; Research Support, N.I.H., Extramural ; Comment
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-23-0048
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Zs.A 4223: Show issues

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Article ; Online: Experimental Entomology in the Age of Video.

Friedman, Daniel A / Wexler, Judith R / Alvarado, Sebastian

Journal of visualized experiments : JoVE

2023 , Issue 192

Abstract: ... 169), e62316 (2022). Topitzhofer, E., Lucas, H., Carlson, E., Chakrabarti, P., Sagili, R. Collection ...

Abstract	Articles discussed: Smodiš Škerl, M. I. Histology basics and cell death detection in honeybee tissue. Journal of Visualized Experiments. (185), e64141 (2022). Fine, J. D., Torres, K. M., Martin, J., Robinson, G. E. Assessing agrochemical risk to mated honey bee queens. Journal of Visualized Experiments. (169), e62316 (2022). Topitzhofer, E., Lucas, H., Carlson, E., Chakrabarti, P., Sagili, R. Collection and identification of pollen from honey bee colonies. Journal of Visualized Experiments. (167), e62064 (2022). Nogueira, B. R., de Oliveira, A. A., Silva, D., Pereira da Silva, J., Bueno, O. C. Collection and long-term maintenance of leaf-cutting ants (Atta) in laboratory conditions. Journal of Visualized Experiments. (186), e64154 (2022).
MeSH term(s)	Animals ; Bees ; Agrochemicals ; Ants ; Cell Death ; Histological Techniques ; Laboratories
Chemical Substances	Agrochemicals
Language	English
Publishing date	2023-02-10
Publishing country	United States
Document type	Editorial ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/65002
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Article: Meta-analyses of the efficacy of pharmacotherapies and sublingual allergy immunotherapy tablets for allergic rhinitis in adults and children.

Meltzer, E O / Wallace, D / Friedman, H S / Navaratnam, P / Scott, E P / Nolte, H

Rhinology

2021 Volume 59, Issue 5, Page(s) 422–432

Abstract: Background: Treatment options for seasonal and perennial allergic rhinitis (SAR/PAR) include pharmacotherapies and allergy immunotherapy. These meta-analyses evaluated the efficacy of pharmacotherapies and sublingual immunotherapy tablets (SLIT-tablets) ...

Abstract	Background: Treatment options for seasonal and perennial allergic rhinitis (SAR/PAR) include pharmacotherapies and allergy immunotherapy. These meta-analyses evaluated the efficacy of pharmacotherapies and sublingual immunotherapy tablets (SLIT-tablets) versus placebo on nasal symptoms associated with SAR and PAR. Methods: Randomized, double-blind, placebo-controlled trials were identified from systematic PubMED/EMBASE searches through 7/18/2019 (PROSPERO protocol CRD42018105632). The primary outcome was mean numerical difference in total nasal symptom score (TNSS; 0-12) between active treatment and placebo at the end of the assessment period. Random-effects meta-analyses estimated the mean difference for each medication group weighted by the inverse of the trial variance. Publication bias assessments and sensitivity analyses were conducted. Results: Rescue symptom-relieving pharmacotherapy was prohibited in most pharmacotherapy trials but was allowed in all SLIT-tablet trials. For adult/adolescent SAR, the mean numerical difference (95% CI) in TNSS versus placebo was: intranasal corticosteroids (INCS)=1.38 (1.18, 1.58; 39 trials); combination intranasal antihistamine/INCS=1.34 (1.15, 1.54; 4 trials); intranasal antihistamines=0.72 (0.56, 0.89; 13 trials); oral antihistamine=0.62 (0.35, 0.90; 18 trials); SLIT-tablets=0.57 (0.41, 0.73; 4 trials); and montelukast=0.48 (0.36, 0.60; 10 trials). For adult/adolescent PAR, mean difference in TNSS versus placebo (95% CI) was: INCS=0.82 (0.66, 0.97; 14 trials); SLIT-tablets=0.65 (0.42, 0.88; 3 trials); and oral antihistamine=0.27 (0.11, 0.42; 3 trials). The number of eligible trials limited meta-analyses for pediatric SAR/PAR. Conclusions: All treatments significantly improved nasal symptoms versus placebo. SLIT-tablets provided improvement in TNSS despite access to rescue symptom-relieving pharmacotherapy. Extensive trial heterogeneity and strong indications of publication bias preclude the comparison of treatment effects among treatment classes.
MeSH term(s)	Administration, Sublingual ; Adolescent ; Adult ; Child ; Double-Blind Method ; Humans ; Rhinitis, Allergic/drug therapy ; Rhinitis, Allergic, Seasonal ; Sublingual Immunotherapy ; Tablets/therapeutic use ; Treatment Outcome
Chemical Substances	Tablets
Language	English
Publishing date	2021-09-14
Publishing country	Netherlands
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	80336-4
ISSN	0300-0729
ISSN	0300-0729
DOI	10.4193/Rhin21.054
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Zs.A 1080: Show issues

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Article ; Online: Ethics Consultation Requests After Implementation of an Electronic Health Record Order.

Marathe, Priya H / Zhang, Hao / Blackler, Liz / Stetson, Peter D / Voigt, Louis P / Friedman, Danielle Novetsky

JCO oncology practice

2022 Volume 18, Issue 9, Page(s) e1505–e1512

Abstract: Purpose: As the role of clinical ethics consultation in health care advances, there are calls to standardize the process of consultation. The Ethics Committee at Memorial Sloan Kettering Cancer Center (MSK) hypothesized that the process of requesting an ...

Abstract	Purpose: As the role of clinical ethics consultation in health care advances, there are calls to standardize the process of consultation. The Ethics Committee at Memorial Sloan Kettering Cancer Center (MSK) hypothesized that the process of requesting an ethics consultation could be improved by instituting an electronic health record (EHR) order for consultation requests. This report summarizes the impact of adopting an EHR order for ethics consultation requests at MSK. Methods: This retrospective review of all clinical ethics consultations requested at a tertiary cancer center from May 2017 to February 2020 spans 17 months before and after implementation of an electronic order for consultation requests. Summary statistics are presented using Pearson chi-square analyses with a significance level of 0.05. Results: There was a significant increase in the total number of consultation requests placed after implementation of the EHR order (n = 165, 0.08% of total patients) compared with before (n = 108, 0.05% of total patients; Conclusion: In this large single-institution retrospective study, implementation of an EHR order for ethics consultation requests was associated with a significant increase in the number of consultation requests. Implementation of an electronic order may decrease barriers to ethics consultation in diverse practice settings. Further longitudinal, multicenter studies are needed to assess strategies to improve access to clinical ethics consultation for oncology patients.
MeSH term(s)	Delivery of Health Care ; Electronic Health Records ; Ethics Committees ; Ethics Consultation ; Humans ; Retrospective Studies
Language	English
Publishing date	2022-06-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	3028198-2
ISSN	2688-1535 ; 2688-1527
ISSN (online)	2688-1535
ISSN	2688-1527
DOI	10.1200/OP.22.00174
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